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Protein

Golgi to ER traffic protein 2

Gene

GET2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Together with GET1, acts as a membrane receptor for soluble GET3, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. The GET complex cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in DNA replication and DNA damage response and also in cell wall function.UniRule annotation7 Publications

GO - Molecular functioni

  • protein anchor Source: SGD

GO - Biological processi

  • protein insertion into ER membrane Source: SGD
  • retrograde vesicle-mediated transport, Golgi to ER Source: SGD
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30253-MONOMER.

Protein family/group databases

TCDBi3.A.21.1.1. the c-terminal tail-anchored membrane protein biogenesis/ insertion complex (tamp-b) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi to ER traffic protein 2UniRule annotation
Alternative name(s):
Guided entry of tail-anchored proteins 2
Hydroxyurea resistance protein 2UniRule annotation
Required for meiotic nuclear division protein 7UniRule annotation
Gene namesi
Name:GET2UniRule annotation
Synonyms:HUR2UniRule annotation, RMD7UniRule annotation
Ordered Locus Names:YER083C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER083C.
SGDiS000000885. GET2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 148CytoplasmicUniRule annotationAdd BLAST147
Transmembranei149 – 169HelicalUniRule annotationAdd BLAST21
Topological domaini170 – 196LumenalUniRule annotationAdd BLAST27
Transmembranei197 – 216HelicalUniRule annotationAdd BLAST20
Topological domaini217 – 263CytoplasmicUniRule annotationAdd BLAST47
Transmembranei264 – 284HelicalUniRule annotationAdd BLAST21
Topological domaini285LumenalUniRule annotation1

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • GET complex Source: SGD
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000973622 – 285Golgi to ER traffic protein 2Add BLAST284

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei45PhosphoserineCombined sources1
Glycosylationi173N-linked (GlcNAc...)UniRule annotation1
Glycosylationi196N-linked (GlcNAc...)UniRule annotation1

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP40056.
PRIDEiP40056.
TopDownProteomicsiP40056.

PTM databases

iPTMnetiP40056.

Interactioni

Subunit structurei

Component of the Golgi to ER traffic (GET) complex, which is composed of GET1, GET2 and GET3.UniRule annotation4 Publications

GO - Molecular functioni

  • protein anchor Source: SGD

Protein-protein interaction databases

BioGridi36828. 697 interactors.
DIPiDIP-4508N.
IntActiP40056. 9 interactors.
MINTiMINT-501836.

Structurei

Secondary structure

1285
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 21Combined sources16
Turni22 – 24Combined sources3
Helixi25 – 33Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SJDX-ray4.60D/E2-35[»]
3ZS9X-ray2.10C/D1-38[»]
ProteinModelPortaliP40056.
SMRiP40056.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40056.

Family & Domainsi

Sequence similaritiesi

Belongs to the GET2 family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000066100.
InParanoidiP40056.
OMAiIDEMFKK.
OrthoDBiEOG092C45HY.

Family and domain databases

HAMAPiMF_03114. Get2. 1 hit.
InterProiIPR014802. GET2.
IPR028143. Get2/sif1.
[Graphical view]
PfamiPF08690. GET2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40056-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSELTEAEKR RLLRERRQKK FSNGGASSRL NKITGQASSH LNAESPLDAP
60 70 80 90 100
SAAKTTPPAS VHSATPDIKE DSNVAPQLDL LKQLAAMQGQ GTGKSTPQDS
110 120 130 140 150
STPDLLSLLS SMNTGMPSAE GTPSFGQAAP AAPINQAALD YHDYLLNRLK
160 170 180 190 200
AWTILVKWVF FLLPYLYLIT RPNSSVWPAY AFTQSAWFAP LRNPSNFTRI
210 220 230 240 250
FATFEFLSIS IYYQLLKNVE HKSKIKNLQD TNKLVKLVSL VPEGVIPVAN
260 270 280
LKGKLITLLQ YWDLLSMLIT DISFVLIVLG LLTYL
Length:285
Mass (Da):31,493
Last modified:May 16, 2006 - v2
Checksum:i1667E7C879CFB6EA
GO

Sequence cautioni

The sequence AAB64638 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18839 Genomic DNA. Translation: AAB64638.1. Different initiation.
BK006939 Genomic DNA. Translation: DAA07744.1.
PIRiS50586.
RefSeqiNP_011006.2. NM_001178974.1.

Genome annotation databases

EnsemblFungiiYER083C; YER083C; YER083C.
GeneIDi856817.
KEGGisce:YER083C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18839 Genomic DNA. Translation: AAB64638.1. Different initiation.
BK006939 Genomic DNA. Translation: DAA07744.1.
PIRiS50586.
RefSeqiNP_011006.2. NM_001178974.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SJDX-ray4.60D/E2-35[»]
3ZS9X-ray2.10C/D1-38[»]
ProteinModelPortaliP40056.
SMRiP40056.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36828. 697 interactors.
DIPiDIP-4508N.
IntActiP40056. 9 interactors.
MINTiMINT-501836.

Protein family/group databases

TCDBi3.A.21.1.1. the c-terminal tail-anchored membrane protein biogenesis/ insertion complex (tamp-b) family.

PTM databases

iPTMnetiP40056.

Proteomic databases

MaxQBiP40056.
PRIDEiP40056.
TopDownProteomicsiP40056.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER083C; YER083C; YER083C.
GeneIDi856817.
KEGGisce:YER083C.

Organism-specific databases

EuPathDBiFungiDB:YER083C.
SGDiS000000885. GET2.

Phylogenomic databases

HOGENOMiHOG000066100.
InParanoidiP40056.
OMAiIDEMFKK.
OrthoDBiEOG092C45HY.

Enzyme and pathway databases

BioCyciYEAST:G3O-30253-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP40056.
PROiP40056.

Family and domain databases

HAMAPiMF_03114. Get2. 1 hit.
InterProiIPR014802. GET2.
IPR028143. Get2/sif1.
[Graphical view]
PfamiPF08690. GET2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGET2_YEAST
AccessioniPrimary (citable) accession number: P40056
Secondary accession number(s): D3DLZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: May 16, 2006
Last modified: November 2, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.