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P40016

- RPN3_YEAST

UniProt

P40016 - RPN3_YEAST

Protein

26S proteasome regulatory subunit RPN3

Gene

RPN3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 5 (05 Oct 2010)
      Previous versions | rss
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    Functioni

    Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.

    GO - Molecular functioni

    1. enzyme regulator activity Source: InterPro
    2. protein binding Source: IntAct

    GO - Biological processi

    1. regulation of protein catabolic process Source: InterPro
    2. ubiquitin-dependent protein catabolic process Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30205-MONOMER.
    ReactomeiREACT_100522. Regulation of activated PAK-2p34 by proteasome mediated degradation.
    REACT_189025. ER-Phagosome pathway.
    REACT_189047. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_189060. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_189250. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_83036. Orc1 removal from chromatin.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    26S proteasome regulatory subunit RPN3
    Gene namesi
    Name:RPN3
    Synonyms:SUN2
    Ordered Locus Names:YER021W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    SGDiS000000823. RPN3.

    Subcellular locationi

    GO - Cellular componenti

    1. proteasome regulatory particle, lid subcomplex Source: SGD

    Keywords - Cellular componenti

    Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 52352226S proteasome regulatory subunit RPN3PRO_0000173827Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication
    Modified residuei454 – 4541Phosphoserine1 Publication

    Post-translational modificationi

    N-acetylated by NAT1.1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP40016.
    PaxDbiP40016.
    PeptideAtlasiP40016.

    Expressioni

    Gene expression databases

    GenevestigatoriP40016.

    Interactioni

    Subunit structurei

    The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RPN7Q061038EBI-15927,EBI-15940

    Protein-protein interaction databases

    BioGridi36755. 114 interactions.
    DIPiDIP-1322N.
    IntActiP40016. 32 interactions.
    MINTiMINT-401279.
    STRINGi4932.YER021W.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3J47electron microscopy-S455-478[»]
    4CR2electron microscopy7.70S1-523[»]
    4CR3electron microscopy9.30S1-523[»]
    4CR4electron microscopy8.80S1-523[»]
    ProteinModelPortaliP40016.
    SMRiP40016. Positions 126-478.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini343 – 447105PCIAdd
    BLAST

    Sequence similaritiesi

    Belongs to the proteasome subunit S3 family.Curated
    Contains 1 PCI domain.Curated

    Phylogenomic databases

    eggNOGiNOG251646.
    GeneTreeiENSGT00490000043406.
    HOGENOMiHOG000193909.
    KOiK03033.
    OMAiEAKINHE.
    OrthoDBiEOG7PVX27.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR013586. 26S_Psome_reg_C.
    IPR013143. PAM.
    IPR000717. PCI_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF01399. PCI. 1 hit.
    PF08375. Rpn3_C. 1 hit.
    [Graphical view]
    SMARTiSM00753. PAM. 1 hit.
    SM00088. PINT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P40016-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASTAVMMDV DSSGVNDLHH SEKKYAEEDQ VQELLKVLNE ISKTTLTLDP    50
    RYIWRSLKDL SSLRNQELLN AETLCFTVNV LYPDSSSFKK NLLKFITSNH 100
    KSSVPGSAEL RNSYPASFYS VNTEKKTIEV TAEINCFMHL LVQLFLWDSK 150
    ELEQLVEFNR KVVIPNLLCY YNLRSLNLIN AKLWFYIYLS HETLARSSEE 200
    INSDNQNIIL RSTMMKFLKI ASLKHDNETK AMLINLILRD FLNNGEVDSA 250
    SDFISKLEYP HTDVSSSLEA RYFFYLSKIN AIQLDYSTAN EYIIAAIRKA 300
    PHNSKSLGFL QQSNKLHCCI QLLMGDIPEL SFFHQSNMQK SLLPYYHLTK 350
    AVKLGDLKKF TSTITKYKQL LLKDDTYQLC VRLRSNVIKT GIRIISLTYK 400
    KISLRDICLK LNLDSEQTVE YMVSRAIRDG VIEAKINHED GFIETTELLN 450
    IYDSEDPQQV FDERIKFANQ LHDEYLVSMR YPEDKKTQQN EKSENGENDD 500
    DTLDGDLMDD MSDISDLDDL GFL 523
    Length:523
    Mass (Da):60,393
    Last modified:October 5, 2010 - v5
    Checksum:iB08B58663DDA85DC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti355 – 3551G → S no nucleotide entry (PubMed:8668124)Curated
    Sequence conflicti355 – 3551G → S in BAA11208. (PubMed:9017604)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D78023 Genomic DNA. Translation: BAA11208.1.
    U18778 Genomic DNA. Translation: AAB64554.1.
    BK006939 Genomic DNA. Translation: DAA07674.1.
    PIRiS50479.
    RefSeqiNP_010938.1. NM_001178912.1.

