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P40014

- SPC25_YEAST

UniProt

P40014 - SPC25_YEAST

Protein

Kinetochore protein SPC25

Gene

SPC25

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.2 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. structural constituent of cytoskeleton Source: SGD

    GO - Biological processi

    1. chromosome segregation Source: SGD
    2. microtubule nucleation Source: SGD
    3. mitotic nuclear division Source: UniProtKB-KW

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30203-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kinetochore protein SPC25
    Gene namesi
    Name:SPC25
    Ordered Locus Names:YER018C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYER018c.
    SGDiS000000820. SPC25.

    Subcellular locationi

    Nucleus. Chromosomecentromerekinetochore
    Note: Associated with kinetochores.

    GO - Cellular componenti

    1. condensed nuclear chromosome, centromeric region Source: SGD
    2. condensed nuclear chromosome kinetochore Source: SGD
    3. Ndc80 complex Source: SGD

    Keywords - Cellular componenti

    Centromere, Chromosome, Kinetochore, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 221220Kinetochore protein SPC25PRO_0000202622Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP40014.
    PaxDbiP40014.

    Expressioni

    Gene expression databases

    GenevestigatoriP40014.

    Interactioni

    Subunit structurei

    Component of the NDC80 complex, which consists of TID3/NDC80, NUF2, SPC24 and SPC25. The NDC80 complex is formed by two subcomplexes, TID3/NDC80-NUF2 and SPC24-SPC25, which are joined end-to-end through their coiled-coil domains. It has a rod-like structure with a length of 570 Angstroms and globular domains at either end. The TID3/NDC80-NUF2 globular domains are probably directed to microtubules, the SPC24-SPC25 globular domains to the centromere.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    NUF2P338956EBI-22458,EBI-12377
    SPC24Q0447720EBI-22458,EBI-27228
    TID3P4046013EBI-22458,EBI-25247

    Protein-protein interaction databases

    BioGridi36751. 113 interactions.
    DIPiDIP-2990N.
    IntActiP40014. 13 interactions.
    MINTiMINT-437184.
    STRINGi4932.YER018C.

    Structurei

    Secondary structure

    1
    221
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi134 – 14512
    Beta strandi147 – 1515
    Beta strandi153 – 1575
    Beta strandi159 – 17315
    Beta strandi175 – 1784
    Beta strandi181 – 1866
    Helixi190 – 20112
    Helixi206 – 21914

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2FTXX-ray1.90A133-221[»]
    2FV4NMR-A128-221[»]
    4GEQX-ray2.01A/C133-221[»]
    ProteinModelPortaliP40014.
    SMRiP40014. Positions 133-221.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP40014.

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili9 – 112104Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the SPC25 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG123509.
    KOiK11558.
    OMAiYHERESE.
    OrthoDBiEOG7VDZ1J.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P40014-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASIDAFSDL ERRMDGFQKD VAQVLARQQN HARQQLQQFQ AEMRQLHNQH    50
    QHLIDELQRL ATQRTALQQQ IHAAQQATNT TREQWRSYHE RESELSRRQS 100
    TLAAQSRELD SLLQQRGKEC VQLRARWAAQ SGNDAAEVAL YERLLQLRVL 150
    PGASDVHDVR FVFGDDSRCW IEVAMHGDHV IGNSHPALDP KSRATLEHVL 200
    TVQGDLAAFL VVARDMLLAS L 221
    Length:221
    Mass (Da):25,244
    Last modified:February 1, 1995 - v1
    Checksum:i1BED6CCFF330A5D8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18778 Genomic DNA. Translation: AAB64551.1.
    X81066 Genomic DNA. Translation: CAA56952.1.
    BK006939 Genomic DNA. Translation: DAA07670.1.
    PIRiS50476.
    RefSeqiNP_010934.3. NM_001178909.3.

    Genome annotation databases

    EnsemblFungiiYER018C; YER018C; YER018C.
    GeneIDi856738.
    KEGGisce:YER018C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18778 Genomic DNA. Translation: AAB64551.1 .
    X81066 Genomic DNA. Translation: CAA56952.1 .
    BK006939 Genomic DNA. Translation: DAA07670.1 .
    PIRi S50476.
    RefSeqi NP_010934.3. NM_001178909.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2FTX X-ray 1.90 A 133-221 [» ]
    2FV4 NMR - A 128-221 [» ]
    4GEQ X-ray 2.01 A/C 133-221 [» ]
    ProteinModelPortali P40014.
    SMRi P40014. Positions 133-221.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36751. 113 interactions.
    DIPi DIP-2990N.
    IntActi P40014. 13 interactions.
    MINTi MINT-437184.
    STRINGi 4932.YER018C.

    Proteomic databases

    MaxQBi P40014.
    PaxDbi P40014.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER018C ; YER018C ; YER018C .
    GeneIDi 856738.
    KEGGi sce:YER018C.

    Organism-specific databases

    CYGDi YER018c.
    SGDi S000000820. SPC25.

    Phylogenomic databases

    eggNOGi NOG123509.
    KOi K11558.
    OMAi YHERESE.
    OrthoDBi EOG7VDZ1J.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30203-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P40014.
    NextBioi 982864.

    Gene expression databases

    Genevestigatori P40014.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Identification of a set of yeast genes coding for a novel family of putative ATPases with high similarity to constituents of the 26S protease complex."
      Schnall R., Mannhaupt G., Stucka R., Tauer R., Ehnle S., Schwarzlose C., Vetter I., Feldmann H.
      Yeast 10:1141-1155(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 67-221.
      Strain: ATCC 204508 / S288c.
    4. "The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control."
      Janke C., Ortiz J., Lechner J., Shevchenko A., Shevchenko A., Magiera M.M., Schramm C., Schiebel E.
      EMBO J. 20:777-791(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH TID3 AND NUF2.
    5. "Spc24 interacts with Mps2 and is required for chromosome segregation, but is not implicated in spindle pole body duplication."
      Le Masson I., Saveanu C., Chevalier A., Namane A., Gobin R., Fromont-Racine M., Jacquier A., Mann C.
      Mol. Microbiol. 43:1431-1443(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NDC80 AND SPC24.
    6. "The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation."
      Wigge P.A., Kilmartin J.V.
      J. Cell Biol. 152:349-360(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE NDC80 COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION IN THE NDC80 COMPLEX.
    7. "The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity."
      McCleland M.L., Gardner R.D., Kallio M.J., Daum J.R., Gorbsky G.J., Burke D.J., Stukenberg P.T.
      Genes Dev. 17:101-114(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE NDC80 COMPLEX.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "The fission yeast kinetochore component Spc7 associates with the EB1 family member Mal3 and is required for kinetochore-spindle association."
      Kerres A., Vietmeier-Decker C., Ortiz J., Karig I., Beuter C., Hegemann J., Lechner J., Fleig U.
      Mol. Biol. Cell 15:5255-5267(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE NDC80 COMPLEX.
    10. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    11. "Molecular organization of the Ndc80 complex, an essential kinetochore component."
      Wei R.R., Sorger P.K., Harrison S.C.
      Proc. Natl. Acad. Sci. U.S.A. 102:5363-5367(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF THE NDC80 COMPLEX.

    Entry informationi

    Entry nameiSPC25_YEAST
    AccessioniPrimary (citable) accession number: P40014
    Secondary accession number(s): D3DLR6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3280 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3