Reviewed, UniProtKB/Swiss-Prot P39994 (YEC0_YEAST)

Names and origin

Protein names

Recommended name:
    Putative 2-hydroxyacyl-CoA lyase
    EC=4.1.-.-

Gene names

Ordered Locus Names:

YEL020C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde By similarity.
Cofactor	Binds 1 magnesium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the TPP enzyme family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Thiamine pyrophosphate
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Ref.2 Inferred from direct assay. Source: SGD
Molecular function	carboxy-lyase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro thiamin pyrophosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 560	560	Putative 2-hydroxyacyl-CoA lyase		PRO_0000090826

Sequences

	Sequence		Length	Mass (Da)
	P39994-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 48523C4E6BCB441F	FASTA	560	61,288
	10 20 30 40 50 60 MTTTATQHFA QLLQKYGIDT VFGIVGIPIV QLADTMVANG IKFIPCRNEQ AASYAASAYG 70 80 90 100 110 120 YISDKPGVLL IVGGPGLIHA LAGIYNSMSN RWPLLVIAGS SSQSDIHKGG FQELDQVSLL 130 140 150 160 170 180 SPFLKFTGKL TPDNIDMITQ KALNYCIQGT AGVSYIDVPA DFIEYEKPLE GNDRTGNELP 190 200 210 220 230 240 MILTPNICGP DPSKIKKVVQ LILQHKNKNI LIVIGKGAVK NSHEIRRLVN TFNLPFLPTP 250 260 270 280 290 300 MAKGIVPDSS PLNVSSARSQ ALKIADIVLV LGARLNWILH FGTSPKWNSE SIFIQFDSNP 310 320 330 340 350 360 ETLGDNNVSP GADLSIWGDI GLSVTALVEE LTRQDSCWKY SGVKQEIREK IQLNQTRLLR 370 380 390 400 410 420 KEKTRGAQLN YNQVYGTLRP LIDDYRTILV TEGANTMDIA RISFPTDAPR RRLDAGTNAT 430 440 450 460 470 480 MGIGLGYALA CKASHPELDV VLIQGDSAFG FSAMEIETAV RCQLALVIVV MNNSGIYHGE 490 500 510 520 530 540 KDIEGDLPPT ALSKNCRYDL VGKGLGANDF FVNTISELSR CFQQAVQLSR TKRETSVINV 550 560 IIEPGEQKQI AFAWQNKPRL

References

[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U18530 Genomic DNA. Translation: AAB64497.1.
PIR	S50439.
RefSeq	NP_010895.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5281N.
IntAct	P39994. 1 interaction.
STRING	P39994.
Proteomic databases
PeptideAtlas	P39994.
Genome annotation databases
Ensembl	YEL020C; YEL020C; YEL020C; Saccharomyces cerevisiae. [Genome view]
GeneID	856694.
KEGG	sce:YEL020C.
NMPDR	fig|4932.3.peg.1950.
Organism-specific databases
CYGD	YEL020c.
SGD	S000000746. YEL020C.
Phylogenomic databases
HOGENOM	P39994.
OMA	IDICAEE
OrthoDB	EOG97PZPG
Gene expression databases
ArrayExpress	P39994.
Genevestigator	P39994.
GermOnline	YEL020C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000399. TPP_bd_CS. IPR012001. TPP_bd_enzyme_N. IPR011766. TPP_enzyme_bd_C. IPR012000. TPP_enzyme_M. [Graphical view]
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	982745.

Entry information

Entry name

YEC0_YEAST

Accession

Primary (citable) accession number: P39994

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 24, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)