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Protein

Vacuolar transporter chaperone 1

Gene

VTC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the vacuolar transporter chaperone (VTC) complex, which plays a role in vacuolar membrane fusion. Required for SEC18/NSF activity in SNARE priming, membrane binding of LMA1 and V0 trans-complex formation.3 Publications

GO - Molecular functioni

  1. mRNA binding Source: SGD

GO - Biological processi

  1. microautophagy Source: SGD
  2. polyphosphate metabolic process Source: SGD
  3. vacuolar transport Source: SGD
  4. vacuole fusion, non-autophagic Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Enzyme and pathway databases

BioCyciYEAST:G3O-30244-MONOMER.

Protein family/group databases

TCDBi4.E.1.1.4. the vacuolar (acidocalcisome) polyphosphate polymerase (v-ppp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar transporter chaperone 1
Alternative name(s):
Negative regulator of CDC42 protein 1
Phosphate metabolism protein 4
Gene namesi
Name:VTC1
Synonyms:NRF1, PHM4
Ordered Locus Names:YER072W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

SGDiS000000874. VTC1.

Subcellular locationi

Vacuole membrane 2 Publications; Multi-pass membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 3231Cytoplasmic1 PublicationAdd
BLAST
Transmembranei33 – 5321HelicalSequence AnalysisAdd
BLAST
Topological domaini54 – 596Vacuolar1 Publication
Transmembranei60 – 8021HelicalSequence AnalysisAdd
BLAST
Topological domaini81 – 9818Cytoplasmic1 PublicationAdd
BLAST
Transmembranei99 – 11921HelicalSequence AnalysisAdd
BLAST
Topological domaini120 – 12910Vacuolar1 Publication

GO - Cellular componenti

  1. endoplasmic reticulum Source: SGD
  2. integral component of membrane Source: UniProtKB-KW
  3. intrinsic component of vacuolar membrane Source: SGD
  4. vacuolar transporter chaperone complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 129128Vacuolar transporter chaperone 1PRO_0000065934Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine2 Publications

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP40046.
PaxDbiP40046.

Expressioni

Inductioni

By low phosphate.1 Publication

Gene expression databases

GenevestigatoriP40046.

Interactioni

Subunit structurei

The VTC complex is an integral membrane heterooligomer composed of VTC1, VTC2, VTC3 and VTC4. The complex interacts with the v-SNARE NYV1 and with the V0 subunit of V-ATPase VPH1.1 Publication

Protein-protein interaction databases

BioGridi36815. 93 interactions.
IntActiP40046. 15 interactions.
MINTiMINT-4484070.
STRINGi4932.YER072W.

Structurei

3D structure databases

ProteinModelPortaliP40046.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the VTC1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5264.
GeneTreeiENSGT00390000014774.
HOGENOMiHOG000208407.
InParanoidiP40046.
OMAiLSWLHFT.
OrthoDBiEOG7X6MBN.

Family and domain databases

InterProiIPR003807. DUF202.
[Graphical view]
PfamiPF02656. DUF202. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40046-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSAPLLQRT PGKKIALPTR VEPKVFFANE RTFLSWLNFT VMLGGLGVGL
60 70 80 90 100
LNFGDKIGRV SAGLFTFVAM GTMIYALVTY HWRAAAIRRR GSGPYDDRLG
110 120
PTLLCFFLLV AVIINFILRL KYNDANTKL
Length:129
Mass (Da):14,371
Last modified:January 23, 2007 - v3
Checksum:i3D170007240E50B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18813 Genomic DNA. Translation: AAB64608.1.
BK006939 Genomic DNA. Translation: DAA07731.1.
PIRiS50575.
RefSeqiNP_010995.1. NM_001178963.1.

Genome annotation databases

EnsemblFungiiYER072W; YER072W; YER072W.
GeneIDi856803.
KEGGisce:YER072W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18813 Genomic DNA. Translation: AAB64608.1.
BK006939 Genomic DNA. Translation: DAA07731.1.
PIRiS50575.
RefSeqiNP_010995.1. NM_001178963.1.

3D structure databases

ProteinModelPortaliP40046.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36815. 93 interactions.
IntActiP40046. 15 interactions.
MINTiMINT-4484070.
STRINGi4932.YER072W.

Protein family/group databases

TCDBi4.E.1.1.4. the vacuolar (acidocalcisome) polyphosphate polymerase (v-ppp) family.

Proteomic databases

MaxQBiP40046.
PaxDbiP40046.

Protocols and materials databases

DNASUi856803.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER072W; YER072W; YER072W.
GeneIDi856803.
KEGGisce:YER072W.

Organism-specific databases

SGDiS000000874. VTC1.

Phylogenomic databases

eggNOGiCOG5264.
GeneTreeiENSGT00390000014774.
HOGENOMiHOG000208407.
InParanoidiP40046.
OMAiLSWLHFT.
OrthoDBiEOG7X6MBN.

Enzyme and pathway databases

BioCyciYEAST:G3O-30244-MONOMER.

Miscellaneous databases

NextBioi983053.

Gene expression databases

GenevestigatoriP40046.

Family and domain databases

InterProiIPR003807. DUF202.
[Graphical view]
PfamiPF02656. DUF202. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Bienvenut W.V., Peters C.
    Submitted (MAY-2005) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-9; 14-31 AND 91-98, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY.
  4. "A novel family of yeast chaperons involved in the distribution of V-ATPase and other membrane proteins."
    Cohen A., Perzov N., Nelson H., Nelson N.
    J. Biol. Chem. 274:26885-26893(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. "New components of a system for phosphate accumulation and polyphosphate metabolism in Saccharomyces cerevisiae revealed by genomic expression analysis."
    Ogawa N., DeRisi J.L., Brown P.O.
    Mol. Biol. Cell 11:4309-4321(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "The Vtc proteins in vacuole fusion: coupling NSF activity to V(0) trans-complex formation."
    Mueller O., Bayer M.J., Peters C., Andersen J.S., Mann M., Mayer A.
    EMBO J. 21:259-269(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN VTC COMPLEX, SUBUNIT, INTERACTION WITH NYV1 AND VPH1.
  7. "Role of the Vtc proteins in V-ATPase stability and membrane trafficking."
    Mueller O., Neumann H., Bayer M.J., Mayer A.
    J. Cell Sci. 116:1107-1115(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TOPOLOGY, SUBCELLULAR LOCATION.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiVTC1_YEAST
AccessioniPrimary (citable) accession number: P40046
Secondary accession number(s): D3DLX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: February 4, 2015
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 12074 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.