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P40047

- ALDH5_YEAST

UniProt

P40047 - ALDH5_YEAST

Protein

Aldehyde dehydrogenase 5, mitochondrial

Gene

ALD5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 4 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Minor mitochondrial aldehyde dehydrogenase isoform. Plays a role in regulation or biosynthesis of electron transport chain components. Involved in the biosynthesis of acetate during anaerobic growth on glucose.2 Publications

    Catalytic activityi

    An aldehyde + NAD(P)+ + H2O = a carboxylate + NAD(P)H.

    Enzyme regulationi

    Induced by potassium ions.

    Kineticsi

    1. KM=0.64 mM for NADP (with acetaldehyde as cosubstrate)1 Publication
    2. KM=3.47 mM for NADP (with propionaldehyde as cosubstrate)1 Publication
    3. KM=6.43 mM for NAD (with propionaldehyde as cosubstrate)1 Publication
    4. KM=0.058 mM for acetaldehyde (with NADP as cosubstrate)1 Publication
    5. KM=0.39 mM for propionaldehyde (with NADP as cosubstrate)1 Publication
    6. KM=0.83 mM for propionaldehyde (with NAD as cosubstrate)1 Publication

    Vmax=1.1 µmol/min/mg enzyme with acetaldehyde and NADP as substrates1 Publication

    Vmax=0.45 µmol/min/mg enzyme with propionaldehyde and NADP as substrates1 Publication

    Vmax=0.011 µmol/min/mg enzyme with propionaldehyde and NAD as substrates1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei190 – 1901Transition state stabilizerBy similarity
    Active sitei288 – 2881Proton acceptorPROSITE-ProRule annotation
    Active sitei322 – 3221NucleophilePROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi266 – 2716NADBy similarity

    GO - Molecular functioni

    1. aldehyde dehydrogenase (NAD) activity Source: SGD
    2. aldehyde dehydrogenase [NAD(P)+] activity Source: SGD

    GO - Biological processi

    1. acetate biosynthetic process Source: SGD
    2. ethanol catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13665.
    YEAST:MONOMER-13665.
    YEAST:YER073W-MONOMER.
    UniPathwayiUPA00780; UER00768.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aldehyde dehydrogenase 5, mitochondrial (EC:1.2.1.5)
    Gene namesi
    Name:ALD5
    Synonyms:ALD3, ALDH5
    Ordered Locus Names:YER073W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    SGDiS000000875. ALD5.

    Subcellular locationi

    Mitochondrion matrix 3 Publications

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2323MitochondrionSequence AnalysisAdd
    BLAST
    Chaini24 – 520497Aldehyde dehydrogenase 5, mitochondrialPRO_0000007166Add
    BLAST

    Proteomic databases

    MaxQBiP40047.
    PaxDbiP40047.
    PeptideAtlasiP40047.
    PRIDEiP40047.

    Expressioni

    Gene expression databases

    GenevestigatoriP40047.

    Interactioni

    Protein-protein interaction databases

    BioGridi36816. 51 interactions.
    DIPiDIP-3872N.
    IntActiP40047. 9 interactions.
    MINTiMINT-488437.
    STRINGi4932.YER073W.

    Structurei

    3D structure databases

    ProteinModelPortaliP40047.
    SMRiP40047. Positions 9-517.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aldehyde dehydrogenase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1012.
    GeneTreeiENSGT00740000115528.
    HOGENOMiHOG000271505.
    KOiK00128.
    OMAiHEGAEED.
    OrthoDBiEOG7S226Z.

    Family and domain databases

    Gene3Di3.40.309.10. 1 hit.
    3.40.605.10. 1 hit.
    InterProiIPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016160. Ald_DH_CS_CYS.
    IPR029510. Ald_DH_CS_GLU.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    IPR015657. Aminobutyraldehyde_DH.
    [Graphical view]
    PANTHERiPTHR11699:SF46. PTHR11699:SF46. 1 hit.
    PfamiPF00171. Aldedh. 1 hit.
    [Graphical view]
    SUPFAMiSSF53720. SSF53720. 1 hit.
    PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
    PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P40047-1 [UniParc]FASTAAdd to Basket

