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P40016

- RPN3_YEAST

UniProt

P40016 - RPN3_YEAST

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Protein

26S proteasome regulatory subunit RPN3

Gene

RPN3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.

GO - Molecular functioni

  1. enzyme regulator activity Source: InterPro

GO - Biological processi

  1. regulation of protein catabolic process Source: InterPro
  2. ubiquitin-dependent protein catabolic process Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-30205-MONOMER.
ReactomeiREACT_100522. Regulation of activated PAK-2p34 by proteasome mediated degradation.
REACT_189025. ER-Phagosome pathway.
REACT_189047. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_189060. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_189250. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_230653. SCF-beta-TrCP mediated degradation of Emi1.
REACT_245870. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_245912. CDK-mediated phosphorylation and removal of Cdc6.
REACT_268441. Ubiquitin-dependent degradation of Cyclin D1.
REACT_83036. Orc1 removal from chromatin.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome regulatory subunit RPN3
Gene namesi
Name:RPN3
Synonyms:SUN2
Ordered Locus Names:YER021W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

SGDiS000000823. RPN3.

Subcellular locationi

GO - Cellular componenti

  1. proteasome regulatory particle, lid subcomplex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 52352226S proteasome regulatory subunit RPN3PRO_0000173827Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei454 – 4541Phosphoserine1 Publication

Post-translational modificationi

N-acetylated by NAT1.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP40016.
PaxDbiP40016.
PeptideAtlasiP40016.

Expressioni

Gene expression databases

GenevestigatoriP40016.

Interactioni

Subunit structurei

The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
RPN7Q061038EBI-15927,EBI-15940

Protein-protein interaction databases

BioGridi36755. 115 interactions.
DIPiDIP-1322N.
IntActiP40016. 32 interactions.
MINTiMINT-401279.
STRINGi4932.YER021W.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J47electron microscopy-S455-478[»]
4CR2electron microscopy7.70S1-523[»]
4CR3electron microscopy9.30S1-523[»]
4CR4electron microscopy8.80S1-523[»]
ProteinModelPortaliP40016.
SMRiP40016. Positions 126-478.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini343 – 447105PCIAdd
BLAST

Sequence similaritiesi

Belongs to the proteasome subunit S3 family.Curated
Contains 1 PCI domain.Curated

Phylogenomic databases

eggNOGiNOG251646.
GeneTreeiENSGT00490000043406.
HOGENOMiHOG000193909.
InParanoidiP40016.
KOiK03033.
OMAiEAKINHE.
OrthoDBiEOG7PVX27.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR013586. 26S_Psome_reg_C.
IPR013143. PAM.
IPR000717. PCI_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01399. PCI. 1 hit.
PF08375. Rpn3_C. 1 hit.
[Graphical view]
SMARTiSM00753. PAM. 1 hit.
SM00088. PINT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40016-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASTAVMMDV DSSGVNDLHH SEKKYAEEDQ VQELLKVLNE ISKTTLTLDP
60 70 80 90 100
RYIWRSLKDL SSLRNQELLN AETLCFTVNV LYPDSSSFKK NLLKFITSNH
110 120 130 140 150
KSSVPGSAEL RNSYPASFYS VNTEKKTIEV TAEINCFMHL LVQLFLWDSK
160 170 180 190 200
ELEQLVEFNR KVVIPNLLCY YNLRSLNLIN AKLWFYIYLS HETLARSSEE
210 220 230 240 250
INSDNQNIIL RSTMMKFLKI ASLKHDNETK AMLINLILRD FLNNGEVDSA
260 270 280 290 300
SDFISKLEYP HTDVSSSLEA RYFFYLSKIN AIQLDYSTAN EYIIAAIRKA
310 320 330 340 350
PHNSKSLGFL QQSNKLHCCI QLLMGDIPEL SFFHQSNMQK SLLPYYHLTK
360 370 380 390 400
AVKLGDLKKF TSTITKYKQL LLKDDTYQLC VRLRSNVIKT GIRIISLTYK
410 420 430 440 450
KISLRDICLK LNLDSEQTVE YMVSRAIRDG VIEAKINHED GFIETTELLN
460 470 480 490 500
IYDSEDPQQV FDERIKFANQ LHDEYLVSMR YPEDKKTQQN EKSENGENDD
510 520
DTLDGDLMDD MSDISDLDDL GFL
Length:523
Mass (Da):60,393
Last modified:October 5, 2010 - v5
Checksum:iB08B58663DDA85DC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti355 – 3551G → S no nucleotide entry (PubMed:8668124)Curated
Sequence conflicti355 – 3551G → S in BAA11208. (PubMed:9017604)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78023 Genomic DNA. Translation: BAA11208.1.
U18778 Genomic DNA. Translation: AAB64554.1.
BK006939 Genomic DNA. Translation: DAA07674.1.
PIRiS50479.
RefSeqiNP_010938.1. NM_001178912.1.

Genome annotation databases

EnsemblFungiiYER021W; YER021W; YER021W.
GeneIDi856742.
KEGGisce:YER021W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78023 Genomic DNA. Translation: BAA11208.1 .
U18778 Genomic DNA. Translation: AAB64554.1 .
BK006939 Genomic DNA. Translation: DAA07674.1 .
PIRi S50479.
RefSeqi NP_010938.1. NM_001178912.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3J47 electron microscopy - S 455-478 [» ]
4CR2 electron microscopy 7.70 S 1-523 [» ]
4CR3 electron microscopy 9.30 S 1-523 [» ]
4CR4 electron microscopy 8.80 S 1-523 [» ]
ProteinModelPortali P40016.
SMRi P40016. Positions 126-478.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36755. 115 interactions.
DIPi DIP-1322N.
IntActi P40016. 32 interactions.
MINTi MINT-401279.
STRINGi 4932.YER021W.

Proteomic databases

MaxQBi P40016.
PaxDbi P40016.
PeptideAtlasi P40016.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER021W ; YER021W ; YER021W .
GeneIDi 856742.
KEGGi sce:YER021W.

Organism-specific databases

SGDi S000000823. RPN3.

Phylogenomic databases

eggNOGi NOG251646.
GeneTreei ENSGT00490000043406.
HOGENOMi HOG000193909.
InParanoidi P40016.
KOi K03033.
OMAi EAKINHE.
OrthoDBi EOG7PVX27.

Enzyme and pathway databases

BioCyci YEAST:G3O-30205-MONOMER.
Reactomei REACT_100522. Regulation of activated PAK-2p34 by proteasome mediated degradation.
REACT_189025. ER-Phagosome pathway.
REACT_189047. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_189060. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_189250. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_230653. SCF-beta-TrCP mediated degradation of Emi1.
REACT_245870. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_245912. CDK-mediated phosphorylation and removal of Cdc6.
REACT_268441. Ubiquitin-dependent degradation of Cyclin D1.
REACT_83036. Orc1 removal from chromatin.

Miscellaneous databases

NextBioi 982876.
PROi P40016.

Gene expression databases

Genevestigatori P40016.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR013586. 26S_Psome_reg_C.
IPR013143. PAM.
IPR000717. PCI_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF01399. PCI. 1 hit.
PF08375. Rpn3_C. 1 hit.
[Graphical view ]
SMARTi SM00753. PAM. 1 hit.
SM00088. PINT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A multicopy suppressor of nin1-1 of the yeast Saccharomyces cerevisiae is a counterpart of the Drosophila melanogaster diphenol oxidase A2 gene, Dox-A2."
    Kawamura M., Kominami K., Takeuchi J., Toh-e A.
    Mol. Gen. Genet. 251:146-152(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Yeast counterparts of subunits S5a and p58 (S3) of the human 26S proteasome are encoded by two multicopy suppressors of nin1-1."
    Kominami K., Okura N., Kawamura M., Demartino G.N., Slaughter C.A., Shimbara N., Chung C.H., Fujimuro M., Yokosawa H., Shimizu Y., Tanahashi N., Tanaka K., Toh-e A.
    Mol. Biol. Cell 8:171-187(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "N-terminal modifications of the 19S regulatory particle subunits of the yeast proteasome."
    Kimura Y., Saeki Y., Yokosawa H., Polevoda B., Sherman F., Hirano H.
    Arch. Biochem. Biophys. 409:341-348(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-8, ACETYLATION AT ALA-2.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-454, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (7.4 ANGSTROMS) OF THE 26S PROTEASOME.

Entry informationi

Entry nameiRPN3_YEAST
AccessioniPrimary (citable) accession number: P40016
Secondary accession number(s): D3DLS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 5, 2010
Last modified: November 26, 2014
This is version 116 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 16700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3