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P40046 (VTC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vacuolar transporter chaperone 1
Alternative name(s):
Negative regulator of CDC42 protein 1
Phosphate metabolism protein 4
Gene names
Name:VTC1
Synonyms:NRF1, PHM4
Ordered Locus Names:YER072W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length129 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the vacuolar transporter chaperone (VTC) complex, which plays a role in vacuolar membrane fusion. Required for SEC18/NSF activity in SNARE priming, membrane binding of LMA1 and V0 trans-complex formation. Ref.4 Ref.6 Ref.7

Subunit structure

The VTC complex is an integral membrane heterooligomer composed of VTC1, VTC2, VTC3 and VTC4. The complex interacts with the v-SNARE NYV1 and with the V0 subunit of V-ATPase VPH1. Ref.6

Subcellular location

Vacuole membrane; Multi-pass membrane protein Ref.4 Ref.7.

Induction

By low phosphate. Ref.5

Miscellaneous

Present with 12074 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the VTC1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.3
Chain2 – 129128Vacuolar transporter chaperone 1
PRO_0000065934

Regions

Topological domain2 – 3231Cytoplasmic Ref.7
Transmembrane33 – 5321Helical; Potential
Topological domain54 – 596Vacuolar Ref.7
Transmembrane60 – 8021Helical; Potential
Topological domain81 – 9818Cytoplasmic Ref.7
Transmembrane99 – 11921Helical; Potential
Topological domain120 – 12910Vacuolar Ref.7

Amino acid modifications

Modified residue21N-acetylserine Ref.3 Ref.9

Sequences

Sequence LengthMass (Da)Tools
P40046 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 3D170007240E50B5

FASTA12914,371
        10         20         30         40         50         60 
MSSAPLLQRT PGKKIALPTR VEPKVFFANE RTFLSWLNFT VMLGGLGVGL LNFGDKIGRV 

        70         80         90        100        110        120 
SAGLFTFVAM GTMIYALVTY HWRAAAIRRR GSGPYDDRLG PTLLCFFLLV AVIINFILRL 


KYNDANTKL 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]Bienvenut W.V., Peters C.
Submitted (MAY-2005) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 2-9; 14-31 AND 91-98, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY.
[4]"A novel family of yeast chaperons involved in the distribution of V-ATPase and other membrane proteins."
Cohen A., Perzov N., Nelson H., Nelson N.
J. Biol. Chem. 274:26885-26893(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]"New components of a system for phosphate accumulation and polyphosphate metabolism in Saccharomyces cerevisiae revealed by genomic expression analysis."
Ogawa N., DeRisi J.L., Brown P.O.
Mol. Biol. Cell 11:4309-4321(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[6]"The Vtc proteins in vacuole fusion: coupling NSF activity to V(0) trans-complex formation."
Mueller O., Bayer M.J., Peters C., Andersen J.S., Mann M., Mayer A.
EMBO J. 21:259-269(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN VTC COMPLEX, SUBUNIT, INTERACTION WITH NYV1 AND VPH1.
[7]"Role of the Vtc proteins in V-ATPase stability and membrane trafficking."
Mueller O., Neumann H., Bayer M.J., Mayer A.
J. Cell Sci. 116:1107-1115(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TOPOLOGY, SUBCELLULAR LOCATION.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U18813 Genomic DNA. Translation: AAB64608.1.
BK006939 Genomic DNA. Translation: DAA07731.1.
PIRS50575.
RefSeqNP_010995.1. NM_001178963.1.

3D structure databases

ProteinModelPortalP40046.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36815. 92 interactions.
IntActP40046. 15 interactions.
MINTMINT-4484070.
STRING4932.YER072W.

Proteomic databases

MaxQBP40046.
PaxDbP40046.

Protocols and materials databases

DNASU856803.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYER072W; YER072W; YER072W.
GeneID856803.
KEGGsce:YER072W.

Organism-specific databases

SGDS000000874. VTC1.

Phylogenomic databases

eggNOGCOG5264.
GeneTreeENSGT00390000014774.
HOGENOMHOG000208407.
OMALSWLHFT.
OrthoDBEOG7X6MBN.

Enzyme and pathway databases

BioCycYEAST:G3O-30244-MONOMER.

Gene expression databases

GenevestigatorP40046.

Family and domain databases

InterProIPR003807. DUF202.
[Graphical view]
PfamPF02656. DUF202. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio983053.

Entry information

Entry nameVTC1_YEAST
AccessionPrimary (citable) accession number: P40046
Secondary accession number(s): D3DLX7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families