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P40038 (PCL6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
PHO85 cyclin-6
Gene names
Name:PCL6
Ordered Locus Names:YER059W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length420 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cyclin partner of the cyclin-dependent kinase (CDK) PHO85. Together with cyclin PCL7, controls glycogen phosphorylase and glycogen synthase activities in response to nutrient availablility. The PCL6-PHO85 cyclin-CDK holoenzyme has GLC8 kinase activity and phosphorylates and inactivates the phosphatase PP1-2 inhibitor GLC8, causing activation of PP1-2, which then dephosphorylates and activates glycogen phosphorylase. PCL6-PHO85 also phosphorylates YJL084C. Ref.6 Ref.7 Ref.8 Ref.9 Ref.12

Subunit structure

Forms a cyclin-CDK complex with PHO85. Interacts with the substrate protein YJL084C. Interacts with elongin-C, which stabilizes PCL6. Interacts with the CDK inhibitor (CKI) PHO81. Ref.3 Ref.4 Ref.5 Ref.7

Subcellular location

Cytoplasm. Nucleus Ref.10.

Miscellaneous

Present with 1240 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the cyclin family. PHO80 subfamily.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PHO85P171577EBI-22555,EBI-13327

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 420420PHO85 cyclin-6
PRO_0000202631

Amino acid modifications

Modified residue611Phosphoserine Ref.13
Modified residue2811Phosphoserine Ref.13
Modified residue3121Phosphoserine Ref.13 Ref.14
Modified residue3171Phosphothreonine Ref.14

Sequences

Sequence LengthMass (Da)Tools
P40038 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: BD8A0C7A501C94E5

FASTA42047,007
        10         20         30         40         50         60 
MSIKGDSPSS TNASSSPKST YSIQSDDKAN LGSGNVDIRT DNSQQDSNNR RDIVVVTRVA 

        70         80         90        100        110        120 
SEETLESQSS TSSMGIRPES SFNYEDASNQ ARVEMNNRVH GSNMNTINKY YPVRFPKNNE 

       130        140        150        160        170        180 
RQLSDTNNLN EKVQGTHTVQ SSTQEDKILD GDTSNSQVTP SLNIAEFPTD KLLKMLTALL 

       190        200        210        220        230        240 
TKIIKSNDRT AATNPSLTQE IENGRCLALS DNEKKYLSPV LGFRGKHVPQ IGLDQYFQRI 

       250        260        270        280        290        300 
QKYCPTTNDV FLSLLVYFDR ISKRCNSVTT TPKTNTAKHE SPSNESSLDK ANRGADKMSA 

       310        320        330        340        350        360 
CNSNENNEND DSDDENTGVQ RDSRAHPQMF VMDSHNIHRL IIAGITVSTK FLSDFFYSNS 

       370        380        390        400        410        420 
RYSRVGGISL QELNHLELQF LVLCDFELLI SVNELQRYAD LLYRFWNNAK AQSQALVTGM 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"A family of cyclin-like proteins that interact with the Pho85 cyclin-dependent kinase."
Measday V., Moore L., Retnakaran R., Lee J., Donoviel M., Neiman A.M., Andrews B.J.
Mol. Cell. Biol. 17:1212-1223(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PHO85.
[4]"Novel roles for elongin C in yeast."
Jackson T., Kwon E., Chachulska A.M., Hyman L.E.
Biochim. Biophys. Acta 1491:161-176(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ELC1.
[5]"Regulation of the Pcl7-Pho85 cyclin-cdk complex by Pho81."
Lee M., O'Regan S., Moreau J.-L., Johnson A.L., Johnston L.H., Goding C.R.
Mol. Microbiol. 38:411-422(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION, INTERACTION WITH PHO81 AND PHO85.
[6]"The yeast cyclins Pcl6p and Pcl7p are involved in the control of glycogen storage by the cyclin-dependent protein kinase Pho85p."
Wang Z., Wilson W.A., Fujino M.A., Roach P.J.
FEBS Lett. 506:277-280(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Analysis of phosphorylation of YJL084c, a yeast protein."
Shi X.Z., Ao S.Z.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 34:433-438(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH YJL084C, PHOSPHORYLATION OF YJL084C.
[8]"Binding to Elongin C inhibits degradation of interacting proteins in yeast."
Hyman L.E., Kwon E., Ghosh S., McGee J., Chachulska A.M., Jackson T., Baricos W.H.
J. Biol. Chem. 277:15586-15591(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Pho85 phosphorylates the Glc7 protein phosphatase regulator Glc8 in vivo."
Tan Y.S.H., Morcos P.A., Cannon J.F.
J. Biol. Chem. 278:147-153(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PHOSPHORYLATION OF GLC8.
[10]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[11]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[12]"Regulation of yeast glycogen phosphorylase by the cyclin-dependent protein kinase Pho85p."
Wilson W.A., Wang Z., Roach P.J.
Biochem. Biophys. Res. Commun. 329:161-167(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[13]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-61; SER-281 AND SER-312, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-312 AND THR-317, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U18813 Genomic DNA. Translation: AAB64595.1.
BK006939 Genomic DNA. Translation: DAA07717.1.
PIRS50562.
RefSeqNP_010980.1. NM_001178950.1.

3D structure databases

ProteinModelPortalP40038.
SMRP40038. Positions 332-395.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36800. 36 interactions.
DIPDIP-1496N.
IntActP40038. 20 interactions.
MINTMINT-394507.
STRING4932.YER059W.

Proteomic databases

PaxDbP40038.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYER059W; YER059W; YER059W.
GeneID856787.
KEGGsce:YER059W.

Organism-specific databases

CYGDYER059w.
SGDS000000861. PCL6.

Phylogenomic databases

eggNOGNOG256941.
GeneTreeENSGT00530000066532.
HOGENOMHOG000074708.
OrthoDBEOG7PP5HV.

Enzyme and pathway databases

BioCycYEAST:G3O-30236-MONOMER.

Gene expression databases

GenevestigatorP40038.

Family and domain databases

InterProIPR013922. Cyclin_PHO80-like.
[Graphical view]
PfamPF08613. Cyclin. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio983009.

Entry information

Entry namePCL6_YEAST
AccessionPrimary (citable) accession number: P40038
Secondary accession number(s): D3DLW3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 19, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families