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Protein

Intermediate cleaving peptidase 55

Gene

ICP55

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Aminopeptidase which cleaves preprotein intermediates that carry destabilizing N-ter amino acid residues after the mitochondrial processing peptidase (MPP) cleavage site and is thus critical for stabilization of the mitochondrial proteome.2 Publications

Catalytic activityi

The enzyme cleaves the Pro(36)-Pro(37) bond of cysteine desulfurase (EC 2.8.1.7) removing three amino acid residues (Tyr-Ser-Pro) from the N-terminus after cleavage by mitochondrial processing peptidase.

Cofactori

Mn2+CuratedNote: Binds 2 manganese ions per subunit.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi327Manganese 2Sequence analysis1
Metal bindingi338Manganese 1Sequence analysis1
Metal bindingi338Manganese 2Sequence analysis1
Metal bindingi417Manganese 1Sequence analysis1
Metal bindingi444Manganese 1Sequence analysis1
Metal bindingi467Manganese 1Sequence analysis1
Metal bindingi467Manganese 2Sequence analysis1

GO - Molecular functioni

  • aminopeptidase activity Source: SGD
  • manganese ion binding Source: InterPro
  • metallopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  • protein processing Source: SGD
  • protein stabilization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:G3O-30249-MONOMER.
YEAST:G3O-30249-MONOMER.
BRENDAi3.4.11.26. 984.

Protein family/group databases

MEROPSiM24.026.

Names & Taxonomyi

Protein namesi
Recommended name:
Intermediate cleaving peptidase 55 (EC:3.4.11.26)
Alternative name(s):
Intermediate cleaving peptidase of 55 kDa
Gene namesi
Name:ICP55
Ordered Locus Names:YER078C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER078C.
SGDiS000000880. ICP55.

Subcellular locationi

GO - Cellular componenti

  • extrinsic component of mitochondrial inner membrane Source: SGD
  • mitochondrion Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001850981 – 511Intermediate cleaving peptidase 55Add BLAST511

Proteomic databases

MaxQBiP40051.
PRIDEiP40051.

Interactioni

Protein-protein interaction databases

BioGridi36823. 34 interactors.
DIPiDIP-4941N.
IntActiP40051. 1 interactor.
MINTiMINT-493000.

Structurei

3D structure databases

ProteinModelPortaliP40051.
SMRiP40051.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M24B family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000074909.
HOGENOMiHOG000008762.
InParanoidiP40051.
KOiK18573.
OMAiCILHYQQ.
OrthoDBiEOG092C1YL8.

Family and domain databases

Gene3Di3.40.350.10. 1 hit.
3.90.230.10. 1 hit.
InterProiIPR007865. Aminopep_P_N.
IPR029149. Creatin/AminoP/Spt16_NTD.
IPR028980. Creatinase/Aminopeptidase_P_N.
IPR000994. Pept_M24.
IPR001131. Peptidase_M24B_aminopep-P_CS.
[Graphical view]
PfamiPF05195. AMP_N. 1 hit.
PF00557. Peptidase_M24. 1 hit.
[Graphical view]
SMARTiSM01011. AMP_N. 1 hit.
[Graphical view]
SUPFAMiSSF53092. SSF53092. 1 hit.
SSF55920. SSF55920. 1 hit.
PROSITEiPS00491. PROLINE_PEPTIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40051-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLHRINPVRF SMQSCQRYFS KLVSPLEQHK SNTFTNRVRI PIEAGQPLHE
60 70 80 90 100
TRPFLIKSGE LTPGISALEY YERRIRLAET LPPKSCVILA GNDIQFASGA
110 120 130 140 150
VFYPFQQEND LFYLSGWNEP NSVMILEKPT DSLSDTIFHM LVPPKDAFAE
160 170 180 190 200
KWEGFRSGVY GVQEIFNADE SASINDLSKY LPKIINRNDF IYFDMLSTSN
210 220 230 240 250
PSSSNFKHIK SLLDGSGNSN RSLNSIANKT IKPISKRIAE FRKIKSPQEL
260 270 280 290 300
RIMRRAGQIS GRSFNQAFAK RFRNERTLDS FLHYKFISGG CDKDAYIPVV
310 320 330 340 350
ATGSNSLCIH YTRNDDVMFD DEMVLVDAAG SLGGYCADIS RTWPNSGKFT
360 370 380 390 400
DAQRDLYEAV LNVQRDCIKL CKASNNYSLH DIHEKSITLM KQELKNLGID
410 420 430 440 450
KVSGWNVEKL YPHYIGHNLG LDVHDVPKVS RYEPLKVGQV ITIEPGLYIP
460 470 480 490 500
NEESFPSYFR NVGIRIEDDI AIGEDTYTNL TVEAVKEIDD LENVMQNGLS
510
TKFEEDQVAP L
Length:511
Mass (Da):57,990
Last modified:February 1, 1995 - v1
Checksum:iB62FB0D0C53B5F2F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18839 Genomic DNA. Translation: AAB64633.1.
BK006939 Genomic DNA. Translation: DAA07738.1.
PIRiS50581.
RefSeqiNP_011001.1. NM_001178969.1.

Genome annotation databases

EnsemblFungiiYER078C; YER078C; YER078C.
GeneIDi856811.
KEGGisce:YER078C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18839 Genomic DNA. Translation: AAB64633.1.
BK006939 Genomic DNA. Translation: DAA07738.1.
PIRiS50581.
RefSeqiNP_011001.1. NM_001178969.1.

3D structure databases

ProteinModelPortaliP40051.
SMRiP40051.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36823. 34 interactors.
DIPiDIP-4941N.
IntActiP40051. 1 interactor.
MINTiMINT-493000.

Protein family/group databases

MEROPSiM24.026.

Proteomic databases

MaxQBiP40051.
PRIDEiP40051.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER078C; YER078C; YER078C.
GeneIDi856811.
KEGGisce:YER078C.

Organism-specific databases

EuPathDBiFungiDB:YER078C.
SGDiS000000880. ICP55.

Phylogenomic databases

GeneTreeiENSGT00550000074909.
HOGENOMiHOG000008762.
InParanoidiP40051.
KOiK18573.
OMAiCILHYQQ.
OrthoDBiEOG092C1YL8.

Enzyme and pathway databases

BioCyciMetaCyc:G3O-30249-MONOMER.
YEAST:G3O-30249-MONOMER.
BRENDAi3.4.11.26. 984.

Miscellaneous databases

PROiP40051.

Family and domain databases

Gene3Di3.40.350.10. 1 hit.
3.90.230.10. 1 hit.
InterProiIPR007865. Aminopep_P_N.
IPR029149. Creatin/AminoP/Spt16_NTD.
IPR028980. Creatinase/Aminopeptidase_P_N.
IPR000994. Pept_M24.
IPR001131. Peptidase_M24B_aminopep-P_CS.
[Graphical view]
PfamiPF05195. AMP_N. 1 hit.
PF00557. Peptidase_M24. 1 hit.
[Graphical view]
SMARTiSM01011. AMP_N. 1 hit.
[Graphical view]
SUPFAMiSSF53092. SSF53092. 1 hit.
SSF55920. SSF55920. 1 hit.
PROSITEiPS00491. PROLINE_PEPTIDASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiICP55_YEAST
AccessioniPrimary (citable) accession number: P40051
Secondary accession number(s): D3DLY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 30, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5080 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.