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P39993

- GEA2_YEAST

UniProt

P39993 - GEA2_YEAST

Protein

ARF guanine-nucleotide exchange factor 2

Gene

GEA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Activates the ARF proteins by exchanging bound GDP for free GTP.

    GO - Molecular functioni

    1. ARF guanyl-nucleotide exchange factor activity Source: SGD

    GO - Biological processi

    1. actin cytoskeleton organization Source: SGD
    2. ER to Golgi vesicle-mediated transport Source: SGD
    3. intra-Golgi vesicle-mediated transport Source: SGD
    4. macroautophagy Source: SGD
    5. positive regulation of GTPase activity Source: GOC
    6. regulation of ARF protein signal transduction Source: InterPro
    7. retrograde vesicle-mediated transport, Golgi to ER Source: SGD
    8. secretory granule organization Source: SGD

    Keywords - Molecular functioni

    Guanine-nucleotide releasing factor

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30146-MONOMER.
    ReactomeiREACT_33101. COPI Mediated Transport.
    REACT_94264. Clathrin derived vesicle budding.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ARF guanine-nucleotide exchange factor 2
    Gene namesi
    Name:GEA2
    Ordered Locus Names:YEL022W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYEL022w.
    SGDiS000000748. GEA2.

    Subcellular locationi

    Cytoplasmcytosol 1 Publication. Membrane 1 Publication; Peripheral membrane protein 1 Publication
    Note: Soluble and partially membrane-bound.

    GO - Cellular componenti

    1. cytosol Source: UniProtKB-SubCell
    2. extrinsic component of membrane Source: SGD
    3. Golgi cis cisterna Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 14591459ARF guanine-nucleotide exchange factor 2PRO_0000120213Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei46 – 461Phosphoserine1 Publication
    Modified residuei284 – 2841Phosphoserine3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP39993.
    PaxDbiP39993.
    PeptideAtlasiP39993.

    Expressioni

    Gene expression databases

    GenevestigatoriP39993.

    Interactioni

    Subunit structurei

    Interacts with GMH1.2 Publications

    Protein-protein interaction databases

    BioGridi36707. 51 interactions.
    DIPiDIP-6816N.
    IntActiP39993. 16 interactions.
    MINTiMINT-642174.
    STRINGi4932.YEL022W.

    Structurei

    Secondary structure

    1
    1459
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi558 – 57215
    Helixi574 – 58310
    Beta strandi586 – 5883
    Helixi592 – 60110
    Turni602 – 6054
    Helixi608 – 6158
    Helixi618 – 6203
    Helixi621 – 6299
    Helixi638 – 6458
    Turni646 – 6483
    Helixi655 – 67117
    Helixi677 – 6793
    Helixi688 – 6903
    Helixi695 – 71218
    Helixi723 – 7297
    Turni730 – 7323
    Helixi741 – 75313

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1KU1X-ray1.93A/B558-766[»]
    ProteinModelPortaliP39993.
    SMRiP39993. Positions 558-759.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP39993.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini570 – 714145SEC7PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 SEC7 domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5307.
    GeneTreeiENSGT00620000087723.
    HOGENOMiHOG000248822.
    OMAiGMETIEN.
    OrthoDBiEOG725DRW.

