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P40005

- PFD2_YEAST

UniProt

P40005 - PFD2_YEAST

Protein

Prefoldin subunit 2

Gene

GIM4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.

    GO - Molecular functioni

    1. tubulin binding Source: SGD

    GO - Biological processi

    1. protein folding Source: InterPro
    2. tubulin complex assembly Source: SGD

    Keywords - Molecular functioni

    Chaperone

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30132-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Prefoldin subunit 2
    Alternative name(s):
    Genes involved in microtubule biogenesis protein 4
    Gim complex subunit 4
    Short name:
    GimC subunit 4
    Gene namesi
    Name:GIM4
    Synonyms:PFD2
    Ordered Locus Names:YEL003W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYEL003w.
    SGDiS000000729. GIM4.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. prefoldin complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 111111Prefoldin subunit 2PRO_0000124841Add
    BLAST

    Proteomic databases

    MaxQBiP40005.
    PaxDbiP40005.

    Expressioni

    Gene expression databases

    GenevestigatoriP40005.

    Interactioni

    Subunit structurei

    Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.1 Publication

    Protein-protein interaction databases

    BioGridi36728. 481 interactions.
    DIPiDIP-7472N.
    IntActiP40005. 1 interaction.
    MINTiMINT-2780320.

    Structurei

    3D structure databases

    ProteinModelPortaliP40005.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili1 – 3636Sequence AnalysisAdd
    BLAST
    Coiled coili72 – 9221Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prefoldin subunit beta family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG331971.
    GeneTreeiENSGT00390000009272.
    HOGENOMiHOG000211843.
    KOiK09549.
    OMAiLQNQYTN.
    OrthoDBiEOG7P8PN5.

    Family and domain databases

    Gene3Di1.10.287.370. 1 hit.
    InterProiIPR027235. PFD2.
    IPR002777. PFD_beta-like.
    IPR009053. Prefoldin.
    [Graphical view]
    PANTHERiPTHR13303. PTHR13303. 1 hit.
    PfamiPF01920. Prefoldin_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF46579. SSF46579. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P40005-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEQRNNVFQA KYNEYKQILE ELQTKIIELG HDKDEHTIVI KTLKDAEPTR    50
    KCYRMIGGAL VESDVQTSLP ILETKKENIE GTISKMKETL IQTAKEFEKW 100
    KKDNKIQVVK N 111
    Length:111
    Mass (Da):12,994
    Last modified:June 26, 2007 - v2
    Checksum:iE12DC1BE53C8C936
    GO

    Sequence cautioni

    The sequence AAB64480.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence AAS56665.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti59 – 591A → T in ABM97488. (PubMed:17244705)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18530 Genomic DNA. Translation: AAB64480.1. Sequence problems.
    EF123144 mRNA. Translation: ABM97488.1.
    AY558339 Genomic DNA. Translation: AAS56665.1. Sequence problems.
    BK006939 Genomic DNA. Translation: DAA07648.1.
    PIRiS50456.
    RefSeqiNP_010913.2. NM_001178818.1.

    Genome annotation databases

    EnsemblFungiiYEL003W; YEL003W; YEL003W.
    GeneIDi856715.
    KEGGisce:YEL003W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18530 Genomic DNA. Translation: AAB64480.1 . Sequence problems.
    EF123144 mRNA. Translation: ABM97488.1 .
    AY558339 Genomic DNA. Translation: AAS56665.1 . Sequence problems.
    BK006939 Genomic DNA. Translation: DAA07648.1 .
    PIRi S50456.
    RefSeqi NP_010913.2. NM_001178818.1.

    3D structure databases

    ProteinModelPortali P40005.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36728. 481 interactions.
    DIPi DIP-7472N.
    IntActi P40005. 1 interaction.
    MINTi MINT-2780320.

    Proteomic databases

    MaxQBi P40005.
    PaxDbi P40005.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YEL003W ; YEL003W ; YEL003W .
    GeneIDi 856715.
    KEGGi sce:YEL003W.

    Organism-specific databases

    CYGDi YEL003w.
    SGDi S000000729. GIM4.

    Phylogenomic databases

    eggNOGi NOG331971.
    GeneTreei ENSGT00390000009272.
    HOGENOMi HOG000211843.
    KOi K09549.
    OMAi LQNQYTN.
    OrthoDBi EOG7P8PN5.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30132-MONOMER.

    Miscellaneous databases

    NextBioi 982796.
    PROi P40005.

    Gene expression databases

    Genevestigatori P40005.

    Family and domain databases

    Gene3Di 1.10.287.370. 1 hit.
    InterProi IPR027235. PFD2.
    IPR002777. PFD_beta-like.
    IPR009053. Prefoldin.
    [Graphical view ]
    PANTHERi PTHR13303. PTHR13303. 1 hit.
    Pfami PF01920. Prefoldin_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46579. SSF46579. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing."
      Juneau K., Palm C., Miranda M., Davis R.W.
      Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-59.
      Strain: ATCC 201390 / BY4743.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 7-111.
      Strain: ATCC 204508 / S288c.
    5. "A novel protein complex promoting formation of functional alpha- and gamma-tubulin."
      Geissler S., Siegers K., Schiebel E.
      EMBO J. 17:952-966(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. "Compartmentation of protein folding in vivo: sequestration of non-native polypeptide by the chaperonin-GimC system."
      Siegers K., Waldmann T., Leroux M.R., Grein K., Shevchenko A., Schiebel E., Hartl F.U.
      EMBO J. 18:75-84(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE PREFOLDIN COMPLEX.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "A large-scale full-length cDNA analysis to explore the budding yeast transcriptome."
      Miura F., Kawaguchi N., Sese J., Toyoda A., Hattori M., Morishita S., Ito T.
      Proc. Natl. Acad. Sci. U.S.A. 103:17846-17851(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF INTRON.

    Entry informationi

    Entry nameiPFD2_YEAST
    AccessioniPrimary (citable) accession number: P40005
    Secondary accession number(s): A2TBP1, D3DLP4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: June 26, 2007
    Last modified: October 1, 2014
    This is version 97 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1660 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3