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P40012

- PPOX_YEAST

UniProt

P40012 - PPOX_YEAST

Protein

Protoporphyrinogen oxidase

Gene

HEM14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.

    Catalytic activityi

    Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.

    Cofactori

    Binds 1 FAD per subunit.

    pH dependencei

    Optimum pH is 7.2.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei51 – 511FAD; via amide nitrogenBy similarity
    Binding sitei300 – 3001FAD; via amide nitrogen and carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi18 – 236FADBy similarity
    Nucleotide bindingi43 – 442FADBy similarity
    Nucleotide bindingi70 – 734FADBy similarity
    Nucleotide bindingi521 – 5233FADBy similarity

    GO - Molecular functioni

    1. oxygen-dependent protoporphyrinogen oxidase activity Source: SGD

    GO - Biological processi

    1. heme biosynthetic process Source: SGD
    2. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Heme biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciYEAST:YER014W-MONOMER.
    SABIO-RKP40012.
    UniPathwayiUPA00251; UER00324.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protoporphyrinogen oxidase (EC:1.3.3.4)
    Short name:
    PPO
    Gene namesi
    Name:HEM14
    Ordered Locus Names:YER014W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYER014w.
    SGDiS000000816. HEM14.

    Subcellular locationi

    Mitochondrion inner membrane 1 Publication
    Note: Bound to the mitochondrial inner membrane with its active site facing the cytosolic side.

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: SGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi422 – 4221L → P in HEM14-1; loss of activity.
    Mutagenesisi424 – 4241K → E in HEM14-1; loss of activity.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 539539Protoporphyrinogen oxidasePRO_0000135274Add
    BLAST

    Proteomic databases

    MaxQBiP40012.
    PaxDbiP40012.
    PeptideAtlasiP40012.

    Expressioni

    Gene expression databases

    GenevestigatoriP40012.

    Interactioni

    Protein-protein interaction databases

    BioGridi36746. 32 interactions.
    MINTiMINT-4483733.
    STRINGi4932.YER014W.

    Structurei

    3D structure databases

    ProteinModelPortaliP40012.
    SMRiP40012. Positions 13-537.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the protoporphyrinogen oxidase family.Curated

    Phylogenomic databases

    eggNOGiCOG1232.
    GeneTreeiENSGT00390000008744.
    HOGENOMiHOG000093387.
    KOiK00231.
    OMAiNKITIMM.
    OrthoDBiEOG7JQBX8.

    Family and domain databases

    Gene3Di3.90.660.20. 1 hit.
    InterProiIPR027418. PPOX_C.
    IPR004572. Protoporphyrinogen_oxidase.
    [Graphical view]
    TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P40012-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLLPLTKLKP RAKVAVVGGG VSGLCFTYFL SKLRPDVEIT LFESQNRTGG    50
    WIYSCNTRDM SGNPIMLEKG PRTLRGVSDG TVLIMDTLKD LGKEAVIQSI 100
    DKGCIADKKF LLDPSDKLVQ VPNSISTTVK FLLNPLGKGL ITGMMGEWFR 150
    KKSPHPGQDE SVESICDRRF GNNYISNNMI SALLRGIYGD DVSLLSAKRT 200
    FKKIYYNELK HGSNTQAMID NMRGKSRSKK TENLHQSLTG CLNDYSNAFG 250
    KDRSKLLDLS NTLKKYPMLG LAGGLETFPK IVRNALNEFK NVKIVTGNPV 300
    TQIMKRPANE TTIGLKAKSG DQYETFDHLR LTITPPKIAK LLPKDQNSLS 350
    KLLDEIQSNT IILVNYYLPN KDVIDADLQG FGYLVPKSNK NPGKLLGVIF 400
    DSVIERNFKP LFDKLSTNPN ALNKYTKVTA MIGGCMLNEH GVPVVPSREV 450
    TINAVKDALN NHLGISNKDL EAGQWEFTIA DRCLPRFHVG YDAWQERAER 500
    KLQESYGQTV SVGGMGFSRS PGVPDVIVDG FNDALQLSK 539
    Length:539
    Mass (Da):59,703
    Last modified:February 1, 1995 - v1
    Checksum:i5ECCBD1C033BA5B1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71381 Genomic DNA. Translation: CAA95981.1.
    U18778 Genomic DNA. Translation: AAB64547.1.
    BK006939 Genomic DNA. Translation: DAA07665.1.
    PIRiS50472.
    RefSeqiNP_010930.1. NM_001178905.1.

    Genome annotation databases

    EnsemblFungiiYER014W; YER014W; YER014W.
    GeneIDi856733.
    KEGGisce:YER014W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71381 Genomic DNA. Translation: CAA95981.1 .
    U18778 Genomic DNA. Translation: AAB64547.1 .
    BK006939 Genomic DNA. Translation: DAA07665.1 .
    PIRi S50472.
    RefSeqi NP_010930.1. NM_001178905.1.

    3D structure databases

    ProteinModelPortali P40012.
    SMRi P40012. Positions 13-537.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36746. 32 interactions.
    MINTi MINT-4483733.
    STRINGi 4932.YER014W.

    Proteomic databases

    MaxQBi P40012.
    PaxDbi P40012.
    PeptideAtlasi P40012.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER014W ; YER014W ; YER014W .
    GeneIDi 856733.
    KEGGi sce:YER014W.

    Organism-specific databases

    CYGDi YER014w.
    SGDi S000000816. HEM14.

    Phylogenomic databases

    eggNOGi COG1232.
    GeneTreei ENSGT00390000008744.
    HOGENOMi HOG000093387.
    KOi K00231.
    OMAi NKITIMM.
    OrthoDBi EOG7JQBX8.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00324 .
    BioCyci YEAST:YER014W-MONOMER.
    SABIO-RK P40012.

    Miscellaneous databases

    NextBioi 982849.
    PROi P40012.

    Gene expression databases

    Genevestigatori P40012.

    Family and domain databases

    Gene3Di 3.90.660.20. 1 hit.
    InterProi IPR027418. PPOX_C.
    IPR004572. Protoporphyrinogen_oxidase.
    [Graphical view ]
    TIGRFAMsi TIGR00562. proto_IX_ox. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the yeast HEM14 gene coding for protoporphyrinogen oxidase, the molecular target of diphenyl ether-type herbicides."
      Camadro J.-M., Labbe P.
      J. Biol. Chem. 271:9120-9128(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Purification and properties of protoporphyrinogen oxidase from the yeast Saccharomyces cerevisiae. Mitochondrial location and evidence for a precursor form of the protein."
      Camadro J.-M., Thome F., Brouillet N., Labbe P.
      J. Biol. Chem. 269:32085-32091(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
      Strain: ATCC 25657 / D273-10B.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPPOX_YEAST
    AccessioniPrimary (citable) accession number: P40012
    Secondary accession number(s): D3DLR1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 5350 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3