##gff-version 3 P40009 UniProtKB Chain 1 630 . . . ID=PRO_0000209920;Note=Golgi apyrase P40009 UniProtKB Topological domain 1 500 . . . Note=Lumenal;Ontology_term=ECO:0000255;evidence=ECO:0000255 P40009 UniProtKB Transmembrane 501 517 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 P40009 UniProtKB Topological domain 518 630 . . . Note=Cytoplasmic;Ontology_term=ECO:0000255;evidence=ECO:0000255 P40009 UniProtKB Region 586 606 . . . Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite P40009 UniProtKB Active site 152 152 . . . Note=Proton acceptor;Ontology_term=ECO:0000250;evidence=ECO:0000250 P40009 UniProtKB Mutagenesis 152 152 . . . Note=Expressed at lower levels. GTPase activity reduced. E->Q;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16227198;Dbxref=PMID:16227198 P40009 UniProtKB Mutagenesis 189 189 . . . Note=Expressed at the same level as wild-type. GTPase and GDPase activities decreased more than 20-fold toward GTP and 3-fold for GDP. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16227198;Dbxref=PMID:16227198 P40009 UniProtKB Sequence conflict 498 498 . . . Note=S->P;Ontology_term=ECO:0000305;evidence=ECO:0000305