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P40047

- ALDH5_YEAST

UniProt

P40047 - ALDH5_YEAST

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Protein

Aldehyde dehydrogenase 5, mitochondrial

Gene
ALD5, ALD3, ALDH5, YER073W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Minor mitochondrial aldehyde dehydrogenase isoform. Plays a role in regulation or biosynthesis of electron transport chain components. Involved in the biosynthesis of acetate during anaerobic growth on glucose.2 Publications

Catalytic activityi

An aldehyde + NAD(P)+ + H2O = a carboxylate + NAD(P)H.

Enzyme regulationi

Induced by potassium ions.

Kineticsi

  1. KM=0.64 mM for NADP (with acetaldehyde as cosubstrate)1 Publication
  2. KM=3.47 mM for NADP (with propionaldehyde as cosubstrate)
  3. KM=6.43 mM for NAD (with propionaldehyde as cosubstrate)
  4. KM=0.058 mM for acetaldehyde (with NADP as cosubstrate)
  5. KM=0.39 mM for propionaldehyde (with NADP as cosubstrate)
  6. KM=0.83 mM for propionaldehyde (with NAD as cosubstrate)

Vmax=1.1 µmol/min/mg enzyme with acetaldehyde and NADP as substrates

Vmax=0.45 µmol/min/mg enzyme with propionaldehyde and NADP as substrates

Vmax=0.011 µmol/min/mg enzyme with propionaldehyde and NAD as substrates

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei190 – 1901Transition state stabilizer By similarity
Active sitei288 – 2881Proton acceptor By similarity
Active sitei322 – 3221Nucleophile By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi266 – 2716NAD By similarity

GO - Molecular functioni

  1. aldehyde dehydrogenase (NAD) activity Source: SGD
  2. aldehyde dehydrogenase [NAD(P)+] activity Source: SGD

GO - Biological processi

  1. acetate biosynthetic process Source: SGD
  2. ethanol catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13665.
YEAST:MONOMER-13665.
YEAST:YER073W-MONOMER.
UniPathwayiUPA00780; UER00768.

Names & Taxonomyi

Protein namesi
Recommended name:
Aldehyde dehydrogenase 5, mitochondrial (EC:1.2.1.5)
Gene namesi
Name:ALD5
Synonyms:ALD3, ALDH5
Ordered Locus Names:YER073W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

SGDiS000000875. ALD5.

Subcellular locationi

Mitochondrion matrix 3 Publications

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2323Mitochondrion Reviewed predictionAdd
BLAST
Chaini24 – 520497Aldehyde dehydrogenase 5, mitochondrialPRO_0000007166Add
BLAST

Proteomic databases

MaxQBiP40047.
PaxDbiP40047.
PeptideAtlasiP40047.
PRIDEiP40047.

Expressioni

Gene expression databases

GenevestigatoriP40047.

Interactioni

Protein-protein interaction databases

BioGridi36816. 51 interactions.
DIPiDIP-3872N.
IntActiP40047. 9 interactions.
MINTiMINT-488437.
STRINGi4932.YER073W.

Structurei

3D structure databases

ProteinModelPortaliP40047.
SMRiP40047. Positions 9-517.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1012.
GeneTreeiENSGT00740000115528.
HOGENOMiHOG000271505.
KOiK00128.
OMAiHEGAEED.
OrthoDBiEOG7S226Z.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR015657. Aminobutyraldehyde_DH.
[Graphical view]
PANTHERiPTHR11699:SF46. PTHR11699:SF46. 1 hit.
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40047-1 [UniParc]FASTAAdd to Basket

