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Protein

Protein THO1

Gene

THO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Unknown; suppressor of the transcriptional defect of HPR1 by overexpression.

GO - Molecular functioni

  1. chromatin binding Source: SGD
  2. double-stranded DNA binding Source: SGD
  3. RNA binding Source: SGD

GO - Biological processi

  1. poly(A)+ mRNA export from nucleus Source: SGD
  2. ribonucleoprotein complex assembly Source: SGD
  3. transcription, DNA-templated Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-30238-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein THO1
Gene namesi
Name:THO1
Ordered Locus Names:YER063W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome V

Organism-specific databases

CYGDiYER063w.
SGDiS000000865. THO1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 218218Protein THO1PRO_0000072519Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei22 – 221Phosphoserine1 Publication
Modified residuei58 – 581Phosphoserine1 Publication
Modified residuei68 – 681Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40040.
PaxDbiP40040.
PeptideAtlasiP40040.

Expressioni

Gene expression databases

GenevestigatoriP40040.

Interactioni

Protein-protein interaction databases

BioGridi36805. 26 interactions.
DIPiDIP-3983N.
IntActiP40040. 2 interactions.
MINTiMINT-494243.
STRINGi4932.YER063W.

Structurei

Secondary structure

1
218
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 63Combined sources
Helixi9 – 1810Combined sources
Helixi27 – 4014Combined sources
Helixi122 – 14322Combined sources
Helixi147 – 16216Combined sources
Helixi170 – 1756Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1H1JNMR-S2-50[»]
2WQGNMR-A2-50[»]
4UZWNMR-A2-50[»]
4UZXNMR-A119-183[»]
ProteinModelPortaliP40040.
SMRiP40040. Positions 2-43.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40040.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 3835SAPPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 SAP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG48052.
HOGENOMiHOG000000840.
InParanoidiP40040.
KOiK18732.
OMAiVRVEKFG.
OrthoDBiEOG76HQD6.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
InterProiIPR003034. SAP_dom.
[Graphical view]
PfamiPF02037. SAP. 1 hit.
[Graphical view]
SMARTiSM00513. SAP. 1 hit.
[Graphical view]
PROSITEiPS50800. SAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40040-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADYSSLTVV QLKDLLTKRN LSVGGLKNEL VQRLIKDDEE SKGESEVSPQ
60 70 80 90 100
EQNQEQGSEP AAIEEPASQN ITEKKEVSSE PKETNEPKEE NKDVQKPSDG
110 120 130 140 150
PSATASENEQ AAASTAAPAL SPEEIKAKAL DLLNKKLHRA NKFGQDQADI
160 170 180 190 200
DSLQRQINRV EKFGVDLNSK LAEELGLVSR KNEPESGNNG KFKNRNKNAN
210
NRSRVSKNRR GNRSGYRR
Length:218
Mass (Da):24,138
Last modified:February 1, 1995 - v1
Checksum:i39CB897BA9FBA94A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18813 Genomic DNA. Translation: AAB64599.1.
AY557779 Genomic DNA. Translation: AAS56105.1.
BK006939 Genomic DNA. Translation: DAA07722.1.
PIRiS50566.
RefSeqiNP_010985.1. NM_001178954.1.

Genome annotation databases

EnsemblFungiiYER063W; YER063W; YER063W.
GeneIDi856792.
KEGGisce:YER063W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18813 Genomic DNA. Translation: AAB64599.1.
AY557779 Genomic DNA. Translation: AAS56105.1.
BK006939 Genomic DNA. Translation: DAA07722.1.
PIRiS50566.
RefSeqiNP_010985.1. NM_001178954.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1H1JNMR-S2-50[»]
2WQGNMR-A2-50[»]
4UZWNMR-A2-50[»]
4UZXNMR-A119-183[»]
ProteinModelPortaliP40040.
SMRiP40040. Positions 2-43.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36805. 26 interactions.
DIPiDIP-3983N.
IntActiP40040. 2 interactions.
MINTiMINT-494243.
STRINGi4932.YER063W.

Proteomic databases

MaxQBiP40040.
PaxDbiP40040.
PeptideAtlasiP40040.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER063W; YER063W; YER063W.
GeneIDi856792.
KEGGisce:YER063W.

Organism-specific databases

CYGDiYER063w.
SGDiS000000865. THO1.

Phylogenomic databases

eggNOGiNOG48052.
HOGENOMiHOG000000840.
InParanoidiP40040.
KOiK18732.
OMAiVRVEKFG.
OrthoDBiEOG76HQD6.

Enzyme and pathway databases

BioCyciYEAST:G3O-30238-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP40040.
NextBioi983024.
PROiP40040.

Gene expression databases

GenevestigatoriP40040.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
InterProiIPR003034. SAP_dom.
[Graphical view]
PfamiPF02037. SAP. 1 hit.
[Graphical view]
SMARTiSM00513. SAP. 1 hit.
[Graphical view]
PROSITEiPS50800. SAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "A novel yeast gene, THO2, is involved in RNA pol II transcription and provides new evidence for transcriptional elongation-associated recombination."
    Piruat J.I., Aguilera A.
    EMBO J. 17:4859-4872(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-58 AND SER-68, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTHO1_YEAST
AccessioniPrimary (citable) accession number: P40040
Secondary accession number(s): D3DLW8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 1, 2015
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6580 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.