ARF guanine-nucleotide exchange factor 2 - Saccharomyces cerevisiae (Baker's yeast)

Sequence

>sp|P39993|GEA2_YEAST ARF guanine-nucleotide exchange factor 2 OS=Saccharomyces cerevisiae GN=GEA2 PE=1 SV=1 MSDREFVTVDPVTIIIKECINLSTAMRKYSKFTSQSGVAALLGGGSEIFSNQDDYLAHTF NNLNTNKHNDPFLSGFIQLRLMLNKLKNLDNIDSLTILQPFLLIVSTSSISGYITSLALD SLQKFFTLNIINESSQNYIGAHRATVNALTHCRFEGSQQLSDDSVLLKVVFLLRSIVDSP YGDLLSNSIIYDVLQTILSLACNNRRSEVLRNAAQSTMIAVTVKIFSKLKTIEPVNVNQI YINDESYTNDVLKADTIGTNVESKEEGSQEDPIGMKVNNEEAISEDDGIEEEHIHSEKST NGAEQLDIVQKTTRSNSRIQAYADDNYGLPVVRQYLNLLLSLIAPENELKHSYSTRIFGL ELIQTALEISGDRLQLYPRLFTLISDPIFKSILFIIQNTTKLSLLQATLQLFTTLVVILG NNLQLQIELTLTRIFSILLDDGTANNSSSENKNKPSIIKELLIEQISILWTRSPSFFTST FINFDCNLDRADVSINFLKALTKLALPESALTTTESVPPICLEGLVSLVDDMFDHMKDID REEFGRQKNEMEILKKRDRKTEFIECTNAFNEKPKKGIPMLIEKGFIASDSDKDIAEFLF NNNNRMNKKTIGLLLCHPDKVSLLNEYIRLFDFSGLRVDEAIRILLTKFRLPGESQQIER IIEAFSSAYCENQDYDPSKISDNAEDDISTVQPDADSVFILSYSIIMLNTDLHNPQVKEH MSFEDYSGNLKGCCNHKDFPFWYLDRIYCSIRDKEIVMPEEHHGNEKWFEDAWNNLISST TVITEIKKDTQSVMDKLTPLELLNFDRAIFKQVGPSIVSTLFNIYVVASDDHISTRMITS LDKCSYISAFFDFKDLFNDILNSIAKGTTLINSSHDDELSTLAFEYGPMPLVQIKFEDTN TEIPVSTDAVRFGRSFKGQLNTVVFFRIIRRNKDPKIFSKELWLNIVNIILTLYEDLILS PDIFPDLQKRLKLSNLPKPSPEISINKSKESKGLLSTFASYLKGDEEPTEEEIKSSKKAM ECIKSSNIAASVFGNESNITADLIKTLLDSAKTEKNADNSRYFEAELLFIIELTIALFLF CKEEKELGKFILQKVFQLSHTKGLTKRTVRRMLTYKILLISLCADQTEYLSKLINDELLK KGDIFTQKFFATNQGKEFLKRLFSLTESEFYRGFLLGNENFWKFLRKVTAMKEQSESIFE YLNESIKTDSNILTNENFMWVLGLLDEISSMGAVGNHWEIEYKKLTESGHKIDKENPYKK SIELSLKSIQLTSHLLEDNNDLRKNEIFAIIQALAHQCINPCKQISEFAVVTLEQTLINK IEIPTNEMESVEELIEGGLLPLLNSSETQEDQKILISSILTIISNVYLHYLKLGKTSNET FLKILSIFNKFVEDSDIEKKLQQLILDKKSIEKGNGSSSHGSAHEQTPESNDVEIEATAP IDDNTDDDNKPKLSDVEKD

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Reviewed, UniProtKB/Swiss-Prot P39993 (GEA2_YEAST)

Last modified November 24, 2009. Version 83. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
ARF guanine-nucleotide exchange factor 2

Gene names

Name:	GEA2
Ordered Locus Names:	YEL022W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	1459 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Activates the ARF proteins by exchanging bound GDP for free GTP.
Subunit structure	Interacts with GMH1. Ref.3
Subcellular location	Cytoplasm › cytosol. Membrane; Peripheral membrane protein. Note: Soluble and partially membrane-bound. Ref.3
Miscellaneous	Present with 7700 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Contains 1 SEC7 domain.

