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Protein

Kinetochore protein SPC25

Gene

SPC25

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.2 Publications

GO - Molecular functioni

  • structural constituent of cytoskeleton Source: SGD

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: SGD
  • microtubule nucleation Source: SGD
  • mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-30203-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore protein SPC25
Gene namesi
Name:SPC25
Ordered Locus Names:YER018C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER018C.
SGDiS000000820. SPC25.

Subcellular locationi

GO - Cellular componenti

  • condensed nuclear chromosome, centromeric region Source: SGD
  • condensed nuclear chromosome kinetochore Source: SGD
  • Ndc80 complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002026222 – 221Kinetochore protein SPC25Add BLAST220

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP40014.
PRIDEiP40014.

Interactioni

Subunit structurei

Component of the NDC80 complex, which consists of TID3/NDC80, NUF2, SPC24 and SPC25. The NDC80 complex is formed by two subcomplexes, TID3/NDC80-NUF2 and SPC24-SPC25, which are joined end-to-end through their coiled-coil domains. It has a rod-like structure with a length of 570 Angstroms and globular domains at either end. The TID3/NDC80-NUF2 globular domains are probably directed to microtubules, the SPC24-SPC25 globular domains to the centromere.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NDC80P4046013EBI-22458,EBI-25247
NUF2P338956EBI-22458,EBI-12377
SPC24Q0447720EBI-22458,EBI-27228

Protein-protein interaction databases

BioGridi36751. 115 interactors.
DIPiDIP-2990N.
IntActiP40014. 13 interactors.
MINTiMINT-437184.

Structurei

Secondary structure

1221
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi134 – 145Combined sources12
Beta strandi147 – 151Combined sources5
Beta strandi153 – 157Combined sources5
Beta strandi159 – 173Combined sources15
Beta strandi175 – 178Combined sources4
Beta strandi181 – 186Combined sources6
Helixi190 – 201Combined sources12
Helixi206 – 219Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FTXX-ray1.90A133-221[»]
2FV4NMR-A128-221[»]
4GEQX-ray2.01A/C133-221[»]
ProteinModelPortaliP40014.
SMRiP40014.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40014.

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili9 – 112Sequence analysisAdd BLAST104

Sequence similaritiesi

Belongs to the SPC25 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

InParanoidiP40014.
KOiK11558.
OMAiYHERESE.
OrthoDBiEOG092C4Y0E.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40014-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASIDAFSDL ERRMDGFQKD VAQVLARQQN HARQQLQQFQ AEMRQLHNQH
60 70 80 90 100
QHLIDELQRL ATQRTALQQQ IHAAQQATNT TREQWRSYHE RESELSRRQS
110 120 130 140 150
TLAAQSRELD SLLQQRGKEC VQLRARWAAQ SGNDAAEVAL YERLLQLRVL
160 170 180 190 200
PGASDVHDVR FVFGDDSRCW IEVAMHGDHV IGNSHPALDP KSRATLEHVL
210 220
TVQGDLAAFL VVARDMLLAS L
Length:221
Mass (Da):25,244
Last modified:February 1, 1995 - v1
Checksum:i1BED6CCFF330A5D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18778 Genomic DNA. Translation: AAB64551.1.
X81066 Genomic DNA. Translation: CAA56952.1.
BK006939 Genomic DNA. Translation: DAA07670.1.
PIRiS50476.
RefSeqiNP_010934.3. NM_001178909.3.

Genome annotation databases

EnsemblFungiiYER018C; YER018C; YER018C.
GeneIDi856738.
KEGGisce:YER018C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18778 Genomic DNA. Translation: AAB64551.1.
X81066 Genomic DNA. Translation: CAA56952.1.
BK006939 Genomic DNA. Translation: DAA07670.1.
PIRiS50476.
RefSeqiNP_010934.3. NM_001178909.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FTXX-ray1.90A133-221[»]
2FV4NMR-A128-221[»]
4GEQX-ray2.01A/C133-221[»]
ProteinModelPortaliP40014.
SMRiP40014.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36751. 115 interactors.
DIPiDIP-2990N.
IntActiP40014. 13 interactors.
MINTiMINT-437184.

Proteomic databases

MaxQBiP40014.
PRIDEiP40014.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER018C; YER018C; YER018C.
GeneIDi856738.
KEGGisce:YER018C.

Organism-specific databases

EuPathDBiFungiDB:YER018C.
SGDiS000000820. SPC25.

Phylogenomic databases

InParanoidiP40014.
KOiK11558.
OMAiYHERESE.
OrthoDBiEOG092C4Y0E.

Enzyme and pathway databases

BioCyciYEAST:G3O-30203-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP40014.
PROiP40014.

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiSPC25_YEAST
AccessioniPrimary (citable) accession number: P40014
Secondary accession number(s): D3DLR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3280 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.