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P40056 (GET2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Golgi to ER traffic protein 2
Alternative name(s):
Guided entry of tail-anchored proteins 2
Hydroxyurea resistance protein 2
Required for meiotic nuclear division protein 7
Gene names
Name:GET2
Synonyms:HUR2, RMD7
Ordered Locus Names:YER083C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length285 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Together with GET1, acts as a membrane receptor for soluble GET3, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. The GET complex cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in DNA replication and DNA damage response and also in cell wall function. Ref.3 Ref.5 Ref.8 Ref.9 Ref.13 Ref.16 Ref.17

Subunit structure

Component of the Golgi to ER traffic (GET) complex, which is composed of GET1, GET2 and GET3. Ref.4 Ref.9 Ref.16 Ref.17

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein Ref.7 Ref.9 Ref.16 Ref.17.

Sequence similarities

Belongs to the GET2 family.

Sequence caution

The sequence AAB64638.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.15
Chain2 – 285284Golgi to ER traffic protein 2 HAMAP-Rule MF_03114
PRO_0000097362

Regions

Topological domain2 – 148147Cytoplasmic Potential
Transmembrane149 – 16921Helical; Potential
Topological domain170 – 19627Lumenal Potential
Transmembrane197 – 21620Helical; Potential
Topological domain217 – 26347Cytoplasmic Potential
Transmembrane264 – 28421Helical; Potential
Topological domain2851Lumenal Potential

Amino acid modifications

Modified residue21N-acetylserine Ref.15
Modified residue451Phosphoserine Ref.11
Glycosylation1731N-linked (GlcNAc...) Potential
Glycosylation1961N-linked (GlcNAc...) Potential

Secondary structure

..... 285
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P40056 [UniParc].

Last modified May 16, 2006. Version 2.
Checksum: 1667E7C879CFB6EA

FASTA28531,493
        10         20         30         40         50         60 
MSELTEAEKR RLLRERRQKK FSNGGASSRL NKITGQASSH LNAESPLDAP SAAKTTPPAS 

        70         80         90        100        110        120 
VHSATPDIKE DSNVAPQLDL LKQLAAMQGQ GTGKSTPQDS STPDLLSLLS SMNTGMPSAE 

       130        140        150        160        170        180 
GTPSFGQAAP AAPINQAALD YHDYLLNRLK AWTILVKWVF FLLPYLYLIT RPNSSVWPAY 

       190        200        210        220        230        240 
AFTQSAWFAP LRNPSNFTRI FATFEFLSIS IYYQLLKNVE HKSKIKNLQD TNKLVKLVSL 

