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Reviewed, UniProtKB/Swiss-Prot P40012 (PPOX_YEAST)

Last modified June 16, 2009. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Protoporphyrinogen oxidase
      Short name=PPO
    EC=1.3.3.4
Gene names
Name: HEM14
Ordered Locus Names: YER014W
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length539 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.

Catalytic activity

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.

Cofactor

FAD. Binds 1 mole of FAD per mole of enzyme.

Pathway

Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.

Subcellular location

Mitochondrion inner membrane. Note: Bound to the mitochondrial inner membrane with its active site facing the cytosolic side. Ref.3

Miscellaneous

Present with 5350 molecules/cell in log phase SD medium. Ref.4

Sequence similarities

Belongs to the protoporphyrinogen oxidase family.

Biophysicochemical properties

pH dependence:

Optimum pH is 7.2.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 539539Protoporphyrinogen oxidase
PRO_0000135274

Regions

Nucleotide binding18 – 236FAD Potential

Experimental info

Mutagenesis4221L → P in HEM14-1; loss of activity.
Mutagenesis4241K → E in HEM14-1; loss of activity.

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Sequences

Sequence LengthMass (Da)Tools
P40012-1 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 5ECCBD1C033BA5B1

FASTA53959,703
        10         20         30         40         50         60 
MLLPLTKLKP RAKVAVVGGG VSGLCFTYFL SKLRPDVEIT LFESQNRTGG WIYSCNTRDM 

        70         80         90        100        110        120 
SGNPIMLEKG PRTLRGVSDG TVLIMDTLKD LGKEAVIQSI DKGCIADKKF LLDPSDKLVQ 

       130        140        150        160        170        180 
VPNSISTTVK FLLNPLGKGL ITGMMGEWFR KKSPHPGQDE SVESICDRRF GNNYISNNMI 

       190        200        210        220        230        240 
SALLRGIYGD DVSLLSAKRT FKKIYYNELK HGSNTQAMID NMRGKSRSKK TENLHQSLTG 

       250        260        270        280        290        300 
CLNDYSNAFG KDRSKLLDLS NTLKKYPMLG LAGGLETFPK IVRNALNEFK NVKIVTGNPV 

       310        320        330        340        350        360 
TQIMKRPANE TTIGLKAKSG DQYETFDHLR LTITPPKIAK LLPKDQNSLS KLLDEIQSNT 

       370        380        390        400        410        420 
IILVNYYLPN KDVIDADLQG FGYLVPKSNK NPGKLLGVIF DSVIERNFKP LFDKLSTNPN 

       430        440        450        460        470        480 
ALNKYTKVTA MIGGCMLNEH GVPVVPSREV TINAVKDALN NHLGISNKDL EAGQWEFTIA 

       490        500        510        520        530 
DRCLPRFHVG YDAWQERAER KLQESYGQTV SVGGMGFSRS PGVPDVIVDG FNDALQLSK

« Hide

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References

« Hide 'large scale' references
[1]"Cloning and characterization of the yeast HEM14 gene coding for protoporphyrinogen oxidase, the molecular target of diphenyl ether-type herbicides."
Camadro J.-M., Labbe P.
J. Biol. Chem. 271:9120-9128(1996) [PubMed: 8621563] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed: 9169868] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]"Purification and properties of protoporphyrinogen oxidase from the yeast Saccharomyces cerevisiae. Mitochondrial location and evidence for a precursor form of the protein."
Camadro J.-M., Thome F., Brouillet N., Labbe P.
J. Biol. Chem. 269:32085-32091(1994) [PubMed: 7798202] [Abstract]
Cited for: SUBCELLULAR LOCATION.
Strain: ATCC 25657 / D273-10B.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

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Cross-references

Sequence databases

Z71381 Genomic DNA. Translation: CAA95981.1.
U18778 Genomic DNA. Translation: AAB64547.1.
PIRS50472.
RefSeqNP_010930.1.

3D structure databases

ModBaseSearch...

Proteomic databases

PeptideAtlasP40012.

Genome annotation databases

EnsemblYER014W. Saccharomyces cerevisiae. [Contig view]
GeneID856733.
GenomeReviewsGene locus YER014W in contig U00092_GR.
KEGGsce:YER014W.
NMPDRfig|4932.3.peg.1989.

Organism-specific databases

CYGDYER014w.
SGDS000000816. HEM14.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMP40012.
OMAP40012. VQVPNSI.

Enzyme and pathway databases

BRENDA1.3.3.4. 250.

Gene expression databases

GermOnlineYER014W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR004572. Protoporphyrinogen_oxidase.
IPR002922. Thi4.
[Graphical view]
PfamPF01946. Thi4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00562. proto_IX_ox. 1 hit.
ProtoNetSearch...

Other Resources

NextBio982849.

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Entry information

Entry namePPOX_YEAST
AccessionPrimary (citable) accession number: P40012
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 16, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents