SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P40038

- PCL6_YEAST

UniProt

P40038 - PCL6_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
PHO85 cyclin-6
Gene
PCL6, YER059W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Cyclin partner of the cyclin-dependent kinase (CDK) PHO85. Together with cyclin PCL7, controls glycogen phosphorylase and glycogen synthase activities in response to nutrient availablility. The PCL6-PHO85 cyclin-CDK holoenzyme has GLC8 kinase activity and phosphorylates and inactivates the phosphatase PP1-2 inhibitor GLC8, causing activation of PP1-2, which then dephosphorylates and activates glycogen phosphorylase. PCL6-PHO85 also phosphorylates YJL084C.5 Publications

GO - Molecular functioni

  1. cyclin-dependent protein serine/threonine kinase regulator activity Source: SGD
  2. protein binding Source: IntAct

GO - Biological processi

  1. glycogen metabolic process Source: UniProtKB-KW
  2. regulation of cyclin-dependent protein serine/threonine kinase activity Source: InterPro
  3. regulation of glycogen biosynthetic process Source: SGD
  4. regulation of glycogen catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Cyclin

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Enzyme and pathway databases

BioCyciYEAST:G3O-30236-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
PHO85 cyclin-6
Gene namesi
Name:PCL6
Ordered Locus Names:YER059W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER059w.
SGDiS000000861. PCL6.

Subcellular locationi

Cytoplasm. Nucleus 1 Publication

GO - Cellular componenti

  1. cyclin-dependent protein kinase holoenzyme complex Source: SGD
  2. cytoplasm Source: UniProtKB-SubCell
  3. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 420420PHO85 cyclin-6
PRO_0000202631Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei61 – 611Phosphoserine1 Publication
Modified residuei281 – 2811Phosphoserine1 Publication
Modified residuei312 – 3121Phosphoserine2 Publications
Modified residuei317 – 3171Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40038.
PaxDbiP40038.

Expressioni

Gene expression databases

GenevestigatoriP40038.

Interactioni

Subunit structurei

Forms a cyclin-CDK complex with PHO85. Interacts with the substrate protein YJL084C. Interacts with elongin-C, which stabilizes PCL6. Interacts with the CDK inhibitor (CKI) PHO81.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PHO85P171577EBI-22555,EBI-13327

Protein-protein interaction databases

BioGridi36800. 36 interactions.
DIPiDIP-1496N.
IntActiP40038. 20 interactions.
MINTiMINT-394507.
STRINGi4932.YER059W.

Structurei

3D structure databases

ProteinModelPortaliP40038.
SMRiP40038. Positions 332-395.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG256941.
GeneTreeiENSGT00530000066532.
HOGENOMiHOG000074708.
OrthoDBiEOG7PP5HV.

Family and domain databases

InterProiIPR013922. Cyclin_PHO80-like.
[Graphical view]
PfamiPF08613. Cyclin. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40038-1 [UniParc]FASTAAdd to Basket

« Hide

MSIKGDSPSS TNASSSPKST YSIQSDDKAN LGSGNVDIRT DNSQQDSNNR    50
RDIVVVTRVA SEETLESQSS TSSMGIRPES SFNYEDASNQ ARVEMNNRVH 100
GSNMNTINKY YPVRFPKNNE RQLSDTNNLN EKVQGTHTVQ SSTQEDKILD 150
GDTSNSQVTP SLNIAEFPTD KLLKMLTALL TKIIKSNDRT AATNPSLTQE 200
IENGRCLALS DNEKKYLSPV LGFRGKHVPQ IGLDQYFQRI QKYCPTTNDV 250
FLSLLVYFDR ISKRCNSVTT TPKTNTAKHE SPSNESSLDK ANRGADKMSA 300
CNSNENNEND DSDDENTGVQ RDSRAHPQMF VMDSHNIHRL IIAGITVSTK 350
FLSDFFYSNS RYSRVGGISL QELNHLELQF LVLCDFELLI SVNELQRYAD 400
LLYRFWNNAK AQSQALVTGM 420
Length:420
Mass (Da):47,007
Last modified:February 1, 1995 - v1
Checksum:iBD8A0C7A501C94E5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18813 Genomic DNA. Translation: AAB64595.1.
BK006939 Genomic DNA. Translation: DAA07717.1.
PIRiS50562.
RefSeqiNP_010980.1. NM_001178950.1.

Genome annotation databases

EnsemblFungiiYER059W; YER059W; YER059W.
GeneIDi856787.
KEGGisce:YER059W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18813 Genomic DNA. Translation: AAB64595.1 .
BK006939 Genomic DNA. Translation: DAA07717.1 .
PIRi S50562.
RefSeqi NP_010980.1. NM_001178950.1.

3D structure databases

ProteinModelPortali P40038.
SMRi P40038. Positions 332-395.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36800. 36 interactions.
DIPi DIP-1496N.
IntActi P40038. 20 interactions.
MINTi MINT-394507.
STRINGi 4932.YER059W.

Proteomic databases

MaxQBi P40038.
PaxDbi P40038.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER059W ; YER059W ; YER059W .
GeneIDi 856787.
KEGGi sce:YER059W.

Organism-specific databases

CYGDi YER059w.
SGDi S000000861. PCL6.

Phylogenomic databases

eggNOGi NOG256941.
GeneTreei ENSGT00530000066532.
HOGENOMi HOG000074708.
OrthoDBi EOG7PP5HV.

Enzyme and pathway databases

BioCyci YEAST:G3O-30236-MONOMER.

Miscellaneous databases

NextBioi 983009.

Gene expression databases

Genevestigatori P40038.

Family and domain databases

InterProi IPR013922. Cyclin_PHO80-like.
[Graphical view ]
Pfami PF08613. Cyclin. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "A family of cyclin-like proteins that interact with the Pho85 cyclin-dependent kinase."
    Measday V., Moore L., Retnakaran R., Lee J., Donoviel M., Neiman A.M., Andrews B.J.
    Mol. Cell. Biol. 17:1212-1223(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PHO85.
  4. Cited for: INTERACTION WITH ELC1.
  5. Cited for: ENZYME REGULATION, INTERACTION WITH PHO81 AND PHO85.
  6. "The yeast cyclins Pcl6p and Pcl7p are involved in the control of glycogen storage by the cyclin-dependent protein kinase Pho85p."
    Wang Z., Wilson W.A., Fujino M.A., Roach P.J.
    FEBS Lett. 506:277-280(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Analysis of phosphorylation of YJL084c, a yeast protein."
    Shi X.Z., Ao S.Z.
    Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 34:433-438(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH YJL084C, PHOSPHORYLATION OF YJL084C.
  8. "Binding to Elongin C inhibits degradation of interacting proteins in yeast."
    Hyman L.E., Kwon E., Ghosh S., McGee J., Chachulska A.M., Jackson T., Baricos W.H.
    J. Biol. Chem. 277:15586-15591(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Pho85 phosphorylates the Glc7 protein phosphatase regulator Glc8 in vivo."
    Tan Y.S.H., Morcos P.A., Cannon J.F.
    J. Biol. Chem. 278:147-153(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION OF GLC8.
  10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  12. "Regulation of yeast glycogen phosphorylase by the cyclin-dependent protein kinase Pho85p."
    Wilson W.A., Wang Z., Roach P.J.
    Biochem. Biophys. Res. Commun. 329:161-167(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-61; SER-281 AND SER-312, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-312 AND THR-317, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPCL6_YEAST
AccessioniPrimary (citable) accession number: P40038
Secondary accession number(s): D3DLW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1240 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi