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Protein

Holliday junction resolvase YEN1

Gene

YEN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Endonuclease which resolves Holliday junctions by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation. Involved in DNA-damage repair in vegetative cells.3 Publications

GO - Molecular functioni

  • crossover junction endodeoxyribonuclease activity Source: SGD
  • DNA binding Source: InterPro
  • single-stranded DNA endodeoxyribonuclease activity Source: SGD

GO - Biological processi

  • cellular response to DNA damage stimulus Source: SGD
  • DNA repair Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Enzyme and pathway databases

BioCyciYEAST:G3O-30222-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Holliday junction resolvase YEN1 (EC:3.1.-.-)
Gene namesi
Name:YEN1
Ordered Locus Names:YER041W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER041W.
SGDiS000000843. YEN1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002026291 – 759Holliday junction resolvase YEN1Add BLAST759

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei730PhosphoserineCombined sources1
Modified residuei731PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40028.
PRIDEiP40028.

PTM databases

iPTMnetiP40028.

Interactioni

Protein-protein interaction databases

BioGridi36777. 23 interactors.
DIPiDIP-6450N.
IntActiP40028. 1 interactor.
MINTiMINT-680602.

Structurei

3D structure databases

ProteinModelPortaliP40028.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000142381.
InParanoidiP40028.
KOiK15339.
OMAiWLFECGF.
OrthoDBiEOG092C0W33.

Family and domain databases

Gene3Di3.40.50.1010. 1 hit.
InterProiIPR020045. 5-3_exonuclease_C.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR006084. XPG/Rad2.
IPR006085. XPG_DNA_repair_N.
[Graphical view]
PANTHERiPTHR11081. PTHR11081. 4 hits.
PfamiPF00867. XPG_I. 1 hit.
[Graphical view]
PRINTSiPR00853. XPGRADSUPER.
SMARTiSM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 2 hits.
SSF88723. SSF88723. 2 hits.

Sequencei

Sequence statusi: Complete.

P40028-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVSQIWEFL KPYLQDSRIP LRKFVIDFNK SQKRAPRIAI DAYGWLFECG
60 70 80 90 100
FIQNIDISAR SRSRSRSPTR SPRDSDIDSS QEYYGSRSYT TTGKAVINFI
110 120 130 140 150
SRLKELLSLN VEFLLVFDGV MKPSFKRKFN HEQNATTCDD EKEYYSSWEQ
160 170 180 190 200
HVKNHEVYGN CKGLLAPSDP EFISLVRKLL DLMNISYVIA CGEGEAQCVW
210 220 230 240 250
LQVSGAVDFI LSNDSDTLVF GGEKILKNYS KFYDDFGPSS ITSHSPSRHH
260 270 280 290 300
DSKESFVTVI DLPKINKVAG KKFDRLSLLF FSVLLGADYN RGVKGLGKNK
310 320 330 340 350
SLQLAQCEDP NFSMEFYDIF KDFNLEDLTS ESLRKSRYRL FQKRLYLYCK
360 370 380 390 400
DHSVELFGRN YPVLLNQGSF EGWPSTVAIM HYFHPIVQPY FDEEVLSDKY
410 420 430 440 450
INMAGNGHYR NLNFNELKYF LQSLNLPQIS SFDKWFHDSM HEMFLLREFL
460 470 480 490 500
SIDESDNIGK GNMRITEEKI MNIDGGKFQI PCFKIRYTTF LPNIPISSQS
510 520 530 540 550
PLKRSNSPSR SKSPTRRQMD IMEHPNSLWL PKYLIPQSHP LVIQYYETQQ
560 570 580 590 600
LIQKEKEKKG KKSNKSRLPQ KNNLDEFLRK HTSPIKSIGK VGESRKEILE
610 620 630 640 650
PVRKRLFVDT DEDTSLEEIP APTRLTTVDE HSDNDDDSLI FVDEITNSQS
660 670 680 690 700
VLDSSPGKRI RDLTQDEQVD VWKDVIEISP IKKSRTTNAE KNPPESGLKS
710 720 730 740 750
RSSITINARL QGTKMLPPNL TAPRLEREHS SVLDQLVTDA QDTVDRFVAC

DSDSSSTIE
Length:759
Mass (Da):87,364
Last modified:July 27, 2011 - v2
Checksum:iCE1305A9F78CA300
GO

Sequence cautioni

The sequence AAA60376 differs from that shown. Reason: Frameshift at positions 63, 673 and 759.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti59A → P in AAB64576 (PubMed:9169868).Curated1
Sequence conflicti164 – 165LL → FV in AAA60376 (Ref. 1) Curated2
Sequence conflicti346Y → C in AAA60376 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13615 Genomic DNA. Translation: AAA60376.1. Frameshift.
U18796 Genomic DNA. Translation: AAB64576.1.
BK006939 Genomic DNA. Translation: DAA07695.2.
PIRiS50544.
RefSeqiNP_010959.2. NM_001178932.2.

Genome annotation databases

EnsemblFungiiYER041W; YER041W; YER041W.
GeneIDi856764.
KEGGisce:YER041W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13615 Genomic DNA. Translation: AAA60376.1. Frameshift.
U18796 Genomic DNA. Translation: AAB64576.1.
BK006939 Genomic DNA. Translation: DAA07695.2.
PIRiS50544.
RefSeqiNP_010959.2. NM_001178932.2.

3D structure databases

ProteinModelPortaliP40028.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36777. 23 interactors.
DIPiDIP-6450N.
IntActiP40028. 1 interactor.
MINTiMINT-680602.

PTM databases

iPTMnetiP40028.

Proteomic databases

MaxQBiP40028.
PRIDEiP40028.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER041W; YER041W; YER041W.
GeneIDi856764.
KEGGisce:YER041W.

Organism-specific databases

EuPathDBiFungiDB:YER041W.
SGDiS000000843. YEN1.

Phylogenomic databases

HOGENOMiHOG000142381.
InParanoidiP40028.
KOiK15339.
OMAiWLFECGF.
OrthoDBiEOG092C0W33.

Enzyme and pathway databases

BioCyciYEAST:G3O-30222-MONOMER.

Miscellaneous databases

PROiP40028.

Family and domain databases

Gene3Di3.40.50.1010. 1 hit.
InterProiIPR020045. 5-3_exonuclease_C.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR006084. XPG/Rad2.
IPR006085. XPG_DNA_repair_N.
[Graphical view]
PANTHERiPTHR11081. PTHR11081. 4 hits.
PfamiPF00867. XPG_I. 1 hit.
[Graphical view]
PRINTSiPR00853. XPGRADSUPER.
SMARTiSM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 2 hits.
SSF88723. SSF88723. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiYEN1_YEAST
AccessioniPrimary (citable) accession number: P40028
Secondary accession number(s): D3DLU1, Q02463
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 131 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.