26S proteasome regulatory subunit RPN3 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P40016 (RPN3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 100. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
26S proteasome regulatory subunit RPN3

Gene names

Name:	RPN3
Synonyms:	SUN2
Ordered Locus Names:	YER021W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	523 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
Subunit structure	The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively By similarity.
Post-translational modification	N-acetylated by NAT1.
Miscellaneous	Present with 16700 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the proteasome subunit S3 family. Contains 1 PCI domain.

Ontologies

Keywords
Cellular component	Proteasome
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	regulation of catalytic activity Inferred from electronic annotation. Source: GOC regulation of protein catabolic process Inferred from electronic annotation. Source: InterPro ubiquitin-dependent protein catabolic process Traceable author statement PubMed 8982460 PubMed 9697412. Source: SGD
Cellular_component	proteasome regulatory particle, lid subcomplex Inferred from direct assay PubMed 9741626. Source: SGD
Molecular_function	enzyme regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RPN7	Q06103	8	EBI-15927,EBI-15940

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.5

Chain

2 – 523

522

26S proteasome regulatory subunit RPN3

PRO_0000173827

Regions

Domain

343 – 447

105

PCI

Amino acid modifications

Modified residue

N-acetylalanine Ref.5

Modified residue

454

Phosphoserine Ref.7

Experimental info

Sequence conflict

355

G → S no nucleotide entry Ref.1

Sequence conflict

355

G → S in BAA11208. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P40016 [UniParc].

Last modified October 5, 2010. Version 5.
Checksum: B08B58663DDA85DC
Show »

FASTA

523

60,393

        10         20         30         40         50         60 
MASTAVMMDV DSSGVNDLHH SEKKYAEEDQ VQELLKVLNE ISKTTLTLDP RYIWRSLKDL 

        70         80         90        100        110        120 
SSLRNQELLN AETLCFTVNV LYPDSSSFKK NLLKFITSNH KSSVPGSAEL RNSYPASFYS 

       130        140        150        160        170        180 
VNTEKKTIEV TAEINCFMHL LVQLFLWDSK ELEQLVEFNR KVVIPNLLCY YNLRSLNLIN 

       190        200        210        220        230        240 
AKLWFYIYLS HETLARSSEE INSDNQNIIL RSTMMKFLKI ASLKHDNETK AMLINLILRD 

       250        260        270        280        290        300 
FLNNGEVDSA SDFISKLEYP HTDVSSSLEA RYFFYLSKIN AIQLDYSTAN EYIIAAIRKA 

       310        320        330        340        350        360 
PHNSKSLGFL QQSNKLHCCI QLLMGDIPEL SFFHQSNMQK SLLPYYHLTK AVKLGDLKKF 

       370        380        390        400        410        420 
TSTITKYKQL LLKDDTYQLC VRLRSNVIKT GIRIISLTYK KISLRDICLK LNLDSEQTVE 

       430        440        450        460        470        480 
YMVSRAIRDG VIEAKINHED GFIETTELLN IYDSEDPQQV FDERIKFANQ LHDEYLVSMR 

       490        500        510        520 
YPEDKKTQQN EKSENGENDD DTLDGDLMDD MSDISDLDDL GFL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A multicopy suppressor of nin1-1 of the yeast Saccharomyces cerevisiae is a counterpart of the Drosophila melanogaster diphenol oxidase A2 gene, Dox-A2." Kawamura M., Kominami K., Takeuchi J., Toh-e A. Mol. Gen. Genet. 251:146-152(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Yeast counterparts of subunits S5a and p58 (S3) of the human 26S proteasome are encoded by two multicopy suppressors of nin1-1." Kominami K., Okura N., Kawamura M., Demartino G.N., Slaughter C.A., Shimbara N., Chung C.H., Fujimuro M., Yokosawa H., Shimizu Y., Tanahashi N., Tanaka K., Toh-e A. Mol. Biol. Cell 8:171-187(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"N-terminal modifications of the 19S regulatory particle subunits of the yeast proteasome." Kimura Y., Saeki Y., Yokosawa H., Polevoda B., Sherman F., Hirano H. Arch. Biochem. Biophys. 409:341-348(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-8, ACETYLATION AT ALA-2.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-454, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D78023 Genomic DNA. Translation: BAA11208.1.
U18778 Genomic DNA. Translation: AAB64554.1.
BK006939 Genomic DNA. Translation: DAA07674.1.

PIR

S50479.

RefSeq

NP_010938.1. NM_001178912.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4B4T	electron microscopy	7.40	S	1-523	[»]

ProteinModelPortal

P40016.

SMR

P40016. Positions 203-451.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1322N.

IntAct

P40016. 35 interactions.

MINT

MINT-401279.

STRING

4932.YER021W.

Proteomic databases

PaxDb

P40016.

PeptideAtlas

P40016.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YER021W; YER021W; YER021W.

GeneID

856742.

KEGG

sce:YER021W.

Organism-specific databases

SGD

S000000823. RPN3.

Phylogenomic databases

eggNOG

NOG251646.

GeneTree

ENSGT00490000043406.

HOGENOM

HOG000193909.

K03033.

OMA

LKHDNET.

OrthoDB

EOG4350FR.

Gene expression databases

Genevestigator

P40016.

GermOnline

YER021W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

1.10.10.10. 1 hit.

InterPro

IPR013586. 26S_Psome_reg_C.
IPR013143. PAM.
IPR000717. PCI_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]

Pfam

PF01399. PCI. 1 hit.
PF08375. Rpn3_C. 1 hit.
[Graphical view]

SMART

SM00753. PAM. 1 hit.
SM00088. PINT. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

982876.

Entry information

Entry name

RPN3_YEAST

Accession

Primary (citable) accession number: P40016
Secondary accession number(s): D3DLS0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	October 5, 2010
Last modified:	May 1, 2013
This is version 100 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents