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Protein

Protoporphyrinogen oxidase

Gene

HEM14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.

Catalytic activityi

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.

Cofactori

FADNote: Binds 1 FAD per subunit.

pH dependencei

Optimum pH is 7.2.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei51 – 511FAD; via amide nitrogenBy similarity
Binding sitei300 – 3001FAD; via amide nitrogen and carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 236FADBy similarity
Nucleotide bindingi43 – 442FADBy similarity
Nucleotide bindingi70 – 734FADBy similarity
Nucleotide bindingi521 – 5233FADBy similarity

GO - Molecular functioni

  1. oxygen-dependent protoporphyrinogen oxidase activity Source: SGD

GO - Biological processi

  1. heme biosynthetic process Source: SGD
  2. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciYEAST:YER014W-MONOMER.
ReactomeiREACT_347076. Heme biosynthesis.
SABIO-RKP40012.
UniPathwayiUPA00251; UER00324.

Names & Taxonomyi

Protein namesi
Recommended name:
Protoporphyrinogen oxidase (EC:1.3.3.4)
Short name:
PPO
Gene namesi
Name:HEM14
Ordered Locus Names:YER014W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome V

Organism-specific databases

CYGDiYER014w.
EuPathDBiFungiDB:YER014W.
SGDiS000000816. HEM14.

Subcellular locationi

  1. Mitochondrion inner membrane 1 Publication

  2. Note: Bound to the mitochondrial inner membrane with its active site facing the cytosolic side.

GO - Cellular componenti

  1. mitochondrial inner membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi422 – 4221L → P in HEM14-1; loss of activity.
Mutagenesisi424 – 4241K → E in HEM14-1; loss of activity.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 539539Protoporphyrinogen oxidasePRO_0000135274Add
BLAST

Proteomic databases

MaxQBiP40012.
PaxDbiP40012.
PeptideAtlasiP40012.

Expressioni

Gene expression databases

GenevestigatoriP40012.

Interactioni

Protein-protein interaction databases

BioGridi36746. 32 interactions.
MINTiMINT-4483733.
STRINGi4932.YER014W.

Structurei

3D structure databases

ProteinModelPortaliP40012.
SMRiP40012. Positions 14-537.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the protoporphyrinogen oxidase family.Curated

Phylogenomic databases

eggNOGiCOG1232.
GeneTreeiENSGT00390000008744.
HOGENOMiHOG000093387.
InParanoidiP40012.
KOiK00231.
OMAiLMGGHYY.
OrthoDBiEOG7JQBX8.

Family and domain databases

Gene3Di3.90.660.20. 1 hit.
InterProiIPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.

Sequencei

Sequence statusi: Complete.

P40012-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLPLTKLKP RAKVAVVGGG VSGLCFTYFL SKLRPDVEIT LFESQNRTGG
60 70 80 90 100
WIYSCNTRDM SGNPIMLEKG PRTLRGVSDG TVLIMDTLKD LGKEAVIQSI
110 120 130 140 150
DKGCIADKKF LLDPSDKLVQ VPNSISTTVK FLLNPLGKGL ITGMMGEWFR
160 170 180 190 200
KKSPHPGQDE SVESICDRRF GNNYISNNMI SALLRGIYGD DVSLLSAKRT
210 220 230 240 250
FKKIYYNELK HGSNTQAMID NMRGKSRSKK TENLHQSLTG CLNDYSNAFG
260 270 280 290 300
KDRSKLLDLS NTLKKYPMLG LAGGLETFPK IVRNALNEFK NVKIVTGNPV
310 320 330 340 350
TQIMKRPANE TTIGLKAKSG DQYETFDHLR LTITPPKIAK LLPKDQNSLS
360 370 380 390 400
KLLDEIQSNT IILVNYYLPN KDVIDADLQG FGYLVPKSNK NPGKLLGVIF
410 420 430 440 450
DSVIERNFKP LFDKLSTNPN ALNKYTKVTA MIGGCMLNEH GVPVVPSREV
460 470 480 490 500
TINAVKDALN NHLGISNKDL EAGQWEFTIA DRCLPRFHVG YDAWQERAER
510 520 530
KLQESYGQTV SVGGMGFSRS PGVPDVIVDG FNDALQLSK
Length:539
Mass (Da):59,703
Last modified:February 1, 1995 - v1
Checksum:i5ECCBD1C033BA5B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71381 Genomic DNA. Translation: CAA95981.1.
U18778 Genomic DNA. Translation: AAB64547.1.
BK006939 Genomic DNA. Translation: DAA07665.1.
PIRiS50472.
RefSeqiNP_010930.1. NM_001178905.1.

Genome annotation databases

EnsemblFungiiYER014W; YER014W; YER014W.
GeneIDi856733.
KEGGisce:YER014W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71381 Genomic DNA. Translation: CAA95981.1.
U18778 Genomic DNA. Translation: AAB64547.1.
BK006939 Genomic DNA. Translation: DAA07665.1.
PIRiS50472.
RefSeqiNP_010930.1. NM_001178905.1.

3D structure databases

ProteinModelPortaliP40012.
SMRiP40012. Positions 14-537.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36746. 32 interactions.
MINTiMINT-4483733.
STRINGi4932.YER014W.

Proteomic databases

MaxQBiP40012.
PaxDbiP40012.
PeptideAtlasiP40012.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER014W; YER014W; YER014W.
GeneIDi856733.
KEGGisce:YER014W.

Organism-specific databases

CYGDiYER014w.
EuPathDBiFungiDB:YER014W.
SGDiS000000816. HEM14.

Phylogenomic databases

eggNOGiCOG1232.
GeneTreeiENSGT00390000008744.
HOGENOMiHOG000093387.
InParanoidiP40012.
KOiK00231.
OMAiLMGGHYY.
OrthoDBiEOG7JQBX8.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00324.
BioCyciYEAST:YER014W-MONOMER.
ReactomeiREACT_347076. Heme biosynthesis.
SABIO-RKP40012.

Miscellaneous databases

NextBioi982849.
PROiP40012.

Gene expression databases

GenevestigatoriP40012.

Family and domain databases

Gene3Di3.90.660.20. 1 hit.
InterProiIPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the yeast HEM14 gene coding for protoporphyrinogen oxidase, the molecular target of diphenyl ether-type herbicides."
    Camadro J.-M., Labbe P.
    J. Biol. Chem. 271:9120-9128(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Purification and properties of protoporphyrinogen oxidase from the yeast Saccharomyces cerevisiae. Mitochondrial location and evidence for a precursor form of the protein."
    Camadro J.-M., Thome F., Brouillet N., Labbe P.
    J. Biol. Chem. 269:32085-32091(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: ATCC 25657 / D273-10B.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPPOX_YEAST
AccessioniPrimary (citable) accession number: P40012
Secondary accession number(s): D3DLR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 29, 2015
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5350 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.