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P40056

- GET2_YEAST

UniProt

P40056 - GET2_YEAST

Protein

Golgi to ER traffic protein 2

Gene

GET2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 2 (16 May 2006)
      Previous versions | rss
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    Functioni

    Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Together with GET1, acts as a membrane receptor for soluble GET3, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. The GET complex cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in DNA replication and DNA damage response and also in cell wall function.7 PublicationsUniRule annotation

    GO - Molecular functioni

    1. protein anchor Source: SGD

    GO - Biological processi

    1. protein insertion into ER membrane Source: SGD
    2. retrograde vesicle-mediated transport, Golgi to ER Source: SGD

    Keywords - Biological processi

    ER-Golgi transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30253-MONOMER.

    Protein family/group databases

    TCDBi3.A.21.1.1. the c-terminal tail-anchored membrane protein biogenesis/ insertion complex (tamp-b) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Golgi to ER traffic protein 2UniRule annotation
    Alternative name(s):
    Guided entry of tail-anchored proteins 2
    Hydroxyurea resistance protein 2UniRule annotation
    Required for meiotic nuclear division protein 7UniRule annotation
    Gene namesi
    Name:GET2UniRule annotation
    Synonyms:HUR2UniRule annotation, RMD7UniRule annotation
    Ordered Locus Names:YER083C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYER083c.
    SGDiS000000885. GET2.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. GET complex Source: SGD
    3. Golgi membrane Source: UniProtKB-SubCell
    4. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 285284Golgi to ER traffic protein 2PRO_0000097362Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei45 – 451Phosphoserine1 Publication
    Glycosylationi173 – 1731N-linked (GlcNAc...)UniRule annotation
    Glycosylationi196 – 1961N-linked (GlcNAc...)UniRule annotation

    Keywords - PTMi

    Acetylation, Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP40056.
    PaxDbiP40056.
    PeptideAtlasiP40056.

    Expressioni

    Gene expression databases

    GenevestigatoriP40056.

    Interactioni

    Subunit structurei

    Component of the Golgi to ER traffic (GET) complex, which is composed of GET1, GET2 and GET3.4 PublicationsUniRule annotation

    Protein-protein interaction databases

    BioGridi36828. 697 interactions.
    DIPiDIP-4508N.
    IntActiP40056. 9 interactions.
    MINTiMINT-501836.
    STRINGi4932.YER083C.

    Structurei

    Secondary structure

    1
    285
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi6 – 2116
    Turni22 – 243
    Helixi25 – 339

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3SJDX-ray4.60D/E2-35[»]
    3ZS9X-ray2.10C/D1-38[»]
    ProteinModelPortaliP40056.
    SMRiP40056. Positions 4-38.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP40056.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 148147CytoplasmicUniRule annotationAdd
    BLAST
    Topological domaini170 – 19627LumenalUniRule annotationAdd
    BLAST
    Topological domaini217 – 26347CytoplasmicUniRule annotationAdd
    BLAST
    Topological domaini285 – 2851LumenalUniRule annotation

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei149 – 16921HelicalUniRule annotationAdd
    BLAST
    Transmembranei197 – 21620HelicalUniRule annotationAdd
    BLAST
    Transmembranei264 – 28421HelicalUniRule annotationAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GET2 family.UniRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG38846.
    HOGENOMiHOG000066100.
    OMAiIDEMFKK.
    OrthoDBiEOG708WB5.

    Family and domain databases

    HAMAPiMF_03114. Get2.
    InterProiIPR014802. GET2.
    IPR028143. Get2/sif1.
    [Graphical view]
    PfamiPF08690. GET2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P40056-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSELTEAEKR RLLRERRQKK FSNGGASSRL NKITGQASSH LNAESPLDAP    50
    SAAKTTPPAS VHSATPDIKE DSNVAPQLDL LKQLAAMQGQ GTGKSTPQDS 100
    STPDLLSLLS SMNTGMPSAE GTPSFGQAAP AAPINQAALD YHDYLLNRLK 150
    AWTILVKWVF FLLPYLYLIT RPNSSVWPAY AFTQSAWFAP LRNPSNFTRI 200
    FATFEFLSIS IYYQLLKNVE HKSKIKNLQD TNKLVKLVSL VPEGVIPVAN 250
    LKGKLITLLQ YWDLLSMLIT DISFVLIVLG LLTYL 285
    Length:285
    Mass (Da):31,493
    Last modified:May 16, 2006 - v2
    Checksum:i1667E7C879CFB6EA
    GO

    Sequence cautioni

    The sequence AAB64638.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18839 Genomic DNA. Translation: AAB64638.1. Different initiation.
    BK006939 Genomic DNA. Translation: DAA07744.1.
    PIRiS50586.
    RefSeqiNP_011006.2. NM_001178974.1.

