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Protein

Kinetochore protein SPC25

Gene

SPC25

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.2 Publications

Miscellaneous

Present with 3280 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • structural constituent of cytoskeleton Source: SGD

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: SGD
  • microtubule nucleation Source: SGD

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-30203-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore protein SPC25
Gene namesi
Name:SPC25
Ordered Locus Names:YER018C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER018C
SGDiS000000820 SPC25

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002026222 – 221Kinetochore protein SPC25Add BLAST220

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP40014
PaxDbiP40014
PRIDEiP40014

PTM databases

iPTMnetiP40014

Interactioni

Subunit structurei

Component of the NDC80 complex, which consists of TID3/NDC80, NUF2, SPC24 and SPC25. The NDC80 complex is formed by two subcomplexes, TID3/NDC80-NUF2 and SPC24-SPC25, which are joined end-to-end through their coiled-coil domains. It has a rod-like structure with a length of 570 Angstroms and globular domains at either end. The TID3/NDC80-NUF2 globular domains are probably directed to microtubules, the SPC24-SPC25 globular domains to the centromere.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36751, 171 interactors
DIPiDIP-2990N
IntActiP40014, 22 interactors
MINTiP40014
STRINGi4932.YER018C

Structurei

Secondary structure

1221
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi133 – 145Combined sources13
Beta strandi147 – 151Combined sources5
Beta strandi153 – 155Combined sources3
Beta strandi158 – 164Combined sources7
Beta strandi169 – 173Combined sources5
Beta strandi175 – 178Combined sources4
Beta strandi180 – 186Combined sources7
Helixi190 – 201Combined sources12
Helixi206 – 220Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FTXX-ray1.90A133-221[»]
2FV4NMR-A128-221[»]
4GEQX-ray2.01A/C133-221[»]
5T6JX-ray1.75B133-221[»]
5TCSX-ray2.83D1-31[»]
D138-221[»]
5TD8X-ray7.53D1-221[»]
ProteinModelPortaliP40014
SMRiP40014
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40014

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili9 – 112Sequence analysisAdd BLAST104

Sequence similaritiesi

Belongs to the SPC25 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

InParanoidiP40014
KOiK11558
OMAiYHERESE
OrthoDBiEOG092C4Y0E

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40014-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASIDAFSDL ERRMDGFQKD VAQVLARQQN HARQQLQQFQ AEMRQLHNQH
60 70 80 90 100
QHLIDELQRL ATQRTALQQQ IHAAQQATNT TREQWRSYHE RESELSRRQS
110 120 130 140 150
TLAAQSRELD SLLQQRGKEC VQLRARWAAQ SGNDAAEVAL YERLLQLRVL
160 170 180 190 200
PGASDVHDVR FVFGDDSRCW IEVAMHGDHV IGNSHPALDP KSRATLEHVL
210 220
TVQGDLAAFL VVARDMLLAS L
Length:221
Mass (Da):25,244
Last modified:February 1, 1995 - v1
Checksum:i1BED6CCFF330A5D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18778 Genomic DNA Translation: AAB64551.1
X81066 Genomic DNA Translation: CAA56952.1
BK006939 Genomic DNA Translation: DAA07670.1
PIRiS50476
RefSeqiNP_010934.3, NM_001178909.3

Genome annotation databases

EnsemblFungiiYER018C; YER018C; YER018C
GeneIDi856738
KEGGisce:YER018C

Similar proteinsi

Entry informationi

Entry nameiSPC25_YEAST
AccessioniPrimary (citable) accession number: P40014
Secondary accession number(s): D3DLR6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 25, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health