Reviewed,
UniProtKB/Swiss-Prot P40012 (PPOX_YEAST)
Last modified
June 16, 2009.
Version 69.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Protoporphyrinogen oxidase Short name=PPO EC=1.3.3.4 | ||||
| Gene names |
| ||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||
| Taxonomic identifier | 4932 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 539 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX. |
| Catalytic activity | Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2. |
| Cofactor | FAD. Binds 1 mole of FAD per mole of enzyme. |
| Pathway | |
| Subcellular location | Mitochondrion inner membrane. Note: Bound to the mitochondrial inner membrane with its active site facing the cytosolic side. Ref.3 |
| Miscellaneous | Present with 5350 molecules/cell in log phase SD medium. Ref.4 |
| Sequence similarities | Belongs to the protoporphyrinogen oxidase family. |
| Biophysicochemical properties | pH dependence: Optimum pH is 7.2. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Heme biosynthesis Porphyrin biosynthesis |
| Cellular component | Membrane Mitochondrion Mitochondrion inner membrane |
| Ligand | FAD Flavoprotein |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | heme biosynthetic process Inferred from mutant phenotype. Source: SGD oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW thiamin biosynthetic processInferred from electronic annotation. Source: InterPro |
| Cellular component | mitochondrial inner membrane Ref.3 Inferred from direct assay. Source: SGD |
| Molecular function | protoporphyrinogen oxidase activity Ref.3 Inferred from direct assay. Source: SGD |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 539 | 539 | Protoporphyrinogen oxidase | PRO_0000135274 | |||||
Regions | |||||||||
| Nucleotide binding | 18 – 23 | 6 | FAD Potential | ||||||
Experimental info | |||||||||
| Mutagenesis | 422 | 1 | L → P in HEM14-1; loss of activity. | ||||||
| Mutagenesis | 424 | 1 | K → E in HEM14-1; loss of activity. | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning and characterization of the yeast HEM14 gene coding for protoporphyrinogen oxidase, the molecular target of diphenyl ether-type herbicides." Camadro J.-M., Labbe P. J. Biol. Chem. 271:9120-9128(1996) [PubMed: 8621563] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W.Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [3] | "Purification and properties of protoporphyrinogen oxidase from the yeast Saccharomyces cerevisiae. Mitochondrial location and evidence for a precursor form of the protein." Camadro J.-M., Thome F., Brouillet N., Labbe P. J. Biol. Chem. 269:32085-32091(1994) [PubMed: 7798202] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: ATCC 25657 / D273-10B. |
| [4] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| Z71381 Genomic DNA. Translation: CAA95981.1. U18778 Genomic DNA. Translation: AAB64547.1. | |
| PIR | S50472. |
| RefSeq | NP_010930.1. |
3D structure databases | |
| ModBase | Search... |
Proteomic databases | |
| PeptideAtlas | P40012. |
Genome annotation databases | |
| Ensembl | YER014W. Saccharomyces cerevisiae. [Contig view] |
| GeneID | 856733. |
| GenomeReviews | Gene locus YER014W in contig U00092_GR. |
| KEGG | sce:YER014W. |
| NMPDR | fig|4932.3.peg.1989. |
Organism-specific databases | |
| CYGD | YER014w. |
| SGD | S000000816. HEM14. |
| Yeast-GFP | Search... |
Phylogenomic databases | |
| HOGENOM | P40012. |
| OMA | P40012. VQVPNSI. |
Enzyme and pathway databases | |
| BRENDA | 1.3.3.4. 250. |
Gene expression databases | |
| GermOnline | YER014W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR004572. Protoporphyrinogen_oxidase. IPR002922. Thi4. [Graphical view] |
| Pfam | PF01946. Thi4. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00562. proto_IX_ox. 1 hit. |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 982849. |
Entry information
| Entry name | PPOX_YEAST | ||||||||
| Accession | Primary (citable) accession number: P40012 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome V Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names |

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