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Protein

Ectonucleotide pyrophosphatase/phosphodiesterase 2

Gene

NPP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates extracellular nucleotide derived phosphate hydrolysis along with NPP1 and PHO5.1 Publication

Catalytic activityi

Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides.
A dinucleotide + H2O = 2 mononucleotides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei127 – 1271NucleophileBy similarity

GO - Molecular functioni

  • NADH pyrophosphatase activity Source: UniProtKB-EC
  • nucleoside-triphosphatase activity Source: SGD
  • nucleoside-triphosphate diphosphatase activity Source: SGD
  • nucleotide diphosphatase activity Source: SGD
  • phosphodiesterase I activity Source: SGD

GO - Biological processi

  • nucleic acid phosphodiester bond hydrolysis Source: GOC
  • nucleoside triphosphate metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciYEAST:G3O-30141-MONOMER.
ReactomeiREACT_307521. Glycosphingolipid metabolism.
REACT_324957. Vitamin B2 (riboflavin) metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Ectonucleotide pyrophosphatase/phosphodiesterase 2
Short name:
E-NPP 2
Including the following 2 domains:
Alkaline phosphodiesterase 1 (EC:3.1.4.1)
Nucleotide pyrophosphatase (EC:3.6.1.9)
Short name:
NPPase
Gene namesi
Name:NPP2
Ordered Locus Names:YEL016C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome V

Organism-specific databases

CYGDiYEL016c.
EuPathDBiFungiDB:YEL016C.
SGDiS000000742. NPP2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2828CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei29 – 4517Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini46 – 493448ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 493493Ectonucleotide pyrophosphatase/phosphodiesterase 2PRO_0000202612Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi62 – 621N-linked (GlcNAc...)Sequence Analysis
Glycosylationi69 – 691N-linked (GlcNAc...)Sequence Analysis
Glycosylationi112 – 1121N-linked (GlcNAc...)Sequence Analysis
Glycosylationi153 – 1531N-linked (GlcNAc...)Sequence Analysis
Glycosylationi441 – 4411N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Autophosphorylated as part of the catalytic cycle of phosphodiesterase/pyrophosphatase activity.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP39997.

Expressioni

Inductioni

Up-regulated during phosphate starvation.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi36715. 10 interactions.
DIPiDIP-5279N.
IntActiP39997. 1 interaction.
MINTiMINT-511815.
STRINGi4932.YEL016C.

Structurei

3D structure databases

ProteinModelPortaliP39997.
SMRiP39997. Positions 79-457.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni76 – 438363PhosphodiesteraseAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1524.
GeneTreeiENSGT00760000119157.
InParanoidiP39997.
OMAiWILADEY.
OrthoDBiEOG74J9HK.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024873. E-NPP.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
PANTHERiPTHR10151. PTHR10151. 1 hit.
PfamiPF01663. Phosphodiest. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.

Sequencei

Sequence statusi: Complete.

P39997-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLFEQPVDL EKNNEDDTNI KPFAISRHFL LKLLLCGIIL IELLLYSKCP
60 70 80 90 100
KPIDNGPRTI ANRSNTYFNG THDFKTLTIL ISIDGFHPRL IDAKYTPFLY
110 120 130 140 150
NLHNLRSPYD MNITTAPYMI PSFPTQTFPN HWSMVTGKYP IEHGIVSNIF
160 170 180 190 200
WDNFTSSEFR PNNLDARIWS NTADPIWQLL QTESQGEYKV ATHMWPGSEV
210 220 230 240 250
VYEDHGDVPR ERMPFYFGKF NQWEKLQDKL AQIFRYIDMP QLKDRPELVI
260 270 280 290 300
SYIPNVDSYG HSFGYDLRDK RLQKLIGEVD GFFLDLIEGL QKRNLLKISN
310 320 330 340 350
VMIVSDHGMS NVNANDGEHV VVWERVFPAD AMSAFISHLY NEGPMMMVCL
360 370 380 390 400
KNPRDKQWIC DLIEAQLEKA YGDEISRKFH VILKEDFDPS WKYFQYDNRK
410 420 430 440 450
HRYDDRVGDI WILADEYYAI VKEMGDVPIG IMGTHGYNFN NCSDMASIFI
460 470 480 490
GMGPMFNNEV VPPFENIEVY NMLIKASALL GEEKTKKEKS LLQ
Length:493
Mass (Da):57,355
Last modified:February 1, 1995 - v1
Checksum:i10E67A05C6DEDF09
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti125 – 1251T → I in AAT92706 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18530 Genomic DNA. Translation: AAB64493.1.
AY692687 Genomic DNA. Translation: AAT92706.1.
BK006939 Genomic DNA. Translation: DAA07638.1.
PIRiS50443.
RefSeqiNP_010900.1. NM_001178831.1.

Genome annotation databases

EnsemblFungiiYEL016C; YEL016C; YEL016C.
GeneIDi856699.
KEGGisce:YEL016C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18530 Genomic DNA. Translation: AAB64493.1.
AY692687 Genomic DNA. Translation: AAT92706.1.
BK006939 Genomic DNA. Translation: DAA07638.1.
PIRiS50443.
RefSeqiNP_010900.1. NM_001178831.1.

3D structure databases

ProteinModelPortaliP39997.
SMRiP39997. Positions 79-457.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36715. 10 interactions.
DIPiDIP-5279N.
IntActiP39997. 1 interaction.
MINTiMINT-511815.
STRINGi4932.YEL016C.

Proteomic databases

PaxDbiP39997.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYEL016C; YEL016C; YEL016C.
GeneIDi856699.
KEGGisce:YEL016C.

Organism-specific databases

CYGDiYEL016c.
EuPathDBiFungiDB:YEL016C.
SGDiS000000742. NPP2.

Phylogenomic databases

eggNOGiCOG1524.
GeneTreeiENSGT00760000119157.
InParanoidiP39997.
OMAiWILADEY.
OrthoDBiEOG74J9HK.

Enzyme and pathway databases

BioCyciYEAST:G3O-30141-MONOMER.
ReactomeiREACT_307521. Glycosphingolipid metabolism.
REACT_324957. Vitamin B2 (riboflavin) metabolism.

Miscellaneous databases

NextBioi982760.
PROiP39997.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024873. E-NPP.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
PANTHERiPTHR10151. PTHR10151. 1 hit.
PfamiPF01663. Phosphodiest. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Pho5p and newly identified nucleotide pyrophosphatases/ phosphodiesterases regulate extracellular nucleotide phosphate metabolism in Saccharomyces cerevisiae."
    Kennedy E.J., Pillus L., Ghosh G.
    Eukaryot. Cell 4:1892-1901(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, NPP ACTIVITY, INDUCTION, AUTOPHOSPHORYLATION.

Entry informationi

Entry nameiNPP2_YEAST
AccessioniPrimary (citable) accession number: P39997
Secondary accession number(s): D3DLN4, Q6B2P3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 24, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.