ARF guanine-nucleotide exchange factor 2 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P39993 (GEA2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 113. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
ARF guanine-nucleotide exchange factor 2

Gene names

Name:	GEA2
Ordered Locus Names:	YEL022W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	1459 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Activates the ARF proteins by exchanging bound GDP for free GTP.
Subunit structure	Interacts with GMH1. Ref.4
Subcellular location	Cytoplasm › cytosol. Membrane; Peripheral membrane protein. Note: Soluble and partially membrane-bound. Ref.4
Miscellaneous	Present with 7700 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 SEC7 domain.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Molecular function	Guanine-nucleotide releasing factor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER to Golgi vesicle-mediated transport Inferred from mutant phenotype PubMed 11493664. Source: SGD actin cytoskeleton organization Inferred from genetic interaction PubMed 14668359. Source: SGD intra-Golgi vesicle-mediated transport Inferred from mutant phenotype PubMed 11493664. Source: SGD macroautophagy Inferred from mutant phenotype PubMed 20444982. Source: SGD regulation of ARF protein signal transduction Inferred from electronic annotation. Source: InterPro retrograde vesicle-mediated transport, Golgi to ER Inferred from genetic interaction PubMed 11294905. Source: SGD secretory granule organization Inferred from mutant phenotype PubMed 14734650. Source: SGD
Cellular_component	Golgi cis cisterna Inferred from direct assay PubMed 11294905. Source: SGD cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to membrane Inferred from direct assay PubMed 15930122. Source: SGD integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD
Molecular_function	ARF guanyl-nucleotide exchange factor activity Inferred from direct assay PubMed 10430582. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1459

1459

ARF guanine-nucleotide exchange factor 2

PRO_0000120213

Regions

Domain

570 – 714

145

SEC7

Amino acid modifications

Modified residue

Phosphoserine Ref.9

Modified residue

284

Phosphoserine Ref.6 Ref.7 Ref.8 Ref.9

Modified residue

313

Phosphothreonine Ref.8 Ref.9

Modified residue

317

Phosphoserine Ref.6 Ref.9

Secondary structure

1459

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P39993 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 99DB9FDD34F863FA
Show »

FASTA

1,459

165,668

        10         20         30         40         50         60 
MSDREFVTVD PVTIIIKECI NLSTAMRKYS KFTSQSGVAA LLGGGSEIFS NQDDYLAHTF 

        70         80         90        100        110        120 
NNLNTNKHND PFLSGFIQLR LMLNKLKNLD NIDSLTILQP FLLIVSTSSI SGYITSLALD 

       130        140        150        160        170        180 
SLQKFFTLNI INESSQNYIG AHRATVNALT HCRFEGSQQL SDDSVLLKVV FLLRSIVDSP 

       190        200        210        220        230        240 
YGDLLSNSII YDVLQTILSL ACNNRRSEVL RNAAQSTMIA VTVKIFSKLK TIEPVNVNQI 

       250        260        270        280        290        300 
YINDESYTND VLKADTIGTN VESKEEGSQE DPIGMKVNNE EAISEDDGIE EEHIHSEKST 

       310        320        330        340        350        360 
NGAEQLDIVQ KTTRSNSRIQ AYADDNYGLP VVRQYLNLLL SLIAPENELK HSYSTRIFGL 

       370        380        390        400        410        420 
ELIQTALEIS GDRLQLYPRL FTLISDPIFK SILFIIQNTT KLSLLQATLQ LFTTLVVILG 

       430        440        450        460        470        480 
NNLQLQIELT LTRIFSILLD DGTANNSSSE NKNKPSIIKE LLIEQISILW TRSPSFFTST 

       490        500        510        520        530        540 
FINFDCNLDR ADVSINFLKA LTKLALPESA LTTTESVPPI CLEGLVSLVD DMFDHMKDID 

       550        560        570        580        590        600 
REEFGRQKNE MEILKKRDRK TEFIECTNAF NEKPKKGIPM LIEKGFIASD SDKDIAEFLF 

       610        620        630        640        650        660 
NNNNRMNKKT IGLLLCHPDK VSLLNEYIRL FDFSGLRVDE AIRILLTKFR LPGESQQIER 

       670        680        690        700        710        720 
IIEAFSSAYC ENQDYDPSKI SDNAEDDIST VQPDADSVFI LSYSIIMLNT DLHNPQVKEH 

       730        740        750        760        770        780 
MSFEDYSGNL KGCCNHKDFP FWYLDRIYCS IRDKEIVMPE EHHGNEKWFE DAWNNLISST 

       790        800        810        820        830        840 
TVITEIKKDT QSVMDKLTPL ELLNFDRAIF KQVGPSIVST LFNIYVVASD DHISTRMITS 

