P40024 (ARB1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ABC transporter ATP-binding protein ARB1

Alternative name(s):
ATP-binding cassette protein involved in ribosome biogenesis 1

Gene names

Name:	ARB1
Ordered Locus Names:	YER036C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	610 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Stimulates 40S and 60S ribosome biogenesis. Ref.4
Subunit structure	Interacts with LSG1. Ref.4
Subcellular location	Cytoplasm. Nucleus. Note: Shuttles between the cytoplasm and the nucleus. Ref.4
Miscellaneous	Present with 17600 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily. Contains 2 ABC transporter domains.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ribosomal small subunit export from nucleus Inferred from mutant phenotype Ref.4. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 14562095. Source: SGD nucleus Inferred from direct assay Ref.4. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity, uncoupled Inferred from mutant phenotype Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 610	610	ABC transporter ATP-binding protein ARB1		PRO_0000093463
Regions
	Domain	82 – 323	242	ABC transporter 1
	Domain	393 – 610	218	ABC transporter 2
	Nucleotide binding	114 – 121	8	ATP 1 Potential
	Nucleotide binding	428 – 435	8	ATP 2 Potential
Amino acid modifications
	Modified residue	43	1	Phosphoserine Ref.5
	Modified residue	65	1	Phosphoserine Ref.5
	Modified residue	196	1	Phosphoserine Ref.5
	Modified residue	446	1	Phosphothreonine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P40024 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 0E5DB4A33059B4F0	FASTA	610	68,377
	10 20 30 40 50 60 MPPVSASKAK RDAKKAEREA KKAAAGKTIR KLGRKKEAAA EESEVDAAAR EIKMMKLQQD 70 80 90 100 110 120 KDGLSDRVVT GVLSSLETSR DIKLSSVSLL FHGKVLIQDS GLELNYGRRY GLLGENGCGK 130 140 150 160 170 180 STFLKALATR EYPIPEHIDI YLLDEPAEPS ELSALDYVVT EAQHELKRIE DLVEKTILED 190 200 210 220 230 240 GPESELLEPL YERMDSLDPD TFESRAAIIL IGLGFNKKTI LKKTKDMSGG WKMRVALAKA 250 260 270 280 290 300 LFVKPTLLLL DDPTAHLDLE ACVWLEEYLK RFDRTLVLVS HSQDFLNGVC TNMIDMRAQK 310 320 330 340 350 360 LTAYGGNYDS YHKTRSELET NQMKQYNKQQ EEIQHIKKFI ASAGTYANLV KQAKSRQKIL 370 380 390 400 410 420 DKMEADGLVQ PVVPDKVFSF RFPQVERLPP PVLAFDDISF HYESNPSENL YEHLNFGVDM 430 440 450 460 470 480 DSRIALVGPN GVGKSTLLKI MTGELTPQSG RVSRHTHVKL GVYSQHSQDQ LDLTKSALEF 490 500 510 520 530 540 VRDKYSNISQ DFQFWRGQLG RYGLTGEGQT VQMATLSEGQ RSRVVFALLA LEQPNVLLLD 550 560 570 580 590 600 EPTNGLDIPT IDSLADAINE FNGGVVVVSH DFRLLDKIAQ DIFVVENKTA TRWDGSILQY 610 KNKLAKNVVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"The novel ATP-binding cassette protein ARB1 is a shuttling factor that stimulates 40S and 60S ribosome biogenesis." Dong J., Lai R., Jennings J.L., Link A.J., Hinnebusch A.G. Mol. Cell. Biol. 25:9859-9873(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH LSG1.
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43; SER-65; SER-196 AND THR-446, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18796 Genomic DNA. Translation: AAB64571.1. BK006939 Genomic DNA. Translation: DAA07689.1.
PIR	S50539.
RefSeq	NP_010953.1. NM_001178927.1.
3D structure databases
ProteinModelPortal	P40024.
SMR	P40024. Positions 82-603.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4341N.
IntAct	P40024. 25 interactions.
MINT	MINT-508979.
STRING	4932.YER036C.
Proteomic databases
PaxDb	P40024.
PeptideAtlas	P40024.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER036C; YER036C; YER036C.
GeneID	856758.
KEGG	sce:YER036C.
Organism-specific databases
CYGD	YER036c.
SGD	S000000838. ARB1.
Phylogenomic databases
eggNOG	COG0488.
GeneTree	ENSGT00630000089910.
HOGENOM	HOG000271637.
KO	K06185.
OMA	VYSQHSA.
OrthoDB	EOG4MD17M.
Gene expression databases
Genevestigator	P40024.
GermOnline	YER036C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. [Graphical view]
Pfam	PF00005. ABC_tran. 2 hits. [Graphical view]
SMART	SM00382. AAA. 2 hits. [Graphical view]
PROSITE	PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	982923.

Entry information

Entry name

ARB1_YEAST

Accession

Primary (citable) accession number: P40024
Secondary accession number(s): D3DLT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program