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P40056

- GET2_YEAST

UniProt

P40056 - GET2_YEAST

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Protein

Golgi to ER traffic protein 2

Gene
GET2, HUR2, RMD7, YER083C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Together with GET1, acts as a membrane receptor for soluble GET3, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. The GET complex cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in DNA replication and DNA damage response and also in cell wall function.7 Publications

GO - Molecular functioni

  1. protein anchor Source: SGD

GO - Biological processi

  1. protein insertion into ER membrane Source: SGD
  2. retrograde vesicle-mediated transport, Golgi to ER Source: SGD
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30253-MONOMER.

Protein family/group databases

TCDBi3.A.21.1.1. the c-terminal tail-anchored membrane protein biogenesis/ insertion complex (tamp-b) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi to ER traffic protein 2
Alternative name(s):
Guided entry of tail-anchored proteins 2
Hydroxyurea resistance protein 2
Required for meiotic nuclear division protein 7
Gene namesi
Name:GET2
Synonyms:HUR2, RMD7
Ordered Locus Names:YER083C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER083c.
SGDiS000000885. GET2.

Subcellular locationi

Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein 4 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 148147Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei149 – 16921Helical; Reviewed predictionAdd
BLAST
Topological domaini170 – 19627Lumenal Reviewed predictionAdd
BLAST
Transmembranei197 – 21620Helical; Reviewed predictionAdd
BLAST
Topological domaini217 – 26347Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei264 – 28421Helical; Reviewed predictionAdd
BLAST
Topological domaini285 – 2851Lumenal Reviewed prediction

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. GET complex Source: SGD
  3. Golgi membrane Source: UniProtKB-SubCell
  4. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 285284Golgi to ER traffic protein 2UniRule annotationPRO_0000097362Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei45 – 451Phosphoserine1 Publication
Glycosylationi173 – 1731N-linked (GlcNAc...) Reviewed prediction
Glycosylationi196 – 1961N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP40056.
PaxDbiP40056.
PeptideAtlasiP40056.

Expressioni

Gene expression databases

GenevestigatoriP40056.

Interactioni

Subunit structurei

Component of the Golgi to ER traffic (GET) complex, which is composed of GET1, GET2 and GET3.4 Publications

Protein-protein interaction databases

BioGridi36828. 697 interactions.
DIPiDIP-4508N.
IntActiP40056. 9 interactions.
MINTiMINT-501836.
STRINGi4932.YER083C.

Structurei

Secondary structure

1
285
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 2116
Turni22 – 243
Helixi25 – 339

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3SJDX-ray4.60D/E2-35[»]
3ZS9X-ray2.10C/D1-38[»]
ProteinModelPortaliP40056.
SMRiP40056. Positions 4-38.

Miscellaneous databases

EvolutionaryTraceiP40056.

Family & Domainsi

Sequence similaritiesi

Belongs to the GET2 family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG38846.
HOGENOMiHOG000066100.
OMAiIDEMFKK.
OrthoDBiEOG708WB5.

Family and domain databases

HAMAPiMF_03114. Get2.
InterProiIPR014802. GET2.
IPR028143. Get2/sif1.
[Graphical view]
PfamiPF08690. GET2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40056-1 [UniParc]FASTAAdd to Basket

« Hide

MSELTEAEKR RLLRERRQKK FSNGGASSRL NKITGQASSH LNAESPLDAP    50
SAAKTTPPAS VHSATPDIKE DSNVAPQLDL LKQLAAMQGQ GTGKSTPQDS 100
STPDLLSLLS SMNTGMPSAE GTPSFGQAAP AAPINQAALD YHDYLLNRLK 150
AWTILVKWVF FLLPYLYLIT RPNSSVWPAY AFTQSAWFAP LRNPSNFTRI 200
FATFEFLSIS IYYQLLKNVE HKSKIKNLQD TNKLVKLVSL VPEGVIPVAN 250
LKGKLITLLQ YWDLLSMLIT DISFVLIVLG LLTYL 285
Length:285
Mass (Da):31,493
Last modified:May 16, 2006 - v2
Checksum:i1667E7C879CFB6EA
GO

Sequence cautioni

The sequence AAB64638.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18839 Genomic DNA. Translation: AAB64638.1. Different initiation.
BK006939 Genomic DNA. Translation: DAA07744.1.
PIRiS50586.
RefSeqiNP_011006.2. NM_001178974.1.