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MLSRTRAAAP NSRIFTRSLL RLYSQAPLRV PITLPNGFTY EQPTGLFING    50
EFVASKQKKT FDVINPSNEE KITTVYKAME DDVDEAVAAA KKAFETKWSI 100
VEPEVRAKAL FNLADLVEKH QETLAAIESM DNGKSLFCAR GDVALVSKYL 150
RSCGGWADKI YGNVIDTGKN HFTYSIKEPL GVCGQIIPWN FPLLMWSWKI 200
GPALATGNTV VLKPAETTPL SALFASQLCQ EAGIPAGVVN ILPGSGRVVG 250
ERLSAHPDVK KIAFTGSTAT GRHIMKVAAD TVKKVTLELG GKSPNIVFAD 300
ADLDKAVKNI AFGIFYNSGE VCCAGSRIYI QDTVYEEVLQ KLKDYTESLK 350
VGDPFDEEVF QGAQTSDKQL HKILDYVDVA KSEGARLVTG GARHGSKGYF 400
VKPTVFADVK EDMRIVKEEV FGPIVTVSKF STVDEVIAMA NDSQYGLAAG 450
IHTNDINKAV DVSKRVKAGT VWINTYNNFH QNVPFGGFGQ SGIGREMGEA 500
ALSNYTQTKS VRIAIDKPIR 520
Length:520
Mass (Da):56,693
Last modified:July 27, 2011 - v4
Checksum:iDCD382A1157F800A
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti48 – 481I → T in AAB01220. 1 Publication
Sequence conflicti90 – 901A → L in AAB01220. 1 Publication
Sequence conflicti93 – 10412AFETK…IVEPE → LLKRSVYCRAG in AAB01220. 1 PublicationAdd
BLAST
Sequence conflicti411 – 4111E → G in AAB64612. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U56605 Genomic DNA. Translation: AAB01220.1.
U18814 Genomic DNA. Translation: AAB64612.1.
BK006939 Genomic DNA. Translation: DAA07732.2.
PIRiS50576.
RefSeqiNP_010996.2. NM_001178964.2.

Genome annotation databases

EnsemblFungiiYER073W; YER073W; YER073W.
GeneIDi856804.
KEGGisce:YER073W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U56605 Genomic DNA. Translation: AAB01220.1 .
U18814 Genomic DNA. Translation: AAB64612.1 .
BK006939 Genomic DNA. Translation: DAA07732.2 .
PIRi S50576.
RefSeqi NP_010996.2. NM_001178964.2.

3D structure databases

ProteinModelPortali P40047.
SMRi P40047. Positions 9-517.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36816. 51 interactions.
DIPi DIP-3872N.
IntActi P40047. 9 interactions.
MINTi MINT-488437.
STRINGi 4932.YER073W.

Proteomic databases

MaxQBi P40047.
PaxDbi P40047.
PeptideAtlasi P40047.
PRIDEi P40047.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER073W ; YER073W ; YER073W .
GeneIDi 856804.
KEGGi sce:YER073W.

Organism-specific databases

SGDi S000000875. ALD5.

Phylogenomic databases

eggNOGi COG1012.
GeneTreei ENSGT00740000115528.
HOGENOMi HOG000271505.
KOi K00128.
OMAi HEGAEED.
OrthoDBi EOG7S226Z.

Enzyme and pathway databases

UniPathwayi UPA00780 ; UER00768 .
BioCyci MetaCyc:MONOMER-13665.
YEAST:MONOMER-13665.
YEAST:YER073W-MONOMER.

Miscellaneous databases

NextBioi 983056.

Gene expression databases

Genevestigatori P40047.

Family and domain databases

Gene3Di 3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProi IPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR015657. Aminobutyraldehyde_DH.
[Graphical view ]
PANTHERi PTHR11699:SF46. PTHR11699:SF46. 1 hit.
Pfami PF00171. Aldedh. 1 hit.
[Graphical view ]
SUPFAMi SSF53720. SSF53720. 1 hit.
PROSITEi PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Saccharomyces cerevisiae aldehyde dehydrogenases. Identification and expression."
    Wang X., Bai Y., Ni L., Weiner H.
    Adv. Exp. Med. Biol. 414:277-280(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 411.
    Strain: ATCC 204508 / S288c.
  4. "Molecular cloning, characterization, and potential roles of cytosolic and mitochondrial aldehyde dehydrogenases in ethanol metabolism in Saccharomyces cerevisiae."
    Wang X., Mann C.J., Bai Y., Ni L., Weiner H.
    J. Bacteriol. 180:822-830(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
  5. "Involvement of mitochondrial aldehyde dehydrogenase ALD5 in maintenance of the mitochondrial electron transport chain in Saccharomyces cerevisiae."
    Kurita O., Nishida Y.
    FEMS Microbiol. Lett. 181:281-287(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  9. "Functional analysis of the ALD gene family of Saccharomyces cerevisiae during anaerobic growth on glucose: the NADP+-dependent Ald6p and Ald5p isoforms play a major role in acetate formation."
    Saint-Prix F., Boenquist L., Dequin S.
    Microbiology 150:2209-2220(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiALDH5_YEAST
AccessioniPrimary (citable) accession number: P40047
Secondary accession number(s): D3DLX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 27, 2011
Last modified: June 11, 2014
This is version 130 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 23300 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

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