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P40005 (PFD2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Prefoldin subunit 2
Alternative name(s):
Genes involved in microtubule biogenesis protein 4
Gim complex subunit 4
Short name=GimC subunit 4
Gene names
Name:GIM4
Synonyms:PFD2
Ordered Locus Names:YEL003W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length111 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.

Subunit structure

Heterohexamer of two PFD-alpha type and four PFD-beta type subunits. Ref.6

Subcellular location

Cytoplasm Ref.7.

Miscellaneous

Present with 1660 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the prefoldin subunit beta family.

Sequence caution

The sequence AAB64480.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence AAS56665.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Cellular componentCytoplasm
   DomainCoiled coil
   Molecular functionChaperone
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein folding

Inferred from electronic annotation. Source: InterPro

tubulin complex assembly

Inferred from mutant phenotype Ref.5. Source: SGD

   Cellular_componentcytoplasm

Inferred from direct assay Ref.5. Source: SGD

prefoldin complex

Inferred from physical interaction Ref.5. Source: SGD

   Molecular_functiontubulin binding

Inferred from direct assay Ref.5. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 111111Prefoldin subunit 2
PRO_0000124841

Regions

Coiled coil1 – 3636 Potential
Coiled coil72 – 9221 Potential

Experimental info

Sequence conflict591A → T in ABM97488. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P40005 [UniParc].

Last modified June 26, 2007. Version 2.
Checksum: E12DC1BE53C8C936

FASTA11112,994
        10         20         30         40         50         60 
MEQRNNVFQA KYNEYKQILE ELQTKIIELG HDKDEHTIVI KTLKDAEPTR KCYRMIGGAL 

        70         80         90        100        110 
VESDVQTSLP ILETKKENIE GTISKMKETL IQTAKEFEKW KKDNKIQVVK N 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing."
Juneau K., Palm C., Miranda M., Davis R.W.
Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-59.
Strain: ATCC 201390 / BY4743.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 7-111.
Strain: ATCC 204508 / S288c.
[5]"A novel protein complex promoting formation of functional alpha- and gamma-tubulin."
Geissler S., Siegers K., Schiebel E.
EMBO J. 17:952-966(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"Compartmentation of protein folding in vivo: sequestration of non-native polypeptide by the chaperonin-GimC system."
Siegers K., Waldmann T., Leroux M.R., Grein K., Shevchenko A., Schiebel E., Hartl F.U.
EMBO J. 18:75-84(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE PREFOLDIN COMPLEX.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"A large-scale full-length cDNA analysis to explore the budding yeast transcriptome."
Miura F., Kawaguchi N., Sese J., Toyoda A., Hattori M., Morishita S., Ito T.
Proc. Natl. Acad. Sci. U.S.A. 103:17846-17851(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF INTRON.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U18530 Genomic DNA. Translation: AAB64480.1. Sequence problems.
EF123144 mRNA. Translation: ABM97488.1.
AY558339 Genomic DNA. Translation: AAS56665.1. Sequence problems.
BK006939 Genomic DNA. Translation: DAA07648.1.
PIRS50456.
RefSeqNP_010913.2. NM_001178818.1.

3D structure databases

ProteinModelPortalP40005.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36728. 481 interactions.
DIPDIP-7472N.
IntActP40005. 1 interaction.
MINTMINT-2780320.

Proteomic databases

PaxDbP40005.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYEL003W; YEL003W; YEL003W.
GeneID856715.
KEGGsce:YEL003W.

Organism-specific databases

CYGDYEL003w.
SGDS000000729. GIM4.

Phylogenomic databases

eggNOGNOG331971.
GeneTreeENSGT00390000009272.
HOGENOMHOG000211843.
KOK09549.
OMAKNTLTQM.
OrthoDBEOG7P8PN5.

Enzyme and pathway databases

BioCycYEAST:G3O-30132-MONOMER.

Gene expression databases

GenevestigatorP40005.

Family and domain databases

Gene3D1.10.287.370. 1 hit.
InterProIPR027235. PFD2.
IPR002777. PFD_beta-like.
IPR009053. Prefoldin.
[Graphical view]
PANTHERPTHR13303. PTHR13303. 1 hit.
PfamPF01920. Prefoldin_2. 1 hit.
[Graphical view]
SUPFAMSSF46579. SSF46579. 1 hit.
ProtoNetSearch...

Other

NextBio982796.
PROP40005.

Entry information

Entry namePFD2_YEAST
AccessionPrimary (citable) accession number: P40005
Secondary accession number(s): A2TBP1, D3DLP4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: June 26, 2007
Last modified: April 16, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families