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P40013

- BIM1_YEAST

UniProt

P40013 - BIM1_YEAST

Protein

Protein BIM1

Gene

BIM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Binds microtubules.

    GO - Molecular functioni

    1. microtubule plus-end binding Source: SGD
    2. protein binding Source: IntAct
    3. protein homodimerization activity Source: SGD
    4. structural constituent of cytoskeleton Source: SGD

    GO - Biological processi

    1. microtubule depolymerization Source: SGD
    2. microtubule nucleation Source: SGD
    3. mitotic sister chromatid cohesion Source: SGD
    4. mitotic spindle assembly checkpoint Source: SGD
    5. negative regulation of microtubule depolymerization Source: SGD
    6. nuclear migration along microtubule Source: SGD
    7. positive regulation of microtubule polymerization Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30201-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein BIM1
    Gene namesi
    Name:BIM1
    Ordered Locus Names:YER016W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYER016w.
    SGDiS000000818. BIM1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasmic microtubule Source: SGD
    2. microtubule plus-end Source: SGD
    3. spindle Source: SGD
    4. spindle midzone Source: SGD
    5. spindle pole Source: SGD
    6. spindle pole body Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Microtubule

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 344343Protein BIM1PRO_0000213432Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei157 – 1571Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP40013.
    PaxDbiP40013.

    Expressioni

    Gene expression databases

    GenevestigatoriP40013.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    DAM1P532672EBI-3614,EBI-23268

    Protein-protein interaction databases

    BioGridi36749. 461 interactions.
    DIPiDIP-1295N.
    IntActiP40013. 16 interactions.
    MINTiMINT-410426.
    STRINGi4932.YER016W.

    Structurei

    Secondary structure

    1
    344
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi9 – 2012
    Helixi27 – 326
    Helixi34 – 4411
    Helixi49 – 513
    Helixi59 – 7517
    Helixi84 – 874
    Turni88 – 903
    Helixi92 – 10918
    Helixi117 – 1204
    Turni121 – 1233
    Helixi182 – 23958
    Helixi260 – 27718

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2QJXX-ray1.90A1-124[»]
    4E61X-ray2.45A/B182-282[»]
    ProteinModelPortaliP40013.
    SMRiP40013. Positions 8-124, 180-281.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP40013.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini8 – 107100CHAdd
    BLAST
    Domaini188 – 28194EB1 C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the MAPRE family.Curated
    Contains 1 EB1 C-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5217.
    GeneTreeiENSGT00490000043329.
    HOGENOMiHOG000198048.
    KOiK10436.
    OMAiTKHEYEY.
    OrthoDBiEOG7HXD2Z.

    Family and domain databases

    Gene3Di1.10.418.10. 1 hit.
    InterProiIPR001715. CH-domain.
    IPR004953. EB1_C.
    IPR027328. MAPRE.
    [Graphical view]
    PANTHERiPTHR10623. PTHR10623. 1 hit.
    PfamiPF00307. CH. 1 hit.
    PF03271. EB1. 1 hit.
    [Graphical view]
    SUPFAMiSSF140612. SSF140612. 1 hit.
    SSF47576. SSF47576. 1 hit.
    PROSITEiPS51230. EB1_C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P40013-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAGIGESRT ELLTWLNGLL NLNYKKIEEC GTGAAYCQIM DSIYGDLPMN    50
    RVKFNATAEY EFQTNYKILQ SCFSRHGIEK TVYVDKLIRC KFQDNLEFLQ 100
    WLKKHWIRHK DESVYDPDAR RKYRPIITNN SATKPRTVSN PTTAKRSSST 150
    GTGSAMSGGL ATRHSSLGIN GSRKTSVTQG QLVAIQAELT KSQETIGSLN 200
    EEIEQYKGTV STLEIEREFY FNKLRDIEIL VHTTQDLINE GVYKFNDETI 250
    TGHGNGNGGA LLRFVKKVES ILYATAEGFE MNDGEDELND KNLGEHGTVP 300
    NQGGYANSNG EVNGNEGSNH DVIMQNDEGE VGVSNNLIID EETF 344
    Length:344
    Mass (Da):38,362
    Last modified:February 1, 1995 - v1
    Checksum:iC9087CB46A32BD74
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18778 Genomic DNA. Translation: AAB64549.1.
    BK006939 Genomic DNA. Translation: DAA07668.1.
    PIRiS50474.
    RefSeqiNP_010932.1. NM_001178907.1.

    Genome annotation databases

    EnsemblFungiiYER016W; YER016W; YER016W.
    GeneIDi856736.
    KEGGisce:YER016W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18778 Genomic DNA. Translation: AAB64549.1 .
    BK006939 Genomic DNA. Translation: DAA07668.1 .
    PIRi S50474.
    RefSeqi NP_010932.1. NM_001178907.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2QJX X-ray 1.90 A 1-124 [» ]
    4E61 X-ray 2.45 A/B 182-282 [» ]
    ProteinModelPortali P40013.
    SMRi P40013. Positions 8-124, 180-281.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36749. 461 interactions.
    DIPi DIP-1295N.
    IntActi P40013. 16 interactions.
    MINTi MINT-410426.
    STRINGi 4932.YER016W.

    Proteomic databases

    MaxQBi P40013.
    PaxDbi P40013.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER016W ; YER016W ; YER016W .
    GeneIDi 856736.
    KEGGi sce:YER016W.

    Organism-specific databases

    CYGDi YER016w.
    SGDi S000000818. BIM1.

    Phylogenomic databases

    eggNOGi COG5217.
    GeneTreei ENSGT00490000043329.
    HOGENOMi HOG000198048.
    KOi K10436.
    OMAi TKHEYEY.
    OrthoDBi EOG7HXD2Z.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30201-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P40013.
    NextBioi 982858.
    PROi P40013.

    Gene expression databases

    Genevestigatori P40013.

    Family and domain databases

    Gene3Di 1.10.418.10. 1 hit.
    InterProi IPR001715. CH-domain.
    IPR004953. EB1_C.
    IPR027328. MAPRE.
    [Graphical view ]
    PANTHERi PTHR10623. PTHR10623. 1 hit.
    Pfami PF00307. CH. 1 hit.
    PF03271. EB1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF140612. SSF140612. 1 hit.
    SSF47576. SSF47576. 1 hit.
    PROSITEi PS51230. EB1_C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "BIM1 encodes a microtubule-binding protein in yeast."
      Schwartz K., Richards K., Botstein D.
      Mol. Biol. Cell 8:2677-2691(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    6. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-157, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiBIM1_YEAST
    AccessioniPrimary (citable) accession number: P40013
    Secondary accession number(s): D3DLR4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3630 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3