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P39993

- GEA2_YEAST

UniProt

P39993 - GEA2_YEAST

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Protein

ARF guanine-nucleotide exchange factor 2

Gene

GEA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Activates the ARF proteins by exchanging bound GDP for free GTP.

GO - Molecular functioni

  1. ARF guanyl-nucleotide exchange factor activity Source: SGD

GO - Biological processi

  1. actin cytoskeleton organization Source: SGD
  2. ER to Golgi vesicle-mediated transport Source: SGD
  3. intra-Golgi vesicle-mediated transport Source: SGD
  4. macroautophagy Source: SGD
  5. positive regulation of GTPase activity Source: GOC
  6. regulation of ARF protein signal transduction Source: InterPro
  7. retrograde vesicle-mediated transport, Golgi to ER Source: SGD
  8. secretory granule organization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

BioCyciYEAST:G3O-30146-MONOMER.
ReactomeiREACT_33101. COPI Mediated Transport.
REACT_94264. Clathrin derived vesicle budding.

Names & Taxonomyi

Protein namesi
Recommended name:
ARF guanine-nucleotide exchange factor 2
Gene namesi
Name:GEA2
Ordered Locus Names:YEL022W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYEL022w.
SGDiS000000748. GEA2.

Subcellular locationi

Cytoplasmcytosol 1 Publication. Membrane 1 Publication; Peripheral membrane protein 1 Publication
Note: Soluble and partially membrane-bound.

GO - Cellular componenti

  1. extrinsic component of membrane Source: SGD
  2. Golgi cis cisterna Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14591459ARF guanine-nucleotide exchange factor 2PRO_0000120213Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei46 – 461Phosphoserine1 Publication
Modified residuei284 – 2841Phosphoserine3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39993.
PaxDbiP39993.
PeptideAtlasiP39993.

Expressioni

Gene expression databases

GenevestigatoriP39993.

Interactioni

Subunit structurei

Interacts with GMH1.2 Publications

Protein-protein interaction databases

BioGridi36707. 51 interactions.
DIPiDIP-6816N.
IntActiP39993. 16 interactions.
MINTiMINT-642174.
STRINGi4932.YEL022W.

Structurei

Secondary structure

1
1459
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi558 – 57215
Helixi574 – 58310
Beta strandi586 – 5883
Helixi592 – 60110
Turni602 – 6054
Helixi608 – 6158
Helixi618 – 6203
Helixi621 – 6299
Helixi638 – 6458
Turni646 – 6483
Helixi655 – 67117
Helixi677 – 6793
Helixi688 – 6903
Helixi695 – 71218
Helixi723 – 7297
Turni730 – 7323
Helixi741 – 75313

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KU1X-ray1.93A/B558-766[»]
ProteinModelPortaliP39993.
SMRiP39993. Positions 558-759.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP39993.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini570 – 714145SEC7PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 SEC7 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5307.
GeneTreeiENSGT00620000087723.
HOGENOMiHOG000248822.
InParanoidiP39993.
KOiK18443.
OMAiGMETIEN.
OrthoDBiEOG725DRW.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
InterProiIPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view]
PfamiPF01369. Sec7. 1 hit.
[Graphical view]
SMARTiSM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48425. SSF48425. 1 hit.
PROSITEiPS50190. SEC7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39993-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDREFVTVD PVTIIIKECI NLSTAMRKYS KFTSQSGVAA LLGGGSEIFS
60 70 80 90 100
NQDDYLAHTF NNLNTNKHND PFLSGFIQLR LMLNKLKNLD NIDSLTILQP
110 120 130 140 150
FLLIVSTSSI SGYITSLALD SLQKFFTLNI INESSQNYIG AHRATVNALT
160 170 180 190 200
HCRFEGSQQL SDDSVLLKVV FLLRSIVDSP YGDLLSNSII YDVLQTILSL
210 220 230 240 250
ACNNRRSEVL RNAAQSTMIA VTVKIFSKLK TIEPVNVNQI YINDESYTND
260 270 280 290 300
VLKADTIGTN VESKEEGSQE DPIGMKVNNE EAISEDDGIE EEHIHSEKST
310 320 330 340 350
NGAEQLDIVQ KTTRSNSRIQ AYADDNYGLP VVRQYLNLLL SLIAPENELK
360 370 380 390 400
HSYSTRIFGL ELIQTALEIS GDRLQLYPRL FTLISDPIFK SILFIIQNTT
410 420 430 440 450
KLSLLQATLQ LFTTLVVILG NNLQLQIELT LTRIFSILLD DGTANNSSSE
460 470 480 490 500
NKNKPSIIKE LLIEQISILW TRSPSFFTST FINFDCNLDR ADVSINFLKA
510 520 530 540 550
LTKLALPESA LTTTESVPPI CLEGLVSLVD DMFDHMKDID REEFGRQKNE
560 570 580 590 600
MEILKKRDRK TEFIECTNAF NEKPKKGIPM LIEKGFIASD SDKDIAEFLF
610 620 630 640 650
NNNNRMNKKT IGLLLCHPDK VSLLNEYIRL FDFSGLRVDE AIRILLTKFR
660 670 680 690 700
LPGESQQIER IIEAFSSAYC ENQDYDPSKI SDNAEDDIST VQPDADSVFI
710 720 730 740 750
LSYSIIMLNT DLHNPQVKEH MSFEDYSGNL KGCCNHKDFP FWYLDRIYCS
760 770 780 790 800
IRDKEIVMPE EHHGNEKWFE DAWNNLISST TVITEIKKDT QSVMDKLTPL
810 820 830 840 850
ELLNFDRAIF KQVGPSIVST LFNIYVVASD DHISTRMITS LDKCSYISAF
860 870 880 890 900
FDFKDLFNDI LNSIAKGTTL INSSHDDELS TLAFEYGPMP LVQIKFEDTN
910 920 930 940 950
TEIPVSTDAV RFGRSFKGQL NTVVFFRIIR RNKDPKIFSK ELWLNIVNII
960 970 980 990 1000
LTLYEDLILS PDIFPDLQKR LKLSNLPKPS PEISINKSKE SKGLLSTFAS
1010 1020 1030 1040 1050
YLKGDEEPTE EEIKSSKKAM ECIKSSNIAA SVFGNESNIT ADLIKTLLDS
1060 1070 1080 1090 1100
AKTEKNADNS RYFEAELLFI IELTIALFLF CKEEKELGKF ILQKVFQLSH
1110 1120 1130 1140 1150
TKGLTKRTVR RMLTYKILLI SLCADQTEYL SKLINDELLK KGDIFTQKFF
1160 1170 1180 1190 1200
ATNQGKEFLK RLFSLTESEF YRGFLLGNEN FWKFLRKVTA MKEQSESIFE
1210 1220 1230 1240 1250
YLNESIKTDS NILTNENFMW VLGLLDEISS MGAVGNHWEI EYKKLTESGH
1260 1270 1280 1290 1300
KIDKENPYKK SIELSLKSIQ LTSHLLEDNN DLRKNEIFAI IQALAHQCIN
1310 1320 1330 1340 1350
PCKQISEFAV VTLEQTLINK IEIPTNEMES VEELIEGGLL PLLNSSETQE
1360 1370 1380 1390 1400
DQKILISSIL TIISNVYLHY LKLGKTSNET FLKILSIFNK FVEDSDIEKK
1410 1420 1430 1440 1450
LQQLILDKKS IEKGNGSSSH GSAHEQTPES NDVEIEATAP IDDNTDDDNK

