Vacuolar transporter chaperone 1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P40046 (VTC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 108. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Vacuolar transporter chaperone 1

Alternative name(s):
Negative regulator of CDC42 protein 1
Phosphate metabolism protein 4

Gene names

Name:	VTC1
Synonyms:	NRF1, PHM4
Ordered Locus Names:	YER072W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	129 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the vacuolar transporter chaperone (VTC) complex, which plays a role in vacuolar membrane fusion. Required for SEC18/NSF activity in SNARE priming, membrane binding of LMA1 and V₀ trans-complex formation. Ref.4 Ref.6 Ref.7
Subunit structure	The VTC complex is an integral membrane heterooligomer composed of VTC1, VTC2, VTC3 and VTC4. The complex interacts with the v-SNARE NYV1 and with the V₀ subunit of V-ATPase VPH1. Ref.6
Subcellular location	Vacuole membrane; Multi-pass membrane protein Ref.4 Ref.7.
Induction	By low phosphate. Ref.5
Miscellaneous	Present with 12074 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the VTC1 family.

Ontologies

Keywords
Cellular component	Membrane Vacuole
Domain	Transmembrane Transmembrane helix
Molecular function	Chaperone
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	microautophagy Inferred from direct assay PubMed 17079729. Source: SGD polyphosphate metabolic process Inferred from mutant phenotype Ref.5 PubMed 19390046. Source: SGD vacuolar transport Inferred from mutant phenotype PubMed 17079729. Source: SGD vacuole fusion, non-autophagic Inferred from mutant phenotype Ref.6. Source: SGD
Cellular_component	endoplasmic reticulum Inferred from direct assay PubMed 17079729. Source: SGD integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD intrinsic to vacuolar membrane Inferred from direct assay Ref.7. Source: SGD vacuolar transporter chaperone complex Inferred from physical interaction Ref.6. Source: SGD
Molecular_function	mRNA binding Inferred from direct assay PubMed 20844764. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.3

Chain

2 – 129

128

Vacuolar transporter chaperone 1

PRO_0000065934

Regions

Topological domain

2 – 32

Cytoplasmic Ref.7

Transmembrane

33 – 53

Helical; Potential

Topological domain

54 – 59

Vacuolar Ref.7

Transmembrane

60 – 80

Helical; Potential

Topological domain

81 – 98

Cytoplasmic Ref.7

Transmembrane

99 – 119

Helical; Potential

Topological domain

120 – 129

Vacuolar Ref.7

Amino acid modifications

Modified residue

N-acetylserine Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

P40046 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 3D170007240E50B5
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FASTA

129

14,371

        10         20         30         40         50         60 
MSSAPLLQRT PGKKIALPTR VEPKVFFANE RTFLSWLNFT VMLGGLGVGL LNFGDKIGRV 

        70         80         90        100        110        120 
SAGLFTFVAM GTMIYALVTY HWRAAAIRRR GSGPYDDRLG PTLLCFFLLV AVIINFILRL 


KYNDANTKL

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	Bienvenut W.V., Peters C. Submitted (MAY-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-9; 14-31 AND 91-98, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, MASS SPECTROMETRY.
[4]	"A novel family of yeast chaperons involved in the distribution of V-ATPase and other membrane proteins." Cohen A., Perzov N., Nelson H., Nelson N. J. Biol. Chem. 274:26885-26893(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]	"New components of a system for phosphate accumulation and polyphosphate metabolism in Saccharomyces cerevisiae revealed by genomic expression analysis." Ogawa N., DeRisi J.L., Brown P.O. Mol. Biol. Cell 11:4309-4321(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[6]	"The Vtc proteins in vacuole fusion: coupling NSF activity to V(0) trans-complex formation." Mueller O., Bayer M.J., Peters C., Andersen J.S., Mann M., Mayer A. EMBO J. 21:259-269(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN VTC COMPLEX, SUBUNIT, INTERACTION WITH NYV1 AND VPH1.
[7]	"Role of the Vtc proteins in V-ATPase stability and membrane trafficking." Mueller O., Neumann H., Bayer M.J., Mayer A. J. Cell Sci. 116:1107-1115(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TOPOLOGY, SUBCELLULAR LOCATION.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18813 Genomic DNA. Translation: AAB64608.1. BK006939 Genomic DNA. Translation: DAA07731.1.
PIR	S50575.
RefSeq	NP_010995.1. NM_001178963.1.
3D structure databases
ProteinModelPortal	P40046.
ModBase	Search...
Protein-protein interaction databases
IntAct	P40046. 15 interactions.
STRING	4932.YER072W.
Proteomic databases
PaxDb	P40046.
Protocols and materials databases
DNASU	856803.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER072W; YER072W; YER072W.
GeneID	856803.
KEGG	sce:YER072W.
Organism-specific databases
SGD	S000000874. VTC1.
Phylogenomic databases
eggNOG	COG5264.
HOGENOM	HOG000208407.
OMA	VTYHWRA.
OrthoDB	EOG4Z65Z5.
Gene expression databases
Genevestigator	P40046.
GermOnline	YER072W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003807. DUF202. IPR027177. VTC1. [Graphical view]
PANTHER	PTHR10783:SF1. PTHR10783:SF1. 1 hit.
Pfam	PF02656. DUF202. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	983053.

Entry information

Entry name

VTC1_YEAST

Accession

Primary (citable) accession number: P40046
Secondary accession number(s): D3DLX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 108 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents