PHO85 cyclin-6 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P40038 (PCL6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 87. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
PHO85 cyclin-6

Gene names

Name:	PCL6
Ordered Locus Names:	YER059W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cyclin partner of the cyclin-dependent kinase (CDK) PHO85. Together with cyclin PCL7, controls glycogen phosphorylase and glycogen synthase activities in response to nutrient availablility. The PCL6-PHO85 cyclin-CDK holoenzyme has GLC8 kinase activity and phosphorylates and inactivates the phosphatase PP1-2 inhibitor GLC8, causing activation of PP1-2, which then dephosphorylates and activates glycogen phosphorylase. PCL6-PHO85 also phosphorylates YJL084C. Ref.6 Ref.7 Ref.8 Ref.9 Ref.12
Subunit structure	Forms a cyclin-CDK complex with PHO85. Interacts with the substrate protein YJL084C. Interacts with elongin-C, which stabilizes PCL6. Interacts with the CDK inhibitor (CKI) PHO81. Ref.3 Ref.4 Ref.5 Ref.7
Subcellular location	Cytoplasm. Nucleus Ref.10.
Miscellaneous	Present with 1240 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the cyclin family. PHO80 subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen metabolism
Cellular component	Cytoplasm Nucleus
Molecular function	Cyclin
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycogen metabolic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: InterPro regulation of glycogen biosynthetic process Inferred from genetic interaction Ref.6. Source: SGD regulation of glycogen catabolic process Inferred from genetic interaction Ref.6. Source: SGD
Cellular_component	cyclin-dependent protein kinase holoenzyme complex Traceable author statement Ref.6. Source: SGD cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cyclin-dependent protein serine/threonine kinase regulator activity Traceable author statement Ref.6. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PHO85	P17157	8	EBI-22555,EBI-13327

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 420

420

PHO85 cyclin-6

PRO_0000202631

Amino acid modifications

Modified residue

Phosphotyrosine Ref.14

Modified residue

Phosphoserine Ref.14

Modified residue

Phosphoserine Ref.13 Ref.14

Modified residue

Phosphoserine Ref.14

Modified residue

281

Phosphoserine Ref.13 Ref.14

Modified residue

312

Phosphoserine Ref.14

Sequences

Sequence

Length

Mass (Da)

Tools

P40038 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: BD8A0C7A501C94E5
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FASTA

420

47,007

        10         20         30         40         50         60 
MSIKGDSPSS TNASSSPKST YSIQSDDKAN LGSGNVDIRT DNSQQDSNNR RDIVVVTRVA 

        70         80         90        100        110        120 
SEETLESQSS TSSMGIRPES SFNYEDASNQ ARVEMNNRVH GSNMNTINKY YPVRFPKNNE 

       130        140        150        160        170        180 
RQLSDTNNLN EKVQGTHTVQ SSTQEDKILD GDTSNSQVTP SLNIAEFPTD KLLKMLTALL 

       190        200        210        220        230        240 
TKIIKSNDRT AATNPSLTQE IENGRCLALS DNEKKYLSPV LGFRGKHVPQ IGLDQYFQRI 

       250        260        270        280        290        300 
QKYCPTTNDV FLSLLVYFDR ISKRCNSVTT TPKTNTAKHE SPSNESSLDK ANRGADKMSA 

       310        320        330        340        350        360 
CNSNENNEND DSDDENTGVQ RDSRAHPQMF VMDSHNIHRL IIAGITVSTK FLSDFFYSNS 

       370        380        390        400        410        420 
RYSRVGGISL QELNHLELQF LVLCDFELLI SVNELQRYAD LLYRFWNNAK AQSQALVTGM

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"A family of cyclin-like proteins that interact with the Pho85 cyclin-dependent kinase." Measday V., Moore L., Retnakaran R., Lee J., Donoviel M., Neiman A.M., Andrews B.J. Mol. Cell. Biol. 17:1212-1223(1997) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PHO85.
[4]	"Novel roles for elongin C in yeast." Jackson T., Kwon E., Chachulska A.M., Hyman L.E. Biochim. Biophys. Acta 1491:161-176(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ELC1.
[5]	"Regulation of the Pcl7-Pho85 cyclin-cdk complex by Pho81." Lee M., O'Regan S., Moreau J.-L., Johnson A.L., Johnston L.H., Goding C.R. Mol. Microbiol. 38:411-422(2000) [PubMed] [Europe PMC] [Abstract] Cited for: ENZYME REGULATION, INTERACTION WITH PHO81 AND PHO85.
[6]	"The yeast cyclins Pcl6p and Pcl7p are involved in the control of glycogen storage by the cyclin-dependent protein kinase Pho85p." Wang Z., Wilson W.A., Fujino M.A., Roach P.J. FEBS Lett. 506:277-280(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Analysis of phosphorylation of YJL084c, a yeast protein." Shi X.Z., Ao S.Z. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 34:433-438(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH YJL084C, PHOSPHORYLATION OF YJL084C.
[8]	"Binding to Elongin C inhibits degradation of interacting proteins in yeast." Hyman L.E., Kwon E., Ghosh S., McGee J., Chachulska A.M., Jackson T., Baricos W.H. J. Biol. Chem. 277:15586-15591(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"Pho85 phosphorylates the Glc7 protein phosphatase regulator Glc8 in vivo." Tan Y.S.H., Morcos P.A., Cannon J.F. J. Biol. Chem. 278:147-153(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION OF GLC8.
[10]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[11]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[12]	"Regulation of yeast glycogen phosphorylase by the cyclin-dependent protein kinase Pho85p." Wilson W.A., Wang Z., Roach P.J. Biochem. Biophys. Res. Commun. 329:161-167(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[13]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-25 AND SER-281, MASS SPECTROMETRY.
[14]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-21; SER-22; SER-25; SER-61; SER-281 AND SER-312, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18813 Genomic DNA. Translation: AAB64595.1. BK006939 Genomic DNA. Translation: DAA07717.1.
PIR	S50562.
RefSeq	NP_010980.1. NM_001178950.1.
3D structure databases
ProteinModelPortal	P40038.
SMR	P40038. Positions 330-389.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1496N.
IntAct	P40038. 18 interactions.
MINT	MINT-394507.
STRING	4932.YER059W.
Proteomic databases
PaxDb	P40038.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER059W; YER059W; YER059W.
GeneID	856787.
KEGG	sce:YER059W.
Organism-specific databases
CYGD	YER059w.
SGD	S000000861. PCL6.
Phylogenomic databases
eggNOG	NOG256941.
GeneTree	ENSGT00530000066532.
HOGENOM	HOG000074708.
OrthoDB	EOG451J0Z.
Enzyme and pathway databases
BioCyc	YEAST:G3O-30236-MONOMER.
Gene expression databases
Genevestigator	P40038.
GermOnline	YER059W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013922. Cyclin_PHO80-like. [Graphical view]
Pfam	PF08613. Cyclin. 2 hits. [Graphical view]
PROSITE	PS00292. CYCLINS. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	983009.

Entry information

Entry name

PCL6_YEAST

Accession

Primary (citable) accession number: P40038
Secondary accession number(s): D3DLW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 29, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents