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P40014 (SPC25_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kinetochore protein SPC25
Gene names
Name:SPC25
Ordered Locus Names:YER018C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length221 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity. Ref.6 Ref.7

Subunit structure

Component of the NDC80 complex, which consists of TID3/NDC80, NUF2, SPC24 and SPC25. The NDC80 complex is formed by two subcomplexes, TID3/NDC80-NUF2 and SPC24-SPC25, which are joined end-to-end through their coiled-coil domains. It has a rod-like structure with a length of 570 Angstroms and globular domains at either end. The TID3/NDC80-NUF2 globular domains are probably directed to microtubules, the SPC24-SPC25 globular domains to the centromere. Ref.6 Ref.9

Subcellular location

Nucleus. Chromosomecentromerekinetochore. Note: Associated with kinetochores. Ref.4 Ref.6

Miscellaneous

Present with 3280 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the SPC25 family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.10
Chain2 – 221220Kinetochore protein SPC25
PRO_0000202622

Regions

Coiled coil9 – 112104 Potential

Amino acid modifications

Modified residue21N-acetylalanine Ref.10

Secondary structure

................. 221
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P40014 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 1BED6CCFF330A5D8

FASTA22125,244
        10         20         30         40         50         60 
MASIDAFSDL ERRMDGFQKD VAQVLARQQN HARQQLQQFQ AEMRQLHNQH QHLIDELQRL 

        70         80         90        100        110        120 
ATQRTALQQQ IHAAQQATNT TREQWRSYHE RESELSRRQS TLAAQSRELD SLLQQRGKEC 

       130        140        150        160        170        180 
VQLRARWAAQ SGNDAAEVAL YERLLQLRVL PGASDVHDVR FVFGDDSRCW IEVAMHGDHV 

       190        200        210        220 
IGNSHPALDP KSRATLEHVL TVQGDLAAFL VVARDMLLAS L 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Identification of a set of yeast genes coding for a novel family of putative ATPases with high similarity to constituents of the 26S protease complex."
Schnall R., Mannhaupt G., Stucka R., Tauer R., Ehnle S., Schwarzlose C., Vetter I., Feldmann H.
Yeast 10:1141-1155(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 67-221.
Strain: ATCC 204508 / S288c.
[4]"The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control."
Janke C., Ortiz J., Lechner J., Shevchenko A., Shevchenko A., Magiera M.M., Schramm C., Schiebel E.
EMBO J. 20:777-791(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH TID3 AND NUF2.
[5]"Spc24 interacts with Mps2 and is required for chromosome segregation, but is not implicated in spindle pole body duplication."
Le Masson I., Saveanu C., Chevalier A., Namane A., Gobin R., Fromont-Racine M., Jacquier A., Mann C.
Mol. Microbiol. 43:1431-1443(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NDC80 AND SPC24.
[6]"The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation."
Wigge P.A., Kilmartin J.V.
J. Cell Biol. 152:349-360(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE NDC80 COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION IN THE NDC80 COMPLEX.
[7]"The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity."
McCleland M.L., Gardner R.D., Kallio M.J., Daum J.R., Gorbsky G.J., Burke D.J., Stukenberg P.T.
Genes Dev. 17:101-114(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE NDC80 COMPLEX.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"The fission yeast kinetochore component Spc7 associates with the EB1 family member Mal3 and is required for kinetochore-spindle association."
Kerres A., Vietmeier-Decker C., Ortiz J., Karig I., Beuter C., Hegemann J., Lechner J., Fleig U.
Mol. Biol. Cell 15:5255-5267(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE NDC80 COMPLEX.
[10]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[11]"Molecular organization of the Ndc80 complex, an essential kinetochore component."
Wei R.R., Sorger P.K., Harrison S.C.
Proc. Natl. Acad. Sci. U.S.A. 102:5363-5367(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING OF THE NDC80 COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U18778 Genomic DNA. Translation: AAB64551.1.
X81066 Genomic DNA. Translation: CAA56952.1.
BK006939 Genomic DNA. Translation: DAA07670.1.
PIRS50476.
RefSeqNP_010934.3. NM_001178909.3.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2FTXX-ray1.90A133-221[»]
2FV4NMR-A128-221[»]
4GEQX-ray2.01A/C133-221[»]
ProteinModelPortalP40014.
SMRP40014. Positions 133-221.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36751. 113 interactions.
DIPDIP-2990N.
IntActP40014. 13 interactions.
MINTMINT-437184.
STRING4932.YER018C.

Proteomic databases

PaxDbP40014.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYER018C; YER018C; YER018C.
GeneID856738.
KEGGsce:YER018C.

Organism-specific databases

CYGDYER018c.
SGDS000000820. SPC25.

Phylogenomic databases

eggNOGNOG123509.
KOK11558.
OMAYHERESE.
OrthoDBEOG7VDZ1J.

Enzyme and pathway databases

BioCycYEAST:G3O-30203-MONOMER.

Gene expression databases

GenevestigatorP40014.

Family and domain databases

ProtoNetSearch...

Other

EvolutionaryTraceP40014.
NextBio982864.

Entry information

Entry nameSPC25_YEAST
AccessionPrimary (citable) accession number: P40014
Secondary accession number(s): D3DLR6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references