P40005 (PFD2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prefoldin subunit 2

Alternative name(s):
Genes involved in microtubule biogenesis protein 4
Gim complex subunit 4
Short name=GimC subunit 4

Gene names

Name:	GIM4
Synonyms:	PFD2
Ordered Locus Names:	YEL003W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	111 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.
Subunit structure	Heterohexamer of two PFD-alpha type and four PFD-beta type subunits. Ref.6
Subcellular location	Cytoplasm Ref.7.
Miscellaneous	Present with 1660 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the prefoldin subunit beta family.
Sequence caution	The sequence AAB64480.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAS56665.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Coiled coil
Molecular function	Chaperone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein folding Inferred from electronic annotation. Source: InterPro tubulin complex assembly Inferred from mutant phenotype Ref.5. Source: SGD
Cellular_component	prefoldin complex Inferred from physical interaction Ref.5. Source: SGD
Molecular_function	tubulin binding Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 111	111	Prefoldin subunit 2		PRO_0000124841
Regions
	Coiled coil	1 – 36	36	Potential
	Coiled coil	72 – 92	21	Potential
Experimental info
	Sequence conflict	59	1	A → T in ABM97488. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P40005 [UniParc]. Last modified June 26, 2007. Version 2. Checksum: E12DC1BE53C8C936	FASTA	111	12,994
	10 20 30 40 50 60 MEQRNNVFQA KYNEYKQILE ELQTKIIELG HDKDEHTIVI KTLKDAEPTR KCYRMIGGAL 70 80 90 100 110 VESDVQTSLP ILETKKENIE GTISKMKETL IQTAKEFEKW KKDNKIQVVK N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing." Juneau K., Palm C., Miranda M., Davis R.W. Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-59. Strain: ATCC 201390 / BY4743.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 7-111. Strain: ATCC 204508 / S288c.
[5]	"A novel protein complex promoting formation of functional alpha- and gamma-tubulin." Geissler S., Siegers K., Schiebel E. EMBO J. 17:952-966(1998) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Compartmentation of protein folding in vivo: sequestration of non-native polypeptide by the chaperonin-GimC system." Siegers K., Waldmann T., Leroux M.R., Grein K., Shevchenko A., Schiebel E., Hartl F.U. EMBO J. 18:75-84(1999) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE PREFOLDIN COMPLEX.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"A large-scale full-length cDNA analysis to explore the budding yeast transcriptome." Miura F., Kawaguchi N., Sese J., Toyoda A., Hattori M., Morishita S., Ito T. Proc. Natl. Acad. Sci. U.S.A. 103:17846-17851(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF INTRON.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18530 Genomic DNA. Translation: AAB64480.1. Sequence problems. EF123144 mRNA. Translation: ABM97488.1. AY558339 Genomic DNA. Translation: AAS56665.1. Sequence problems. BK006939 Genomic DNA. Translation: DAA07648.1.
PIR	S50456.
RefSeq	NP_010913.2. NM_001178818.1.
3D structure databases
ProteinModelPortal	P40005.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-7472N.
IntAct	P40005. 1 interaction.
MINT	MINT-2780320.
Proteomic databases
PaxDb	P40005.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YEL003W; YEL003W; YEL003W.
GeneID	856715.
KEGG	sce:YEL003W.
Organism-specific databases
CYGD	YEL003w.
SGD	S000000729. GIM4.
Phylogenomic databases
eggNOG	NOG331971.
GeneTree	ENSGT00390000009272.
HOGENOM	HOG000211843.
KO	K09549.
OMA	NEQVIAN.
OrthoDB	EOG447K3W.
Enzyme and pathway databases
BioCyc	YEAST:G3O-30132-MONOMER.
Gene expression databases
Genevestigator	P40005.
GermOnline	YEL003W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR027235. PFD2. IPR002777. PFD_beta-like. IPR009053. Prefoldin. [Graphical view]
PANTHER	PTHR13303. PTHR13303. 1 hit.
Pfam	PF01920. Prefoldin_2. 1 hit. [Graphical view]
SUPFAM	SSF46579. Prefoldin. 1 hit.
ProtoNet	Search...
Other
NextBio	982796.

Entry information

Entry name

PFD2_YEAST

Accession

Primary (citable) accession number: P40005
Secondary accession number(s): A2TBP1, D3DLP4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	June 26, 2007
Last modified:	May 29, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program