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Protein

Inositol phosphosphingolipids phospholipase C

Gene

ISC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the hydrolysis of the phosphosphingolipids (IPS), inositol phosphorylceramide (IPC), mannosylinositol phosphorylceramide (MIPC), and mannosyldiinositol phosphorylceramide (M(IP)2C). Also active on sphingomyelin, but this activity is probably not physiologically relevant.

Cofactori

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi100 – 1001MagnesiumBy similarity
Sitei233 – 2331Important for substrate recognitionBy similarity
Active sitei334 – 3341Proton acceptorBy similarity

GO - Molecular functioni

  1. inositol phosphosphingolipid phospholipase activity Source: SGD
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. ceramide biosynthetic process Source: SGD
  2. response to salt stress Source: SGD
  3. sphingolipid catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17704.
YEAST:YER019W-MONOMER.
ReactomeiREACT_188975. Glycosphingolipid metabolism.
REACT_230803. Ceramide signalling.
UniPathwayiUPA00222.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol phosphosphingolipids phospholipase C (EC:3.1.4.-)
Short name:
IPS phospholipase C
Short name:
IPS-PLC
Alternative name(s):
Neutral sphingomyelinase
Short name:
N-SMase
Short name:
nSMase
Gene namesi
Name:ISC1
Ordered Locus Names:YER019W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER019w.
SGDiS000000821. ISC1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 398398CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei399 – 41719HelicalSequence AnalysisAdd
BLAST
Topological domaini418 – 4247ExtracellularSequence Analysis
Transmembranei425 – 44925HelicalSequence AnalysisAdd
BLAST
Topological domaini450 – 47728CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: SGD
  2. integral component of mitochondrial outer membrane Source: SGD
  3. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 477477Inositol phosphosphingolipids phospholipase CPRO_0000075695Add
BLAST

Proteomic databases

MaxQBiP40015.
PaxDbiP40015.
PeptideAtlasiP40015.

Expressioni

Gene expression databases

GenevestigatoriP40015.

Interactioni

Protein-protein interaction databases

BioGridi36752. 173 interactions.
DIPiDIP-5002N.
IntActiP40015. 3 interactions.
MINTiMINT-486977.
STRINGi4932.YER019W.

Structurei

3D structure databases

ProteinModelPortaliP40015.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the neutral sphingomyelinase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG269293.
GeneTreeiENSGT00390000009166.
HOGENOMiHOG000215232.
InParanoidiP40015.
KOiK01128.
OMAiNSHMHAP.
OrthoDBiEOG7J70QP.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PfamiPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.

Sequencei

Sequence statusi: Complete.

P40015-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYNRKDRDVH ERKEDGQSEF EALNGTNAIM SDNSKAYSIK FLTFNTWGLK
60 70 80 90 100
YVSKHRKERL RAIADKLAGH SMLTPISDEL LPNGGDSNEN EDYDVIALQE
110 120 130 140 150
IWCVEDWKYL ASACASKYPY QRLFHSGILT GPGLAILSKV PIESTFLYRF
160 170 180 190 200
PINGRPSAVF RGDWYVGKSI AITVLNTGTR PIAIMNSHMH APYAKQGDAA
210 220 230 240 250
YLCHRSCQAW DFSRLIKLYR QAGYAVIVVG DLNSRPGSLP HKFLTQEAGL
260 270 280 290 300
VDSWEQLHGK QDLAVIARLS PLQQLLKGCT TCDSLLNTWR AQRQPDEACR
310 320 330 340 350
LDYALIDPDF LQTVDAGVRF TERIPHLDCS VSDHFAYSCT LNIVPQGTES
360 370 380 390 400
RPSTSVKRAK THDRELILQR YSNYETMIEC IHTYLKTAQR QKFFRGLHFW
410 420 430 440 450
ASILLLIASL VVTTFTANKA GWSSIFWVLF AIAVSISGTI DGAISFLFGR
460 470
SEIRALIEVE QEVLDAEHHL QTFLSEK
Length:477
Mass (Da):53,940
Last modified:February 1, 1995 - v1
Checksum:i0670FD303FEB8EFF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18778 Genomic DNA. Translation: AAB64552.1.
BK006939 Genomic DNA. Translation: DAA07671.1.
PIRiS50477.
RefSeqiNP_010935.1. NM_001178910.1.

Genome annotation databases

EnsemblFungiiYER019W; YER019W; YER019W.
GeneIDi856739.
KEGGisce:YER019W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18778 Genomic DNA. Translation: AAB64552.1.
BK006939 Genomic DNA. Translation: DAA07671.1.
PIRiS50477.
RefSeqiNP_010935.1. NM_001178910.1.

3D structure databases

ProteinModelPortaliP40015.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36752. 173 interactions.
DIPiDIP-5002N.
IntActiP40015. 3 interactions.
MINTiMINT-486977.
STRINGi4932.YER019W.

Proteomic databases

MaxQBiP40015.
PaxDbiP40015.
PeptideAtlasiP40015.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER019W; YER019W; YER019W.
GeneIDi856739.
KEGGisce:YER019W.

Organism-specific databases

CYGDiYER019w.
SGDiS000000821. ISC1.

Phylogenomic databases

eggNOGiNOG269293.
GeneTreeiENSGT00390000009166.
HOGENOMiHOG000215232.
InParanoidiP40015.
KOiK01128.
OMAiNSHMHAP.
OrthoDBiEOG7J70QP.

Enzyme and pathway databases

UniPathwayiUPA00222.
BioCyciMetaCyc:MONOMER-17704.
YEAST:YER019W-MONOMER.
ReactomeiREACT_188975. Glycosphingolipid metabolism.
REACT_230803. Ceramide signalling.

Miscellaneous databases

NextBioi982867.
PROiP40015.

Gene expression databases

GenevestigatoriP40015.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PfamiPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Identification of ISC1 (YER019w) as inositol phosphosphingolipid phospholipase C in Saccharomyces cerevisiae."
    Sawai H., Okamoto Y., Luberto C., Mao C., Bielawska A., Domae N., Hannun Y.A.
    J. Biol. Chem. 275:39793-39798(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiISC1_YEAST
AccessioniPrimary (citable) accession number: P40015
Secondary accession number(s): D3DLR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 4, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2170 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.