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P40005

- PFD2_YEAST

UniProt

P40005 - PFD2_YEAST

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Protein

Prefoldin subunit 2

Gene

GIM4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.

GO - Molecular functioni

  1. tubulin binding Source: SGD

GO - Biological processi

  1. protein folding Source: InterPro
  2. tubulin complex assembly Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Enzyme and pathway databases

BioCyciYEAST:G3O-30132-MONOMER.
ReactomeiREACT_251852. Prefoldin mediated transfer of substrate to CCT/TriC.

Names & Taxonomyi

Protein namesi
Recommended name:
Prefoldin subunit 2
Alternative name(s):
Genes involved in microtubule biogenesis protein 4
Gim complex subunit 4
Short name:
GimC subunit 4
Gene namesi
Name:GIM4
Synonyms:PFD2
Ordered Locus Names:YEL003W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYEL003w.
SGDiS000000729. GIM4.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. prefoldin complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 111111Prefoldin subunit 2PRO_0000124841Add
BLAST

Proteomic databases

MaxQBiP40005.
PaxDbiP40005.

Expressioni

Gene expression databases

GenevestigatoriP40005.

Interactioni

Subunit structurei

Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.1 Publication

Protein-protein interaction databases

BioGridi36728. 481 interactions.
DIPiDIP-7472N.
IntActiP40005. 1 interaction.
MINTiMINT-2780320.

Structurei

3D structure databases

ProteinModelPortaliP40005.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1 – 3636Sequence AnalysisAdd
BLAST
Coiled coili72 – 9221Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the prefoldin subunit beta family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG331971.
GeneTreeiENSGT00390000009272.
HOGENOMiHOG000211843.
InParanoidiP40005.
KOiK09549.
OMAiLQNQYTN.
OrthoDBiEOG7P8PN5.

Family and domain databases

Gene3Di1.10.287.370. 1 hit.
InterProiIPR027235. PFD2.
IPR002777. PFD_beta-like.
IPR009053. Prefoldin.
[Graphical view]
PANTHERiPTHR13303. PTHR13303. 1 hit.
PfamiPF01920. Prefoldin_2. 1 hit.
[Graphical view]
SUPFAMiSSF46579. SSF46579. 1 hit.

Sequencei

Sequence statusi: Complete.

P40005-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEQRNNVFQA KYNEYKQILE ELQTKIIELG HDKDEHTIVI KTLKDAEPTR
60 70 80 90 100
KCYRMIGGAL VESDVQTSLP ILETKKENIE GTISKMKETL IQTAKEFEKW
110
KKDNKIQVVK N
Length:111
Mass (Da):12,994
Last modified:June 26, 2007 - v2
Checksum:iE12DC1BE53C8C936
GO

Sequence cautioni

The sequence AAB64480.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAS56665.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti59 – 591A → T in ABM97488. (PubMed:17244705)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18530 Genomic DNA. Translation: AAB64480.1. Sequence problems.
EF123144 mRNA. Translation: ABM97488.1.
AY558339 Genomic DNA. Translation: AAS56665.1. Sequence problems.
BK006939 Genomic DNA. Translation: DAA07648.1.
PIRiS50456.
RefSeqiNP_010913.2. NM_001178818.1.

Genome annotation databases

EnsemblFungiiYEL003W; YEL003W; YEL003W.
GeneIDi856715.
KEGGisce:YEL003W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18530 Genomic DNA. Translation: AAB64480.1 . Sequence problems.
EF123144 mRNA. Translation: ABM97488.1 .
AY558339 Genomic DNA. Translation: AAS56665.1 . Sequence problems.
BK006939 Genomic DNA. Translation: DAA07648.1 .
PIRi S50456.
RefSeqi NP_010913.2. NM_001178818.1.

3D structure databases

ProteinModelPortali P40005.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36728. 481 interactions.
DIPi DIP-7472N.
IntActi P40005. 1 interaction.
MINTi MINT-2780320.

Proteomic databases

MaxQBi P40005.
PaxDbi P40005.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YEL003W ; YEL003W ; YEL003W .
GeneIDi 856715.
KEGGi sce:YEL003W.

Organism-specific databases

CYGDi YEL003w.
SGDi S000000729. GIM4.

Phylogenomic databases

eggNOGi NOG331971.
GeneTreei ENSGT00390000009272.
HOGENOMi HOG000211843.
InParanoidi P40005.
KOi K09549.
OMAi LQNQYTN.
OrthoDBi EOG7P8PN5.

Enzyme and pathway databases

BioCyci YEAST:G3O-30132-MONOMER.
Reactomei REACT_251852. Prefoldin mediated transfer of substrate to CCT/TriC.

Miscellaneous databases

NextBioi 982796.
PROi P40005.

Gene expression databases

Genevestigatori P40005.

Family and domain databases

Gene3Di 1.10.287.370. 1 hit.
InterProi IPR027235. PFD2.
IPR002777. PFD_beta-like.
IPR009053. Prefoldin.
[Graphical view ]
PANTHERi PTHR13303. PTHR13303. 1 hit.
Pfami PF01920. Prefoldin_2. 1 hit.
[Graphical view ]
SUPFAMi SSF46579. SSF46579. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing."
    Juneau K., Palm C., Miranda M., Davis R.W.
    Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-59.
    Strain: ATCC 201390 / BY4743.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 7-111.
    Strain: ATCC 204508 / S288c.
  5. "A novel protein complex promoting formation of functional alpha- and gamma-tubulin."
    Geissler S., Siegers K., Schiebel E.
    EMBO J. 17:952-966(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "Compartmentation of protein folding in vivo: sequestration of non-native polypeptide by the chaperonin-GimC system."
    Siegers K., Waldmann T., Leroux M.R., Grein K., Shevchenko A., Schiebel E., Hartl F.U.
    EMBO J. 18:75-84(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE PREFOLDIN COMPLEX.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "A large-scale full-length cDNA analysis to explore the budding yeast transcriptome."
    Miura F., Kawaguchi N., Sese J., Toyoda A., Hattori M., Morishita S., Ito T.
    Proc. Natl. Acad. Sci. U.S.A. 103:17846-17851(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF INTRON.

Entry informationi

Entry nameiPFD2_YEAST
AccessioniPrimary (citable) accession number: P40005
Secondary accession number(s): A2TBP1, D3DLP4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: June 26, 2007
Last modified: November 26, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1660 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3