P40036 (GIP2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GLC7-interacting protein 2

Gene names

Name:	GIP2
Ordered Locus Names:	YER054C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with phosphatase 1 (GLC7). Ref.3
Miscellaneous	Present with 125 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Contains 1 CBM21 (carbohydrate binding type-21) domain.

Ontologies

Keywords
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycogen metabolic process Inferred from mutant phenotype. Source: SGD protein dephosphorylation Inferred from physical interaction. Source: SGD
Cellular component	protein phosphatase type 1 complex Inferred from physical interaction. Source: SGD
Molecular function	protein phosphatase regulator activity Inferred from physical interaction. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 548	548	GLC7-interacting protein 2		PRO_0000071521
Regions
	Domain	419 – 534	116	CBM21
Amino acid modifications
	Modified residue	51	1	Phosphoserine Ref.7
	Modified residue	137	1	Phosphoserine Ref.7
	Modified residue	194	1	Phosphoserine Ref.6 Ref.7
	Modified residue	219	1	Phosphoserine Ref.7
	Modified residue	221	1	Phosphoserine Ref.5 Ref.7
	Modified residue	238	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P40036 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: B28EE5241989A9BF	FASTA	548	62,319
	10 20 30 40 50 60 MYIKAEQKPQ QFERKNEKLD RNKNQQLPDL ETDFKGYRVN SDLYNKERDG STEETLNSLK 70 80 90 100 110 120 FLHKPQRVTQ MRANRFPEEE VQRNTDLNKR IFSAGNDENV DNESGWSKIA AAKNHTSVES 130 140 150 160 170 180 LNGSTRPPFK IELPPLSPKS TVPKSFQAEY PEAKSPGNDM NFEYDEEILI PFAPPVYKKS 190 200 210 220 230 240 GELLKSSLKR RSKSLPTTPG IRSGNGVQAR DGSPMLIRSK SVHFDQAAPV KYFAEDESPI 250 260 270 280 290 300 NVNKTEQHDN CLSFKHKPVN LMVDPEEETK MLSSGLETTS IDDDLTTVAP KGFAHPAKIS 310 320 330 340 350 360 NPNNGKGTNN TKLRKSKRFQ NLLKNRTDMP PSKSNKKFVN GGGAHEISDR NSKNYHVVGL 370 380 390 400 410 420 YSKNFPILSN KNPKSLKLNI FINLSQNKKV FLQELSLYIH RDNNYFSNSS SFYNIPNSHN 430 440 450 460 470 480 GNDCNGVAKG YNAGCTRLIA GRILVKNIFY DKRVVVRYTW DSWRTTHEVE CVYISDGDGI 490 500 510 520 530 540 LPGTNMDIFH FIIDDVSKVD PRGKLEFCIH YSTRNDYERE EYWDNNNGNN YKVDVVMDGF NDPFAAAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	Tu J., Song W., Carslon R. Unpublished observations (MAR-1996) Cited for: INTERACTION WITH GLC7.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-221, MASS SPECTROMETRY.
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-194, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-51; SER-137; SER-194; SER-219; SER-221 AND SER-238, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18813 Genomic DNA. Translation: AAB64590.1. BK006939 Genomic DNA. Translation: DAA07711.1.
PIR	S50557.
RefSeq	NP_010974.1. NM_001178945.1.
3D structure databases
ProteinModelPortal	P40036.
SMR	P40036. Positions 381-545.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2376N.
IntAct	P40036. 13 interactions.
MINT	MINT-600077.
STRING	P40036.
Protein family/group databases
CAZy	CBM21. Carbohydrate-Binding Module Family 21.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER054C; YER054C; YER054C.
GeneID	856781.
KEGG	sce:YER054C.
NMPDR	fig|4932.3.peg.2038.
Organism-specific databases
CYGD	YER054c.
SGD	S000000856. GIP2.
Phylogenomic databases
eggNOG	fuNOG10916.
GeneTree	EFGT00050000003028.
HOGENOM	HBG203665.
OMA	LSNKNPK.
OrthoDB	EOG45TGXJ.
Gene expression databases
ArrayExpress	P40036.
Genevestigator	P40036.
GermOnline	YER054C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005036. CBM_21. IPR016717. Pase-1_int. [Graphical view]
Pfam	PF03370. CBM_21. 1 hit. [Graphical view]
PIRSF	PIRSF018234. PPase_interacting. 1 hit.
PROSITE	PS51159. CBM21. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982991.

Entry information

Entry name

GIP2_YEAST

Accession

Primary (citable) accession number: P40036
Secondary accession number(s): D3DLV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program