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P39993 (GEA2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ARF guanine-nucleotide exchange factor 2
Gene names
Name:GEA2
Ordered Locus Names:YEL022W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1459 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Activates the ARF proteins by exchanging bound GDP for free GTP.

Subunit structure

Interacts with GMH1. Ref.4

Subcellular location

Cytoplasmcytosol. Membrane; Peripheral membrane protein. Note: Soluble and partially membrane-bound. Ref.4

Miscellaneous

Present with 7700 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 SEC7 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14591459ARF guanine-nucleotide exchange factor 2
PRO_0000120213

Regions

Domain570 – 714145SEC7

Amino acid modifications

Modified residue461Phosphoserine Ref.7
Modified residue2841Phosphoserine Ref.6 Ref.7 Ref.8

Secondary structure

............................... 1459
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P39993 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 99DB9FDD34F863FA

FASTA1,459165,668
        10         20         30         40         50         60 
MSDREFVTVD PVTIIIKECI NLSTAMRKYS KFTSQSGVAA LLGGGSEIFS NQDDYLAHTF 

        70         80         90        100        110        120 
NNLNTNKHND PFLSGFIQLR LMLNKLKNLD NIDSLTILQP FLLIVSTSSI SGYITSLALD 

       130        140        150        160        170        180 
SLQKFFTLNI INESSQNYIG AHRATVNALT HCRFEGSQQL SDDSVLLKVV FLLRSIVDSP 

       190        200        210        220        230        240 
YGDLLSNSII YDVLQTILSL ACNNRRSEVL RNAAQSTMIA VTVKIFSKLK TIEPVNVNQI 

       250        260        270        280        290        300 
YINDESYTND VLKADTIGTN VESKEEGSQE DPIGMKVNNE EAISEDDGIE EEHIHSEKST 

       310        320        330        340        350        360 
NGAEQLDIVQ KTTRSNSRIQ AYADDNYGLP VVRQYLNLLL SLIAPENELK HSYSTRIFGL 

       370        380        390        400        410        420 
ELIQTALEIS GDRLQLYPRL FTLISDPIFK SILFIIQNTT KLSLLQATLQ LFTTLVVILG 

       430        440        450        460        470        480 
NNLQLQIELT LTRIFSILLD DGTANNSSSE NKNKPSIIKE LLIEQISILW TRSPSFFTST 

       490        500        510        520        530        540 
FINFDCNLDR ADVSINFLKA LTKLALPESA LTTTESVPPI CLEGLVSLVD DMFDHMKDID 

       550        560        570        580        590        600 
REEFGRQKNE MEILKKRDRK TEFIECTNAF NEKPKKGIPM LIEKGFIASD SDKDIAEFLF 

       610        620        630        640        650        660 
NNNNRMNKKT IGLLLCHPDK VSLLNEYIRL FDFSGLRVDE AIRILLTKFR LPGESQQIER 

       670        680        690        700        710        720 
IIEAFSSAYC ENQDYDPSKI SDNAEDDIST VQPDADSVFI LSYSIIMLNT DLHNPQVKEH 

       730        740        750        760        770        780 
MSFEDYSGNL KGCCNHKDFP FWYLDRIYCS IRDKEIVMPE EHHGNEKWFE DAWNNLISST 

       790        800        810        820        830        840 
TVITEIKKDT QSVMDKLTPL ELLNFDRAIF KQVGPSIVST LFNIYVVASD DHISTRMITS 

       850        860        870        880        890        900 
LDKCSYISAF FDFKDLFNDI LNSIAKGTTL INSSHDDELS TLAFEYGPMP LVQIKFEDTN 

       910        920        930        940        950        960 
TEIPVSTDAV RFGRSFKGQL NTVVFFRIIR RNKDPKIFSK ELWLNIVNII LTLYEDLILS 

       970        980        990       1000       1010       1020 
PDIFPDLQKR LKLSNLPKPS PEISINKSKE SKGLLSTFAS YLKGDEEPTE EEIKSSKKAM 

      1030       1040       1050       1060       1070       1080 
ECIKSSNIAA SVFGNESNIT ADLIKTLLDS AKTEKNADNS RYFEAELLFI IELTIALFLF 

      1090       1100       1110       1120       1130       1140 
CKEEKELGKF ILQKVFQLSH TKGLTKRTVR RMLTYKILLI SLCADQTEYL SKLINDELLK 

      1150       1160       1170       1180       1190       1200 
KGDIFTQKFF ATNQGKEFLK RLFSLTESEF YRGFLLGNEN FWKFLRKVTA MKEQSESIFE 

      1210       1220       1230       1240       1250       1260 
YLNESIKTDS NILTNENFMW VLGLLDEISS MGAVGNHWEI EYKKLTESGH KIDKENPYKK 

      1270       1280       1290       1300       1310       1320 
SIELSLKSIQ LTSHLLEDNN DLRKNEIFAI IQALAHQCIN PCKQISEFAV VTLEQTLINK 

      1330       1340       1350       1360       1370       1380 
IEIPTNEMES VEELIEGGLL PLLNSSETQE DQKILISSIL TIISNVYLHY LKLGKTSNET 

      1390       1400       1410       1420       1430       1440 
FLKILSIFNK FVEDSDIEKK LQQLILDKKS IEKGNGSSSH GSAHEQTPES NDVEIEATAP 

      1450 
IDDNTDDDNK PKLSDVEKD 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Nucleotide exchange on ARF mediated by yeast Gea1 protein."
Peyroche A., Paris S., Jackson C.L.
Nature 384:479-481(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[4]"A novel Golgi membrane protein is a partner of the ARF exchange factors Gea1p and Gea2p."
Chantalat S., Courbeyrette R., Senic-Matuglia F., Jackson C.L., Goud B., Peyroche A.
Mol. Biol. Cell 14:2357-2371(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GMH1, SUBCELLULAR LOCATION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-284, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-46 AND SER-284, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-284, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Mechanism of domain closure of Sec7 domains and role in BFA sensitivity."
Renault L., Christova P., Guibert B., Pasqualato S., Cherfils J.
Biochemistry 41:3605-3612(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.93 ANGSTROMS) OF 558-766 IN COMPLEX WITH GDP AND SEC7.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U18530 Genomic DNA. Translation: AAB64499.1.
BK006939 Genomic DNA. Translation: DAA07630.1.
PIRS50437.
RefSeqNP_010892.1. NM_001178837.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1KU1X-ray1.93A/B558-766[»]
ProteinModelPortalP39993.
SMRP39993. Positions 558-759.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36707. 51 interactions.
DIPDIP-6816N.
IntActP39993. 16 interactions.
MINTMINT-642174.
STRING4932.YEL022W.

Proteomic databases

MaxQBP39993.
PaxDbP39993.
PeptideAtlasP39993.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYEL022W; YEL022W; YEL022W.
GeneID856691.
KEGGsce:YEL022W.

Organism-specific databases

CYGDYEL022w.
SGDS000000748. GEA2.

Phylogenomic databases

eggNOGCOG5307.
GeneTreeENSGT00620000087723.
HOGENOMHOG000248822.
OMAGMETIEN.
OrthoDBEOG725DRW.

Enzyme and pathway databases

BioCycYEAST:G3O-30146-MONOMER.

Gene expression databases

GenevestigatorP39993.

Family and domain databases

Gene3D1.10.1000.11. 1 hit.
InterProIPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view]
PfamPF01369. Sec7. 1 hit.
[Graphical view]
SMARTSM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMSSF48425. SSF48425. 1 hit.
PROSITEPS50190. SEC7. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP39993.
NextBio982736.

Entry information

Entry nameGEA2_YEAST
AccessionPrimary (citable) accession number: P39993
Secondary accession number(s): D3DLM6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 14, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references