Reviewed,
UniProtKB/Swiss-Prot P39990 (SNU13_YEAST)
Last modified
November 24, 2009.
Version 86.
History...
Clusters with 100%,
90%,
50% identity |
Documents (5) |
Third-party data |
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Names and origin
| Protein names | Recommended name: 13 kDa ribonucleoprotein-associated protein Alternative name(s): Small nuclear ribonucleoprotein-associated protein 1 | ||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||
| Taxonomic identifier | 4932 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 126 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Common component of the spliceosome and rRNA processing machinery. In association with the spliceosomal U4/U6.U5 tri-snRNP particle, required for splicing of pre-mRNA. In association with box C/D snoRNPs, required for processing of pre-ribosomal RNA (rRNA) and site-specific 2'-O-methylation of substrate RNAs. Essential for the accumulation and stability of U4 snRNA, U6 snRNA, and box C/D snoRNAs. Ref.5 Ref.7 Ref.10 |
| Subunit structure | Binds to the C'/D and B/C motifs in U3 snoRNA. Component of the 25S U4/U6.U5 tri-snRNP particle, a subcomplex of the spliceosome. Binds to the 5'-stem-loop of U4 snRNA. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. |
| Subcellular location | |
| Sequence similarities | Belongs to the ribosomal protein L7Ae family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Ribosome biogenesis mRNA processing mRNA splicing rRNA processing |
| Cellular component | Nucleus Spliceosome |
| Ligand | RNA-binding |
| Molecular function | Ribonucleoprotein |
| Technical term | 3D-structure Complete proteome |
| Gene Ontology (GO) | |
| Biological process | maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Ref.10 Inferred from mutant phenotype. Source: SGD nuclear mRNA splicing, via spliceosome Ref.4 Ref.10Inferred from mutant phenotype. Source: SGD |
| Cellular component | U4/U6 x U5 tri-snRNP complex Ref.3 Ref.4 Inferred from direct assay. Source: SGD box C/D snoRNP complex Ref.5Inferred from direct assay. Source: SGD small-subunit processome Ref.8Inferred from direct assay. Source: SGD spliceosomal complexInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | RNA binding Ref.10 Inferred from mutant phenotype. Source: SGD |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||||||||||||||||||||
Molecule processing | ||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 126 | 126 | 13 kDa ribonucleoprotein-associated protein | PRO_0000136777 | ||||||||||||||||||||||||
Experimental info | ||||||||||||||||||||||||||||
| Mutagenesis | 59 | 1 | E → A: Impairs binding to U4 snRNA, but not U3 snoRNA. Causes pre-mRNA splicing and pre-rRNA processing defects. Ref.10 | |||||||||||||||||||||||||
| Mutagenesis | 81 | 1 | V → L: Impairs binding to U4 snRNA, but not U3 snoRNA, and causes pre rRNA processing defects and an accumulation of unspliced U3 snoRNA; when associated with A-84. Ref.10 | |||||||||||||||||||||||||
| Mutagenesis | 84 | 1 | R → A: Impairs binding to U4 snRNA, but not U3 snoRNA, and causes pre rRNA processing defects and an accumulation of unspliced U3 snoRNA; when associated with L-81. Ref.10 | |||||||||||||||||||||||||
Secondary structure | ||||||||||||||||||||||||||||
Helix Strand Turn | ||||||||||||||||||||||||||||
| Helix | 14 – 30 | 17 | ||||||||||||||||||||||||||
| Beta strand | 33 – 36 | 4 | ||||||||||||||||||||||||||
| Helix | 37 – 46 | 10 | ||||||||||||||||||||||||||
| Beta strand | 49 – 55 | 7 | ||||||||||||||||||||||||||
| Helix | 61 – 64 | 4 | ||||||||||||||||||||||||||
| Helix | 66 – 74 | 9 | ||||||||||||||||||||||||||
| Beta strand | 78 – 82 | 5 | ||||||||||||||||||||||||||
| Helix | 84 – 90 | 7 | ||||||||||||||||||||||||||
| Beta strand | 98 – 103 | 6 | ||||||||||||||||||||||||||
| Helix | 111 – 124 | 14 | ||||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W.Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [2] | "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J.Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [3] | "Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP." Gottschalk A., Neubauer G., Banroques J., Mann M., Luehrmann R., Fabrizio P. EMBO J. 18:4535-4548(1999) [PubMed: 10449419] [Abstract] Cited for: IDENTIFICATION IN U4/U6.U5 TRI-SNRNP COMPLEX, MASS SPECTROMETRY. |
| [4] | "Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins." Stevens S.W., Abelson J. Proc. Natl. Acad. Sci. U.S.A. 96:7226-7231(1999) [PubMed: 10377396] [Abstract] Cited for: IDENTIFICATION IN U4/U6.U5 TRI-SNRNP COMPLEX, MASS SPECTROMETRY. |
