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Protein

Nuclear GTP-binding protein NUG1

Gene

NUG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase required for 60S ribosomal subunit export to the cytoplasm.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi213 – 2164GTPSequence Analysis
Nucleotide bindingi287 – 2948GTPSequence Analysis
Nucleotide bindingi336 – 3394GTPSequence Analysis

GO - Molecular functioni

  1. GTPase activity Source: SGD
  2. GTP binding Source: UniProtKB-KW
  3. RNA binding Source: SGD

GO - Biological processi

  1. protein transport Source: UniProtKB-KW
  2. ribosomal large subunit export from nucleus Source: SGD
  3. rRNA processing Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Ribosome biogenesis, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30193-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear GTP-binding protein NUG1
Alternative name(s):
Nuclear GTPase 1
Gene namesi
Name:NUG1
Ordered Locus Names:YER006W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome V

Organism-specific databases

CYGDiYER006w.
SGDiS000000808. NUG1.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleolus Source: SGD
  2. nucleus Source: SGD
  3. preribosome, large subunit precursor Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 520520Nuclear GTP-binding protein NUG1PRO_0000122452Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei337 – 3371Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40010.
PaxDbiP40010.
PeptideAtlasiP40010.

Expressioni

Gene expression databases

GenevestigatoriP40010.

Interactioni

Protein-protein interaction databases

BioGridi36736. 166 interactions.
DIPiDIP-6291N.
IntActiP40010. 66 interactions.
MINTiMINT-597077.
STRINGi4932.YER006W.

Structurei

3D structure databases

ProteinModelPortaliP40010.
SMRiP40010. Positions 177-448.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini165 – 343179CP-type GPROSITE-ProRule annotationAdd
BLAST

Domaini

In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.

Sequence similaritiesi

Belongs to the TRAFAC class YlqF/YawG GTPase family.PROSITE-ProRule annotation
Contains 1 CP-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1161.
GeneTreeiENSGT00550000074731.
HOGENOMiHOG000207716.
InParanoidiP40010.
KOiK14538.
OMAiPHVLEQW.
OrthoDBiEOG7X9GHD.

Family and domain databases

Gene3Di1.10.1580.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR030378. G_CP_dom.
IPR014813. Gnl3_N_dom.
IPR023179. GTP-bd_ortho_bundle.
IPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF08701. GN3L_Grn1. 1 hit.
PF01926. MMR_HSR1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51721. G_CP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40010-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVRKRQSRR TSTKLKEGIK KKASAHRKKE KKMAKKDVTW RSRSKKDPGI
60 70 80 90 100
PSNFPYKAKI LEEIEAKKMK DLEERELAKQ QRLEARKAAK EQGVDAMDED
110 120 130 140 150
MIEDDENGLA ALVESAQQAA AEYEGTPSND ADVRDDELDV IDYNIDFYGE
160 170 180 190 200
DVEGESELEK SRKAYDKIFK SVIDASDVIL YVLDARDPES TRSRKVEEAV
210 220 230 240 250
LQSQGKRLIL ILNKVDLIPP HVLEQWLNYL KSSFPTIPLR ASSGAVNGTS
260 270 280 290 300
FNRKLSQTTT ASALLESLKT YSNNSNLKRS IVVGVIGYPN VGKSSVINAL
310 320 330 340 350
LARRGGQSKA CPVGNEAGVT TSLREIKIDN KLKILDSPGI CFPSENKKRS
360 370 380 390 400
KVEHEAELAL LNALPAKHIV DPYPAVLMLV KRLAKSDEMT ESFKKLYEIP
410 420 430 440 450
PIPANDADTF TKHFLIHVAR KRGRLGKGGI PNLASAGLSV LNDWRDGKIL
460 470 480 490 500
GWVLPNTSAA ASQQDKQNLS TINTGTKQAP IAANESTIVS EWSKEFDLDG
510 520
LFSSLDKAID ASKDQDTMME
Length:520
Mass (Da):57,709
Last modified:February 1, 1995 - v1
Checksum:iF75E4860D114756A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18778 Genomic DNA. Translation: AAB64539.1.
BK006939 Genomic DNA. Translation: DAA07656.1.
PIRiS50464.
RefSeqiNP_010921.1. NM_001178897.1.

Genome annotation databases

EnsemblFungiiYER006W; YER006W; YER006W.
GeneIDi856723.
KEGGisce:YER006W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18778 Genomic DNA. Translation: AAB64539.1.
BK006939 Genomic DNA. Translation: DAA07656.1.
PIRiS50464.
RefSeqiNP_010921.1. NM_001178897.1.

3D structure databases

ProteinModelPortaliP40010.
SMRiP40010. Positions 177-448.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36736. 166 interactions.
DIPiDIP-6291N.
IntActiP40010. 66 interactions.
MINTiMINT-597077.
STRINGi4932.YER006W.

Proteomic databases

MaxQBiP40010.
PaxDbiP40010.
PeptideAtlasiP40010.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER006W; YER006W; YER006W.
GeneIDi856723.
KEGGisce:YER006W.

Organism-specific databases

CYGDiYER006w.
SGDiS000000808. NUG1.

Phylogenomic databases

eggNOGiCOG1161.
GeneTreeiENSGT00550000074731.
HOGENOMiHOG000207716.
InParanoidiP40010.
KOiK14538.
OMAiPHVLEQW.
OrthoDBiEOG7X9GHD.

Enzyme and pathway databases

BioCyciYEAST:G3O-30193-MONOMER.

Miscellaneous databases

NextBioi982820.
PROiP40010.

Gene expression databases

GenevestigatoriP40010.

Family and domain databases

Gene3Di1.10.1580.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR030378. G_CP_dom.
IPR014813. Gnl3_N_dom.
IPR023179. GTP-bd_ortho_bundle.
IPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF08701. GN3L_Grn1. 1 hit.
PF01926. MMR_HSR1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51721. G_CP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Identification of a 60S preribosomal particle that is closely linked to nuclear export."
    Bassler J., Grandi P., Gadal O., Lessmann T., Petfalski E., Tollervey D., Lechner J., Hurt E.
    Mol. Cell 8:517-529(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-337, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNUG1_YEAST
AccessioniPrimary (citable) accession number: P40010
Secondary accession number(s): D3DLQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 4, 2015
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7130 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.