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Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Molecular function	Guanine-nucleotide releasing factor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Inferred from mutant phenotype. Source: SGD actin cytoskeleton organization Inferred from genetic interaction. Source: SGD intra-Golgi vesicle-mediated transport Inferred from mutant phenotype. Source: SGD regulation of ARF protein signal transduction Inferred from electronic annotation. Source: InterPro retrograde vesicle-mediated transport, Golgi to ER Inferred from genetic interaction. Source: SGD secretory granule organization Inferred from mutant phenotype. Source: SGD
Cellular component	Golgi cis cisterna Inferred from direct assay. Source: SGD cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to membrane Inferred from direct assay. Source: SGD
Molecular function	ARF guanyl-nucleotide exchange factor activity Inferred from direct assay. Source: SGD
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1459

1459

ARF guanine-nucleotide exchange factor 2

PRO_0000120213

Regions

Domain

570 – 714

145

SEC7

Amino acid modifications

Modified residue

Phosphoserine Ref.8

Modified residue

284

Phosphoserine Ref.8 Ref.5 Ref.6 Ref.7

Modified residue

313

Phosphothreonine Ref.8 Ref.7

Modified residue

317

Phosphoserine Ref.8 Ref.5

Secondary structure

1459

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P39993-1 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 99DB9FDD34F863FA
Show »

FASTA

1,459

165,668

        10         20         30         40         50         60 
MSDREFVTVD PVTIIIKECI NLSTAMRKYS KFTSQSGVAA LLGGGSEIFS NQDDYLAHTF 

        70         80         90        100        110        120 
NNLNTNKHND PFLSGFIQLR LMLNKLKNLD NIDSLTILQP FLLIVSTSSI SGYITSLALD 

       130        140        150        160        170        180 
SLQKFFTLNI INESSQNYIG AHRATVNALT HCRFEGSQQL SDDSVLLKVV FLLRSIVDSP 

       190        200        210        220        230        240 
YGDLLSNSII YDVLQTILSL ACNNRRSEVL RNAAQSTMIA VTVKIFSKLK TIEPVNVNQI 

       250        260        270        280        290        300 
YINDESYTND VLKADTIGTN VESKEEGSQE DPIGMKVNNE EAISEDDGIE EEHIHSEKST 

       310        320        330        340        350        360 
NGAEQLDIVQ KTTRSNSRIQ AYADDNYGLP VVRQYLNLLL SLIAPENELK HSYSTRIFGL 

       370        380        390        400        410        420 
ELIQTALEIS GDRLQLYPRL FTLISDPIFK SILFIIQNTT KLSLLQATLQ LFTTLVVILG 

       430        440        450        460        470        480 
NNLQLQIELT LTRIFSILLD DGTANNSSSE NKNKPSIIKE LLIEQISILW TRSPSFFTST 

       490        500        510        520        530        540 
FINFDCNLDR ADVSINFLKA LTKLALPESA LTTTESVPPI CLEGLVSLVD DMFDHMKDID 

       550        560        570        580        590        600 
REEFGRQKNE MEILKKRDRK TEFIECTNAF NEKPKKGIPM LIEKGFIASD SDKDIAEFLF 

       610        620        630        640        650        660 
NNNNRMNKKT IGLLLCHPDK VSLLNEYIRL FDFSGLRVDE AIRILLTKFR LPGESQQIER 

       670        680        690        700        710        720 
IIEAFSSAYC ENQDYDPSKI SDNAEDDIST VQPDADSVFI LSYSIIMLNT DLHNPQVKEH 

       730        740        750        760        770        780 
MSFEDYSGNL KGCCNHKDFP FWYLDRIYCS IRDKEIVMPE EHHGNEKWFE DAWNNLISST 

       790        800        810        820        830        840 
TVITEIKKDT QSVMDKLTPL ELLNFDRAIF KQVGPSIVST LFNIYVVASD DHISTRMITS 

       850        860        870        880        890        900 
LDKCSYISAF FDFKDLFNDI LNSIAKGTTL INSSHDDELS TLAFEYGPMP LVQIKFEDTN 

