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P40046

- VTC1_YEAST

UniProt

P40046 - VTC1_YEAST

Protein

Vacuolar transporter chaperone 1

Gene

VTC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Component of the vacuolar transporter chaperone (VTC) complex, which plays a role in vacuolar membrane fusion. Required for SEC18/NSF activity in SNARE priming, membrane binding of LMA1 and V0 trans-complex formation.3 Publications

    GO - Molecular functioni

    1. mRNA binding Source: SGD

    GO - Biological processi

    1. microautophagy Source: SGD
    2. polyphosphate metabolic process Source: SGD
    3. vacuolar transport Source: SGD
    4. vacuole fusion, non-autophagic Source: SGD

    Keywords - Molecular functioni

    Chaperone

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30244-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vacuolar transporter chaperone 1
    Alternative name(s):
    Negative regulator of CDC42 protein 1
    Phosphate metabolism protein 4
    Gene namesi
    Name:VTC1
    Synonyms:NRF1, PHM4
    Ordered Locus Names:YER072W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    SGDiS000000874. VTC1.

    Subcellular locationi

    Vacuole membrane 2 Publications; Multi-pass membrane protein 2 Publications

    GO - Cellular componenti

    1. endoplasmic reticulum Source: SGD
    2. integral component of membrane Source: UniProtKB-KW
    3. intrinsic component of vacuolar membrane Source: SGD
    4. vacuolar transporter chaperone complex Source: SGD

    Keywords - Cellular componenti

    Membrane, Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 129128Vacuolar transporter chaperone 1PRO_0000065934Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine2 Publications

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP40046.
    PaxDbiP40046.

    Expressioni

    Inductioni

    By low phosphate.1 Publication

    Gene expression databases

    GenevestigatoriP40046.

    Interactioni

    Subunit structurei

    The VTC complex is an integral membrane heterooligomer composed of VTC1, VTC2, VTC3 and VTC4. The complex interacts with the v-SNARE NYV1 and with the V0 subunit of V-ATPase VPH1.1 Publication

    Protein-protein interaction databases

    BioGridi36815. 92 interactions.
    IntActiP40046. 15 interactions.
    MINTiMINT-4484070.
    STRINGi4932.YER072W.

    Structurei

    3D structure databases

    ProteinModelPortaliP40046.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 3231Cytoplasmic1 PublicationAdd
    BLAST
    Topological domaini54 – 596Vacuolar1 Publication
    Topological domaini81 – 9818Cytoplasmic1 PublicationAdd
    BLAST
    Topological domaini120 – 12910Vacuolar1 Publication

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei33 – 5321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei60 – 8021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei99 – 11921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the VTC1 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5264.
    GeneTreeiENSGT00390000014774.
    HOGENOMiHOG000208407.
    OMAiLSWLHFT.
    OrthoDBiEOG7X6MBN.

    Family and domain databases

    InterProiIPR003807. DUF202.
    [Graphical view]
    PfamiPF02656. DUF202. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P40046-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSAPLLQRT PGKKIALPTR VEPKVFFANE RTFLSWLNFT VMLGGLGVGL    50
    LNFGDKIGRV SAGLFTFVAM GTMIYALVTY HWRAAAIRRR GSGPYDDRLG 100
    PTLLCFFLLV AVIINFILRL KYNDANTKL 129
    Length:129
    Mass (Da):14,371
    Last modified:January 23, 2007 - v3
    Checksum:i3D170007240E50B5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18813 Genomic DNA. Translation: AAB64608.1.
    BK006939 Genomic DNA. Translation: DAA07731.1.
    PIRiS50575.
    RefSeqiNP_010995.1. NM_001178963.1.

    Genome annotation databases

    EnsemblFungiiYER072W; YER072W; YER072W.
    GeneIDi856803.
    KEGGisce:YER072W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18813 Genomic DNA. Translation: AAB64608.1 .
    BK006939 Genomic DNA. Translation: DAA07731.1 .
    PIRi S50575.
    RefSeqi NP_010995.1. NM_001178963.1.

    3D structure databases

    ProteinModelPortali P40046.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36815. 92 interactions.
    IntActi P40046. 15 interactions.
    MINTi MINT-4484070.
    STRINGi 4932.YER072W.

    Proteomic databases

    MaxQBi P40046.
    PaxDbi P40046.

    Protocols and materials databases

    DNASUi 856803.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER072W ; YER072W ; YER072W .
    GeneIDi 856803.
    KEGGi sce:YER072W.

    Organism-specific databases

    SGDi S000000874. VTC1.

    Phylogenomic databases

    eggNOGi COG5264.
    GeneTreei ENSGT00390000014774.
    HOGENOMi HOG000208407.
    OMAi LSWLHFT.
    OrthoDBi EOG7X6MBN.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30244-MONOMER.

    Miscellaneous databases

    NextBioi 983053.

    Gene expression databases

    Genevestigatori P40046.

    Family and domain databases

    InterProi IPR003807. DUF202.
    [Graphical view ]
    Pfami PF02656. DUF202. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Bienvenut W.V., Peters C.
      Submitted (MAY-2005) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 2-9; 14-31 AND 91-98, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY.
    4. "A novel family of yeast chaperons involved in the distribution of V-ATPase and other membrane proteins."
      Cohen A., Perzov N., Nelson H., Nelson N.
      J. Biol. Chem. 274:26885-26893(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    5. "New components of a system for phosphate accumulation and polyphosphate metabolism in Saccharomyces cerevisiae revealed by genomic expression analysis."
      Ogawa N., DeRisi J.L., Brown P.O.
      Mol. Biol. Cell 11:4309-4321(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    6. "The Vtc proteins in vacuole fusion: coupling NSF activity to V(0) trans-complex formation."
      Mueller O., Bayer M.J., Peters C., Andersen J.S., Mann M., Mayer A.
      EMBO J. 21:259-269(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN VTC COMPLEX, SUBUNIT, INTERACTION WITH NYV1 AND VPH1.
    7. "Role of the Vtc proteins in V-ATPase stability and membrane trafficking."
      Mueller O., Neumann H., Bayer M.J., Mayer A.
      J. Cell Sci. 116:1107-1115(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TOPOLOGY, SUBCELLULAR LOCATION.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiVTC1_YEAST
    AccessioniPrimary (citable) accession number: P40046
    Secondary accession number(s): D3DLX7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 119 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 12074 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3