Skip Header

 
Contribute Send feedback

Reviewed, UniProtKB/Swiss-Prot P40047 (ALDH5_YEAST)

Last modified November 25, 2008. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Aldehyde dehydrogenase 5, mitochondrial
    EC=1.2.1.3
    EC=1.2.1.4
Gene names
Name: ALD5
Synonyms: ALD3, ALDH5
Ordered Locus Names: YER073W
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Hide | Top

Protein attributes

Sequence length520 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Function

Minor mitochondrial aldehyde dehydrogenase isoform. Plays a role in regulation or biosynthesis of electron transport chain components. Involved in the biosynthesis of acetate during anaerobic growth on glucose.

Catalytic activity

An aldehyde + NAD(+) + H(2)O = an acid + NADH.

An aldehyde + NADP(+) + H(2)O = an acid + NADPH.

Enzyme regulation

Induced by potassium ions.

Pathway

Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.

Subcellular location

Mitochondrion matrix.

Miscellaneous

Present with 23300 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the aldehyde dehydrogenase family.

Biophysicochemical properties

Kinetic parameters:

KM=0.64 mM for NADP (with acetaldehyde as cosubstrate)

KM=3.47 mM for NADP (with propionaldehyde as cosubstrate)

KM=6.43 mM for NAD (with propionaldehyde as cosubstrate)

KM=0.058 mM for acetaldehyde (with NADP as cosubstrate)

KM=0.39 mM for propionaldehyde (with NADP as cosubstrate)

KM=0.83 mM for propionaldehyde (with NAD as cosubstrate)

Vmax=1.1 µmol/min/mg enzyme with acetaldehyde and NADP as substrates

Vmax=0.45 µmol/min/mg enzyme with propionaldehyde and NADP as substrates

Vmax=0.011 µmol/min/mg enzyme with propionaldehyde and NAD as substrates

Hide | Top

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RAD52P067781EBI-5792,EBI-14719

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2323Mitochondrion Potential
Chain24 – 520497Aldehyde dehydrogenase 5, mitochondrial
PRO_0000007166

Regions

Nucleotide binding266 – 2716NAD By similarity

Sites

Active site2881Proton acceptor By similarity
Active site3221Nucleophile By similarity
Site1901Transition state stabilizer By similarity

Experimental info

Sequence conflict481I → T in AAB01220. Ref.1
Sequence conflict901A → L in AAB01220. Ref.1
Sequence conflict93 – 10412AFETK…IVEPE → LLKRSVYCRAG in AAB01220. Ref.1
Sequence conflict4111G → E in AAB01220. Ref.1

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
P40047-1 [UniParc].

Last modified April 29, 2008. Version 3.
Checksum: 316559CF2D9D2022

FASTA52056,621
        10         20         30         40         50         60 
MLSRTRAAAP NSRIFTRSLL RLYSQAPLRV PITLPNGFTY EQPTGLFING EFVASKQKKT 

        70         80         90        100        110        120 
FDVINPSNEE KITTVYKAME DDVDEAVAAA KKAFETKWSI VEPEVRAKAL FNLADLVEKH 

       130        140        150        160        170        180 
QETLAAIESM DNGKSLFCAR GDVALVSKYL RSCGGWADKI YGNVIDTGKN HFTYSIKEPL 

       190        200        210        220        230        240 
GVCGQIIPWN FPLLMWSWKI GPALATGNTV VLKPAETTPL SALFASQLCQ EAGIPAGVVN 

       250        260        270        280        290        300 
ILPGSGRVVG ERLSAHPDVK KIAFTGSTAT GRHIMKVAAD TVKKVTLELG GKSPNIVFAD 

       310        320        330        340        350        360 
ADLDKAVKNI AFGIFYNSGE VCCAGSRIYI QDTVYEEVLQ KLKDYTESLK VGDPFDEEVF 

       370        380        390        400        410        420 
QGAQTSDKQL HKILDYVDVA KSEGARLVTG GARHGSKGYF VKPTVFADVK GDMRIVKEEV 

       430        440        450        460        470        480 
FGPIVTVSKF STVDEVIAMA NDSQYGLAAG IHTNDINKAV DVSKRVKAGT VWINTYNNFH 

       490        500        510        520 
QNVPFGGFGQ SGIGREMGEA ALSNYTQTKS VRIAIDKPIR

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"Saccharomyces cerevisiae aldehyde dehydrogenases. Identification and expression."
Wang X., Bai Y., Ni L., Weiner H.
Adv. Exp. Med. Biol. 414:277-280(1997) [PubMed: 9059631] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed: 9169868] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]"Molecular cloning, characterization, and potential roles of cytosolic and mitochondrial aldehyde dehydrogenases in ethanol metabolism in Saccharomyces cerevisiae."
Wang X., Mann C.J., Bai Y., Ni L., Weiner H.
J. Bacteriol. 180:822-830(1998) [PubMed: 9473035] [Abstract]
Cited for: CHARACTERIZATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
[4]"Involvement of mitochondrial aldehyde dehydrogenase ALD5 in maintenance of the mitochondrial electron transport chain in Saccharomyces cerevisiae."
Kurita O., Nishida Y.
FEMS Microbiol. Lett. 181:281-287(1999) [PubMed: 10585550] [Abstract]
Cited for: FUNCTION.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed: 14562095] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"The proteome of Saccharomyces cerevisiae mitochondria."
Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C.
Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed: 14576278] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[8]"Functional analysis of the ALD gene family of Saccharomyces cerevisiae during anaerobic growth on glucose: the NADP+-dependent Ald6p and Ald5p isoforms play a major role in acetate formation."
Saint-Prix F., Boenquist L., Dequin S.
Microbiology 150:2209-2220(2004) [PubMed: 15256563] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases

U56605 Genomic DNA. Translation: AAB01220.1.
U18814 Genomic DNA. Translation: AAB64612.1.
PIRS50576.
RefSeqNP_010996.1.

3D structure databases

HSSPHSSP built from PDB template 1BI9 based on UniProtKB Q63639.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:3872N.
IntActP40047.

Proteomic databases

PeptideAtlasP40047.

Genome annotation databases

EnsemblYER073W. Saccharomyces cerevisiae. [Contig view]
GeneID856804.
GenomeReviewsGene locus YER073W in contig U00092_GR.
KEGGsce:YER073W.

Organism-specific databases

CYGDYER073w.
SGDS000000875. ALD5.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMP40047.

Enzyme and pathway databases

BioCycMetaCyc:MON-13665.

Gene expression databases

ArrayExpressP40047.
GermOnlineYER073W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR016160. Ald_DHase_CS.
IPR015590. Aldehyde_DHase.
[Graphical view]
PANTHERPTHR11699. Aldehyde_dehyd. 1 hit.
PROSITEPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

LinkHubP40047.
NextBio983056.

Hide | Top

Entry information

Entry nameALDH5_YEAST
AccessionPrimary (citable) accession number: P40047
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: April 29, 2008
Last modified: November 25, 2008
This is version 76 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Hide | Top

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents