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Protein

Protoporphyrinogen oxidase

Gene

HEM14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.

Miscellaneous

Present with 5350 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.

Cofactori

FADNote: Binds 1 FAD per subunit.

pH dependencei

Optimum pH is 7.2.

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX.
Proteins known to be involved in this subpathway in this organism are:
  1. Protoporphyrinogen oxidase (HEM14)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei51FAD; via amide nitrogenBy similarity1
Binding sitei300FAD; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 23FADBy similarity6
Nucleotide bindingi43 – 44FADBy similarity2
Nucleotide bindingi70 – 73FADBy similarity4
Nucleotide bindingi521 – 523FADBy similarity3

GO - Molecular functioni

  • oxygen-dependent protoporphyrinogen oxidase activity Source: SGD

GO - Biological processi

  • heme biosynthetic process Source: SGD
  • protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionOxidoreductase
Biological processHeme biosynthesis, Porphyrin biosynthesis
LigandFAD, Flavoprotein

Enzyme and pathway databases

BioCyciYEAST:YER014W-MONOMER
ReactomeiR-SCE-189451 Heme biosynthesis
SABIO-RKiP40012
UniPathwayiUPA00251; UER00324

Names & Taxonomyi

Protein namesi
Recommended name:
Protoporphyrinogen oxidase (EC:1.3.3.4)
Short name:
PPO
Gene namesi
Name:HEM14
Ordered Locus Names:YER014W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER014W
SGDiS000000816 HEM14

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi422L → P in HEM14-1; loss of activity. 1
Mutagenesisi424K → E in HEM14-1; loss of activity. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001352741 – 539Protoporphyrinogen oxidaseAdd BLAST539

Proteomic databases

MaxQBiP40012
PaxDbiP40012
PRIDEiP40012

PTM databases

SwissPalmiP40012

Interactioni

Protein-protein interaction databases

BioGridi36746, 33 interactors
MINTiP40012
STRINGi4932.YER014W

Structurei

3D structure databases

ProteinModelPortaliP40012
SMRiP40012
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the protoporphyrinogen oxidase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000008744
HOGENOMiHOG000093387
InParanoidiP40012
KOiK00231
OMAiPMICGIC
OrthoDBiEOG092C22OH

Family and domain databases

Gene3Di3.50.50.60, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR004572 Protoporphyrinogen_oxidase
SUPFAMiSSF51905 SSF51905, 3 hits
TIGRFAMsiTIGR00562 proto_IX_ox, 1 hit

Sequencei

Sequence statusi: Complete.

P40012-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLPLTKLKP RAKVAVVGGG VSGLCFTYFL SKLRPDVEIT LFESQNRTGG
60 70 80 90 100
WIYSCNTRDM SGNPIMLEKG PRTLRGVSDG TVLIMDTLKD LGKEAVIQSI
110 120 130 140 150
DKGCIADKKF LLDPSDKLVQ VPNSISTTVK FLLNPLGKGL ITGMMGEWFR
160 170 180 190 200
KKSPHPGQDE SVESICDRRF GNNYISNNMI SALLRGIYGD DVSLLSAKRT
210 220 230 240 250
FKKIYYNELK HGSNTQAMID NMRGKSRSKK TENLHQSLTG CLNDYSNAFG
260 270 280 290 300
KDRSKLLDLS NTLKKYPMLG LAGGLETFPK IVRNALNEFK NVKIVTGNPV
310 320 330 340 350
TQIMKRPANE TTIGLKAKSG DQYETFDHLR LTITPPKIAK LLPKDQNSLS
360 370 380 390 400
KLLDEIQSNT IILVNYYLPN KDVIDADLQG FGYLVPKSNK NPGKLLGVIF
410 420 430 440 450
DSVIERNFKP LFDKLSTNPN ALNKYTKVTA MIGGCMLNEH GVPVVPSREV
460 470 480 490 500
TINAVKDALN NHLGISNKDL EAGQWEFTIA DRCLPRFHVG YDAWQERAER
510 520 530
KLQESYGQTV SVGGMGFSRS PGVPDVIVDG FNDALQLSK
Length:539
Mass (Da):59,703
Last modified:February 1, 1995 - v1
Checksum:i5ECCBD1C033BA5B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71381 Genomic DNA Translation: CAA95981.1
U18778 Genomic DNA Translation: AAB64547.1
BK006939 Genomic DNA Translation: DAA07665.1
PIRiS50472
RefSeqiNP_010930.1, NM_001178905.1

Genome annotation databases

EnsemblFungiiYER014W; YER014W; YER014W
GeneIDi856733
KEGGisce:YER014W

Similar proteinsi

Entry informationi

Entry nameiPPOX_YEAST
AccessioniPrimary (citable) accession number: P40012
Secondary accession number(s): D3DLR1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names
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Main funding by: National Institutes of Health