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Protein

Kinetochore protein SPC25

Gene

SPC25

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.2 Publications

GO - Molecular functioni

  • structural constituent of cytoskeleton Source: SGD

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: SGD
  • microtubule nucleation Source: SGD
  • mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-30203-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore protein SPC25
Gene namesi
Name:SPC25
Ordered Locus Names:YER018C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome V

Organism-specific databases

CYGDiYER018c.
EuPathDBiFungiDB:YER018C.
SGDiS000000820. SPC25.

Subcellular locationi

GO - Cellular componenti

  • condensed nuclear chromosome, centromeric region Source: SGD
  • condensed nuclear chromosome kinetochore Source: SGD
  • Ndc80 complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 221220Kinetochore protein SPC25PRO_0000202622Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP40014.
PaxDbiP40014.

Interactioni

Subunit structurei

Component of the NDC80 complex, which consists of TID3/NDC80, NUF2, SPC24 and SPC25. The NDC80 complex is formed by two subcomplexes, TID3/NDC80-NUF2 and SPC24-SPC25, which are joined end-to-end through their coiled-coil domains. It has a rod-like structure with a length of 570 Angstroms and globular domains at either end. The TID3/NDC80-NUF2 globular domains are probably directed to microtubules, the SPC24-SPC25 globular domains to the centromere.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NDC80P4046013EBI-22458,EBI-25247
NUF2P338956EBI-22458,EBI-12377
SPC24Q0447720EBI-22458,EBI-27228

Protein-protein interaction databases

BioGridi36751. 114 interactions.
DIPiDIP-2990N.
IntActiP40014. 13 interactions.
MINTiMINT-437184.

Structurei

Secondary structure

1
221
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi134 – 14512Combined sources
Beta strandi147 – 1515Combined sources
Beta strandi153 – 1575Combined sources
Beta strandi159 – 17315Combined sources
Beta strandi175 – 1784Combined sources
Beta strandi181 – 1866Combined sources
Helixi190 – 20112Combined sources
Helixi206 – 21914Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FTXX-ray1.90A133-221[»]
2FV4NMR-A128-221[»]
4GEQX-ray2.01A/C133-221[»]
ProteinModelPortaliP40014.
SMRiP40014. Positions 133-221.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40014.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili9 – 112104Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the SPC25 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG123509.
InParanoidiP40014.
KOiK11558.
OMAiYHERESE.
OrthoDBiEOG7VDZ1J.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40014-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASIDAFSDL ERRMDGFQKD VAQVLARQQN HARQQLQQFQ AEMRQLHNQH
60 70 80 90 100
QHLIDELQRL ATQRTALQQQ IHAAQQATNT TREQWRSYHE RESELSRRQS
110 120 130 140 150
TLAAQSRELD SLLQQRGKEC VQLRARWAAQ SGNDAAEVAL YERLLQLRVL
160 170 180 190 200
PGASDVHDVR FVFGDDSRCW IEVAMHGDHV IGNSHPALDP KSRATLEHVL
210 220
TVQGDLAAFL VVARDMLLAS L
Length:221
Mass (Da):25,244
Last modified:February 1, 1995 - v1
Checksum:i1BED6CCFF330A5D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18778 Genomic DNA. Translation: AAB64551.1.
X81066 Genomic DNA. Translation: CAA56952.1.
BK006939 Genomic DNA. Translation: DAA07670.1.
PIRiS50476.
RefSeqiNP_010934.3. NM_001178909.3.

Genome annotation databases

EnsemblFungiiYER018C; YER018C; YER018C.
GeneIDi856738.
KEGGisce:YER018C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18778 Genomic DNA. Translation: AAB64551.1.
X81066 Genomic DNA. Translation: CAA56952.1.
BK006939 Genomic DNA. Translation: DAA07670.1.
PIRiS50476.
RefSeqiNP_010934.3. NM_001178909.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FTXX-ray1.90A133-221[»]
2FV4NMR-A128-221[»]
4GEQX-ray2.01A/C133-221[»]
ProteinModelPortaliP40014.
SMRiP40014. Positions 133-221.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36751. 114 interactions.
DIPiDIP-2990N.
IntActiP40014. 13 interactions.
MINTiMINT-437184.

Proteomic databases

MaxQBiP40014.
PaxDbiP40014.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER018C; YER018C; YER018C.
GeneIDi856738.
KEGGisce:YER018C.

Organism-specific databases

CYGDiYER018c.
EuPathDBiFungiDB:YER018C.
SGDiS000000820. SPC25.

Phylogenomic databases

eggNOGiNOG123509.
InParanoidiP40014.
KOiK11558.
OMAiYHERESE.
OrthoDBiEOG7VDZ1J.

Enzyme and pathway databases

BioCyciYEAST:G3O-30203-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP40014.
NextBioi982864.
PROiP40014.

Family and domain databases

ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Identification of a set of yeast genes coding for a novel family of putative ATPases with high similarity to constituents of the 26S protease complex."
    Schnall R., Mannhaupt G., Stucka R., Tauer R., Ehnle S., Schwarzlose C., Vetter I., Feldmann H.
    Yeast 10:1141-1155(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 67-221.
    Strain: ATCC 204508 / S288c.
  4. "The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control."
    Janke C., Ortiz J., Lechner J., Shevchenko A., Shevchenko A., Magiera M.M., Schramm C., Schiebel E.
    EMBO J. 20:777-791(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH TID3 AND NUF2.
  5. "Spc24 interacts with Mps2 and is required for chromosome segregation, but is not implicated in spindle pole body duplication."
    Le Masson I., Saveanu C., Chevalier A., Namane A., Gobin R., Fromont-Racine M., Jacquier A., Mann C.
    Mol. Microbiol. 43:1431-1443(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NDC80 AND SPC24.
  6. "The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation."
    Wigge P.A., Kilmartin J.V.
    J. Cell Biol. 152:349-360(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE NDC80 COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION IN THE NDC80 COMPLEX.
  7. "The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity."
    McCleland M.L., Gardner R.D., Kallio M.J., Daum J.R., Gorbsky G.J., Burke D.J., Stukenberg P.T.
    Genes Dev. 17:101-114(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE NDC80 COMPLEX.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "The fission yeast kinetochore component Spc7 associates with the EB1 family member Mal3 and is required for kinetochore-spindle association."
    Kerres A., Vietmeier-Decker C., Ortiz J., Karig I., Beuter C., Hegemann J., Lechner J., Fleig U.
    Mol. Biol. Cell 15:5255-5267(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE NDC80 COMPLEX.
  10. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  11. "Molecular organization of the Ndc80 complex, an essential kinetochore component."
    Wei R.R., Sorger P.K., Harrison S.C.
    Proc. Natl. Acad. Sci. U.S.A. 102:5363-5367(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF THE NDC80 COMPLEX.

Entry informationi

Entry nameiSPC25_YEAST
AccessioniPrimary (citable) accession number: P40014
Secondary accession number(s): D3DLR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 22, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3280 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.