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Protein

Enhancer of mRNA-decapping protein 3

Gene

EDC3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Stimulates decapping of both stable and unstable mRNA during mRNA decay. Does not affect nonsense-mediated mRNA decay. Required for normal P-body assembly.2 Publications

Miscellaneous

Present with 2340 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • mRNA binding Source: SGD

GO - Biological processi

  • cytoplasmic mRNA processing body assembly Source: SGD
  • deadenylation-independent decapping of nuclear-transcribed mRNA Source: SGD
  • positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: SGD

Enzyme and pathway databases

BioCyciYEAST:G3O-30140-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Enhancer of mRNA-decapping protein 3
Gene namesi
Name:EDC3
Synonyms:LSM16
Ordered Locus Names:YEL015W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL015W.
SGDiS000000741. EDC3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi360 – 361RH → AA: Abolishes homodimerization. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002026131 – 551Enhancer of mRNA-decapping protein 3Add BLAST551

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei257PhosphoserineCombined sources1
Modified residuei261PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39998.
PRIDEiP39998.

PTM databases

iPTMnetiP39998.

Interactioni

Subunit structurei

Homodimer. Interacts with DCP2.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36716. 79 interactors.
DIPiDIP-842N.
IntActiP39998. 24 interactors.
MINTiMINT-385078.
STRINGi4932.YEL015W.

Structurei

Secondary structure

1551
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi91 – 94Combined sources4
Turni97 – 99Combined sources3
Helixi107 – 110Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4BRUX-ray3.24B77-116[»]
ProteinModelPortaliP39998.
SMRiP39998.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini93 – 129DFDFAdd BLAST37
Domaini288 – 527YjeF N-terminalAdd BLAST240

Sequence similaritiesi

Belongs to the EDC3 family.Curated

Phylogenomic databases

HOGENOMiHOG000246503.
InParanoidiP39998.
KOiK12615.
OMAiGYDDHLE.
OrthoDBiEOG092C25XS.

Family and domain databases

Gene3Di3.40.50.10260. 1 hit.
InterProiView protein in InterPro
IPR025762. DFDF.
IPR019050. FDF_dom.
IPR004443. YjeF_N_dom.
IPR036652. YjeF_N_dom_sf.
PfamiView protein in Pfam
PF09532. FDF. 1 hit.
PF03853. YjeF_N. 1 hit.
SMARTiView protein in SMART
SM01199. FDF. 1 hit.
SUPFAMiSSF64153. SSF64153. 1 hit.
PROSITEiView protein in PROSITE
PS51512. DFDF. 1 hit.
PS51385. YJEF_N. 1 hit.

Sequencei

Sequence statusi: Complete.

P39998-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQFVGFGVQ VELKDGKLIQ GKIAKATSKG LTLNDVQFGD GGKSQAFKVR
60 70 80 90 100
ASRLKDLKVL TVASQSGKRK QQRQQQQQND YNQNRGEHID WQDDDVSKIK
110 120 130 140 150
QQEDFDFQRN LGMFNKKDVF AQLKQNDDIL PENRLQGHNR KQTQLQQNNY
160 170 180 190 200
QNDELVIPDA KKDSWNKISS RNEQSTHQSQ PQQDAQDDLV LEDDEHEYDV
210 220 230 240 250
DDIDDPKYLP ITQSLNITHL IHSATNSPSI NDKTKGTVIN DKDQVLAKLG
260 270 280 290 300
QMIISQSRSN STSLPAANKQ TTIRSKNTKQ NIPMATPVQL LEMESITSEF
310 320 330 340 350
FSINSAGLLE NFAVNASFFL KQKLGGRARL RLQNSNPEPL VVILASDSNR
360 370 380 390 400
SGAKALALGR HLCQTGHIRV ITLFTCSQNE LQDSMVKKQT DIYKKCGGKI
410 420 430 440 450
VNSVSSLESA METLNSPVEI VIDAMQGYDC TLSDLAGTSE VIESRIKSMI
460 470 480 490 500
SWCNKQRGST KVWSLDIPNG FDAGSGMPDI FFSDRIEATG IICSGWPLIA
510 520 530 540 550
INNLIANLPS LEDAVLIDIG IPQGAYSQRT SLRKFQNCDL FVTDGSLLLD

L
Length:551
Mass (Da):61,340
Last modified:February 1, 1995 - v1
Checksum:iBF28AE67F9303A73
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18530 Genomic DNA. Translation: AAB64492.1.
BK006939 Genomic DNA. Translation: DAA07639.1.
PIRiS50444.
RefSeqiNP_010901.1. NM_001178830.1.

Genome annotation databases

EnsemblFungiiYEL015W; YEL015W; YEL015W.
GeneIDi856700.
KEGGisce:YEL015W.

Similar proteinsi

Entry informationi

Entry nameiEDC3_YEAST
AccessioniPrimary (citable) accession number: P39998
Secondary accession number(s): D3DLN5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 25, 2017
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names