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P39993

- GEA2_YEAST

UniProt

P39993 - GEA2_YEAST

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Protein
ARF guanine-nucleotide exchange factor 2
Gene
GEA2, YEL022W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Activates the ARF proteins by exchanging bound GDP for free GTP.

GO - Molecular functioni

  1. ARF guanyl-nucleotide exchange factor activity Source: SGD
Complete GO annotation...

GO - Biological processi

  1. ER to Golgi vesicle-mediated transport Source: SGD
  2. actin cytoskeleton organization Source: SGD
  3. intra-Golgi vesicle-mediated transport Source: SGD
  4. macroautophagy Source: SGD
  5. regulation of ARF protein signal transduction Source: InterPro
  6. regulation of GTPase activity Source: GOC
  7. retrograde vesicle-mediated transport, Golgi to ER Source: SGD
  8. secretory granule organization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

BioCyciYEAST:G3O-30146-MONOMER.
ReactomeiREACT_33101. COPI Mediated Transport.
REACT_94264. Clathrin derived vesicle budding.

Names & Taxonomyi

Protein namesi
Recommended name:
ARF guanine-nucleotide exchange factor 2
Gene namesi
Name:GEA2
Ordered Locus Names:YEL022W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYEL022w.
SGDiS000000748. GEA2.

Subcellular locationi

Cytoplasmcytosol. Membrane; Peripheral membrane protein
Note: Soluble and partially membrane-bound.1 Publication

GO - Cellular componenti

  1. Golgi cis cisterna Source: SGD
  2. cytosol Source: UniProtKB-SubCell
  3. extrinsic component of membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14591459ARF guanine-nucleotide exchange factor 2
PRO_0000120213Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei46 – 461Phosphoserine1 Publication
Modified residuei284 – 2841Phosphoserine3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39993.
PaxDbiP39993.
PeptideAtlasiP39993.

Expressioni

Gene expression databases

GenevestigatoriP39993.

Interactioni

Subunit structurei

Interacts with GMH1.1 Publication

Protein-protein interaction databases

BioGridi36707. 51 interactions.
DIPiDIP-6816N.
IntActiP39993. 16 interactions.
MINTiMINT-642174.
STRINGi4932.YEL022W.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi558 – 57215
Helixi574 – 58310
Beta strandi586 – 5883
Helixi592 – 60110
Turni602 – 6054
Helixi608 – 6158
Helixi618 – 6203
Helixi621 – 6299
Helixi638 – 6458
Turni646 – 6483
Helixi655 – 67117
Helixi677 – 6793
Helixi688 – 6903
Helixi695 – 71218
Helixi723 – 7297
Turni730 – 7323
Helixi741 – 75313

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KU1X-ray1.93A/B558-766[»]
ProteinModelPortaliP39993.
SMRiP39993. Positions 558-759.

Miscellaneous databases

EvolutionaryTraceiP39993.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini570 – 714145SEC7
Add
BLAST

Sequence similaritiesi

Contains 1 SEC7 domain.

Phylogenomic databases

eggNOGiCOG5307.
GeneTreeiENSGT00620000087723.
HOGENOMiHOG000248822.
OMAiGMETIEN.
OrthoDBiEOG725DRW.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
InterProiIPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view]
PfamiPF01369. Sec7. 1 hit.
[Graphical view]
SMARTiSM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48425. SSF48425. 1 hit.
PROSITEiPS50190. SEC7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39993-1 [UniParc]FASTAAdd to Basket

