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P40013

- BIM1_YEAST

UniProt

P40013 - BIM1_YEAST

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Protein
Protein BIM1
Gene
BIM1, YER016W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Binds microtubules.

GO - Molecular functioni

  1. microtubule plus-end binding Source: SGD
  2. protein binding Source: IntAct
  3. protein homodimerization activity Source: SGD
  4. structural constituent of cytoskeleton Source: SGD

GO - Biological processi

  1. microtubule depolymerization Source: SGD
  2. microtubule nucleation Source: SGD
  3. mitotic sister chromatid cohesion Source: SGD
  4. mitotic spindle assembly checkpoint Source: SGD
  5. negative regulation of microtubule depolymerization Source: SGD
  6. nuclear migration along microtubule Source: SGD
  7. positive regulation of microtubule polymerization Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-30201-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein BIM1
Gene namesi
Name:BIM1
Ordered Locus Names:YER016W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER016w.
SGDiS000000818. BIM1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasmic microtubule Source: SGD
  2. microtubule plus-end Source: SGD
  3. spindle Source: SGD
  4. spindle midzone Source: SGD
  5. spindle pole Source: SGD
  6. spindle pole body Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 344343Protein BIM1
PRO_0000213432Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei157 – 1571Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP40013.
PaxDbiP40013.

Expressioni

Gene expression databases

GenevestigatoriP40013.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
DAM1P532672EBI-3614,EBI-23268

Protein-protein interaction databases

BioGridi36749. 461 interactions.
DIPiDIP-1295N.
IntActiP40013. 16 interactions.
MINTiMINT-410426.
STRINGi4932.YER016W.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 2012
Helixi27 – 326
Helixi34 – 4411
Helixi49 – 513
Helixi59 – 7517
Helixi84 – 874
Turni88 – 903
Helixi92 – 10918
Helixi117 – 1204
Turni121 – 1233
Helixi182 – 23958
Helixi260 – 27718

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2QJXX-ray1.90A1-124[»]
4E61X-ray2.45A/B182-282[»]
ProteinModelPortaliP40013.
SMRiP40013. Positions 8-124, 180-281.

Miscellaneous databases

EvolutionaryTraceiP40013.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 107100CH
Add
BLAST
Domaini188 – 28194EB1 C-terminal
Add
BLAST

Sequence similaritiesi

Belongs to the MAPRE family.

Phylogenomic databases

eggNOGiCOG5217.
GeneTreeiENSGT00490000043329.
HOGENOMiHOG000198048.
KOiK10436.
OMAiTKHEYEY.
OrthoDBiEOG7HXD2Z.

Family and domain databases

Gene3Di1.10.418.10. 1 hit.
InterProiIPR001715. CH-domain.
IPR004953. EB1_C.
IPR027328. MAPRE.
[Graphical view]
PANTHERiPTHR10623. PTHR10623. 1 hit.
PfamiPF00307. CH. 1 hit.
PF03271. EB1. 1 hit.
[Graphical view]
SUPFAMiSSF140612. SSF140612. 1 hit.
SSF47576. SSF47576. 1 hit.
PROSITEiPS51230. EB1_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40013-1 [UniParc]FASTAAdd to Basket

« Hide

MSAGIGESRT ELLTWLNGLL NLNYKKIEEC GTGAAYCQIM DSIYGDLPMN    50
RVKFNATAEY EFQTNYKILQ SCFSRHGIEK TVYVDKLIRC KFQDNLEFLQ 100
WLKKHWIRHK DESVYDPDAR RKYRPIITNN SATKPRTVSN PTTAKRSSST 150
GTGSAMSGGL ATRHSSLGIN GSRKTSVTQG QLVAIQAELT KSQETIGSLN 200
EEIEQYKGTV STLEIEREFY FNKLRDIEIL VHTTQDLINE GVYKFNDETI 250
TGHGNGNGGA LLRFVKKVES ILYATAEGFE MNDGEDELND KNLGEHGTVP 300
NQGGYANSNG EVNGNEGSNH DVIMQNDEGE VGVSNNLIID EETF 344
Length:344
Mass (Da):38,362
Last modified:February 1, 1995 - v1
Checksum:iC9087CB46A32BD74
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18778 Genomic DNA. Translation: AAB64549.1.
BK006939 Genomic DNA. Translation: DAA07668.1.
PIRiS50474.
RefSeqiNP_010932.1. NM_001178907.1.

Genome annotation databases

EnsemblFungiiYER016W; YER016W; YER016W.
GeneIDi856736.
KEGGisce:YER016W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18778 Genomic DNA. Translation: AAB64549.1 .
BK006939 Genomic DNA. Translation: DAA07668.1 .
PIRi S50474.
RefSeqi NP_010932.1. NM_001178907.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2QJX X-ray 1.90 A 1-124 [» ]
4E61 X-ray 2.45 A/B 182-282 [» ]
ProteinModelPortali P40013.
SMRi P40013. Positions 8-124, 180-281.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36749. 461 interactions.
DIPi DIP-1295N.
IntActi P40013. 16 interactions.
MINTi MINT-410426.
STRINGi 4932.YER016W.

Proteomic databases

MaxQBi P40013.
PaxDbi P40013.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER016W ; YER016W ; YER016W .
GeneIDi 856736.
KEGGi sce:YER016W.

Organism-specific databases

CYGDi YER016w.
SGDi S000000818. BIM1.

Phylogenomic databases

eggNOGi COG5217.
GeneTreei ENSGT00490000043329.
HOGENOMi HOG000198048.
KOi K10436.
OMAi TKHEYEY.
OrthoDBi EOG7HXD2Z.

Enzyme and pathway databases

BioCyci YEAST:G3O-30201-MONOMER.

Miscellaneous databases

EvolutionaryTracei P40013.
NextBioi 982858.
PROi P40013.

Gene expression databases

Genevestigatori P40013.

Family and domain databases

Gene3Di 1.10.418.10. 1 hit.
InterProi IPR001715. CH-domain.
IPR004953. EB1_C.
IPR027328. MAPRE.
[Graphical view ]
PANTHERi PTHR10623. PTHR10623. 1 hit.
Pfami PF00307. CH. 1 hit.
PF03271. EB1. 1 hit.
[Graphical view ]
SUPFAMi SSF140612. SSF140612. 1 hit.
SSF47576. SSF47576. 1 hit.
PROSITEi PS51230. EB1_C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "BIM1 encodes a microtubule-binding protein in yeast."
    Schwartz K., Richards K., Botstein D.
    Mol. Biol. Cell 8:2677-2691(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  6. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-157, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBIM1_YEAST
AccessioniPrimary (citable) accession number: P40013
Secondary accession number(s): D3DLR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3630 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

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