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Protein

Holliday junction resolvase YEN1

Gene

YEN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endonuclease which resolves Holliday junctions by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation. Involved in DNA-damage repair in vegetative cells.3 Publications

Miscellaneous

Present with 131 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • crossover junction endodeoxyribonuclease activity Source: SGD
  • single-stranded DNA endodeoxyribonuclease activity Source: SGD

GO - Biological processi

  • cellular response to DNA damage stimulus Source: SGD
  • DNA repair Source: SGD

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair

Enzyme and pathway databases

BioCyciYEAST:G3O-30222-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Holliday junction resolvase YEN1 (EC:3.1.-.-)
Gene namesi
Name:YEN1
Ordered Locus Names:YER041W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER041W
SGDiS000000843 YEN1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002026291 – 759Holliday junction resolvase YEN1Add BLAST759

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei730PhosphoserineCombined sources1
Modified residuei731PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40028
PaxDbiP40028
PRIDEiP40028

PTM databases

iPTMnetiP40028

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
RPN1P387642EBI-22514,EBI-15913

Protein-protein interaction databases

BioGridi36777, 45 interactors
DIPiDIP-6450N
IntActiP40028, 16 interactors
MINTiP40028
STRINGi4932.YER041W

Structurei

3D structure databases

ProteinModelPortaliP40028
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000142381
InParanoidiP40028
KOiK15339
OMAiWLFECGF
OrthoDBiEOG092C0W33

Family and domain databases

CDDicd09906 H3TH_YEN1, 1 hit
InterProiView protein in InterPro
IPR036279 5-3_exonuclease_C_sf
IPR029060 PIN-like_dom_sf
IPR006086 XPG-I_dom
IPR006084 XPG/Rad2
IPR006085 XPG_DNA_repair_N
IPR037316 Yen1_H3TH
PANTHERiPTHR11081 PTHR11081, 1 hit
PfamiView protein in Pfam
PF00867 XPG_I, 1 hit
PRINTSiPR00853 XPGRADSUPER
SMARTiView protein in SMART
SM00484 XPGI, 1 hit
SM00485 XPGN, 1 hit
SUPFAMiSSF47807 SSF47807, 2 hits
SSF88723 SSF88723, 2 hits

Sequencei

Sequence statusi: Complete.

P40028-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVSQIWEFL KPYLQDSRIP LRKFVIDFNK SQKRAPRIAI DAYGWLFECG
60 70 80 90 100
FIQNIDISAR SRSRSRSPTR SPRDSDIDSS QEYYGSRSYT TTGKAVINFI
110 120 130 140 150
SRLKELLSLN VEFLLVFDGV MKPSFKRKFN HEQNATTCDD EKEYYSSWEQ
160 170 180 190 200
HVKNHEVYGN CKGLLAPSDP EFISLVRKLL DLMNISYVIA CGEGEAQCVW
210 220 230 240 250
LQVSGAVDFI LSNDSDTLVF GGEKILKNYS KFYDDFGPSS ITSHSPSRHH
260 270 280 290 300
DSKESFVTVI DLPKINKVAG KKFDRLSLLF FSVLLGADYN RGVKGLGKNK
310 320 330 340 350
SLQLAQCEDP NFSMEFYDIF KDFNLEDLTS ESLRKSRYRL FQKRLYLYCK
360 370 380 390 400
DHSVELFGRN YPVLLNQGSF EGWPSTVAIM HYFHPIVQPY FDEEVLSDKY
410 420 430 440 450
INMAGNGHYR NLNFNELKYF LQSLNLPQIS SFDKWFHDSM HEMFLLREFL
460 470 480 490 500
SIDESDNIGK GNMRITEEKI MNIDGGKFQI PCFKIRYTTF LPNIPISSQS
510 520 530 540 550
PLKRSNSPSR SKSPTRRQMD IMEHPNSLWL PKYLIPQSHP LVIQYYETQQ
560 570 580 590 600
LIQKEKEKKG KKSNKSRLPQ KNNLDEFLRK HTSPIKSIGK VGESRKEILE
610 620 630 640 650
PVRKRLFVDT DEDTSLEEIP APTRLTTVDE HSDNDDDSLI FVDEITNSQS
660 670 680 690 700
VLDSSPGKRI RDLTQDEQVD VWKDVIEISP IKKSRTTNAE KNPPESGLKS
710 720 730 740 750
RSSITINARL QGTKMLPPNL TAPRLEREHS SVLDQLVTDA QDTVDRFVAC

DSDSSSTIE
Length:759
Mass (Da):87,364
Last modified:July 27, 2011 - v2
Checksum:iCE1305A9F78CA300
GO

Sequence cautioni

The sequence AAA60376 differs from that shown. Reason: Frameshift at positions 63, 673 and 759.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti59A → P in AAB64576 (PubMed:9169868).Curated1
Sequence conflicti164 – 165LL → FV in AAA60376 (Ref. 1) Curated2
Sequence conflicti346Y → C in AAA60376 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13615 Genomic DNA Translation: AAA60376.1 Frameshift.
U18796 Genomic DNA Translation: AAB64576.1
BK006939 Genomic DNA Translation: DAA07695.2
PIRiS50544
RefSeqiNP_010959.2, NM_001178932.2

Genome annotation databases

EnsemblFungiiYER041W; YER041W; YER041W
GeneIDi856764
KEGGisce:YER041W

Similar proteinsi

Entry informationi

Entry nameiYEN1_YEAST
AccessioniPrimary (citable) accession number: P40028
Secondary accession number(s): D3DLU1, Q02463
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 27, 2011
Last modified: April 25, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health