    Genome annotation databases

    EnsemblFungiiYER083C; YER083C; YER083C.
    GeneIDi856817.
    KEGGisce:YER083C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18839 Genomic DNA. Translation: AAB64638.1 . Different initiation.
    BK006939 Genomic DNA. Translation: DAA07744.1 .
    PIRi S50586.
    RefSeqi NP_011006.2. NM_001178974.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3SJD X-ray 4.60 D/E 2-35 [» ]
    3ZS9 X-ray 2.10 C/D 1-38 [» ]
    ProteinModelPortali P40056.
    SMRi P40056. Positions 4-38.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36828. 697 interactions.
    DIPi DIP-4508N.
    IntActi P40056. 9 interactions.
    MINTi MINT-501836.
    STRINGi 4932.YER083C.

    Protein family/group databases

    TCDBi 3.A.21.1.1. the c-terminal tail-anchored membrane protein biogenesis/ insertion complex (tamp-b) family.

    Proteomic databases

    MaxQBi P40056.
    PaxDbi P40056.
    PeptideAtlasi P40056.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER083C ; YER083C ; YER083C .
    GeneIDi 856817.
    KEGGi sce:YER083C.

    Organism-specific databases

    CYGDi YER083c.
    SGDi S000000885. GET2.

    Phylogenomic databases

    eggNOGi NOG38846.
    HOGENOMi HOG000066100.
    OMAi IDEMFKK.
    OrthoDBi EOG708WB5.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30253-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P40056.
    NextBioi 983091.

    Gene expression databases

    Genevestigatori P40056.

    Family and domain databases

    HAMAPi MF_03114. Get2.
    InterProi IPR014802. GET2.
    IPR028143. Get2/sif1.
    [Graphical view ]
    Pfami PF08690. GET2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: FUNCTION.
    4. Cited for: IDENTIFICATION IN GET COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    5. "Large-scale functional genomic analysis of sporulation and meiosis in Saccharomyces cerevisiae."
      Enyenihi A.H., Saunders W.S.
      Genetics 163:47-54(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
      Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
      Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. "Novel functions of the phosphatidylinositol metabolic pathway discovered by a chemical genomics screen with wortmannin."
      Zewail A., Xie M.W., Xing Y., Lin L., Zhang P.F., Zou W., Saxe J.P., Huang J.
      Proc. Natl. Acad. Sci. U.S.A. 100:3345-3350(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Exploration of the function and organization of the yeast early secretory pathway through an epistatic miniarray profile."
      Schuldiner M., Collins S.R., Thompson N.J., Denic V., Bhamidipati A., Punna T., Ihmels J., Andrews B., Boone C., Greenblatt J.F., Weissman J.S., Krogan N.J.
      Cell 123:507-519(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN GET COMPLEX, SUBCELLULAR LOCATION.
    10. "The conserved ATPase Get3/Arr4 modulates the activity of membrane-associated proteins in Saccharomyces cerevisiae."
      Auld K.L., Hitchcock A.L., Doherty H.K., Frietze S., Huang L.S., Silver P.A.
      Genetics 174:215-227(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GET3.
    11. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    12. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "The GET complex mediates insertion of tail-anchored proteins into the ER membrane."
      Schuldiner M., Metz J., Schmid V., Denic V., Rakwalska M., Schmitt H.D., Schwappach B., Weissman J.S.
      Cell 134:634-645(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    14. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    15. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    16. "The mechanism of tail-anchored protein insertion into the ER membrane."
      Wang F., Whynot A., Tung M., Denic V.
      Mol. Cell 43:738-750(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 1-38 IN COMPLEX WITH GET3, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TOPOLOGY.
    17. "Structural basis for tail-anchored membrane protein biogenesis by the Get3-receptor complex."
      Stefer S., Reitz S., Wang F., Wild K., Pang Y.Y., Schwarz D., Bomke J., Hein C., Lohr F., Bernhard F., Denic V., Dotsch V., Sinning I.
      Science 333:758-762(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.6 ANGSTROMS) OF 2-35 IN COMPLEX WITH GET3, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TOPOLOGY.

    Entry informationi

    Entry nameiGET2_YEAST
    AccessioniPrimary (citable) accession number: P40056
    Secondary accession number(s): D3DLZ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: May 16, 2006
    Last modified: October 1, 2014
    This is version 123 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

    External Data

    Dasty 3