       250        260        270        280 
VPEGVIPVAN LKGKLITLLQ YWDLLSMLIT DISFVLIVLG LLTYL 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Functional discovery via a compendium of expression profiles."
Hughes T.R., Marton M.J., Jones A.R., Roberts C.J., Stoughton R., Armour C.D., Bennett H.A., Coffey E., Dai H., He Y.D., Kidd M.J., King A.M., Meyer M.R., Slade D., Lum P.Y., Stepaniants S.B., Shoemaker D.D., Gachotte D. expand/collapse author list , Chakraburtty K., Simon J., Bard M., Friend S.H.
Cell 102:109-126(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry."
Ho Y., Gruhler A., Heilbut A., Bader G.D., Moore L., Adams S.-L., Millar A., Taylor P., Bennett K., Boutilier K., Yang L., Wolting C., Donaldson I., Schandorff S., Shewnarane J., Vo M., Taggart J., Goudreault M. expand/collapse author list , Muskat B., Alfarano C., Dewar D., Lin Z., Michalickova K., Willems A.R., Sassi H., Nielsen P.A., Rasmussen K.J., Andersen J.R., Johansen L.E., Hansen L.H., Jespersen H., Podtelejnikov A., Nielsen E., Crawford J., Poulsen V., Soerensen B.D., Matthiesen J., Hendrickson R.C., Gleeson F., Pawson T., Moran M.F., Durocher D., Mann M., Hogue C.W.V., Figeys D., Tyers M.
Nature 415:180-183(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN GET COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[5]"Large-scale functional genomic analysis of sporulation and meiosis in Saccharomyces cerevisiae."
Enyenihi A.H., Saunders W.S.
Genetics 163:47-54(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Sequencing and comparison of yeast species to identify genes and regulatory elements."
Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Novel functions of the phosphatidylinositol metabolic pathway discovered by a chemical genomics screen with wortmannin."
Zewail A., Xie M.W., Xing Y., Lin L., Zhang P.F., Zou W., Saxe J.P., Huang J.
Proc. Natl. Acad. Sci. U.S.A. 100:3345-3350(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Exploration of the function and organization of the yeast early secretory pathway through an epistatic miniarray profile."
Schuldiner M., Collins S.R., Thompson N.J., Denic V., Bhamidipati A., Punna T., Ihmels J., Andrews B., Boone C., Greenblatt J.F., Weissman J.S., Krogan N.J.
Cell 123:507-519(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN GET COMPLEX, SUBCELLULAR LOCATION.
[10]"The conserved ATPase Get3/Arr4 modulates the activity of membrane-associated proteins in Saccharomyces cerevisiae."
Auld K.L., Hitchcock A.L., Doherty H.K., Frietze S., Huang L.S., Silver P.A.
Genetics 174:215-227(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GET3.
[11]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[12]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"The GET complex mediates insertion of tail-anchored proteins into the ER membrane."
Schuldiner M., Metz J., Schmid V., Denic V., Rakwalska M., Schmitt H.D., Schwappach B., Weissman J.S.
Cell 134:634-645(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[14]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[15]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[16]"The mechanism of tail-anchored protein insertion into the ER membrane."
Wang F., Whynot A., Tung M., Denic V.
Mol. Cell 43:738-750(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 1-38 IN COMPLEX WITH GET3, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TOPOLOGY.
[17]"Structural basis for tail-anchored membrane protein biogenesis by the Get3-receptor complex."
Stefer S., Reitz S., Wang F., Wild K., Pang Y.Y., Schwarz D., Bomke J., Hein C., Lohr F., Bernhard F., Denic V., Dotsch V., Sinning I.
Science 333:758-762(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (4.6 ANGSTROMS) OF 2-35 IN COMPLEX WITH GET3, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TOPOLOGY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U18839 Genomic DNA. Translation: AAB64638.1. Different initiation.
BK006939 Genomic DNA. Translation: DAA07744.1.
PIRS50586.
RefSeqNP_011006.2. NM_001178974.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3SJDX-ray4.60D/E2-35[»]
3ZS9X-ray2.10C/D1-38[»]
ProteinModelPortalP40056.
SMRP40056. Positions 4-38.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36828. 696 interactions.
DIPDIP-4508N.
IntActP40056. 9 interactions.
MINTMINT-501836.
STRING4932.YER083C.

Protein family/group databases

TCDB3.A.21.1.1. the c-terminal tail-anchored membrane protein biogenesis/ insertion complex (tamp-b) family.

Proteomic databases

PaxDbP40056.
PeptideAtlasP40056.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYER083C; YER083C; YER083C.
GeneID856817.
KEGGsce:YER083C.

Organism-specific databases

CYGDYER083c.
SGDS000000885. GET2.

Phylogenomic databases

eggNOGNOG38846.
HOGENOMHOG000066100.
OMAIDEMFKK.
OrthoDBEOG708WB5.

Enzyme and pathway databases

BioCycYEAST:G3O-30253-MONOMER.

Gene expression databases

GenevestigatorP40056.

Family and domain databases

HAMAPMF_03114. Get2.
InterProIPR014802. GET2.
IPR028143. Get2/sif1.
[Graphical view]
PfamPF08690. GET2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP40056.
NextBio983091.

Entry information

Entry nameGET2_YEAST
AccessionPrimary (citable) accession number: P40056
Secondary accession number(s): D3DLZ0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: May 16, 2006
Last modified: April 16, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references