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Protein

Inositol phosphosphingolipids phospholipase C

Gene

ISC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Responsible for the hydrolysis of the phosphosphingolipids (IPS), inositol phosphorylceramide (IPC), mannosylinositol phosphorylceramide (MIPC), and mannosyldiinositol phosphorylceramide (M(IP)2C). Also active on sphingomyelin, but this activity is probably not physiologically relevant.

Miscellaneous

Present with 2170 molecules/cell in log phase SD medium.1 Publication

Cofactori

Pathwayi: sphingolipid metabolism

This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi100MagnesiumBy similarity1
Sitei233Important for substrate recognitionBy similarity1
Active sitei334Proton acceptorBy similarity1

GO - Molecular functioni

  • inositol phosphosphingolipid phospholipase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • ceramide biosynthetic process Source: SGD
  • response to salt stress Source: SGD
  • sphingolipid catabolic process Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processLipid metabolism, Sphingolipid metabolism
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:YER019W-MONOMER
YEAST:YER019W-MONOMER
ReactomeiR-SCE-1660662 Glycosphingolipid metabolism
UniPathwayiUPA00222

Chemistry databases

SwissLipidsiSLP:000001859

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol phosphosphingolipids phospholipase C (EC:3.1.4.-)
Short name:
IPS phospholipase C
Short name:
IPS-PLC
Alternative name(s):
Neutral sphingomyelinase
Short name:
N-SMase
Short name:
nSMase
Gene namesi
Name:ISC1
Ordered Locus Names:YER019W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER019W
SGDiS000000821 ISC1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 398CytoplasmicSequence analysisAdd BLAST398
Transmembranei399 – 417HelicalSequence analysisAdd BLAST19
Topological domaini418 – 424ExtracellularSequence analysis7
Transmembranei425 – 449HelicalSequence analysisAdd BLAST25
Topological domaini450 – 477CytoplasmicSequence analysisAdd BLAST28

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000756951 – 477Inositol phosphosphingolipids phospholipase CAdd BLAST477

Proteomic databases

MaxQBiP40015
PaxDbiP40015
PRIDEiP40015
TopDownProteomicsiP40015

Interactioni

Protein-protein interaction databases

BioGridi36752, 245 interactors
DIPiDIP-5002N
IntActiP40015, 8 interactors
STRINGi4932.YER019W

Structurei

3D structure databases

ProteinModelPortaliP40015
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the neutral sphingomyelinase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000009166
HOGENOMiHOG000215232
InParanoidiP40015
KOiK12351
OMAiNSHMHAP
OrthoDBiEOG092C3CH8

Family and domain databases

Gene3Di3.60.10.10, 1 hit
InterProiView protein in InterPro
IPR036691 Endo/exonu/phosph_ase_sf
IPR005135 Endo/exonuclease/phosphatase
PfamiView protein in Pfam
PF03372 Exo_endo_phos, 1 hit
SUPFAMiSSF56219 SSF56219, 1 hit

Sequencei

Sequence statusi: Complete.

P40015-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYNRKDRDVH ERKEDGQSEF EALNGTNAIM SDNSKAYSIK FLTFNTWGLK
60 70 80 90 100
YVSKHRKERL RAIADKLAGH SMLTPISDEL LPNGGDSNEN EDYDVIALQE
110 120 130 140 150
IWCVEDWKYL ASACASKYPY QRLFHSGILT GPGLAILSKV PIESTFLYRF
160 170 180 190 200
PINGRPSAVF RGDWYVGKSI AITVLNTGTR PIAIMNSHMH APYAKQGDAA
210 220 230 240 250
YLCHRSCQAW DFSRLIKLYR QAGYAVIVVG DLNSRPGSLP HKFLTQEAGL
260 270 280 290 300
VDSWEQLHGK QDLAVIARLS PLQQLLKGCT TCDSLLNTWR AQRQPDEACR
310 320 330 340 350
LDYALIDPDF LQTVDAGVRF TERIPHLDCS VSDHFAYSCT LNIVPQGTES
360 370 380 390 400
RPSTSVKRAK THDRELILQR YSNYETMIEC IHTYLKTAQR QKFFRGLHFW
410 420 430 440 450
ASILLLIASL VVTTFTANKA GWSSIFWVLF AIAVSISGTI DGAISFLFGR
460 470
SEIRALIEVE QEVLDAEHHL QTFLSEK
Length:477
Mass (Da):53,940
Last modified:February 1, 1995 - v1
Checksum:i0670FD303FEB8EFF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18778 Genomic DNA Translation: AAB64552.1
BK006939 Genomic DNA Translation: DAA07671.1
PIRiS50477
RefSeqiNP_010935.1, NM_001178910.1

Genome annotation databases

EnsemblFungiiYER019W; YER019W; YER019W
GeneIDi856739
KEGGisce:YER019W

Similar proteinsi

Entry informationi

Entry nameiISC1_YEAST
AccessioniPrimary (citable) accession number: P40015
Secondary accession number(s): D3DLR7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 28, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health