« Hide

MSDREFVTVD PVTIIIKECI NLSTAMRKYS KFTSQSGVAA LLGGGSEIFS     50
NQDDYLAHTF NNLNTNKHND PFLSGFIQLR LMLNKLKNLD NIDSLTILQP 100
FLLIVSTSSI SGYITSLALD SLQKFFTLNI INESSQNYIG AHRATVNALT 150
HCRFEGSQQL SDDSVLLKVV FLLRSIVDSP YGDLLSNSII YDVLQTILSL 200
ACNNRRSEVL RNAAQSTMIA VTVKIFSKLK TIEPVNVNQI YINDESYTND 250
VLKADTIGTN VESKEEGSQE DPIGMKVNNE EAISEDDGIE EEHIHSEKST 300
NGAEQLDIVQ KTTRSNSRIQ AYADDNYGLP VVRQYLNLLL SLIAPENELK 350
HSYSTRIFGL ELIQTALEIS GDRLQLYPRL FTLISDPIFK SILFIIQNTT 400
KLSLLQATLQ LFTTLVVILG NNLQLQIELT LTRIFSILLD DGTANNSSSE 450
NKNKPSIIKE LLIEQISILW TRSPSFFTST FINFDCNLDR ADVSINFLKA 500
LTKLALPESA LTTTESVPPI CLEGLVSLVD DMFDHMKDID REEFGRQKNE 550
MEILKKRDRK TEFIECTNAF NEKPKKGIPM LIEKGFIASD SDKDIAEFLF 600
NNNNRMNKKT IGLLLCHPDK VSLLNEYIRL FDFSGLRVDE AIRILLTKFR 650
LPGESQQIER IIEAFSSAYC ENQDYDPSKI SDNAEDDIST VQPDADSVFI 700
LSYSIIMLNT DLHNPQVKEH MSFEDYSGNL KGCCNHKDFP FWYLDRIYCS 750
IRDKEIVMPE EHHGNEKWFE DAWNNLISST TVITEIKKDT QSVMDKLTPL 800
ELLNFDRAIF KQVGPSIVST LFNIYVVASD DHISTRMITS LDKCSYISAF 850
FDFKDLFNDI LNSIAKGTTL INSSHDDELS TLAFEYGPMP LVQIKFEDTN 900
TEIPVSTDAV RFGRSFKGQL NTVVFFRIIR RNKDPKIFSK ELWLNIVNII 950
LTLYEDLILS PDIFPDLQKR LKLSNLPKPS PEISINKSKE SKGLLSTFAS 1000
YLKGDEEPTE EEIKSSKKAM ECIKSSNIAA SVFGNESNIT ADLIKTLLDS 1050
AKTEKNADNS RYFEAELLFI IELTIALFLF CKEEKELGKF ILQKVFQLSH 1100
TKGLTKRTVR RMLTYKILLI SLCADQTEYL SKLINDELLK KGDIFTQKFF 1150
ATNQGKEFLK RLFSLTESEF YRGFLLGNEN FWKFLRKVTA MKEQSESIFE 1200
YLNESIKTDS NILTNENFMW VLGLLDEISS MGAVGNHWEI EYKKLTESGH 1250
KIDKENPYKK SIELSLKSIQ LTSHLLEDNN DLRKNEIFAI IQALAHQCIN 1300
PCKQISEFAV VTLEQTLINK IEIPTNEMES VEELIEGGLL PLLNSSETQE 1350
DQKILISSIL TIISNVYLHY LKLGKTSNET FLKILSIFNK FVEDSDIEKK 1400
LQQLILDKKS IEKGNGSSSH GSAHEQTPES NDVEIEATAP IDDNTDDDNK 1450
PKLSDVEKD 1459
Length:1,459
Mass (Da):165,668
Last modified:February 1, 1995 - v1
Checksum:i99DB9FDD34F863FA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18530 Genomic DNA. Translation: AAB64499.1.
BK006939 Genomic DNA. Translation: DAA07630.1.
PIRiS50437.
RefSeqiNP_010892.1. NM_001178837.1.

Genome annotation databases

EnsemblFungiiYEL022W; YEL022W; YEL022W.
GeneIDi856691.
KEGGisce:YEL022W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18530 Genomic DNA. Translation: AAB64499.1 .
BK006939 Genomic DNA. Translation: DAA07630.1 .
PIRi S50437.
RefSeqi NP_010892.1. NM_001178837.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1KU1 X-ray 1.93 A/B 558-766 [» ]
ProteinModelPortali P39993.
SMRi P39993. Positions 558-759.
ModBasei Search...

Protein-protein interaction databases

BioGridi 36707. 51 interactions.
DIPi DIP-6816N.
IntActi P39993. 16 interactions.
MINTi MINT-642174.
STRINGi 4932.YEL022W.

Proteomic databases

MaxQBi P39993.
PaxDbi P39993.
PeptideAtlasi P39993.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YEL022W ; YEL022W ; YEL022W .
GeneIDi 856691.
KEGGi sce:YEL022W.

Organism-specific databases

CYGDi YEL022w.
SGDi S000000748. GEA2.

Phylogenomic databases

eggNOGi COG5307.
GeneTreei ENSGT00620000087723.
HOGENOMi HOG000248822.
OMAi GMETIEN.
OrthoDBi EOG725DRW.

Enzyme and pathway databases

BioCyci YEAST:G3O-30146-MONOMER.
Reactomei REACT_33101. COPI Mediated Transport.
REACT_94264. Clathrin derived vesicle budding.

Miscellaneous databases

EvolutionaryTracei P39993.
NextBioi 982736.

Gene expression databases

Genevestigatori P39993.

Family and domain databases

Gene3Di 1.10.1000.11. 1 hit.
InterProi IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view ]
Pfami PF01369. Sec7. 1 hit.
[Graphical view ]
SMARTi SM00222. Sec7. 1 hit.
[Graphical view ]
SUPFAMi SSF48425. SSF48425. 1 hit.
PROSITEi PS50190. SEC7. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Nucleotide exchange on ARF mediated by yeast Gea1 protein."
    Peyroche A., Paris S., Jackson C.L.
    Nature 384:479-481(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. "A novel Golgi membrane protein is a partner of the ARF exchange factors Gea1p and Gea2p."
    Chantalat S., Courbeyrette R., Senic-Matuglia F., Jackson C.L., Goud B., Peyroche A.
    Mol. Biol. Cell 14:2357-2371(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GMH1, SUBCELLULAR LOCATION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-284, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-46 AND SER-284, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-284, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Mechanism of domain closure of Sec7 domains and role in BFA sensitivity."
    Renault L., Christova P., Guibert B., Pasqualato S., Cherfils J.
    Biochemistry 41:3605-3612(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.93 ANGSTROMS) OF 558-766 IN COMPLEX WITH GDP AND SEC7.

Entry informationi

Entry nameiGEA2_YEAST
AccessioniPrimary (citable) accession number: P39993
Secondary accession number(s): D3DLM6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 3, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7700 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

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