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Protein

Prefoldin subunit 2

Gene

GIM4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.

GO - Molecular functioni

  1. tubulin binding Source: SGD

GO - Biological processi

  1. protein folding Source: InterPro
  2. tubulin complex assembly Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Enzyme and pathway databases

BioCyciYEAST:G3O-30132-MONOMER.
ReactomeiREACT_306488. Prefoldin mediated transfer of substrate to CCT/TriC.

Names & Taxonomyi

Protein namesi
Recommended name:
Prefoldin subunit 2
Alternative name(s):
Genes involved in microtubule biogenesis protein 4
Gim complex subunit 4
Short name:
GimC subunit 4
Gene namesi
Name:GIM4
Synonyms:PFD2
Ordered Locus Names:YEL003W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome V

Organism-specific databases

CYGDiYEL003w.
SGDiS000000729. GIM4.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. prefoldin complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 111111Prefoldin subunit 2PRO_0000124841Add
BLAST

Proteomic databases

MaxQBiP40005.
PaxDbiP40005.

Expressioni

Gene expression databases

GenevestigatoriP40005.

Interactioni

Subunit structurei

Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.1 Publication

Protein-protein interaction databases

BioGridi36728. 481 interactions.
DIPiDIP-7472N.
IntActiP40005. 1 interaction.
MINTiMINT-2780320.

Structurei

3D structure databases

ProteinModelPortaliP40005.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1 – 3636Sequence AnalysisAdd
BLAST
Coiled coili72 – 9221Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the prefoldin subunit beta family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG331971.
GeneTreeiENSGT00390000009272.
HOGENOMiHOG000211843.
InParanoidiP40005.
KOiK09549.
OMAiKCFRMVN.
OrthoDBiEOG7P8PN5.

Family and domain databases

Gene3Di1.10.287.370. 1 hit.
InterProiIPR027235. PFD2.
IPR002777. PFD_beta-like.
IPR009053. Prefoldin.
[Graphical view]
PANTHERiPTHR13303. PTHR13303. 1 hit.
PfamiPF01920. Prefoldin_2. 1 hit.
[Graphical view]
SUPFAMiSSF46579. SSF46579. 1 hit.

Sequencei

Sequence statusi: Complete.

P40005-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQRNNVFQA KYNEYKQILE ELQTKIIELG HDKDEHTIVI KTLKDAEPTR
60 70 80 90 100
KCYRMIGGAL VESDVQTSLP ILETKKENIE GTISKMKETL IQTAKEFEKW
110
KKDNKIQVVK N
Length:111
Mass (Da):12,994
Last modified:June 25, 2007 - v2
Checksum:iE12DC1BE53C8C936
GO

Sequence cautioni

The sequence AAB64480.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAS56665.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti59 – 591A → T in ABM97488 (PubMed:17244705).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18530 Genomic DNA. Translation: AAB64480.1. Sequence problems.
EF123144 mRNA. Translation: ABM97488.1.
AY558339 Genomic DNA. Translation: AAS56665.1. Sequence problems.
BK006939 Genomic DNA. Translation: DAA07648.1.
PIRiS50456.
RefSeqiNP_010913.2. NM_001178818.1.

Genome annotation databases

EnsemblFungiiYEL003W; YEL003W; YEL003W.
GeneIDi856715.
KEGGisce:YEL003W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18530 Genomic DNA. Translation: AAB64480.1. Sequence problems.
EF123144 mRNA. Translation: ABM97488.1.
AY558339 Genomic DNA. Translation: AAS56665.1. Sequence problems.
BK006939 Genomic DNA. Translation: DAA07648.1.
PIRiS50456.
RefSeqiNP_010913.2. NM_001178818.1.

3D structure databases

ProteinModelPortaliP40005.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36728. 481 interactions.
DIPiDIP-7472N.
IntActiP40005. 1 interaction.
MINTiMINT-2780320.

Proteomic databases

MaxQBiP40005.
PaxDbiP40005.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYEL003W; YEL003W; YEL003W.
GeneIDi856715.
KEGGisce:YEL003W.

Organism-specific databases

CYGDiYEL003w.
SGDiS000000729. GIM4.

Phylogenomic databases

eggNOGiNOG331971.
GeneTreeiENSGT00390000009272.
HOGENOMiHOG000211843.
InParanoidiP40005.
KOiK09549.
OMAiKCFRMVN.
OrthoDBiEOG7P8PN5.

Enzyme and pathway databases

BioCyciYEAST:G3O-30132-MONOMER.
ReactomeiREACT_306488. Prefoldin mediated transfer of substrate to CCT/TriC.

Miscellaneous databases

NextBioi982796.
PROiP40005.

Gene expression databases

GenevestigatoriP40005.

Family and domain databases

Gene3Di1.10.287.370. 1 hit.
InterProiIPR027235. PFD2.
IPR002777. PFD_beta-like.
IPR009053. Prefoldin.
[Graphical view]
PANTHERiPTHR13303. PTHR13303. 1 hit.
PfamiPF01920. Prefoldin_2. 1 hit.
[Graphical view]
SUPFAMiSSF46579. SSF46579. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing."
    Juneau K., Palm C., Miranda M., Davis R.W.
    Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-59.
    Strain: ATCC 201390 / BY4743.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 7-111.
    Strain: ATCC 204508 / S288c.
  5. "A novel protein complex promoting formation of functional alpha- and gamma-tubulin."
    Geissler S., Siegers K., Schiebel E.
    EMBO J. 17:952-966(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "Compartmentation of protein folding in vivo: sequestration of non-native polypeptide by the chaperonin-GimC system."
    Siegers K., Waldmann T., Leroux M.R., Grein K., Shevchenko A., Schiebel E., Hartl F.U.
    EMBO J. 18:75-84(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE PREFOLDIN COMPLEX.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "A large-scale full-length cDNA analysis to explore the budding yeast transcriptome."
    Miura F., Kawaguchi N., Sese J., Toyoda A., Hattori M., Morishita S., Ito T.
    Proc. Natl. Acad. Sci. U.S.A. 103:17846-17851(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF INTRON.

Entry informationi

Entry nameiPFD2_YEAST
AccessioniPrimary (citable) accession number: P40005
Secondary accession number(s): A2TBP1, D3DLP4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 1995
Last sequence update: June 25, 2007
Last modified: March 31, 2015
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1660 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.