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Protein

PHO85 cyclin-6

Gene

PCL6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cyclin partner of the cyclin-dependent kinase (CDK) PHO85. Together with cyclin PCL7, controls glycogen phosphorylase and glycogen synthase activities in response to nutrient availablility. The PCL6-PHO85 cyclin-CDK holoenzyme has GLC8 kinase activity and phosphorylates and inactivates the phosphatase PP1-2 inhibitor GLC8, causing activation of PP1-2, which then dephosphorylates and activates glycogen phosphorylase. PCL6-PHO85 also phosphorylates YJL084C.5 Publications

GO - Molecular functioni

  1. cyclin-dependent protein serine/threonine kinase regulator activity Source: SGD

GO - Biological processi

  1. glycogen metabolic process Source: UniProtKB-KW
  2. regulation of cyclin-dependent protein serine/threonine kinase activity Source: InterPro
  3. regulation of glycogen biosynthetic process Source: SGD
  4. regulation of glycogen catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Cyclin

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Enzyme and pathway databases

BioCyciYEAST:G3O-30236-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
PHO85 cyclin-6
Gene namesi
Name:PCL6
Ordered Locus Names:YER059W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER059w.
SGDiS000000861. PCL6.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. cyclin-dependent protein kinase holoenzyme complex Source: SGD
  2. cytoplasm Source: UniProtKB-SubCell
  3. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 420420PHO85 cyclin-6PRO_0000202631Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei61 – 611Phosphoserine1 Publication
Modified residuei281 – 2811Phosphoserine1 Publication
Modified residuei312 – 3121Phosphoserine2 Publications
Modified residuei317 – 3171Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40038.
PaxDbiP40038.

Expressioni

Gene expression databases

GenevestigatoriP40038.

Interactioni

Subunit structurei

Forms a cyclin-CDK complex with PHO85. Interacts with the substrate protein YJL084C. Interacts with elongin-C, which stabilizes PCL6. Interacts with the CDK inhibitor (CKI) PHO81.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PHO85P171577EBI-22555,EBI-13327

Protein-protein interaction databases

BioGridi36800. 36 interactions.
DIPiDIP-1496N.
IntActiP40038. 20 interactions.
MINTiMINT-394507.
STRINGi4932.YER059W.

Structurei

3D structure databases

ProteinModelPortaliP40038.
SMRiP40038. Positions 332-395.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cyclin family. PHO80 subfamily.Curated

Phylogenomic databases

eggNOGiNOG256941.
GeneTreeiENSGT00530000066532.
HOGENOMiHOG000074708.
InParanoidiP40038.
OrthoDBiEOG7PP5HV.

Family and domain databases

InterProiIPR013922. Cyclin_PHO80-like.
[Graphical view]
PfamiPF08613. Cyclin. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40038-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIKGDSPSS TNASSSPKST YSIQSDDKAN LGSGNVDIRT DNSQQDSNNR
60 70 80 90 100
RDIVVVTRVA SEETLESQSS TSSMGIRPES SFNYEDASNQ ARVEMNNRVH
110 120 130 140 150
GSNMNTINKY YPVRFPKNNE RQLSDTNNLN EKVQGTHTVQ SSTQEDKILD
160 170 180 190 200
GDTSNSQVTP SLNIAEFPTD KLLKMLTALL TKIIKSNDRT AATNPSLTQE
210 220 230 240 250
IENGRCLALS DNEKKYLSPV LGFRGKHVPQ IGLDQYFQRI QKYCPTTNDV
260 270 280 290 300
FLSLLVYFDR ISKRCNSVTT TPKTNTAKHE SPSNESSLDK ANRGADKMSA
310 320 330 340 350
CNSNENNEND DSDDENTGVQ RDSRAHPQMF VMDSHNIHRL IIAGITVSTK
360 370 380 390 400
FLSDFFYSNS RYSRVGGISL QELNHLELQF LVLCDFELLI SVNELQRYAD
410 420
LLYRFWNNAK AQSQALVTGM
Length:420
Mass (Da):47,007
Last modified:February 1, 1995 - v1
Checksum:iBD8A0C7A501C94E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18813 Genomic DNA. Translation: AAB64595.1.
BK006939 Genomic DNA. Translation: DAA07717.1.
PIRiS50562.
RefSeqiNP_010980.1. NM_001178950.1.

Genome annotation databases

EnsemblFungiiYER059W; YER059W; YER059W.
GeneIDi856787.
KEGGisce:YER059W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18813 Genomic DNA. Translation: AAB64595.1.
BK006939 Genomic DNA. Translation: DAA07717.1.
PIRiS50562.
RefSeqiNP_010980.1. NM_001178950.1.

3D structure databases

ProteinModelPortaliP40038.
SMRiP40038. Positions 332-395.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36800. 36 interactions.
DIPiDIP-1496N.
IntActiP40038. 20 interactions.
MINTiMINT-394507.
STRINGi4932.YER059W.

Proteomic databases

MaxQBiP40038.
PaxDbiP40038.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER059W; YER059W; YER059W.
GeneIDi856787.
KEGGisce:YER059W.

Organism-specific databases

CYGDiYER059w.
SGDiS000000861. PCL6.

Phylogenomic databases

eggNOGiNOG256941.
GeneTreeiENSGT00530000066532.
HOGENOMiHOG000074708.
InParanoidiP40038.
OrthoDBiEOG7PP5HV.

Enzyme and pathway databases

BioCyciYEAST:G3O-30236-MONOMER.

Miscellaneous databases

NextBioi983009.

Gene expression databases

GenevestigatoriP40038.

Family and domain databases

InterProiIPR013922. Cyclin_PHO80-like.
[Graphical view]
PfamiPF08613. Cyclin. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "A family of cyclin-like proteins that interact with the Pho85 cyclin-dependent kinase."
    Measday V., Moore L., Retnakaran R., Lee J., Donoviel M., Neiman A.M., Andrews B.J.
    Mol. Cell. Biol. 17:1212-1223(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PHO85.
  4. Cited for: INTERACTION WITH ELC1.
  5. Cited for: ENZYME REGULATION, INTERACTION WITH PHO81 AND PHO85.
  6. "The yeast cyclins Pcl6p and Pcl7p are involved in the control of glycogen storage by the cyclin-dependent protein kinase Pho85p."
    Wang Z., Wilson W.A., Fujino M.A., Roach P.J.
    FEBS Lett. 506:277-280(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Analysis of phosphorylation of YJL084c, a yeast protein."
    Shi X.Z., Ao S.Z.
    Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 34:433-438(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH YJL084C, PHOSPHORYLATION OF YJL084C.
  8. "Binding to Elongin C inhibits degradation of interacting proteins in yeast."
    Hyman L.E., Kwon E., Ghosh S., McGee J., Chachulska A.M., Jackson T., Baricos W.H.
    J. Biol. Chem. 277:15586-15591(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Pho85 phosphorylates the Glc7 protein phosphatase regulator Glc8 in vivo."
    Tan Y.S.H., Morcos P.A., Cannon J.F.
    J. Biol. Chem. 278:147-153(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION OF GLC8.
  10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  12. "Regulation of yeast glycogen phosphorylase by the cyclin-dependent protein kinase Pho85p."
    Wilson W.A., Wang Z., Roach P.J.
    Biochem. Biophys. Res. Commun. 329:161-167(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-61; SER-281 AND SER-312, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-312 AND THR-317, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPCL6_YEAST
AccessioniPrimary (citable) accession number: P40038
Secondary accession number(s): D3DLW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: January 7, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1240 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.