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    MLSRTRAAAP NSRIFTRSLL RLYSQAPLRV PITLPNGFTY EQPTGLFING    50
    EFVASKQKKT FDVINPSNEE KITTVYKAME DDVDEAVAAA KKAFETKWSI 100
    VEPEVRAKAL FNLADLVEKH QETLAAIESM DNGKSLFCAR GDVALVSKYL 150
    RSCGGWADKI YGNVIDTGKN HFTYSIKEPL GVCGQIIPWN FPLLMWSWKI 200
    GPALATGNTV VLKPAETTPL SALFASQLCQ EAGIPAGVVN ILPGSGRVVG 250
    ERLSAHPDVK KIAFTGSTAT GRHIMKVAAD TVKKVTLELG GKSPNIVFAD 300
    ADLDKAVKNI AFGIFYNSGE VCCAGSRIYI QDTVYEEVLQ KLKDYTESLK 350
    VGDPFDEEVF QGAQTSDKQL HKILDYVDVA KSEGARLVTG GARHGSKGYF 400
    VKPTVFADVK EDMRIVKEEV FGPIVTVSKF STVDEVIAMA NDSQYGLAAG 450
    IHTNDINKAV DVSKRVKAGT VWINTYNNFH QNVPFGGFGQ SGIGREMGEA 500
    ALSNYTQTKS VRIAIDKPIR 520
    Length:520
    Mass (Da):56,693
    Last modified:July 27, 2011 - v4
    Checksum:iDCD382A1157F800A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti48 – 481I → T in AAB01220. (PubMed:9059631)Curated
    Sequence conflicti90 – 901A → L in AAB01220. (PubMed:9059631)Curated
    Sequence conflicti93 – 10412AFETK…IVEPE → LLKRSVYCRAG in AAB01220. (PubMed:9059631)CuratedAdd
    BLAST
    Sequence conflicti411 – 4111E → G in AAB64612. (PubMed:9169868)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U56605 Genomic DNA. Translation: AAB01220.1.
    U18814 Genomic DNA. Translation: AAB64612.1.
    BK006939 Genomic DNA. Translation: DAA07732.2.
    PIRiS50576.
    RefSeqiNP_010996.2. NM_001178964.2.

    Genome annotation databases

    EnsemblFungiiYER073W; YER073W; YER073W.
    GeneIDi856804.
    KEGGisce:YER073W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U56605 Genomic DNA. Translation: AAB01220.1 .
    U18814 Genomic DNA. Translation: AAB64612.1 .
    BK006939 Genomic DNA. Translation: DAA07732.2 .
    PIRi S50576.
    RefSeqi NP_010996.2. NM_001178964.2.

    3D structure databases

    ProteinModelPortali P40047.
    SMRi P40047. Positions 9-517.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36816. 51 interactions.
    DIPi DIP-3872N.
    IntActi P40047. 9 interactions.
    MINTi MINT-488437.
    STRINGi 4932.YER073W.

    Proteomic databases

    MaxQBi P40047.
    PaxDbi P40047.
    PeptideAtlasi P40047.
    PRIDEi P40047.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER073W ; YER073W ; YER073W .
    GeneIDi 856804.
    KEGGi sce:YER073W.

    Organism-specific databases

    SGDi S000000875. ALD5.

    Phylogenomic databases

    eggNOGi COG1012.
    GeneTreei ENSGT00740000115528.
    HOGENOMi HOG000271505.
    KOi K00128.
    OMAi HEGAEED.
    OrthoDBi EOG7S226Z.

    Enzyme and pathway databases

    UniPathwayi UPA00780 ; UER00768 .
    BioCyci MetaCyc:MONOMER-13665.
    YEAST:MONOMER-13665.
    YEAST:YER073W-MONOMER.

    Miscellaneous databases

    NextBioi 983056.

    Gene expression databases

    Genevestigatori P40047.

    Family and domain databases

    Gene3Di 3.40.309.10. 1 hit.
    3.40.605.10. 1 hit.
    InterProi IPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016160. Ald_DH_CS_CYS.
    IPR029510. Ald_DH_CS_GLU.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    IPR015657. Aminobutyraldehyde_DH.
    [Graphical view ]
    PANTHERi PTHR11699:SF46. PTHR11699:SF46. 1 hit.
    Pfami PF00171. Aldedh. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53720. SSF53720. 1 hit.
    PROSITEi PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
    PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Saccharomyces cerevisiae aldehyde dehydrogenases. Identification and expression."
      Wang X., Bai Y., Ni L., Weiner H.
      Adv. Exp. Med. Biol. 414:277-280(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 411.
      Strain: ATCC 204508 / S288c.
    4. "Molecular cloning, characterization, and potential roles of cytosolic and mitochondrial aldehyde dehydrogenases in ethanol metabolism in Saccharomyces cerevisiae."
      Wang X., Mann C.J., Bai Y., Ni L., Weiner H.
      J. Bacteriol. 180:822-830(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
    5. "Involvement of mitochondrial aldehyde dehydrogenase ALD5 in maintenance of the mitochondrial electron transport chain in Saccharomyces cerevisiae."
      Kurita O., Nishida Y.
      FEMS Microbiol. Lett. 181:281-287(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.
    9. "Functional analysis of the ALD gene family of Saccharomyces cerevisiae during anaerobic growth on glucose: the NADP+-dependent Ald6p and Ald5p isoforms play a major role in acetate formation."
      Saint-Prix F., Boenquist L., Dequin S.
      Microbiology 150:2209-2220(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiALDH5_YEAST
    AccessioniPrimary (citable) accession number: P40047
    Secondary accession number(s): D3DLX8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 131 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 23300 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3