       910        920        930        940        950        960 
TEIPVSTDAV RFGRSFKGQL NTVVFFRIIR RNKDPKIFSK ELWLNIVNII LTLYEDLILS 

       970        980        990       1000       1010       1020 
PDIFPDLQKR LKLSNLPKPS PEISINKSKE SKGLLSTFAS YLKGDEEPTE EEIKSSKKAM 

      1030       1040       1050       1060       1070       1080 
ECIKSSNIAA SVFGNESNIT ADLIKTLLDS AKTEKNADNS RYFEAELLFI IELTIALFLF 

      1090       1100       1110       1120       1130       1140 
CKEEKELGKF ILQKVFQLSH TKGLTKRTVR RMLTYKILLI SLCADQTEYL SKLINDELLK 

      1150       1160       1170       1180       1190       1200 
KGDIFTQKFF ATNQGKEFLK RLFSLTESEF YRGFLLGNEN FWKFLRKVTA MKEQSESIFE 

      1210       1220       1230       1240       1250       1260 
YLNESIKTDS NILTNENFMW VLGLLDEISS MGAVGNHWEI EYKKLTESGH KIDKENPYKK 

      1270       1280       1290       1300       1310       1320 
SIELSLKSIQ LTSHLLEDNN DLRKNEIFAI IQALAHQCIN PCKQISEFAV VTLEQTLINK 

      1330       1340       1350       1360       1370       1380 
IEIPTNEMES VEELIEGGLL PLLNSSETQE DQKILISSIL TIISNVYLHY LKLGKTSNET 

      1390       1400       1410       1420       1430       1440 
FLKILSIFNK FVEDSDIEKK LQQLILDKKS IEKGNGSSSH GSAHEQTPES NDVEIEATAP 

      1450 
IDDNTDDDNK PKLSDVEKD

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Nucleotide exchange on ARF mediated by yeast Gea1 protein." Peyroche A., Paris S., Jackson C.L. Nature 384:479-481(1996) [PubMed: 8945477] [Abstract] Cited for: CHARACTERIZATION.
[3]	"A novel Golgi membrane protein is a partner of the ARF exchange factors Gea1p and Gea2p." Chantalat S., Courbeyrette R., Senic-Matuglia F., Jackson C.L., Goud B., Peyroche A. Mol. Biol. Cell 14:2357-2371(2003) [PubMed: 12808035] [Abstract] Cited for: INTERACTION WITH GMH1, SUBCELLULAR LOCATION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-284 AND SER-317, MASS SPECTROMETRY.
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-284, MASS SPECTROMETRY.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-284 AND THR-313, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-46; SER-284; THR-313 AND SER-317, MASS SPECTROMETRY.
[9]	"Mechanism of domain closure of Sec7 domains and role in BFA sensitivity." Renault L., Christova P., Guibert B., Pasqualato S., Cherfils J. Biochemistry 41:3605-3612(2002) [PubMed: 11888276] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.93 ANGSTROMS) OF 558-766 IN COMPLEX WITH GDP AND SEC7.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

U18530 Genomic DNA. Translation: AAB64499.1.

PIR

S50437.

RefSeq

NP_010892.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KU1	X-ray	1.93	A/B	558-766	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:6816N.

STRING

P39993.

Proteomic databases

PeptideAtlas

P39993.

PRIDE

P39993.

Genome annotation databases

Ensembl

YEL022W; YEL022W; YEL022W; Saccharomyces cerevisiae. [Genome view]

GeneID

856691.

KEGG

sce:YEL022W.

NMPDR

fig|4932.3.peg.1946.

Organism-specific databases

CYGD

YEL022w.

SGD

S000000748. GEA2.

Phylogenomic databases

HOGENOM

P39993.

OMA

SGFIQLR

OrthoDB

EOG9FXSQX

Gene expression databases

ArrayExpress

P39993.

Genevestigator

P39993.

GermOnline

YEL022W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR000904. Sec7.
[Graphical view]

Pfam

PF01369. Sec7. 1 hit.
[Graphical view]

SMART

SM00222. Sec7. 1 hit.
[Graphical view]

PROSITE

PS50190. SEC7. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

982736.

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Entry information

Entry name

GEA2_YEAST

Accession

Primary (citable) accession number: P39993

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 24, 2009
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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