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Protein

Protein BIM1

Gene

BIM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds microtubules.

GO - Molecular functioni

  1. microtubule plus-end binding Source: SGD
  2. protein homodimerization activity Source: SGD
  3. structural constituent of cytoskeleton Source: SGD

GO - Biological processi

  1. cell division Source: UniProtKB-KW
  2. microtubule depolymerization Source: SGD
  3. microtubule nucleation Source: SGD
  4. mitotic sister chromatid cohesion Source: SGD
  5. mitotic spindle assembly checkpoint Source: SGD
  6. negative regulation of microtubule depolymerization Source: SGD
  7. nuclear migration along microtubule Source: SGD
  8. positive regulation of microtubule polymerization Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-30201-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein BIM1
Gene namesi
Name:BIM1
Ordered Locus Names:YER016W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome V

Organism-specific databases

CYGDiYER016w.
SGDiS000000818. BIM1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasmic microtubule Source: SGD
  2. microtubule plus-end Source: SGD
  3. spindle Source: SGD
  4. spindle midzone Source: SGD
  5. spindle pole Source: SGD
  6. spindle pole body Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 344343Protein BIM1PRO_0000213432Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei157 – 1571Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP40013.
PaxDbiP40013.

Expressioni

Gene expression databases

GenevestigatoriP40013.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
DAM1P532672EBI-3614,EBI-23268

Protein-protein interaction databases

BioGridi36749. 461 interactions.
DIPiDIP-1295N.
IntActiP40013. 16 interactions.
MINTiMINT-410426.
STRINGi4932.YER016W.

Structurei

Secondary structure

1
344
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 2012Combined sources
Helixi27 – 326Combined sources
Helixi34 – 4411Combined sources
Helixi49 – 513Combined sources
Helixi59 – 7517Combined sources
Helixi84 – 874Combined sources
Turni88 – 903Combined sources
Helixi92 – 10918Combined sources
Helixi117 – 1204Combined sources
Turni121 – 1233Combined sources
Helixi182 – 23958Combined sources
Helixi260 – 27718Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2QJXX-ray1.90A1-124[»]
4E61X-ray2.45A/B182-282[»]
ProteinModelPortaliP40013.
SMRiP40013. Positions 8-124, 180-281.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40013.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 107100CHAdd
BLAST
Domaini188 – 28194EB1 C-terminalPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the MAPRE family.Curated
Contains 1 EB1 C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5217.
GeneTreeiENSGT00490000043329.
HOGENOMiHOG000198048.
InParanoidiP40013.
KOiK10436.
OMAiTKHEYEY.
OrthoDBiEOG7HXD2Z.

Family and domain databases

Gene3Di1.10.418.10. 1 hit.
InterProiIPR001715. CH-domain.
IPR004953. EB1_C.
IPR027328. MAPRE.
[Graphical view]
PANTHERiPTHR10623. PTHR10623. 1 hit.
PfamiPF00307. CH. 1 hit.
PF03271. EB1. 1 hit.
[Graphical view]
SUPFAMiSSF140612. SSF140612. 1 hit.
SSF47576. SSF47576. 1 hit.
PROSITEiPS51230. EB1_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40013-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAGIGESRT ELLTWLNGLL NLNYKKIEEC GTGAAYCQIM DSIYGDLPMN
60 70 80 90 100
RVKFNATAEY EFQTNYKILQ SCFSRHGIEK TVYVDKLIRC KFQDNLEFLQ
110 120 130 140 150
WLKKHWIRHK DESVYDPDAR RKYRPIITNN SATKPRTVSN PTTAKRSSST
160 170 180 190 200
GTGSAMSGGL ATRHSSLGIN GSRKTSVTQG QLVAIQAELT KSQETIGSLN
210 220 230 240 250
EEIEQYKGTV STLEIEREFY FNKLRDIEIL VHTTQDLINE GVYKFNDETI
260 270 280 290 300
TGHGNGNGGA LLRFVKKVES ILYATAEGFE MNDGEDELND KNLGEHGTVP
310 320 330 340
NQGGYANSNG EVNGNEGSNH DVIMQNDEGE VGVSNNLIID EETF
Length:344
Mass (Da):38,362
Last modified:February 1, 1995 - v1
Checksum:iC9087CB46A32BD74
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18778 Genomic DNA. Translation: AAB64549.1.
BK006939 Genomic DNA. Translation: DAA07668.1.
PIRiS50474.
RefSeqiNP_010932.1. NM_001178907.1.

Genome annotation databases

EnsemblFungiiYER016W; YER016W; YER016W.
GeneIDi856736.
KEGGisce:YER016W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18778 Genomic DNA. Translation: AAB64549.1.
BK006939 Genomic DNA. Translation: DAA07668.1.
PIRiS50474.
RefSeqiNP_010932.1. NM_001178907.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2QJXX-ray1.90A1-124[»]
4E61X-ray2.45A/B182-282[»]
ProteinModelPortaliP40013.
SMRiP40013. Positions 8-124, 180-281.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36749. 461 interactions.
DIPiDIP-1295N.
IntActiP40013. 16 interactions.
MINTiMINT-410426.
STRINGi4932.YER016W.

Proteomic databases

MaxQBiP40013.
PaxDbiP40013.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER016W; YER016W; YER016W.
GeneIDi856736.
KEGGisce:YER016W.

Organism-specific databases

CYGDiYER016w.
SGDiS000000818. BIM1.

Phylogenomic databases

eggNOGiCOG5217.
GeneTreeiENSGT00490000043329.
HOGENOMiHOG000198048.
InParanoidiP40013.
KOiK10436.
OMAiTKHEYEY.
OrthoDBiEOG7HXD2Z.

Enzyme and pathway databases

BioCyciYEAST:G3O-30201-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP40013.
NextBioi982858.
PROiP40013.

Gene expression databases

GenevestigatoriP40013.

Family and domain databases

Gene3Di1.10.418.10. 1 hit.
InterProiIPR001715. CH-domain.
IPR004953. EB1_C.
IPR027328. MAPRE.
[Graphical view]
PANTHERiPTHR10623. PTHR10623. 1 hit.
PfamiPF00307. CH. 1 hit.
PF03271. EB1. 1 hit.
[Graphical view]
SUPFAMiSSF140612. SSF140612. 1 hit.
SSF47576. SSF47576. 1 hit.
PROSITEiPS51230. EB1_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "BIM1 encodes a microtubule-binding protein in yeast."
    Schwartz K., Richards K., Botstein D.
    Mol. Biol. Cell 8:2677-2691(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  6. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-157, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBIM1_YEAST
AccessioniPrimary (citable) accession number: P40013
Secondary accession number(s): D3DLR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: February 4, 2015
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3630 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.