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P39997

- NPP2_YEAST

UniProt

P39997 - NPP2_YEAST

Protein

Ectonucleotide pyrophosphatase/phosphodiesterase 2

Gene

NPP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Mediates extracellular nucleotide derived phosphate hydrolysis along with NPP1 and PHO5.1 Publication

    Catalytic activityi

    Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides.
    A dinucleotide + H2O = 2 mononucleotides.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei127 – 1271NucleophileBy similarity

    GO - Molecular functioni

    1. NADH pyrophosphatase activity Source: UniProtKB-EC
    2. nucleoside-triphosphatase activity Source: SGD
    3. nucleoside-triphosphate diphosphatase activity Source: SGD
    4. nucleotide diphosphatase activity Source: SGD
    5. phosphodiesterase I activity Source: SGD

    GO - Biological processi

    1. nucleic acid phosphodiester bond hydrolysis Source: GOC
    2. nucleoside triphosphate metabolic process Source: SGD

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30141-MONOMER.
    ReactomeiREACT_188975. Glycosphingolipid metabolism.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ectonucleotide pyrophosphatase/phosphodiesterase 2
    Short name:
    E-NPP 2
    Including the following 2 domains:
    Alkaline phosphodiesterase 1 (EC:3.1.4.1)
    Nucleotide pyrophosphatase (EC:3.6.1.9)
    Short name:
    NPPase
    Gene namesi
    Name:NPP2
    Ordered Locus Names:YEL016C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYEL016c.
    SGDiS000000742. NPP2.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 493493Ectonucleotide pyrophosphatase/phosphodiesterase 2PRO_0000202612Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi62 – 621N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi69 – 691N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi112 – 1121N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi153 – 1531N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi441 – 4411N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Autophosphorylated as part of the catalytic cycle of phosphodiesterase/pyrophosphatase activity.

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiP39997.

    Expressioni

    Inductioni

    Up-regulated during phosphate starvation.1 Publication

    Gene expression databases

    GenevestigatoriP39997.

    Interactioni

    Protein-protein interaction databases

    BioGridi36715. 10 interactions.
    DIPiDIP-5279N.
    IntActiP39997. 1 interaction.
    MINTiMINT-511815.
    STRINGi4932.YEL016C.

    Structurei

    3D structure databases

    ProteinModelPortaliP39997.
    SMRiP39997. Positions 79-457.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2828CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini46 – 493448ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei29 – 4517Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni76 – 438363PhosphodiesteraseAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1524.
    GeneTreeiENSGT00660000095322.
    OMAiYDAFRTE.
    OrthoDBiEOG74J9HK.

    Family and domain databases

    Gene3Di3.40.720.10. 1 hit.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR017850. Alkaline_phosphatase_core.
    IPR024873. E-NPP.
    IPR002591. Phosphodiest/P_Trfase.
    [Graphical view]
    PANTHERiPTHR10151. PTHR10151. 1 hit.
    PfamiPF01663. Phosphodiest. 1 hit.
    [Graphical view]
    SUPFAMiSSF53649. SSF53649. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P39997-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLLFEQPVDL EKNNEDDTNI KPFAISRHFL LKLLLCGIIL IELLLYSKCP    50
    KPIDNGPRTI ANRSNTYFNG THDFKTLTIL ISIDGFHPRL IDAKYTPFLY 100
    NLHNLRSPYD MNITTAPYMI PSFPTQTFPN HWSMVTGKYP IEHGIVSNIF 150
    WDNFTSSEFR PNNLDARIWS NTADPIWQLL QTESQGEYKV ATHMWPGSEV 200
    VYEDHGDVPR ERMPFYFGKF NQWEKLQDKL AQIFRYIDMP QLKDRPELVI 250
    SYIPNVDSYG HSFGYDLRDK RLQKLIGEVD GFFLDLIEGL QKRNLLKISN 300
    VMIVSDHGMS NVNANDGEHV VVWERVFPAD AMSAFISHLY NEGPMMMVCL 350
    KNPRDKQWIC DLIEAQLEKA YGDEISRKFH VILKEDFDPS WKYFQYDNRK 400
    HRYDDRVGDI WILADEYYAI VKEMGDVPIG IMGTHGYNFN NCSDMASIFI 450
    GMGPMFNNEV VPPFENIEVY NMLIKASALL GEEKTKKEKS LLQ 493
    Length:493
    Mass (Da):57,355
    Last modified:February 1, 1995 - v1
    Checksum:i10E67A05C6DEDF09
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti125 – 1251T → I in AAT92706. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18530 Genomic DNA. Translation: AAB64493.1.
    AY692687 Genomic DNA. Translation: AAT92706.1.
    BK006939 Genomic DNA. Translation: DAA07638.1.
    PIRiS50443.
    RefSeqiNP_010900.1. NM_001178831.1.

    Genome annotation databases

    EnsemblFungiiYEL016C; YEL016C; YEL016C.
    GeneIDi856699.
    KEGGisce:YEL016C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18530 Genomic DNA. Translation: AAB64493.1 .
    AY692687 Genomic DNA. Translation: AAT92706.1 .
    BK006939 Genomic DNA. Translation: DAA07638.1 .
    PIRi S50443.
    RefSeqi NP_010900.1. NM_001178831.1.

    3D structure databases

    ProteinModelPortali P39997.
    SMRi P39997. Positions 79-457.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36715. 10 interactions.
    DIPi DIP-5279N.
    IntActi P39997. 1 interaction.
    MINTi MINT-511815.
    STRINGi 4932.YEL016C.

    Proteomic databases

    PaxDbi P39997.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YEL016C ; YEL016C ; YEL016C .
    GeneIDi 856699.
    KEGGi sce:YEL016C.

    Organism-specific databases

    CYGDi YEL016c.
    SGDi S000000742. NPP2.

    Phylogenomic databases

    eggNOGi COG1524.
    GeneTreei ENSGT00660000095322.
    OMAi YDAFRTE.
    OrthoDBi EOG74J9HK.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30141-MONOMER.
    Reactomei REACT_188975. Glycosphingolipid metabolism.

    Miscellaneous databases

    NextBioi 982760.

    Gene expression databases

    Genevestigatori P39997.

    Family and domain databases

    Gene3Di 3.40.720.10. 1 hit.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR017850. Alkaline_phosphatase_core.
    IPR024873. E-NPP.
    IPR002591. Phosphodiest/P_Trfase.
    [Graphical view ]
    PANTHERi PTHR10151. PTHR10151. 1 hit.
    Pfami PF01663. Phosphodiest. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53649. SSF53649. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Pho5p and newly identified nucleotide pyrophosphatases/ phosphodiesterases regulate extracellular nucleotide phosphate metabolism in Saccharomyces cerevisiae."
      Kennedy E.J., Pillus L., Ghosh G.
      Eukaryot. Cell 4:1892-1901(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, NPP ACTIVITY, INDUCTION, AUTOPHOSPHORYLATION.

    Entry informationi

    Entry nameiNPP2_YEAST
    AccessioniPrimary (citable) accession number: P39997
    Secondary accession number(s): D3DLN4, Q6B2P3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3