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P39997

- NPP2_YEAST

UniProt

P39997 - NPP2_YEAST

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Protein

Ectonucleotide pyrophosphatase/phosphodiesterase 2

Gene

NPP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Mediates extracellular nucleotide derived phosphate hydrolysis along with NPP1 and PHO5.1 Publication

Catalytic activityi

Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides.
A dinucleotide + H2O = 2 mononucleotides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei127 – 1271NucleophileBy similarity

GO - Molecular functioni

  1. NADH pyrophosphatase activity Source: UniProtKB-EC
  2. nucleoside-triphosphatase activity Source: SGD
  3. nucleoside-triphosphate diphosphatase activity Source: SGD
  4. nucleotide diphosphatase activity Source: SGD
  5. phosphodiesterase I activity Source: SGD

GO - Biological processi

  1. nucleic acid phosphodiester bond hydrolysis Source: GOC
  2. nucleoside triphosphate metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciYEAST:G3O-30141-MONOMER.
ReactomeiREACT_188975. Glycosphingolipid metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Ectonucleotide pyrophosphatase/phosphodiesterase 2
Short name:
E-NPP 2
Including the following 2 domains:
Alkaline phosphodiesterase 1 (EC:3.1.4.1)
Nucleotide pyrophosphatase (EC:3.6.1.9)
Short name:
NPPase
Gene namesi
Name:NPP2
Ordered Locus Names:YEL016C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYEL016c.
SGDiS000000742. NPP2.

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 493493Ectonucleotide pyrophosphatase/phosphodiesterase 2PRO_0000202612Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi62 – 621N-linked (GlcNAc...)Sequence Analysis
Glycosylationi69 – 691N-linked (GlcNAc...)Sequence Analysis
Glycosylationi112 – 1121N-linked (GlcNAc...)Sequence Analysis
Glycosylationi153 – 1531N-linked (GlcNAc...)Sequence Analysis
Glycosylationi441 – 4411N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Autophosphorylated as part of the catalytic cycle of phosphodiesterase/pyrophosphatase activity.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP39997.

Expressioni

Inductioni

Up-regulated during phosphate starvation.1 Publication

Gene expression databases

GenevestigatoriP39997.

Interactioni

Protein-protein interaction databases

BioGridi36715. 10 interactions.
DIPiDIP-5279N.
IntActiP39997. 1 interaction.
MINTiMINT-511815.
STRINGi4932.YEL016C.

Structurei

3D structure databases

ProteinModelPortaliP39997.
SMRiP39997. Positions 79-457.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2828CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini46 – 493448ExtracellularSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei29 – 4517Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni76 – 438363PhosphodiesteraseAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1524.
GeneTreeiENSGT00760000119157.
InParanoidiP39997.
OMAiYDAFRTE.
OrthoDBiEOG74J9HK.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024873. E-NPP.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
PANTHERiPTHR10151. PTHR10151. 1 hit.
PfamiPF01663. Phosphodiest. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.

Sequencei

Sequence statusi: Complete.

P39997-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLLFEQPVDL EKNNEDDTNI KPFAISRHFL LKLLLCGIIL IELLLYSKCP
60 70 80 90 100
KPIDNGPRTI ANRSNTYFNG THDFKTLTIL ISIDGFHPRL IDAKYTPFLY
110 120 130 140 150
NLHNLRSPYD MNITTAPYMI PSFPTQTFPN HWSMVTGKYP IEHGIVSNIF
160 170 180 190 200
WDNFTSSEFR PNNLDARIWS NTADPIWQLL QTESQGEYKV ATHMWPGSEV
210 220 230 240 250
VYEDHGDVPR ERMPFYFGKF NQWEKLQDKL AQIFRYIDMP QLKDRPELVI
260 270 280 290 300
SYIPNVDSYG HSFGYDLRDK RLQKLIGEVD GFFLDLIEGL QKRNLLKISN
310 320 330 340 350
VMIVSDHGMS NVNANDGEHV VVWERVFPAD AMSAFISHLY NEGPMMMVCL
360 370 380 390 400
KNPRDKQWIC DLIEAQLEKA YGDEISRKFH VILKEDFDPS WKYFQYDNRK
410 420 430 440 450
HRYDDRVGDI WILADEYYAI VKEMGDVPIG IMGTHGYNFN NCSDMASIFI
460 470 480 490
GMGPMFNNEV VPPFENIEVY NMLIKASALL GEEKTKKEKS LLQ
Length:493
Mass (Da):57,355
Last modified:February 1, 1995 - v1
Checksum:i10E67A05C6DEDF09
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti125 – 1251T → I in AAT92706. (PubMed:17322287)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18530 Genomic DNA. Translation: AAB64493.1.
AY692687 Genomic DNA. Translation: AAT92706.1.
BK006939 Genomic DNA. Translation: DAA07638.1.
PIRiS50443.
RefSeqiNP_010900.1. NM_001178831.1.

Genome annotation databases

EnsemblFungiiYEL016C; YEL016C; YEL016C.
GeneIDi856699.
KEGGisce:YEL016C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18530 Genomic DNA. Translation: AAB64493.1 .
AY692687 Genomic DNA. Translation: AAT92706.1 .
BK006939 Genomic DNA. Translation: DAA07638.1 .
PIRi S50443.
RefSeqi NP_010900.1. NM_001178831.1.

3D structure databases

ProteinModelPortali P39997.
SMRi P39997. Positions 79-457.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36715. 10 interactions.
DIPi DIP-5279N.
IntActi P39997. 1 interaction.
MINTi MINT-511815.
STRINGi 4932.YEL016C.

Proteomic databases

PaxDbi P39997.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YEL016C ; YEL016C ; YEL016C .
GeneIDi 856699.
KEGGi sce:YEL016C.

Organism-specific databases

CYGDi YEL016c.
SGDi S000000742. NPP2.

Phylogenomic databases

eggNOGi COG1524.
GeneTreei ENSGT00760000119157.
InParanoidi P39997.
OMAi YDAFRTE.
OrthoDBi EOG74J9HK.

Enzyme and pathway databases

BioCyci YEAST:G3O-30141-MONOMER.
Reactomei REACT_188975. Glycosphingolipid metabolism.

Miscellaneous databases

NextBioi 982760.

Gene expression databases

Genevestigatori P39997.

Family and domain databases

Gene3Di 3.40.720.10. 1 hit.
InterProi IPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024873. E-NPP.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view ]
PANTHERi PTHR10151. PTHR10151. 1 hit.
Pfami PF01663. Phosphodiest. 1 hit.
[Graphical view ]
SUPFAMi SSF53649. SSF53649. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Pho5p and newly identified nucleotide pyrophosphatases/ phosphodiesterases regulate extracellular nucleotide phosphate metabolism in Saccharomyces cerevisiae."
    Kennedy E.J., Pillus L., Ghosh G.
    Eukaryot. Cell 4:1892-1901(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, NPP ACTIVITY, INDUCTION, AUTOPHOSPHORYLATION.

Entry informationi

Entry nameiNPP2_YEAST
AccessioniPrimary (citable) accession number: P39997
Secondary accession number(s): D3DLN4, Q6B2P3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3