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P40013 (BIM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein BIM1
Gene names
Name:BIM1
Ordered Locus Names:YER016W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length344 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds microtubules.

Subcellular location

Cytoplasmcytoskeleton.

Miscellaneous

Present with 3630 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the MAPRE family.

Contains 1 CH (calponin-homology) domain.

Contains 1 EB1 C-terminal domain.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Mitosis
   Cellular componentCytoplasm
Cytoskeleton
Microtubule
   PTMAcetylation
Phosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmicrotubule depolymerization

Inferred from mutant phenotype PubMed 10352017. Source: SGD

microtubule nucleation

Inferred from physical interaction PubMed 9153752. Source: SGD

mitotic sister chromatid cohesion

Inferred from mutant phenotype PubMed 14742714. Source: SGD

mitotic spindle assembly checkpoint

Traceable author statement PubMed 10935617. Source: SGD

negative regulation of microtubule depolymerization

Inferred from mutant phenotype PubMed 10352017. Source: SGD

nuclear migration along microtubule

Inferred from mutant phenotype PubMed 10352017. Source: SGD

positive regulation of microtubule polymerization

Inferred from direct assay PubMed 20392838. Source: SGD

   Cellular_componentcytoplasmic microtubule

Inferred from direct assay PubMed 10352017. Source: SGD

microtubule plus-end

Inferred from direct assay PubMed 20392838. Source: SGD

spindle

Inferred from direct assay PubMed 10352017. Source: SGD

spindle midzone

Inferred from direct assay PubMed 10352017. Source: SGD

spindle pole

Inferred from direct assay PubMed 10352017. Source: SGD

spindle pole body

Inferred from direct assay PubMed 9585415. Source: SGD

   Molecular_functionmicrotubule plus-end binding

Inferred from direct assay PubMed 20392838. Source: SGD

protein binding

Inferred from physical interaction PubMed 12925767. Source: IntAct

protein homodimerization activity

Inferred from direct assay PubMed 20392838. Source: SGD

structural constituent of cytoskeleton

Inferred from physical interaction PubMed 9585415. Source: SGD

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

DAM1P532672EBI-3614,EBI-23268

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.9
Chain2 – 344343Protein BIM1
PRO_0000213432

Regions

Domain8 – 107100CH
Domain188 – 28194EB1 C-terminal

Amino acid modifications

Modified residue21N-acetylserine Ref.9
Modified residue1571Phosphoserine Ref.6

Secondary structure

....................... 344
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P40013 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: C9087CB46A32BD74

FASTA34438,362
        10         20         30         40         50         60 
MSAGIGESRT ELLTWLNGLL NLNYKKIEEC GTGAAYCQIM DSIYGDLPMN RVKFNATAEY 

        70         80         90        100        110        120 
EFQTNYKILQ SCFSRHGIEK TVYVDKLIRC KFQDNLEFLQ WLKKHWIRHK DESVYDPDAR 

       130        140        150        160        170        180 
RKYRPIITNN SATKPRTVSN PTTAKRSSST GTGSAMSGGL ATRHSSLGIN GSRKTSVTQG 

       190        200        210        220        230        240 
QLVAIQAELT KSQETIGSLN EEIEQYKGTV STLEIEREFY FNKLRDIEIL VHTTQDLINE 

       250        260        270        280        290        300 
GVYKFNDETI TGHGNGNGGA LLRFVKKVES ILYATAEGFE MNDGEDELND KNLGEHGTVP 

       310        320        330        340 
NQGGYANSNG EVNGNEGSNH DVIMQNDEGE VGVSNNLIID EETF 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"BIM1 encodes a microtubule-binding protein in yeast."
Schwartz K., Richards K., Botstein D.
Mol. Biol. Cell 8:2677-2691(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[6]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-157, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U18778 Genomic DNA. Translation: AAB64549.1.
BK006939 Genomic DNA. Translation: DAA07668.1.
PIRS50474.
RefSeqNP_010932.1. NM_001178907.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2QJXX-ray1.90A1-124[»]
4E61X-ray2.45A/B182-282[»]
ProteinModelPortalP40013.
SMRP40013. Positions 8-124, 180-281.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36749. 461 interactions.
DIPDIP-1295N.
IntActP40013. 16 interactions.
MINTMINT-410426.
STRING4932.YER016W.

Proteomic databases

MaxQBP40013.
PaxDbP40013.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYER016W; YER016W; YER016W.
GeneID856736.
KEGGsce:YER016W.

Organism-specific databases

CYGDYER016w.
SGDS000000818. BIM1.

Phylogenomic databases

eggNOGCOG5217.
GeneTreeENSGT00490000043329.
HOGENOMHOG000198048.
KOK10436.
OMATKHEYEY.
OrthoDBEOG7HXD2Z.

Enzyme and pathway databases

BioCycYEAST:G3O-30201-MONOMER.

Gene expression databases

GenevestigatorP40013.

Family and domain databases

Gene3D1.10.418.10. 1 hit.
InterProIPR001715. CH-domain.
IPR004953. EB1_C.
IPR027328. MAPRE.
[Graphical view]
PANTHERPTHR10623. PTHR10623. 1 hit.
PfamPF00307. CH. 1 hit.
PF03271. EB1. 1 hit.
[Graphical view]
SUPFAMSSF140612. SSF140612. 1 hit.
SSF47576. SSF47576. 1 hit.
PROSITEPS51230. EB1_C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP40013.
NextBio982858.
PROP40013.

Entry information

Entry nameBIM1_YEAST
AccessionPrimary (citable) accession number: P40013
Secondary accession number(s): D3DLR4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references