P40017 (YAT2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carnitine O-acetyltransferase YAT2
EC=2.3.1.7

Gene names

Name:	YAT2
Ordered Locus Names:	YER024W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	923 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the shutteling of acetyl-CoA in the cell. Ref.3
Catalytic activity	Acetyl-CoA + carnitine = CoA + O-acetylcarnitine.
Subcellular location	Cytoplasm Ref.4 Ref.7.
Miscellaneous	Present with 319 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the carnitine/choline acetyltransferase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism Transport
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	alcohol metabolic process Inferred from mutant phenotype Ref.3. Source: SGD carnitine metabolic process Inferred from mutant phenotype Ref.3. Source: SGD fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytosol Inferred from direct assay Ref.7. Source: SGD
Molecular_function	carnitine O-acetyltransferase activity Inferred from mutant phenotype Ref.3. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 923	923	Carnitine O-acetyltransferase YAT2		PRO_0000210176
Regions
	Region	530 – 541	12	Coenzyme A binding By similarity
Sites
	Binding site	576	1	Carnitine By similarity
Amino acid modifications
	Modified residue	782	1	Phosphothreonine Ref.6
	Modified residue	784	1	Phosphoserine Ref.8
	Modified residue	786	1	Phosphoserine Ref.6 Ref.8
	Modified residue	833	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P40017 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: B59AB881491D68A7	FASTA	923	103,334
	10 20 30 40 50 60 MSSGSTIVSS DKSGRTFKHE EELPKLPLPK LCDTLQRLKE SLEPLYYADG YYQHPLDPEQ 70 80 90 100 110 120 IEKLSSIIRD FEENPVSEKL QSKLQSYHDT RDCYLDELHL DINNQTSTRE IQDDVLPRNP 130 140 150 160 170 180 FLVLADDALP NITQADRSAV LVHSAARFIS ALKQDLLPPD INATNGKPLS MAPFLNLFGT 190 200 210 220 230 240 TRSPVFQRGE VENFDLNKPY TASDLEDPDY SSDEDDNDEP TQKDFDDRKR KHEEDIFTGN 250 260 270 280 290 300 GITIKRHPDS KHILIISRGQ YYTLEVLDST NKIIYTAAEL TTIFNHIIKD SSGIEKSTAL 310 320 330 340 350 360 GSLTSHSFRN WKYARKRLQK RYPNELHRID SALFVLVLDE SQEETTNDGD DTADISQMFN 370 380 390 400 410 420 RTITERDKKC TSANCKRVFY GTSIINSKGH QVGSCVSRWY DKLQLVVTAD AKATVIWDSF 430 440 450 460 470 480 TCDGSVVLRF TSEIYTESVL RLARDVNAGD PQFSLWPNVT QMDPETKKLM TATISADGGG 490 500 510 520 530 540 PSEIDPKLVV NKIDWSFSNI LNTHVHLSET KLADLISKYD IVRASIPLGR RSAQRLGVKP 550 560 570 580 590 600 DSMVQVALQI AHYALYGRMV FGLEPVSTRG FKNSRSSFIN IQSQALLELC QLFISSSIDG 610 620 630 640 650 660 TDKLDKFIQT CETHNNMVKH AKSGVGYEKH FNALKYLFKF HDHFGIHLSG DESSAAKDLF 670 680 690 700 710 720 ENPLVLPFSQ PELIVANCGN AATTTFGITP AVPHGFGIGY IIKDDQVDLT VTSQFRQGDR 730 740 750 760 770 780 LMFMLSWVLG EIRSYWRMSR GTSHNKTGVK ISPVVDKLYE MDNAVNNPPK RNGHTVNGSR 790 800 810 820 830 840 KTSSSSQVNL NRYGGFFDLE GHIDSRNISK TPSMKNLQKT FNGLTMSADN DHSSSAVSVP 850 860 870 880 890 900 TEKEKLNTGH EILQIQPREV ASNGLEADDE TDIEIVAGNA DGTSSSASSA TSLNSKKRNV 910 920 INSRFDIDFD RSRVGRKVAT LDQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Carnitine-dependent metabolic activities in Saccharomyces cerevisiae: three carnitine acetyltransferases are essential in a carnitine-dependent strain." Swiegers J.H., Dippenaar N., Pretorius I.S., Bauer F.F. Yeast 18:585-595(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-782 AND SER-786, MASS SPECTROMETRY.
[7]	"Carnitine and carnitine acetyltransferases in the yeast Saccharomyces cerevisiae: a role for carnitine in stress protection." Franken J., Kroppenstedt S., Swiegers J.H., Bauer F.F. Curr. Genet. 53:347-360(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-784; SER-786 AND SER-833, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18778 Genomic DNA. Translation: AAB64557.1. BK006939 Genomic DNA. Translation: DAA07677.1.
PIR	S50482.
RefSeq	NP_010941.1. NM_001178915.1.
3D structure databases
ProteinModelPortal	P40017.
SMR	P40017. Positions 15-713.
ModBase	Search...
Protein-protein interaction databases
IntAct	P40017. 1 interaction.
STRING	4932.YER024W.
Proteomic databases
PaxDb	P40017.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER024W; YER024W; YER024W.
GeneID	856745.
KEGG	sce:YER024W.
Organism-specific databases
CYGD	YER024w.
SGD	S000000826. YAT2.
Phylogenomic databases
eggNOG	NOG308143.
HOGENOM	HOG000141908.
OMA	DILPRNP.
OrthoDB	EOG4G7G6H.
Gene expression databases
Genevestigator	P40017.
GermOnline	YER024W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000542. Carn_acyl_trans. [Graphical view]
PANTHER	PTHR22589. PTHR22589. 1 hit.
Pfam	PF00755. Carn_acyltransf. 2 hits. [Graphical view]
PROSITE	PS00439. ACYLTRANSF_C_1. 1 hit. PS00440. ACYLTRANSF_C_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982885.

Entry information

Entry name

YAT2_YEAST

Accession

Primary (citable) accession number: P40017
Secondary accession number(s): D3DLS3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program