Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Enhancer of mRNA-decapping protein 3

Gene

EDC3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Stimulates decapping of both stable and unstable mRNA during mRNA decay. Does not affect nonsense-mediated mRNA decay. Required for normal P-body assembly.2 Publications

Miscellaneous

Present with 2340 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • mRNA binding Source: SGD

GO - Biological processi

  • cytoplasmic mRNA processing body assembly Source: SGD
  • deadenylation-independent decapping of nuclear-transcribed mRNA Source: SGD
  • positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: SGD

Enzyme and pathway databases

BioCyciYEAST:G3O-30140-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Enhancer of mRNA-decapping protein 3
Gene namesi
Name:EDC3
Synonyms:LSM16
Ordered Locus Names:YEL015W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL015W
SGDiS000000741 EDC3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi360 – 361RH → AA: Abolishes homodimerization. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002026131 – 551Enhancer of mRNA-decapping protein 3Add BLAST551

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei257PhosphoserineCombined sources1
Modified residuei261PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39998
PaxDbiP39998
PRIDEiP39998

PTM databases

iPTMnetiP39998

Interactioni

Subunit structurei

Homodimer. Interacts with DCP2.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36716, 79 interactors
DIPiDIP-842N
IntActiP39998, 32 interactors
MINTiP39998
STRINGi4932.YEL015W

Structurei

Secondary structure

1551
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi91 – 94Combined sources4
Turni97 – 99Combined sources3
Helixi107 – 110Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4BRUX-ray3.24B77-116[»]
ProteinModelPortaliP39998
SMRiP39998
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini93 – 129DFDFPROSITE-ProRule annotationAdd BLAST37
Domaini288 – 527YjeF N-terminalPROSITE-ProRule annotationAdd BLAST240

Sequence similaritiesi

Belongs to the EDC3 family.Curated

Phylogenomic databases

HOGENOMiHOG000246503
InParanoidiP39998
KOiK12615
OMAiAMFDKKS
OrthoDBiEOG092C25XS

Family and domain databases

Gene3Di3.40.50.10260, 1 hit
InterProiView protein in InterPro
IPR025762 DFDF
IPR019050 FDF_dom
IPR004443 YjeF_N_dom
IPR036652 YjeF_N_dom_sf
PfamiView protein in Pfam
PF09532 FDF, 1 hit
PF03853 YjeF_N, 1 hit
SMARTiView protein in SMART
SM01199 FDF, 1 hit
SUPFAMiSSF64153 SSF64153, 1 hit
PROSITEiView protein in PROSITE
PS51512 DFDF, 1 hit
PS51385 YJEF_N, 1 hit

Sequencei

Sequence statusi: Complete.

P39998-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQFVGFGVQ VELKDGKLIQ GKIAKATSKG LTLNDVQFGD GGKSQAFKVR
60 70 80 90 100
ASRLKDLKVL TVASQSGKRK QQRQQQQQND YNQNRGEHID WQDDDVSKIK
110 120 130 140 150
QQEDFDFQRN LGMFNKKDVF AQLKQNDDIL PENRLQGHNR KQTQLQQNNY
160 170 180 190 200
QNDELVIPDA KKDSWNKISS RNEQSTHQSQ PQQDAQDDLV LEDDEHEYDV
210 220 230 240 250
DDIDDPKYLP ITQSLNITHL IHSATNSPSI NDKTKGTVIN DKDQVLAKLG
260 270 280 290 300
QMIISQSRSN STSLPAANKQ TTIRSKNTKQ NIPMATPVQL LEMESITSEF
310 320 330 340 350
FSINSAGLLE NFAVNASFFL KQKLGGRARL RLQNSNPEPL VVILASDSNR
360 370 380 390 400
SGAKALALGR HLCQTGHIRV ITLFTCSQNE LQDSMVKKQT DIYKKCGGKI
410 420 430 440 450
VNSVSSLESA METLNSPVEI VIDAMQGYDC TLSDLAGTSE VIESRIKSMI
460 470 480 490 500
SWCNKQRGST KVWSLDIPNG FDAGSGMPDI FFSDRIEATG IICSGWPLIA
510 520 530 540 550
INNLIANLPS LEDAVLIDIG IPQGAYSQRT SLRKFQNCDL FVTDGSLLLD

L
Length:551
Mass (Da):61,340
Last modified:February 1, 1995 - v1
Checksum:iBF28AE67F9303A73
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18530 Genomic DNA Translation: AAB64492.1
BK006939 Genomic DNA Translation: DAA07639.1
PIRiS50444
RefSeqiNP_010901.1, NM_001178830.1

Genome annotation databases

EnsemblFungiiYEL015W; YEL015W; YEL015W
GeneIDi856700
KEGGisce:YEL015W

Similar proteinsi

Entry informationi

Entry nameiEDC3_YEAST
AccessioniPrimary (citable) accession number: P39998
Secondary accession number(s): D3DLN5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 28, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health