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P40015

- ISC1_YEAST

UniProt

P40015 - ISC1_YEAST

Protein

Inositol phosphosphingolipids phospholipase C

Gene

ISC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Responsible for the hydrolysis of the phosphosphingolipids (IPS), inositol phosphorylceramide (IPC), mannosylinositol phosphorylceramide (MIPC), and mannosyldiinositol phosphorylceramide (M(IP)2C). Also active on sphingomyelin, but this activity is probably not physiologically relevant.

    Cofactori

    Magnesium.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi100 – 1001MagnesiumBy similarity
    Sitei233 – 2331Important for substrate recognitionBy similarity
    Active sitei334 – 3341Proton acceptorBy similarity

    GO - Molecular functioni

    1. inositol phosphosphingolipid phospholipase activity Source: SGD
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. ceramide biosynthetic process Source: SGD
    2. response to salt stress Source: SGD
    3. sphingolipid catabolic process Source: SGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid metabolism, Sphingolipid metabolism

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-17704.
    YEAST:YER019W-MONOMER.
    ReactomeiREACT_188975. Glycosphingolipid metabolism.
    UniPathwayiUPA00222.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inositol phosphosphingolipids phospholipase C (EC:3.1.4.-)
    Short name:
    IPS phospholipase C
    Short name:
    IPS-PLC
    Alternative name(s):
    Neutral sphingomyelinase
    Short name:
    N-SMase
    Short name:
    nSMase
    Gene namesi
    Name:ISC1
    Ordered Locus Names:YER019W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYER019w.
    SGDiS000000821. ISC1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: SGD
    2. integral component of mitochondrial outer membrane Source: SGD
    3. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 477477Inositol phosphosphingolipids phospholipase CPRO_0000075695Add
    BLAST

    Proteomic databases

    MaxQBiP40015.
    PaxDbiP40015.
    PeptideAtlasiP40015.

    Expressioni

    Gene expression databases

    GenevestigatoriP40015.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    AGP1P253761EBI-9371,EBI-2357
    PMP2P409751EBI-9371,EBI-2043041

    Protein-protein interaction databases

    BioGridi36752. 172 interactions.
    DIPiDIP-5002N.
    IntActiP40015. 3 interactions.
    MINTiMINT-486977.
    STRINGi4932.YER019W.

    Structurei

    3D structure databases

    ProteinModelPortaliP40015.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 398398CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini418 – 4247ExtracellularSequence Analysis
    Topological domaini450 – 47728CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei399 – 41719HelicalSequence AnalysisAdd
    BLAST
    Transmembranei425 – 44925HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the neutral sphingomyelinase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG269293.
    GeneTreeiENSGT00390000009166.
    HOGENOMiHOG000215232.
    KOiK01128.
    OMAiNSHMHAP.
    OrthoDBiEOG7J70QP.

    Family and domain databases

    Gene3Di3.60.10.10. 1 hit.
    InterProiIPR005135. Endo/exonuclease/phosphatase.
    [Graphical view]
    PfamiPF03372. Exo_endo_phos. 1 hit.
    [Graphical view]
    SUPFAMiSSF56219. SSF56219. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P40015-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYNRKDRDVH ERKEDGQSEF EALNGTNAIM SDNSKAYSIK FLTFNTWGLK    50
    YVSKHRKERL RAIADKLAGH SMLTPISDEL LPNGGDSNEN EDYDVIALQE 100
    IWCVEDWKYL ASACASKYPY QRLFHSGILT GPGLAILSKV PIESTFLYRF 150
    PINGRPSAVF RGDWYVGKSI AITVLNTGTR PIAIMNSHMH APYAKQGDAA 200
    YLCHRSCQAW DFSRLIKLYR QAGYAVIVVG DLNSRPGSLP HKFLTQEAGL 250
    VDSWEQLHGK QDLAVIARLS PLQQLLKGCT TCDSLLNTWR AQRQPDEACR 300
    LDYALIDPDF LQTVDAGVRF TERIPHLDCS VSDHFAYSCT LNIVPQGTES 350
    RPSTSVKRAK THDRELILQR YSNYETMIEC IHTYLKTAQR QKFFRGLHFW 400
    ASILLLIASL VVTTFTANKA GWSSIFWVLF AIAVSISGTI DGAISFLFGR 450
    SEIRALIEVE QEVLDAEHHL QTFLSEK 477
    Length:477
    Mass (Da):53,940
    Last modified:February 1, 1995 - v1
    Checksum:i0670FD303FEB8EFF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18778 Genomic DNA. Translation: AAB64552.1.
    BK006939 Genomic DNA. Translation: DAA07671.1.
    PIRiS50477.
    RefSeqiNP_010935.1. NM_001178910.1.

    Genome annotation databases

    EnsemblFungiiYER019W; YER019W; YER019W.
    GeneIDi856739.
    KEGGisce:YER019W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18778 Genomic DNA. Translation: AAB64552.1 .
    BK006939 Genomic DNA. Translation: DAA07671.1 .
    PIRi S50477.
    RefSeqi NP_010935.1. NM_001178910.1.

    3D structure databases

    ProteinModelPortali P40015.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36752. 172 interactions.
    DIPi DIP-5002N.
    IntActi P40015. 3 interactions.
    MINTi MINT-486977.
    STRINGi 4932.YER019W.

    Proteomic databases

    MaxQBi P40015.
    PaxDbi P40015.
    PeptideAtlasi P40015.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER019W ; YER019W ; YER019W .
    GeneIDi 856739.
    KEGGi sce:YER019W.

    Organism-specific databases

    CYGDi YER019w.
    SGDi S000000821. ISC1.

    Phylogenomic databases

    eggNOGi NOG269293.
    GeneTreei ENSGT00390000009166.
    HOGENOMi HOG000215232.
    KOi K01128.
    OMAi NSHMHAP.
    OrthoDBi EOG7J70QP.

    Enzyme and pathway databases

    UniPathwayi UPA00222 .
    BioCyci MetaCyc:MONOMER-17704.
    YEAST:YER019W-MONOMER.
    Reactomei REACT_188975. Glycosphingolipid metabolism.

    Miscellaneous databases

    NextBioi 982867.
    PROi P40015.

    Gene expression databases

    Genevestigatori P40015.

    Family and domain databases

    Gene3Di 3.60.10.10. 1 hit.
    InterProi IPR005135. Endo/exonuclease/phosphatase.
    [Graphical view ]
    Pfami PF03372. Exo_endo_phos. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56219. SSF56219. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Identification of ISC1 (YER019w) as inositol phosphosphingolipid phospholipase C in Saccharomyces cerevisiae."
      Sawai H., Okamoto Y., Luberto C., Mao C., Bielawska A., Domae N., Hannun Y.A.
      J. Biol. Chem. 275:39793-39798(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.

    Entry informationi

    Entry nameiISC1_YEAST
    AccessioniPrimary (citable) accession number: P40015
    Secondary accession number(s): D3DLR7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2170 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3