Genome annotation databases

EnsemblFungiiYER083C; YER083C; YER083C.
GeneIDi856817.
KEGGisce:YER083C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18839 Genomic DNA. Translation: AAB64638.1 . Different initiation.
BK006939 Genomic DNA. Translation: DAA07744.1 .
PIRi S50586.
RefSeqi NP_011006.2. NM_001178974.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3SJD X-ray 4.60 D/E 2-35 [» ]
3ZS9 X-ray 2.10 C/D 1-38 [» ]
ProteinModelPortali P40056.
SMRi P40056. Positions 4-38.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36828. 697 interactions.
DIPi DIP-4508N.
IntActi P40056. 9 interactions.
MINTi MINT-501836.
STRINGi 4932.YER083C.

Protein family/group databases

TCDBi 3.A.21.1.1. the c-terminal tail-anchored membrane protein biogenesis/ insertion complex (tamp-b) family.

Proteomic databases

MaxQBi P40056.
PaxDbi P40056.
PeptideAtlasi P40056.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER083C ; YER083C ; YER083C .
GeneIDi 856817.
KEGGi sce:YER083C.

Organism-specific databases

CYGDi YER083c.
SGDi S000000885. GET2.

Phylogenomic databases

eggNOGi NOG38846.
HOGENOMi HOG000066100.
OMAi IDEMFKK.
OrthoDBi EOG708WB5.

Enzyme and pathway databases

BioCyci YEAST:G3O-30253-MONOMER.

Miscellaneous databases

EvolutionaryTracei P40056.
NextBioi 983091.

Gene expression databases

Genevestigatori P40056.

Family and domain databases

HAMAPi MF_03114. Get2.
InterProi IPR014802. GET2.
IPR028143. Get2/sif1.
[Graphical view ]
Pfami PF08690. GET2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: FUNCTION.
  4. Cited for: IDENTIFICATION IN GET COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  5. "Large-scale functional genomic analysis of sporulation and meiosis in Saccharomyces cerevisiae."
    Enyenihi A.H., Saunders W.S.
    Genetics 163:47-54(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
    Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
    Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. "Novel functions of the phosphatidylinositol metabolic pathway discovered by a chemical genomics screen with wortmannin."
    Zewail A., Xie M.W., Xing Y., Lin L., Zhang P.F., Zou W., Saxe J.P., Huang J.
    Proc. Natl. Acad. Sci. U.S.A. 100:3345-3350(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Exploration of the function and organization of the yeast early secretory pathway through an epistatic miniarray profile."
    Schuldiner M., Collins S.R., Thompson N.J., Denic V., Bhamidipati A., Punna T., Ihmels J., Andrews B., Boone C., Greenblatt J.F., Weissman J.S., Krogan N.J.
    Cell 123:507-519(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN GET COMPLEX, SUBCELLULAR LOCATION.
  10. "The conserved ATPase Get3/Arr4 modulates the activity of membrane-associated proteins in Saccharomyces cerevisiae."
    Auld K.L., Hitchcock A.L., Doherty H.K., Frietze S., Huang L.S., Silver P.A.
    Genetics 174:215-227(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GET3.
  11. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  12. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "The GET complex mediates insertion of tail-anchored proteins into the ER membrane."
    Schuldiner M., Metz J., Schmid V., Denic V., Rakwalska M., Schmitt H.D., Schwappach B., Weissman J.S.
    Cell 134:634-645(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  14. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  15. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  16. "The mechanism of tail-anchored protein insertion into the ER membrane."
    Wang F., Whynot A., Tung M., Denic V.
    Mol. Cell 43:738-750(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 1-38 IN COMPLEX WITH GET3, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TOPOLOGY.
  17. "Structural basis for tail-anchored membrane protein biogenesis by the Get3-receptor complex."
    Stefer S., Reitz S., Wang F., Wild K., Pang Y.Y., Schwarz D., Bomke J., Hein C., Lohr F., Bernhard F., Denic V., Dotsch V., Sinning I.
    Science 333:758-762(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (4.6 ANGSTROMS) OF 2-35 IN COMPLEX WITH GET3, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TOPOLOGY.

Entry informationi

Entry nameiGET2_YEAST
AccessioniPrimary (citable) accession number: P40056
Secondary accession number(s): D3DLZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: May 16, 2006
Last modified: September 3, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

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