P40020 (FIR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Factor interacting with REF2

Alternative name(s):
PAP1-interacting protein
Polymerase-interacting protein 1

Gene names

Name:	FIR1
Synonyms:	PIP1
Ordered Locus Names:	YER032W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	876 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in 3' mRNA processing. Positively regulates poly(A) synthesis. Ref.4 Ref.8
Subunit structure	Interacts with PAP1, REF2 and PBP1. Ref.1 Ref.4 Ref.8
Subcellular location	Bud neck Ref.6.
Post-translational modification	Phosphorylated by CDC28. Ref.7
Sequence caution	The sequence AAB46625.1 differs from that shown. Reason: Erroneous initiation. The sequence AAB64565.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	mRNA processing
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA polyadenylation Inferred from mutant phenotype Ref.4. Source: SGD
Cellular_component	cellular bud neck Inferred from direct assay Ref.6. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 876	876	Factor interacting with REF2		PRO_0000058446
Regions
	Compositional bias	655 – 683	29	Ser-rich
Natural variations
	Natural variant	614	1	P → R in strain: ATCC 200060 / W303. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P40020 [UniParc]. Last modified July 25, 2006. Version 3. Checksum: DFD2AB3684EBC1D2	FASTA	876	98,812
	10 20 30 40 50 60 MSLPVTPVKS KVCSTLSIQH EMDHDQCRDV LCPRENLLAI NRTNNIESVA IPRQRSSKNK 70 80 90 100 110 120 KPHEHTQSQV RFSIPDPNEI SQDSPLKIVF PKSGNETERR MSTSSLLMNS HGHLVDMHSK 130 140 150 160 170 180 ILVDVPKEVW KFHHNRRKKC ESRHRKTRSD VRSNTSSSGK EPNHSRSKSL QSIIVDTMNT 190 200 210 220 230 240 YRATEVETSI NENTSNISQV SPLNLSFDRP PPLTPEKNLY LTPESPLNRY HLPVPLEISL 250 260 270 280 290 300 PPYLSPQNKD KKRSSLVYDG DGYSQFQEGN TSSSTESSLE QPSSSYSDED DSIPYAHHDV 310 320 330 340 350 360 SFELNNADAD KLLGIDENAN VNLKIQRRNL KNPQHIKSKT DRECEEKNTE KNVSLKILST 370 380 390 400 410 420 PNKLIDIPDL EHMKSPPSTG LNGTLKFFQQ FEPSEEPTSP TRQVNPESLD KLDMSFKFPS 430 440 450 460 470 480 STTNNNVDKV HENRNSGDTN NEDFLKVDTS PVNQSFESRR QMLIDLQKSP TNNNPRIHKH 490 500 510 520 530 540 RRSRSVHNID DISLNFEATS TPPAPTSAPS IPVEHSNPCT SIEIPKRSPL RFTSSPKTSD 550 560 570 580 590 600 IPPEAQSPKN GSFLQEISVP SIQIIPDESI SHTREPSPSL IECPEDENEA FSTEVADHSI 610 620 630 640 650 660 AIISETKSVP SIEPFKPLSS FNSFGQEIQN KEPTPLNQTP TDLIGKQRNC VNPHSIPFSV 670 680 690 700 710 720 LSSNSQSSQS GSSKSSYNSE FSSNTAITDT TSQPSVTINR STLEHNFIEE KRSIKNLGHG 730 740 750 760 770 780 PPSQKNNYSF PRNKNTPSNR HDLDFNTIYE KRDGKMVEVI LLDEDEDVGL KNNDISRTRV 790 800 810 820 830 840 CHAQKAKNEQ QKKRLSHCNE ILGMCDKTAD DAKRIIYQLV NEKHKFSEKG QQTKPKKSRV 850 860 870 LPPLPFPLYD EKGNSLIPNK YQSSIHNDIP SHRKLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Uba2 and Ufd1 proteins of Saccharomyces cerevisiae interact with poly(A) polymerase and affect the polyadenylation activity of cell extracts." del Olmo M., Mizrahi N., Gross S., Moore C.L. Mol. Gen. Genet. 255:209-218(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INTERACTION WITH PAP1, VARIANT ARG-614. Strain: ATCC 200060 / W303.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"RNA binding analysis of yeast REF2 and its two-hybrid interaction with a new gene product, FIR1." Russnak R., Pereira S., Platt T. Gene Expr. 6:241-258(1996) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH REF2.
[5]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Targets of the cyclin-dependent kinase Cdk1." Ubersax J.A., Woodbury E.L., Quang P.N., Paraz M., Blethrow J.D., Shah K., Shokat K.M., Morgan D.O. Nature 425:859-864(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION BY CDC28.
[8]	"Identification of factors regulating poly(A) tail synthesis and maturation." Mangus D.A., Smith M.M., McSweeney J.M., Jacobson A. Mol. Cell. Biol. 24:4196-4206(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH PBP1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U17262 Genomic DNA. Translation: AAB46625.1. Different initiation. U18778 Genomic DNA. Translation: AAB64565.1. Different initiation. BK006939 Genomic DNA. Translation: DAA07685.1.
PIR	S50490.
RefSeq	NP_010949.4. NM_001178923.3.
3D structure databases
ProteinModelPortal	P40020.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-844N.
IntAct	P40020. 7 interactions.
MINT	MINT-486334.
STRING	4932.YER032W.
Proteomic databases
PRIDE	P40020.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER032W; YER032W; YER032W.
GeneID	856754.
KEGG	sce:YER032W. sce:YER035W.
Organism-specific databases
CYGD	YER032w.
SGD	S000000834. FIR1.
Phylogenomic databases
eggNOG	NOG87822.
OMA	GIDEDAN.
OrthoDB	EOG41CB5R.
Gene expression databases
Genevestigator	P40020.
GermOnline	YER032W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	982911.

Entry information

Entry name

FIR1_YEAST

Accession

Primary (citable) accession number: P40020
Secondary accession number(s): D3DLT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	July 25, 2006
Last modified:	April 3, 2013
This is version 83 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program