| [5] | "A common core RNP structure shared between the small nucleoar box C/D RNPs and the spliceosomal U4 snRNP." Watkins N.J., Segault V., Charpentier B., Nottrott S., Fabrizio P., Bachi A., Wilm M., Rosbash M., Branlant C., Luehrmann R. Cell 103:457-466(2000) [PubMed: 11081632] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN U3 SNORNP, MASS SPECTROMETRY, RNA-BINDING. |
| [6] | "Composition and functional characterization of the yeast spliceosomal penta-snRNP." Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D., Abelson J. Mol. Cell 9:31-44(2002) [PubMed: 11804584] [Abstract] Cited for: IDENTIFICATION IN U1.U2.U4/U6.U5 PENTA-SNRNP COMPLEX, MASS SPECTROMETRY. |
| [7] | "Purified box C/D snoRNPs are able to reproduce site-specific 2'-O-methylation of target RNA in vitro." Galardi S., Fatica A., Bachi A., Scaloni A., Presutti C., Bozzoni I. Mol. Cell. Biol. 22:6663-6668(2002) [PubMed: 12215523] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN U3 SNORNP, MASS SPECTROMETRY. |
| [8] | "A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis." Dragon F., Gallagher J.E.G., Compagnone-Post P.A., Mitchell B.M., Porwancher K.A., Wehner K.A., Wormsley S., Settlage R.E., Shabanowitz J., Osheim Y., Beyer A.L., Hunt D.F., Baserga S.J. Nature 417:967-970(2002) [PubMed: 12068309] [Abstract] Cited for: IDENTIFICATION IN SSU PROCESSOME BY MASS SPECTROMETRY. |
| [9] | "A structural, phylogenetic, and functional study of 15.5-kD/Snu13 protein binding on U3 small nucleolar RNA." Marmier-Gourrier N., Clery A., Senty-Segault V., Charpentier B., Schlotter F., Leclerc F., Fournier R., Branlant C. RNA 9:821-838(2003) [PubMed: 12810916] [Abstract] Cited for: RNA-BINDING. |
| [10] | "Analysis of Snu13p mutations reveals differential interactions with the U4 snRNA and U3 snoRNA." Dobbyn H.C., O'Keefe R.T. RNA 10:308-320(2004) [PubMed: 14730029] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF GLU-59; VAL-81 AND ARG-84, RNA-BINDING. |
| [11] | "Structural comparison of yeast snoRNP and spliceosomal protein Snu13p with its homologs." Oruganti S., Zhang Y., Li H. Biochem. Biophys. Res. Commun. 333:550-554(2005) [PubMed: 15963469] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS). |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| U18530 Genomic DNA. Translation: AAB64503.1. AY558377 Genomic DNA. Translation: AAS56703.1. | |||||||||||||||||||
| PIR | S50433. | ||||||||||||||||||
| RefSeq | NP_010888.1. | ||||||||||||||||||
3D structure databases | |||||||||||||||||||
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| ModBase | Search... | ||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||
| DIP | DIP:2870N. | ||||||||||||||||||
| IntAct | P39990. 11 interactions. | ||||||||||||||||||
| STRING | P39990. | ||||||||||||||||||
Proteomic databases | |||||||||||||||||||
| PeptideAtlas | P39990. | ||||||||||||||||||
| PRIDE | P39990. | ||||||||||||||||||
Genome annotation databases | |||||||||||||||||||
| Ensembl | YEL026W; YEL026W; YEL026W; Saccharomyces cerevisiae. [Genome view] | ||||||||||||||||||
| GeneID | 856687. | ||||||||||||||||||
| KEGG | sce:YEL026W. | ||||||||||||||||||
| NMPDR | fig|4932.3.peg.1942. | ||||||||||||||||||
Organism-specific databases | |||||||||||||||||||
| CYGD | YEL026w. | ||||||||||||||||||
| SGD | S000000752. SNU13. | ||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||
| HOGENOM | P39990. | ||||||||||||||||||
| OMA | GVNPKAF | ||||||||||||||||||
| OrthoDB | EOG973R7R | ||||||||||||||||||
Gene expression databases | |||||||||||||||||||
| ArrayExpress | P39990. | ||||||||||||||||||
| Genevestigator | P39990. | ||||||||||||||||||
| GermOnline | YEL026W. Saccharomyces cerevisiae. | ||||||||||||||||||
Family and domain databases | |||||||||||||||||||
| InterPro | IPR002415. HMG_nuclear. IPR018492. Ribosomal_L7A/RS6_fam. IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45. IPR004037. Ribosomal_L7Ae_CS. [Graphical view] | ||||||||||||||||||
| Pfam | PF01248. Ribosomal_L7Ae. 1 hit. [Graphical view] | ||||||||||||||||||
| PRINTS | PR00881. L7ARS6FAMILY. PR00883. NUCLEARHMG. | ||||||||||||||||||
| PROSITE | PS01082. RIBOSOMAL_L7AE. 1 hit. [Graphical view] | ||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||
Other Resources | |||||||||||||||||||
| NextBio | 982724. | ||||||||||||||||||
Entry information
| Entry name | SNU13_YEAST | ||||||||
| Accession | Primary (citable) accession number: P39990 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| Ribosomal proteins Ribosomal proteins families and list of entries |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome V Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names |

Clusters with


