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Reviewed, UniProtKB/Swiss-Prot P40030 (ERG28_YEAST)

Last modified June 16, 2009. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Ergosterol biosynthetic protein 28
Gene names
Name: ERG28
Synonyms: BUD18
Ordered Locus Names: YER044C
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length148 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Has a role as a scaffold to help anchor ERG25, ERG26 and ERG27 to the endoplasmic reticulum. May also be responsible for facilitating their interaction. Ref.3

Subunit structure

Heterotetramer of ERG25, ERG26, ERG27 and ERG28. ERG28 acts as a scaffold to tether ERG27 and other 4,4-demethylation-related enzymes, forming a demethylation enzyme complex, in the endoplasmic reticulum. Interacts with ERG25, ERG26 and ERG27. Also interacts with ERG1, ERG3, ERG5, ERG6 and ERG11. Ref.4 Ref.6 Ref.7

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Probable.

Miscellaneous

Present with 377 molecules/cell in log phase SD medium. Ref.5

Sequence similarities

Belongs to the ERG28 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 148148Ergosterol biosynthetic protein 28
PRO_0000193908

Regions

Topological domain1 – 2525Cytoplasmic Potential
Transmembrane26 – 4621 Potential
Topological domain47 – 9246Lumenal Potential
Transmembrane93 – 11321 Potential
Topological domain114 – 1207Cytoplasmic Potential
Transmembrane121 – 13616 Potential
Topological domain137 – 14812Lumenal Potential

Sequences

Sequence LengthMass (Da)Tools
P40030-1 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: F1BD39AFA180E367

FASTA14817,135
        10         20         30         40         50         60 
MFSLQDVITT TKTTLAAMPK GYLPKWLLFI SIVSVFNSIQ TYVSGLELTR KVYERKPTET 

        70         80         90        100        110        120 
THLSARTFGT WTFISCVIRF YGAMYLNEPH IFELVFMSYM VALFHFGSEL LIFRTCKLGK 

       130        140 
GFMGPLVVST TSLVWMYKQR EYYTGVAW 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed: 9169868] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"A novel gene conserved from yeast to humans is involved in sterol biosynthesis."
Gachotte D., Eckstein J., Barbuch R., Hughes T., Roberts C., Bard M.
J. Lipid Res. 42:150-154(2001) [PubMed: 11160377] [Abstract]
Cited for: FUNCTION.
[4]"Protein-protein interactions among C-4 demethylation enzymes involved in yeast sterol biosynthesis."
Mo C., Valachovic M., Randall S.K., Nickels J.T., Bard M.
Proc. Natl. Acad. Sci. U.S.A. 99:9739-9744(2002) [PubMed: 12119386] [Abstract]
Cited for: SUBUNIT, INTERACTION WITH ERG25 AND ERG27, SUBCELLULAR LOCATION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"The ERG28-encoded protein, Erg28p, interacts with both the sterol C-4 demethylation enzyme complex as well as the late biosynthetic protein, the C-24 sterol methyltransferase (Erg6p)."
Mo C., Valachovic M., Bard M.
Biochim. Biophys. Acta 1686:30-36(2004) [PubMed: 15522820] [Abstract]
Cited for: INTERACTION WITH ERG6.
[7]"Erg28p is a key protein in the yeast sterol biosynthetic enzyme complex."
Mo C., Bard M.
J. Lipid Res. 46:1991-1998(2005) [PubMed: 15995173] [Abstract]
Cited for: INTERACTION WITH ERG1; ERG3; ERG5; ERG6; ERG11; ERG25; ERG26 AND ERG27.
[8]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

U18796 Genomic DNA. Translation: AAB64579.1.
AY558453 Genomic DNA. Translation: AAS56779.1.
PIRS50547.
RefSeqNP_010962.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:2672N.
IntActP40030. 19 interactions.

Proteomic databases

PeptideAtlasP40030.

Genome annotation databases

EnsemblYER044C. Saccharomyces cerevisiae. [Contig view]
GeneID856767.
GenomeReviewsGene locus YER044C in contig U00092_GR.
KEGGsce:YER044C.
NMPDRfig|4932.3.peg.2024.

Organism-specific databases

CYGDYER044c.
SGDS000000846. ERG28.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMP40030.
OMAP40030. RTLYHIT.

Gene expression databases

GermOnlineYER044C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR005352. Erg28.
[Graphical view]
PfamPF03694. Erg28. 1 hit.
[Graphical view]
ProDomPD089646. UPF0143. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Other Resources

DrugBankDB00681. Amphotericin B.
DB04794. Bifonazole.
DB01152. Candicidin.
DB00826. Natamycin.
DB00239. Oxiconazole.
NextBio982950.

Entry information

Entry nameERG28_YEAST
AccessionPrimary (citable) accession number: P40030
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 16, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Uncharacterized protein families (UPF)

List of uncharacterized protein family (UPF) entries

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents