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Protein

Ergosterol biosynthetic protein 28

Gene

ERG28

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has a role as a scaffold to help anchor ERG25, ERG26 and ERG27 to the endoplasmic reticulum. May also be responsible for facilitating their interaction.1 Publication

GO - Molecular functioni

  • protein binding, bridging Source: SGD

GO - Biological processi

  • ergosterol biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14347.
YEAST:G3O-30223-MONOMER.
YEAST:MONOMER3O-271.

Names & Taxonomyi

Protein namesi
Recommended name:
Ergosterol biosynthetic protein 28
Gene namesi
Name:ERG28
Synonyms:BUD18
Ordered Locus Names:YER044C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER044C.
SGDiS000000846. ERG28.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2525CytoplasmicSequence analysisAdd
BLAST
Transmembranei26 – 4621HelicalSequence analysisAdd
BLAST
Topological domaini47 – 9246LumenalSequence analysisAdd
BLAST
Transmembranei93 – 11321HelicalSequence analysisAdd
BLAST
Topological domaini114 – 1207CytoplasmicSequence analysis
Transmembranei121 – 13616HelicalSequence analysisAdd
BLAST
Topological domaini137 – 14812LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 148148Ergosterol biosynthetic protein 28PRO_0000193908Add
BLAST

Proteomic databases

MaxQBiP40030.

Interactioni

Subunit structurei

Heterotetramer of ERG25, ERG26, ERG27 and ERG28. ERG28 acts as a scaffold to tether ERG27 and other 4,4-demethylation-related enzymes, forming a demethylation enzyme complex, in the endoplasmic reticulum. Interacts with ERG25, ERG26 and ERG27. Also interacts with ERG1, ERG3, ERG5, ERG6 and ERG11.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ERG25P530453EBI-22518,EBI-6506
ERG26P531993EBI-22518,EBI-6514
ERG27Q124526EBI-22518,EBI-38132

GO - Molecular functioni

  • protein binding, bridging Source: SGD

Protein-protein interaction databases

BioGridi36780. 28 interactions.
DIPiDIP-2672N.
IntActiP40030. 12 interactions.
MINTiMINT-510893.

Structurei

3D structure databases

ProteinModelPortaliP40030.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ERG28 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000010925.
HOGENOMiHOG000164093.
InParanoidiP40030.
OMAiVQSFRDH.
OrthoDBiEOG7SV173.

Family and domain databases

InterProiIPR005352. Erg28.
[Graphical view]
PfamiPF03694. Erg28. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40030-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSLQDVITT TKTTLAAMPK GYLPKWLLFI SIVSVFNSIQ TYVSGLELTR
60 70 80 90 100
KVYERKPTET THLSARTFGT WTFISCVIRF YGAMYLNEPH IFELVFMSYM
110 120 130 140
VALFHFGSEL LIFRTCKLGK GFMGPLVVST TSLVWMYKQR EYYTGVAW
Length:148
Mass (Da):17,135
Last modified:February 1, 1995 - v1
Checksum:iF1BD39AFA180E367
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18796 Genomic DNA. Translation: AAB64579.1.
AY558453 Genomic DNA. Translation: AAS56779.1.
BK006939 Genomic DNA. Translation: DAA07698.1.
PIRiS50547.
RefSeqiNP_010962.1. NM_001178935.1.

Genome annotation databases

EnsemblFungiiYER044C; YER044C; YER044C.
GeneIDi856767.
KEGGisce:YER044C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18796 Genomic DNA. Translation: AAB64579.1.
AY558453 Genomic DNA. Translation: AAS56779.1.
BK006939 Genomic DNA. Translation: DAA07698.1.
PIRiS50547.
RefSeqiNP_010962.1. NM_001178935.1.

3D structure databases

ProteinModelPortaliP40030.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36780. 28 interactions.
DIPiDIP-2672N.
IntActiP40030. 12 interactions.
MINTiMINT-510893.

Proteomic databases

MaxQBiP40030.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER044C; YER044C; YER044C.
GeneIDi856767.
KEGGisce:YER044C.

Organism-specific databases

EuPathDBiFungiDB:YER044C.
SGDiS000000846. ERG28.

Phylogenomic databases

GeneTreeiENSGT00390000010925.
HOGENOMiHOG000164093.
InParanoidiP40030.
OMAiVQSFRDH.
OrthoDBiEOG7SV173.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14347.
YEAST:G3O-30223-MONOMER.
YEAST:MONOMER3O-271.

Miscellaneous databases

PROiP40030.

Family and domain databases

InterProiIPR005352. Erg28.
[Graphical view]
PfamiPF03694. Erg28. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "A novel gene conserved from yeast to humans is involved in sterol biosynthesis."
    Gachotte D., Eckstein J., Barbuch R., Hughes T., Roberts C., Bard M.
    J. Lipid Res. 42:150-154(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Protein-protein interactions among C-4 demethylation enzymes involved in yeast sterol biosynthesis."
    Mo C., Valachovic M., Randall S.K., Nickels J.T., Bard M.
    Proc. Natl. Acad. Sci. U.S.A. 99:9739-9744(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, INTERACTION WITH ERG25 AND ERG27, SUBCELLULAR LOCATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "The ERG28-encoded protein, Erg28p, interacts with both the sterol C-4 demethylation enzyme complex as well as the late biosynthetic protein, the C-24 sterol methyltransferase (Erg6p)."
    Mo C., Valachovic M., Bard M.
    Biochim. Biophys. Acta 1686:30-36(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ERG6.
  8. "Erg28p is a key protein in the yeast sterol biosynthetic enzyme complex."
    Mo C., Bard M.
    J. Lipid Res. 46:1991-1998(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ERG1; ERG3; ERG5; ERG6; ERG11; ERG25; ERG26 AND ERG27.
  9. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiERG28_YEAST
AccessioniPrimary (citable) accession number: P40030
Secondary accession number(s): D3DLU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 6, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 377 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.