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Protein

Ectonucleotide pyrophosphatase/phosphodiesterase 2

Gene

NPP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates extracellular nucleotide derived phosphate hydrolysis along with NPP1 and PHO5.1 Publication

Catalytic activityi

Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides.Curated
A nucleoside triphosphate + H2O = a nucleotide + diphosphate.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei127NucleophileBy similarity1

GO - Molecular functioni

  • NADH pyrophosphatase activity Source: UniProtKB-EC
  • nucleoside-triphosphatase activity Source: SGD
  • nucleoside-triphosphate diphosphatase activity Source: SGD
  • nucleotide diphosphatase activity Source: SGD
  • phosphodiesterase I activity Source: SGD

GO - Biological processi

  • nucleoside triphosphate metabolic process Source: SGD

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme

Enzyme and pathway databases

BioCyciYEAST:G3O-30141-MONOMER
ReactomeiR-SCE-1660662 Glycosphingolipid metabolism
R-SCE-196843 Vitamin B2 (riboflavin) metabolism
R-SCE-6798695 Neutrophil degranulation
R-SCE-6814848 Glycerophospholipid catabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Ectonucleotide pyrophosphatase/phosphodiesterase 2
Short name:
E-NPP 2
Including the following 2 domains:
Alkaline phosphodiesterase 1 (EC:3.1.4.1Curated)
Nucleotide pyrophosphatase (EC:3.6.1.9Curated)
Short name:
NPPase
Alternative name(s):
Nucleotide diphosphataseCurated
Gene namesi
Name:NPP2
Ordered Locus Names:YEL016C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL016C
SGDiS000000742 NPP2

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 28CytoplasmicSequence analysisAdd BLAST28
Transmembranei29 – 45Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST17
Topological domaini46 – 493ExtracellularSequence analysisAdd BLAST448

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002026121 – 493Ectonucleotide pyrophosphatase/phosphodiesterase 2Add BLAST493

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi62N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi69N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi112N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi153N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi441N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Autophosphorylated as part of the catalytic cycle of phosphodiesterase/pyrophosphatase activity.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP39997

Expressioni

Inductioni

Up-regulated during phosphate starvation.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi36715, 48 interactors
DIPiDIP-5279N
IntActiP39997, 3 interactors
MINTiP39997
STRINGi4932.YEL016C

Structurei

3D structure databases

ProteinModelPortaliP39997
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni76 – 438PhosphodiesteraseAdd BLAST363

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00760000119157
InParanoidiP39997
OMAiKVYMYYW
OrthoDBiEOG092C1GZ6

Family and domain databases

Gene3Di3.40.720.10, 2 hits
InterProiView protein in InterPro
IPR017849 Alkaline_Pase-like_a/b/a
IPR017850 Alkaline_phosphatase_core_sf
IPR002591 Phosphodiest/P_Trfase
PfamiView protein in Pfam
PF01663 Phosphodiest, 1 hit
SUPFAMiSSF53649 SSF53649, 1 hit

Sequencei

Sequence statusi: Complete.

P39997-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLFEQPVDL EKNNEDDTNI KPFAISRHFL LKLLLCGIIL IELLLYSKCP
60 70 80 90 100
KPIDNGPRTI ANRSNTYFNG THDFKTLTIL ISIDGFHPRL IDAKYTPFLY
110 120 130 140 150
NLHNLRSPYD MNITTAPYMI PSFPTQTFPN HWSMVTGKYP IEHGIVSNIF
160 170 180 190 200
WDNFTSSEFR PNNLDARIWS NTADPIWQLL QTESQGEYKV ATHMWPGSEV
210 220 230 240 250
VYEDHGDVPR ERMPFYFGKF NQWEKLQDKL AQIFRYIDMP QLKDRPELVI
260 270 280 290 300
SYIPNVDSYG HSFGYDLRDK RLQKLIGEVD GFFLDLIEGL QKRNLLKISN
310 320 330 340 350
VMIVSDHGMS NVNANDGEHV VVWERVFPAD AMSAFISHLY NEGPMMMVCL
360 370 380 390 400
KNPRDKQWIC DLIEAQLEKA YGDEISRKFH VILKEDFDPS WKYFQYDNRK
410 420 430 440 450
HRYDDRVGDI WILADEYYAI VKEMGDVPIG IMGTHGYNFN NCSDMASIFI
460 470 480 490
GMGPMFNNEV VPPFENIEVY NMLIKASALL GEEKTKKEKS LLQ
Length:493
Mass (Da):57,355
Last modified:February 1, 1995 - v1
Checksum:i10E67A05C6DEDF09
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti125T → I in AAT92706 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18530 Genomic DNA Translation: AAB64493.1
AY692687 Genomic DNA Translation: AAT92706.1
BK006939 Genomic DNA Translation: DAA07638.1
PIRiS50443
RefSeqiNP_010900.1, NM_001178831.1

Genome annotation databases

EnsemblFungiiYEL016C; YEL016C; YEL016C
GeneIDi856699
KEGGisce:YEL016C

Similar proteinsi

Entry informationi

Entry nameiNPP2_YEAST
AccessioniPrimary (citable) accession number: P39997
Secondary accession number(s): D3DLN4, Q6B2P3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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