P39995 (EAF5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chromatin modification-related protein EAF5

Alternative name(s):
ESA1-associated factor 5

Gene names

Name:	EAF5
Ordered Locus Names:	YEL018W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair.
Subunit structure	Component of the NuA4 histone acetyltransferase complex composed of at least ACT1, ARP4, YAF9, VID21, SWC4, EAF3, EAF5, EAF6, EAF7, EPL1, ESA1, TRA1 and YNG2.
Subcellular location	Nucleus Ref.3.
Miscellaneous	Present with 937 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the EAF5 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Chromatin regulator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from direct assay. Source: SGD chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	NuA4 histone acetyltransferase complex Inferred from physical interaction Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	Chromatin modification-related protein EAF5		PRO_0000086892

Sequences

	Sequence		Length	Mass (Da)
	P39995 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 679658AB395EE16A	FASTA	279	31,644
	10 20 30 40 50 60 MDKEVSELVV LQLIHTLISN KNEELVRNGG GINMIGNNLR ISLVKLTNEI QNNLLINELT 70 80 90 100 110 120 NLRRQSNVAN GNRKLGINDI LTIVKNLFPE YRTTLNDGQL SLHGLEMHDI EKLLDEKYDR 130 140 150 160 170 180 FKKTQVEQIR MMEDEILKNG IKTGASQLQP HANAGKSGSA GTSATITTTT PHMAHSMDPK 190 200 210 220 230 240 REKLLKLYRD TVLNKLESKT GNFQKLFKSP DGSIIKNEIN YEDIKNETPG SVHELQLILQ 250 260 270 KSITDGVMRK VIGTDDWKLA RQVQFELDDT VQFMRRALE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"The Yaf9 component of the SWR1 and NuA4 complexes is required for proper gene expression, histone H4 acetylation, and Htz1 replacement near telomeres." Zhang H., Richardson D.O., Roberts D.N., Utley R.T., Erdjument-Bromage H., Tempst P., Cote J., Cairns B.R. Mol. Cell. Biol. 24:9424-9436(2004) [PubMed: 15485911] [Abstract] Cited for: IDENTIFICATION IN THE NUA4 COMPLEX, MASS SPECTROMETRY.
[6]	"Regulation of chromosome stability by the histone H2A variant Htz1, the Swr1 chromatin remodeling complex, and the histone acetyltransferase NuA4." Krogan N.J., Baetz K., Keogh M.-C., Datta N., Sawa C., Kwok T.C.Y., Thompson N.J., Davey M.G., Pootoolal J., Hughes T.R., Emili A., Buratowski S., Hieter P., Greenblatt J.F. Proc. Natl. Acad. Sci. U.S.A. 101:13513-13518(2004) [PubMed: 15353583] [Abstract] Cited for: IDENTIFICATION IN THE NUA4 COMPLEX, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18530 Genomic DNA. Translation: AAB64495.1. BK006939 Genomic DNA. Translation: DAA07635.1.
PIR	S50441.
RefSeq	NP_010897.1. NM_001178833.1.
3D structure databases
ProteinModelPortal	P39995.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5280N.
IntAct	P39995. 21 interactions.
MINT	MINT-485023.
STRING	P39995.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YEL018W; YEL018W; YEL018W.
GeneID	856696.
KEGG	sce:YEL018W.
NMPDR	fig|4932.3.peg.1953.
Organism-specific databases
CYGD	YEL018w.
SGD	S000000744. EAF5.
Phylogenomic databases
eggNOG	fuNOG12724.
OMA	IRRERIM.
OrthoDB	EOG4897X7.
Gene expression databases
ArrayExpress	P39995.
Genevestigator	P39995.
GermOnline	YEL018W. Saccharomyces cerevisiae.
Family and domain databases
KO	K11398.
ProtoNet	Search...
Other
NextBio	982751.

Entry information

Entry name

EAF5_YEAST

Accession

Primary (citable) accession number: P39995
Secondary accession number(s): D3DLN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program