P40003 (VAB2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Biogenesis of lysosome-related organelles complex 1 subunit VAB2
Short name=BLOC-1 subunit VAB2

Alternative name(s):
VAC8-binding protein 2

Gene names

Name:	VAB2
Synonyms:	VAB31
Ordered Locus Names:	YEL005C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the biogenesis of lysosome-related organelles complex-1 (BLOC-1) involved in endosomal cargo sorting. Ref.5
Subunit structure	Component of the biogenesis of lysosome-related organelles complex-1 (BLOC-1) composed of at least BLI1, BLS1, CNL1, KXD1, SNN1 and VAB2. Interacts with VAC8. Ref.3
Subcellular location	Cytoplasmic vesicle. Cytoplasm. Vacuole Ref.4.
Sequence similarities	Belongs to the VAB2 family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cytoplasm Cytoplasmic vesicle Vacuole
Domain	Coiled coil
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasmic membrane-bounded vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction Ref.3. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
VAC8	P39968	2	EBI-22275,EBI-20212

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	Biogenesis of lysosome-related organelles complex 1 subunit VAB2		PRO_0000202618
Regions
	Coiled coil	64 – 140	77	Potential

Sequences

	Sequence		Length	Mass (Da)
	P40003 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 8F7CA122F91E491A	FASTA	282	31,365
	10 20 30 40 50 60 MVADLTKGIL KWKSRIEFDA VGSSSYYEEL KGLPPLASHK KLTQAAIFNS TKYELLQVKK 70 80 90 100 110 120 DILSIYEVVS RDIDEERNQM QQIELQLKKS LKKVEHSYKN VLKQRASTNC INGNDRLLAN 130 140 150 160 170 180 AEKKIGSLNE ELACVNDIVS DIVNNLTALN ANLPKKAQLL KDDSINVAHY PLLFDFLHKS 190 200 210 220 230 240 CPKSIIATSE ASIHENASPS PLLEHDELPA ESINSFYGEN ELQSDSLAPL QTHDDNISSC 250 260 270 280 KKILPPKFNT TSGPSIETNF ENISADGLTY TKCSLKNSIS LT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Nucleus-vacuole junctions in Saccharomyces cerevisiae are formed through the direct interaction of Vac8p with Nvj1p." Pan X., Roberts P., Chen Y., Kvam E., Shulga N., Huang K., Lemmon S., Goldfarb D.S. Mol. Biol. Cell 11:2445-2457(2000) [PubMed: 10888680] [Abstract] Cited for: INTERACTION WITH VAC8.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Yeast homologues of three BLOC-1 subunits highlight KxDL proteins as conserved interactors of BLOC-1." Hayes M.J., Bryon K., Satkurunathan J., Levine T.P. Traffic 12:260-268(2011) [PubMed: 21159114] [Abstract] Cited for: IDENTIFICATION IN THE BLOC-1 COMPLEX, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18530 Genomic DNA. Translation: AAB64482.1. BK006939 Genomic DNA. Translation: DAA07646.1.
PIR	S50454.
RefSeq	NP_010911.1. NM_001178820.1.
3D structure databases
ProteinModelPortal	P40003.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5277N.
IntAct	P40003. 3 interactions.
MINT	MINT-498661.
STRING	P40003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YEL005C; YEL005C; YEL005C.
GeneID	856713.
KEGG	sce:YEL005C.
NMPDR	fig|4932.3.peg.1969.
Organism-specific databases
CYGD	YEL005c.
SGD	S000000731. VAB2.
Phylogenomic databases
eggNOG	fuNOG12946.
OMA	ASIHENA.
OrthoDB	EOG4HHSC6.
Gene expression databases
ArrayExpress	P40003.
Genevestigator	P40003.
GermOnline	YEL005C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	982790.

Entry information

Entry name

VAB2_YEAST

Accession

Primary (citable) accession number: P40003
Secondary accession number(s): D3DLP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program