       850        860        870        880        890        900 
LDKCSYISAF FDFKDLFNDI LNSIAKGTTL INSSHDDELS TLAFEYGPMP LVQIKFEDTN 

       910        920        930        940        950        960 
TEIPVSTDAV RFGRSFKGQL NTVVFFRIIR RNKDPKIFSK ELWLNIVNII LTLYEDLILS 

       970        980        990       1000       1010       1020 
PDIFPDLQKR LKLSNLPKPS PEISINKSKE SKGLLSTFAS YLKGDEEPTE EEIKSSKKAM 

      1030       1040       1050       1060       1070       1080 
ECIKSSNIAA SVFGNESNIT ADLIKTLLDS AKTEKNADNS RYFEAELLFI IELTIALFLF 

      1090       1100       1110       1120       1130       1140 
CKEEKELGKF ILQKVFQLSH TKGLTKRTVR RMLTYKILLI SLCADQTEYL SKLINDELLK 

      1150       1160       1170       1180       1190       1200 
KGDIFTQKFF ATNQGKEFLK RLFSLTESEF YRGFLLGNEN FWKFLRKVTA MKEQSESIFE 

      1210       1220       1230       1240       1250       1260 
YLNESIKTDS NILTNENFMW VLGLLDEISS MGAVGNHWEI EYKKLTESGH KIDKENPYKK 

      1270       1280       1290       1300       1310       1320 
SIELSLKSIQ LTSHLLEDNN DLRKNEIFAI IQALAHQCIN PCKQISEFAV VTLEQTLINK 

      1330       1340       1350       1360       1370       1380 
IEIPTNEMES VEELIEGGLL PLLNSSETQE DQKILISSIL TIISNVYLHY LKLGKTSNET 

      1390       1400       1410       1420       1430       1440 
FLKILSIFNK FVEDSDIEKK LQQLILDKKS IEKGNGSSSH GSAHEQTPES NDVEIEATAP 

      1450 
IDDNTDDDNK PKLSDVEKD

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Nucleotide exchange on ARF mediated by yeast Gea1 protein." Peyroche A., Paris S., Jackson C.L. Nature 384:479-481(1996) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[4]	"A novel Golgi membrane protein is a partner of the ARF exchange factors Gea1p and Gea2p." Chantalat S., Courbeyrette R., Senic-Matuglia F., Jackson C.L., Goud B., Peyroche A. Mol. Biol. Cell 14:2357-2371(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GMH1, SUBCELLULAR LOCATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-284 AND SER-317, MASS SPECTROMETRY. Strain: YAL6B.
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-284, MASS SPECTROMETRY. Strain: ADR376.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-284 AND THR-313, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-46; SER-284; THR-313 AND SER-317, MASS SPECTROMETRY.
[10]	"Mechanism of domain closure of Sec7 domains and role in BFA sensitivity." Renault L., Christova P., Guibert B., Pasqualato S., Cherfils J. Biochemistry 41:3605-3612(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.93 ANGSTROMS) OF 558-766 IN COMPLEX WITH GDP AND SEC7.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U18530 Genomic DNA. Translation: AAB64499.1.
BK006939 Genomic DNA. Translation: DAA07630.1.

PIR

S50437.

RefSeq

NP_010892.1. NM_001178837.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KU1	X-ray	1.93	A/B	558-766	[»]

ProteinModelPortal

P39993.

SMR

P39993. Positions 558-759.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-6816N.

IntAct

P39993. 16 interactions.

MINT

MINT-642174.

STRING

4932.YEL022W.

Proteomic databases

PaxDb

P39993.

PeptideAtlas

P39993.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YEL022W; YEL022W; YEL022W.

GeneID

856691.

KEGG

sce:YEL022W.

Organism-specific databases

CYGD

YEL022w.

SGD

S000000748. GEA2.

Phylogenomic databases

eggNOG

COG5307.

GeneTree

ENSGT00700000104554.

HOGENOM

HOG000248822.

OMA

ANFFFSK.

OrthoDB

EOG48D43D.

Gene expression databases

Genevestigator

P39993.

GermOnline

YEL022W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

1.10.1000.11. 1 hit.

InterPro

IPR000904. Sec7.
IPR023394. SEC7_alpha_orthog.
[Graphical view]

Pfam

PF01369. Sec7. 1 hit.
[Graphical view]

SMART

SM00222. Sec7. 1 hit.
[Graphical view]

SUPFAM

SSF48425. Sec7. 1 hit.

PROSITE

PS50190. SEC7. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P39993.

NextBio

982736.

Entry information

Entry name

GEA2_YEAST

Accession

Primary (citable) accession number: P39993
Secondary accession number(s): D3DLM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents