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P40038

- PCL6_YEAST

UniProt

P40038 - PCL6_YEAST

Protein

PHO85 cyclin-6

Gene

PCL6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Cyclin partner of the cyclin-dependent kinase (CDK) PHO85. Together with cyclin PCL7, controls glycogen phosphorylase and glycogen synthase activities in response to nutrient availablility. The PCL6-PHO85 cyclin-CDK holoenzyme has GLC8 kinase activity and phosphorylates and inactivates the phosphatase PP1-2 inhibitor GLC8, causing activation of PP1-2, which then dephosphorylates and activates glycogen phosphorylase. PCL6-PHO85 also phosphorylates YJL084C.5 Publications

    GO - Molecular functioni

    1. cyclin-dependent protein serine/threonine kinase regulator activity Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. glycogen metabolic process Source: UniProtKB-KW
    2. regulation of cyclin-dependent protein serine/threonine kinase activity Source: InterPro
    3. regulation of glycogen biosynthetic process Source: SGD
    4. regulation of glycogen catabolic process Source: SGD

    Keywords - Molecular functioni

    Cyclin

    Keywords - Biological processi

    Carbohydrate metabolism, Glycogen metabolism

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30236-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    PHO85 cyclin-6
    Gene namesi
    Name:PCL6
    Ordered Locus Names:YER059W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYER059w.
    SGDiS000000861. PCL6.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cyclin-dependent protein kinase holoenzyme complex Source: SGD
    2. cytoplasm Source: UniProtKB-SubCell
    3. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 420420PHO85 cyclin-6PRO_0000202631Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei61 – 611Phosphoserine1 Publication
    Modified residuei281 – 2811Phosphoserine1 Publication
    Modified residuei312 – 3121Phosphoserine2 Publications
    Modified residuei317 – 3171Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP40038.
    PaxDbiP40038.

    Expressioni

    Gene expression databases

    GenevestigatoriP40038.

    Interactioni

    Subunit structurei

    Forms a cyclin-CDK complex with PHO85. Interacts with the substrate protein YJL084C. Interacts with elongin-C, which stabilizes PCL6. Interacts with the CDK inhibitor (CKI) PHO81.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PHO85P171577EBI-22555,EBI-13327

    Protein-protein interaction databases

    BioGridi36800. 36 interactions.
    DIPiDIP-1496N.
    IntActiP40038. 20 interactions.
    MINTiMINT-394507.
    STRINGi4932.YER059W.

    Structurei

    3D structure databases

    ProteinModelPortaliP40038.
    SMRiP40038. Positions 332-395.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cyclin family. PHO80 subfamily.Curated

    Phylogenomic databases

    eggNOGiNOG256941.
    GeneTreeiENSGT00530000066532.
    HOGENOMiHOG000074708.
    OrthoDBiEOG7PP5HV.

    Family and domain databases

    InterProiIPR013922. Cyclin_PHO80-like.
    [Graphical view]
    PfamiPF08613. Cyclin. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40038-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSIKGDSPSS TNASSSPKST YSIQSDDKAN LGSGNVDIRT DNSQQDSNNR    50
    RDIVVVTRVA SEETLESQSS TSSMGIRPES SFNYEDASNQ ARVEMNNRVH 100
    GSNMNTINKY YPVRFPKNNE RQLSDTNNLN EKVQGTHTVQ SSTQEDKILD 150
    GDTSNSQVTP SLNIAEFPTD KLLKMLTALL TKIIKSNDRT AATNPSLTQE 200
    IENGRCLALS DNEKKYLSPV LGFRGKHVPQ IGLDQYFQRI QKYCPTTNDV 250
    FLSLLVYFDR ISKRCNSVTT TPKTNTAKHE SPSNESSLDK ANRGADKMSA 300
    CNSNENNEND DSDDENTGVQ RDSRAHPQMF VMDSHNIHRL IIAGITVSTK 350
    FLSDFFYSNS RYSRVGGISL QELNHLELQF LVLCDFELLI SVNELQRYAD 400
    LLYRFWNNAK AQSQALVTGM 420
    Length:420
    Mass (Da):47,007
    Last modified:February 1, 1995 - v1
    Checksum:iBD8A0C7A501C94E5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18813 Genomic DNA. Translation: AAB64595.1.
    BK006939 Genomic DNA. Translation: DAA07717.1.
    PIRiS50562.
    RefSeqiNP_010980.1. NM_001178950.1.

    Genome annotation databases

    EnsemblFungiiYER059W; YER059W; YER059W.
    GeneIDi856787.
    KEGGisce:YER059W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18813 Genomic DNA. Translation: AAB64595.1 .
    BK006939 Genomic DNA. Translation: DAA07717.1 .
    PIRi S50562.
    RefSeqi NP_010980.1. NM_001178950.1.

    3D structure databases

    ProteinModelPortali P40038.
    SMRi P40038. Positions 332-395.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36800. 36 interactions.
    DIPi DIP-1496N.
    IntActi P40038. 20 interactions.
    MINTi MINT-394507.
    STRINGi 4932.YER059W.

    Proteomic databases

    MaxQBi P40038.
    PaxDbi P40038.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER059W ; YER059W ; YER059W .
    GeneIDi 856787.
    KEGGi sce:YER059W.

    Organism-specific databases

    CYGDi YER059w.
    SGDi S000000861. PCL6.

    Phylogenomic databases

    eggNOGi NOG256941.
    GeneTreei ENSGT00530000066532.
    HOGENOMi HOG000074708.
    OrthoDBi EOG7PP5HV.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30236-MONOMER.

    Miscellaneous databases

    NextBioi 983009.

    Gene expression databases

    Genevestigatori P40038.

    Family and domain databases

    InterProi IPR013922. Cyclin_PHO80-like.
    [Graphical view ]
    Pfami PF08613. Cyclin. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "A family of cyclin-like proteins that interact with the Pho85 cyclin-dependent kinase."
      Measday V., Moore L., Retnakaran R., Lee J., Donoviel M., Neiman A.M., Andrews B.J.
      Mol. Cell. Biol. 17:1212-1223(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PHO85.
    4. Cited for: INTERACTION WITH ELC1.
    5. Cited for: ENZYME REGULATION, INTERACTION WITH PHO81 AND PHO85.
    6. "The yeast cyclins Pcl6p and Pcl7p are involved in the control of glycogen storage by the cyclin-dependent protein kinase Pho85p."
      Wang Z., Wilson W.A., Fujino M.A., Roach P.J.
      FEBS Lett. 506:277-280(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Analysis of phosphorylation of YJL084c, a yeast protein."
      Shi X.Z., Ao S.Z.
      Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 34:433-438(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH YJL084C, PHOSPHORYLATION OF YJL084C.
    8. "Binding to Elongin C inhibits degradation of interacting proteins in yeast."
      Hyman L.E., Kwon E., Ghosh S., McGee J., Chachulska A.M., Jackson T., Baricos W.H.
      J. Biol. Chem. 277:15586-15591(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Pho85 phosphorylates the Glc7 protein phosphatase regulator Glc8 in vivo."
      Tan Y.S.H., Morcos P.A., Cannon J.F.
      J. Biol. Chem. 278:147-153(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION OF GLC8.
    10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    12. "Regulation of yeast glycogen phosphorylase by the cyclin-dependent protein kinase Pho85p."
      Wilson W.A., Wang Z., Roach P.J.
      Biochem. Biophys. Res. Commun. 329:161-167(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    13. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-61; SER-281 AND SER-312, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-312 AND THR-317, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPCL6_YEAST
    AccessioniPrimary (citable) accession number: P40038
    Secondary accession number(s): D3DLW3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1240 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3