P40012 (PPOX_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protoporphyrinogen oxidase
Short name=PPO
EC=1.3.3.4

Gene names

Name:	HEM14
Ordered Locus Names:	YER014W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	539 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.
Catalytic activity	Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.
Cofactor	FAD. Binds 1 mole of FAD per mole of enzyme.
Pathway	Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.
Subcellular location	Mitochondrion inner membrane. Note: Bound to the mitochondrial inner membrane with its active site facing the cytosolic side. Ref.4
Miscellaneous	Present with 5350 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the protoporphyrinogen oxidase family.
Biophysicochemical properties	pH dependence: Optimum pH is 7.2.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	heme biosynthetic process Inferred from mutant phenotype PubMed 7035824. Source: SGD protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	mitochondrial inner membrane Inferred from direct assay Ref.4. Source: SGD
Molecular_function	oxygen-dependent protoporphyrinogen oxidase activity Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 539	539	Protoporphyrinogen oxidase		PRO_0000135274
Regions
	Nucleotide binding	18 – 23	6	FAD Potential
Experimental info
	Mutagenesis	422	1	L → P in HEM14-1; loss of activity.
	Mutagenesis	424	1	K → E in HEM14-1; loss of activity.

Sequences

	Sequence		Length	Mass (Da)
	P40012 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 5ECCBD1C033BA5B1	FASTA	539	59,703
	10 20 30 40 50 60 MLLPLTKLKP RAKVAVVGGG VSGLCFTYFL SKLRPDVEIT LFESQNRTGG WIYSCNTRDM 70 80 90 100 110 120 SGNPIMLEKG PRTLRGVSDG TVLIMDTLKD LGKEAVIQSI DKGCIADKKF LLDPSDKLVQ 130 140 150 160 170 180 VPNSISTTVK FLLNPLGKGL ITGMMGEWFR KKSPHPGQDE SVESICDRRF GNNYISNNMI 190 200 210 220 230 240 SALLRGIYGD DVSLLSAKRT FKKIYYNELK HGSNTQAMID NMRGKSRSKK TENLHQSLTG 250 260 270 280 290 300 CLNDYSNAFG KDRSKLLDLS NTLKKYPMLG LAGGLETFPK IVRNALNEFK NVKIVTGNPV 310 320 330 340 350 360 TQIMKRPANE TTIGLKAKSG DQYETFDHLR LTITPPKIAK LLPKDQNSLS KLLDEIQSNT 370 380 390 400 410 420 IILVNYYLPN KDVIDADLQG FGYLVPKSNK NPGKLLGVIF DSVIERNFKP LFDKLSTNPN 430 440 450 460 470 480 ALNKYTKVTA MIGGCMLNEH GVPVVPSREV TINAVKDALN NHLGISNKDL EAGQWEFTIA 490 500 510 520 530 DRCLPRFHVG YDAWQERAER KLQESYGQTV SVGGMGFSRS PGVPDVIVDG FNDALQLSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the yeast HEM14 gene coding for protoporphyrinogen oxidase, the molecular target of diphenyl ether-type herbicides." Camadro J.-M., Labbe P. J. Biol. Chem. 271:9120-9128(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Purification and properties of protoporphyrinogen oxidase from the yeast Saccharomyces cerevisiae. Mitochondrial location and evidence for a precursor form of the protein." Camadro J.-M., Thome F., Brouillet N., Labbe P. J. Biol. Chem. 269:32085-32091(1994) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: ATCC 25657 / D273-10B.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z71381 Genomic DNA. Translation: CAA95981.1. U18778 Genomic DNA. Translation: AAB64547.1. BK006939 Genomic DNA. Translation: DAA07665.1.
PIR	S50472.
RefSeq	NP_010930.1. NM_001178905.1.
3D structure databases
ProteinModelPortal	P40012.
SMR	P40012. Positions 11-281.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4483733.
STRING	4932.YER014W.
Proteomic databases
PaxDb	P40012.
PeptideAtlas	P40012.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER014W; YER014W; YER014W.
GeneID	856733.
KEGG	sce:YER014W.
Organism-specific databases
CYGD	YER014w.
SGD	S000000816. HEM14.
Phylogenomic databases
eggNOG	COG1232.
GeneTree	ENSGT00390000008744.
HOGENOM	HOG000093387.
KO	K00231.
OMA	HIRSTIN.
OrthoDB	EOG45F0ZC.
Enzyme and pathway databases
BioCyc	YEAST:YER014W-MONOMER.
SABIO-RK	P40012.
UniPathway	UPA00251; UER00324.
Gene expression databases
Genevestigator	P40012.
GermOnline	YER014W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.90.660.20. 1 hit.
InterPro	IPR004572. Protoporphyrinogen_oxidase. IPR027418. Protoporphyrinogen_oxidase_C. [Graphical view]
TIGRFAMs	TIGR00562. proto_IX_ox. 1 hit.
ProtoNet	Search...
Other
NextBio	982849.

Entry information

Entry name

PPOX_YEAST

Accession

Primary (citable) accession number: P40012
Secondary accession number(s): D3DLR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 29, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program