    Genome annotation databases

    EnsemblFungiiYER021W; YER021W; YER021W.
    GeneIDi856742.
    KEGGisce:YER021W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D78023 Genomic DNA. Translation: BAA11208.1 .
    U18778 Genomic DNA. Translation: AAB64554.1 .
    BK006939 Genomic DNA. Translation: DAA07674.1 .
    PIRi S50479.
    RefSeqi NP_010938.1. NM_001178912.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3J47 electron microscopy - S 455-478 [» ]
    4CR2 electron microscopy 7.70 S 1-523 [» ]
    4CR3 electron microscopy 9.30 S 1-523 [» ]
    4CR4 electron microscopy 8.80 S 1-523 [» ]
    ProteinModelPortali P40016.
    SMRi P40016. Positions 126-478.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36755. 114 interactions.
    DIPi DIP-1322N.
    IntActi P40016. 32 interactions.
    MINTi MINT-401279.
    STRINGi 4932.YER021W.

    Proteomic databases

    MaxQBi P40016.
    PaxDbi P40016.
    PeptideAtlasi P40016.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER021W ; YER021W ; YER021W .
    GeneIDi 856742.
    KEGGi sce:YER021W.

    Organism-specific databases

    SGDi S000000823. RPN3.

    Phylogenomic databases

    eggNOGi NOG251646.
    GeneTreei ENSGT00490000043406.
    HOGENOMi HOG000193909.
    KOi K03033.
    OMAi EAKINHE.
    OrthoDBi EOG7PVX27.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30205-MONOMER.
    Reactomei REACT_100522. Regulation of activated PAK-2p34 by proteasome mediated degradation.
    REACT_189025. ER-Phagosome pathway.
    REACT_189047. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_189060. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_189250. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_83036. Orc1 removal from chromatin.

    Miscellaneous databases

    NextBioi 982876.
    PROi P40016.

    Gene expression databases

    Genevestigatori P40016.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR013586. 26S_Psome_reg_C.
    IPR013143. PAM.
    IPR000717. PCI_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF01399. PCI. 1 hit.
    PF08375. Rpn3_C. 1 hit.
    [Graphical view ]
    SMARTi SM00753. PAM. 1 hit.
    SM00088. PINT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A multicopy suppressor of nin1-1 of the yeast Saccharomyces cerevisiae is a counterpart of the Drosophila melanogaster diphenol oxidase A2 gene, Dox-A2."
      Kawamura M., Kominami K., Takeuchi J., Toh-e A.
      Mol. Gen. Genet. 251:146-152(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Yeast counterparts of subunits S5a and p58 (S3) of the human 26S proteasome are encoded by two multicopy suppressors of nin1-1."
      Kominami K., Okura N., Kawamura M., Demartino G.N., Slaughter C.A., Shimbara N., Chung C.H., Fujimuro M., Yokosawa H., Shimizu Y., Tanahashi N., Tanaka K., Toh-e A.
      Mol. Biol. Cell 8:171-187(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "N-terminal modifications of the 19S regulatory particle subunits of the yeast proteasome."
      Kimura Y., Saeki Y., Yokosawa H., Polevoda B., Sherman F., Hirano H.
      Arch. Biochem. Biophys. 409:341-348(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-8, ACETYLATION AT ALA-2.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-454, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (7.4 ANGSTROMS) OF THE 26S PROTEASOME.

    Entry informationi

    Entry nameiRPN3_YEAST
    AccessioniPrimary (citable) accession number: P40016
    Secondary accession number(s): D3DLS0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: October 5, 2010
    Last modified: October 1, 2014
    This is version 114 of the entry and version 5 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 16700 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3