PKLSDVEKD
Length:1,459
Mass (Da):165,668
Last modified:February 1, 1995 - v1
Checksum:i99DB9FDD34F863FA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18530 Genomic DNA. Translation: AAB64499.1.
BK006939 Genomic DNA. Translation: DAA07630.1.
PIRiS50437.
RefSeqiNP_010892.1. NM_001178837.1.

Genome annotation databases

EnsemblFungiiYEL022W; YEL022W; YEL022W.
GeneIDi856691.
KEGGisce:YEL022W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18530 Genomic DNA. Translation: AAB64499.1 .
BK006939 Genomic DNA. Translation: DAA07630.1 .
PIRi S50437.
RefSeqi NP_010892.1. NM_001178837.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1KU1 X-ray 1.93 A/B 558-766 [» ]
ProteinModelPortali P39993.
SMRi P39993. Positions 558-759.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36707. 51 interactions.
DIPi DIP-6816N.
IntActi P39993. 16 interactions.
MINTi MINT-642174.
STRINGi 4932.YEL022W.

Proteomic databases

MaxQBi P39993.
PaxDbi P39993.
PeptideAtlasi P39993.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YEL022W ; YEL022W ; YEL022W .
GeneIDi 856691.
KEGGi sce:YEL022W.

Organism-specific databases

CYGDi YEL022w.
SGDi S000000748. GEA2.

Phylogenomic databases

eggNOGi COG5307.
GeneTreei ENSGT00620000087723.
HOGENOMi HOG000248822.
InParanoidi P39993.
KOi K18443.
OMAi GMETIEN.
OrthoDBi EOG725DRW.

Enzyme and pathway databases

BioCyci YEAST:G3O-30146-MONOMER.
Reactomei REACT_33101. COPI Mediated Transport.
REACT_94264. Clathrin derived vesicle budding.

Miscellaneous databases

EvolutionaryTracei P39993.
NextBioi 982736.

Gene expression databases

Genevestigatori P39993.

Family and domain databases

Gene3Di 1.10.1000.11. 1 hit.
InterProi IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view ]
Pfami PF01369. Sec7. 1 hit.
[Graphical view ]
SMARTi SM00222. Sec7. 1 hit.
[Graphical view ]
SUPFAMi SSF48425. SSF48425. 1 hit.
PROSITEi PS50190. SEC7. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Nucleotide exchange on ARF mediated by yeast Gea1 protein."
    Peyroche A., Paris S., Jackson C.L.
    Nature 384:479-481(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. "A novel Golgi membrane protein is a partner of the ARF exchange factors Gea1p and Gea2p."
    Chantalat S., Courbeyrette R., Senic-Matuglia F., Jackson C.L., Goud B., Peyroche A.
    Mol. Biol. Cell 14:2357-2371(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GMH1, SUBCELLULAR LOCATION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-284, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-46 AND SER-284, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-284, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Mechanism of domain closure of Sec7 domains and role in BFA sensitivity."
    Renault L., Christova P., Guibert B., Pasqualato S., Cherfils J.
    Biochemistry 41:3605-3612(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.93 ANGSTROMS) OF 558-766 IN COMPLEX WITH GDP AND SEC7.

Entry informationi

Entry nameiGEA2_YEAST
AccessioniPrimary (citable) accession number: P39993
Secondary accession number(s): D3DLM6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3