    Family and domain databases

    Gene3Di1.10.1000.11. 1 hit.
    InterProiIPR023394. Sec7_alpha_orthog.
    IPR000904. Sec7_dom.
    [Graphical view]
    PfamiPF01369. Sec7. 1 hit.
    [Graphical view]
    SMARTiSM00222. Sec7. 1 hit.
    [Graphical view]
    SUPFAMiSSF48425. SSF48425. 1 hit.
    PROSITEiPS50190. SEC7. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39993-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDREFVTVD PVTIIIKECI NLSTAMRKYS KFTSQSGVAA LLGGGSEIFS     50
    NQDDYLAHTF NNLNTNKHND PFLSGFIQLR LMLNKLKNLD NIDSLTILQP 100
    FLLIVSTSSI SGYITSLALD SLQKFFTLNI INESSQNYIG AHRATVNALT 150
    HCRFEGSQQL SDDSVLLKVV FLLRSIVDSP YGDLLSNSII YDVLQTILSL 200
    ACNNRRSEVL RNAAQSTMIA VTVKIFSKLK TIEPVNVNQI YINDESYTND 250
    VLKADTIGTN VESKEEGSQE DPIGMKVNNE EAISEDDGIE EEHIHSEKST 300
    NGAEQLDIVQ KTTRSNSRIQ AYADDNYGLP VVRQYLNLLL SLIAPENELK 350
    HSYSTRIFGL ELIQTALEIS GDRLQLYPRL FTLISDPIFK SILFIIQNTT 400
    KLSLLQATLQ LFTTLVVILG NNLQLQIELT LTRIFSILLD DGTANNSSSE 450
    NKNKPSIIKE LLIEQISILW TRSPSFFTST FINFDCNLDR ADVSINFLKA 500
    LTKLALPESA LTTTESVPPI CLEGLVSLVD DMFDHMKDID REEFGRQKNE 550
    MEILKKRDRK TEFIECTNAF NEKPKKGIPM LIEKGFIASD SDKDIAEFLF 600
    NNNNRMNKKT IGLLLCHPDK VSLLNEYIRL FDFSGLRVDE AIRILLTKFR 650
    LPGESQQIER IIEAFSSAYC ENQDYDPSKI SDNAEDDIST VQPDADSVFI 700
    LSYSIIMLNT DLHNPQVKEH MSFEDYSGNL KGCCNHKDFP FWYLDRIYCS 750
    IRDKEIVMPE EHHGNEKWFE DAWNNLISST TVITEIKKDT QSVMDKLTPL 800
    ELLNFDRAIF KQVGPSIVST LFNIYVVASD DHISTRMITS LDKCSYISAF 850
    FDFKDLFNDI LNSIAKGTTL INSSHDDELS TLAFEYGPMP LVQIKFEDTN 900
    TEIPVSTDAV RFGRSFKGQL NTVVFFRIIR RNKDPKIFSK ELWLNIVNII 950
    LTLYEDLILS PDIFPDLQKR LKLSNLPKPS PEISINKSKE SKGLLSTFAS 1000
    YLKGDEEPTE EEIKSSKKAM ECIKSSNIAA SVFGNESNIT ADLIKTLLDS 1050
    AKTEKNADNS RYFEAELLFI IELTIALFLF CKEEKELGKF ILQKVFQLSH 1100
    TKGLTKRTVR RMLTYKILLI SLCADQTEYL SKLINDELLK KGDIFTQKFF 1150
    ATNQGKEFLK RLFSLTESEF YRGFLLGNEN FWKFLRKVTA MKEQSESIFE 1200
    YLNESIKTDS NILTNENFMW VLGLLDEISS MGAVGNHWEI EYKKLTESGH 1250
    KIDKENPYKK SIELSLKSIQ LTSHLLEDNN DLRKNEIFAI IQALAHQCIN 1300
    PCKQISEFAV VTLEQTLINK IEIPTNEMES VEELIEGGLL PLLNSSETQE 1350
    DQKILISSIL TIISNVYLHY LKLGKTSNET FLKILSIFNK FVEDSDIEKK 1400
    LQQLILDKKS IEKGNGSSSH GSAHEQTPES NDVEIEATAP IDDNTDDDNK 1450
    PKLSDVEKD 1459
    Length:1,459
    Mass (Da):165,668
    Last modified:February 1, 1995 - v1
    Checksum:i99DB9FDD34F863FA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18530 Genomic DNA. Translation: AAB64499.1.
    BK006939 Genomic DNA. Translation: DAA07630.1.
    PIRiS50437.
    RefSeqiNP_010892.1. NM_001178837.1.

    Genome annotation databases

    EnsemblFungiiYEL022W; YEL022W; YEL022W.
    GeneIDi856691.
    KEGGisce:YEL022W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18530 Genomic DNA. Translation: AAB64499.1 .
    BK006939 Genomic DNA. Translation: DAA07630.1 .
    PIRi S50437.
    RefSeqi NP_010892.1. NM_001178837.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1KU1 X-ray 1.93 A/B 558-766 [» ]
    ProteinModelPortali P39993.
    SMRi P39993. Positions 558-759.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36707. 51 interactions.
    DIPi DIP-6816N.
    IntActi P39993. 16 interactions.
    MINTi MINT-642174.
    STRINGi 4932.YEL022W.

    Proteomic databases

    MaxQBi P39993.
    PaxDbi P39993.
    PeptideAtlasi P39993.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YEL022W ; YEL022W ; YEL022W .
    GeneIDi 856691.
    KEGGi sce:YEL022W.

    Organism-specific databases

    CYGDi YEL022w.
    SGDi S000000748. GEA2.

    Phylogenomic databases

    eggNOGi COG5307.
    GeneTreei ENSGT00620000087723.
    HOGENOMi HOG000248822.
    OMAi GMETIEN.
    OrthoDBi EOG725DRW.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30146-MONOMER.
    Reactomei REACT_33101. COPI Mediated Transport.
    REACT_94264. Clathrin derived vesicle budding.

    Miscellaneous databases

    EvolutionaryTracei P39993.
    NextBioi 982736.

    Gene expression databases

    Genevestigatori P39993.

    Family and domain databases

    Gene3Di 1.10.1000.11. 1 hit.
    InterProi IPR023394. Sec7_alpha_orthog.
    IPR000904. Sec7_dom.
    [Graphical view ]
    Pfami PF01369. Sec7. 1 hit.
    [Graphical view ]
    SMARTi SM00222. Sec7. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48425. SSF48425. 1 hit.
    PROSITEi PS50190. SEC7. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Nucleotide exchange on ARF mediated by yeast Gea1 protein."
      Peyroche A., Paris S., Jackson C.L.
      Nature 384:479-481(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    4. "A novel Golgi membrane protein is a partner of the ARF exchange factors Gea1p and Gea2p."
      Chantalat S., Courbeyrette R., Senic-Matuglia F., Jackson C.L., Goud B., Peyroche A.
      Mol. Biol. Cell 14:2357-2371(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GMH1, SUBCELLULAR LOCATION.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-284, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-46 AND SER-284, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-284, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Mechanism of domain closure of Sec7 domains and role in BFA sensitivity."
      Renault L., Christova P., Guibert B., Pasqualato S., Cherfils J.
      Biochemistry 41:3605-3612(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.93 ANGSTROMS) OF 558-766 IN COMPLEX WITH GDP AND SEC7.

    Entry informationi

    Entry nameiGEA2_YEAST
    AccessioniPrimary (citable) accession number: P39993
    Secondary accession number(s): D3DLM6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 126 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 7700 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3