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P39990

- SNU13_YEAST

UniProt

P39990 - SNU13_YEAST

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Protein

13 kDa ribonucleoprotein-associated protein

Gene

SNU13

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Common component of the spliceosome and rRNA processing machinery. In association with the spliceosomal U4/U6.U5 tri-snRNP particle, required for splicing of pre-mRNA. In association with box C/D snoRNPs, required for processing of pre-ribosomal RNA (rRNA) and site-specific 2'-O-methylation of substrate RNAs. Essential for the accumulation and stability of U4 snRNA, U6 snRNA, and box C/D snoRNAs.3 Publications

GO - Molecular functioni

  1. RNA binding Source: SGD

GO - Biological processi

  1. maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  2. mRNA splicing, via spliceosome Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing, Ribosome biogenesis, rRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30149-MONOMER.
ReactomeiREACT_191540. mRNA Splicing - Minor Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
13 kDa ribonucleoprotein-associated protein
Alternative name(s):
Small nuclear ribonucleoprotein-associated protein 1
Gene namesi
Name:SNU13
Ordered Locus Names:YEL026W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYEL026w.
SGDiS000000752. SNU13.

Subcellular locationi

GO - Cellular componenti

  1. box C/D snoRNP complex Source: SGD
  2. nucleolus Source: SGD
  3. small-subunit processome Source: SGD
  4. spliceosomal complex Source: UniProtKB-KW
  5. U4/U6 x U5 tri-snRNP complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi59 – 591E → A: Impairs binding to U4 snRNA, but not U3 snoRNA. Causes pre-mRNA splicing and pre-rRNA processing defects. 1 Publication
Mutagenesisi81 – 811V → L: Impairs binding to U4 snRNA, but not U3 snoRNA, and causes pre rRNA processing defects and an accumulation of unspliced U3 snoRNA; when associated with A-84. 1 Publication
Mutagenesisi84 – 841R → A: Impairs binding to U4 snRNA, but not U3 snoRNA, and causes pre rRNA processing defects and an accumulation of unspliced U3 snoRNA; when associated with L-81. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12612613 kDa ribonucleoprotein-associated proteinPRO_0000136777Add
BLAST

Proteomic databases

MaxQBiP39990.
PaxDbiP39990.
PeptideAtlasiP39990.

Expressioni

Gene expression databases

GenevestigatoriP39990.

Interactioni

Subunit structurei

Binds to the C'/D and B/C motifs in U3 snoRNA. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1. Binds to the 5'-stem-loop of U4 snRNA. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3.6 Publications

Protein-protein interaction databases

BioGridi36703. 56 interactions.
DIPiDIP-2870N.
IntActiP39990. 18 interactions.
MINTiMINT-627070.
STRINGi4932.YEL026W.

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi14 – 2916
Beta strandi33 – 364
Helixi37 – 4610
Beta strandi49 – 557
Helixi61 – 644
Helixi66 – 749
Beta strandi78 – 825
Helixi84 – 907
Beta strandi98 – 1036
Helixi111 – 12616

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZWZX-ray1.90A/B1-126[»]
2ALEX-ray1.80A1-126[»]
ProteinModelPortaliP39990.
SMRiP39990. Positions 1-126.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP39990.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L7Ae family.Curated

Phylogenomic databases

eggNOGiCOG1358.
GeneTreeiENSGT00550000074840.
HOGENOMiHOG000055226.
InParanoidiP39990.
KOiK12845.
OMAiQWIPLEN.
OrthoDBiEOG790GCX.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
InterProiIPR002415. H/ACA_rnp_Nhp2_euk.
IPR029064. L30e-like.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR018492. Ribosomal_L7Ae/L8/Nhp2.
IPR004037. Ribosomal_L7Ae_CS.
[Graphical view]
PfamiPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
PRINTSiPR00881. L7ARS6FAMILY.
PR00883. NUCLEARHMG.
SUPFAMiSSF55315. SSF55315. 1 hit.
PROSITEiPS01082. RIBOSOMAL_L7AE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39990-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSAPNPKAFP LADAALTQQI LDVVQQAANL RQLKKGANEA TKTLNRGISE
60 70 80 90 100
FIIMAADCEP IEILLHLPLL CEDKNVPYVF VPSRVALGRA CGVSRPVIAA
110 120
SITTNDASAI KTQIYAVKDK IETLLI
Length:126
Mass (Da):13,569
Last modified:February 1, 1995 - v1
Checksum:i4489780153E8F182
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18530 Genomic DNA. Translation: AAB64503.1.
AY558377 Genomic DNA. Translation: AAS56703.1.
BK006939 Genomic DNA. Translation: DAA07626.1.
PIRiS50433.
RefSeqiNP_010888.1. NM_001178841.1.

Genome annotation databases

EnsemblFungiiYEL026W; YEL026W; YEL026W.
GeneIDi856687.
KEGGisce:YEL026W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18530 Genomic DNA. Translation: AAB64503.1 .
AY558377 Genomic DNA. Translation: AAS56703.1 .
BK006939 Genomic DNA. Translation: DAA07626.1 .
PIRi S50433.
RefSeqi NP_010888.1. NM_001178841.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1ZWZ X-ray 1.90 A/B 1-126 [» ]
2ALE X-ray 1.80 A 1-126 [» ]
ProteinModelPortali P39990.
SMRi P39990. Positions 1-126.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36703. 56 interactions.
DIPi DIP-2870N.
IntActi P39990. 18 interactions.
MINTi MINT-627070.
STRINGi 4932.YEL026W.

Proteomic databases

MaxQBi P39990.
PaxDbi P39990.
PeptideAtlasi P39990.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YEL026W ; YEL026W ; YEL026W .
GeneIDi 856687.
KEGGi sce:YEL026W.

Organism-specific databases

CYGDi YEL026w.
SGDi S000000752. SNU13.

Phylogenomic databases

eggNOGi COG1358.
GeneTreei ENSGT00550000074840.
HOGENOMi HOG000055226.
InParanoidi P39990.
KOi K12845.
OMAi QWIPLEN.
OrthoDBi EOG790GCX.

Enzyme and pathway databases

BioCyci YEAST:G3O-30149-MONOMER.
Reactomei REACT_191540. mRNA Splicing - Minor Pathway.

Miscellaneous databases

EvolutionaryTracei P39990.
NextBioi 982724.
PROi P39990.

Gene expression databases

Genevestigatori P39990.

Family and domain databases

Gene3Di 3.30.1330.30. 1 hit.
InterProi IPR002415. H/ACA_rnp_Nhp2_euk.
IPR029064. L30e-like.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR018492. Ribosomal_L7Ae/L8/Nhp2.
IPR004037. Ribosomal_L7Ae_CS.
[Graphical view ]
Pfami PF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view ]
PRINTSi PR00881. L7ARS6FAMILY.
PR00883. NUCLEARHMG.
SUPFAMi SSF55315. SSF55315. 1 hit.
PROSITEi PS01082. RIBOSOMAL_L7AE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP."
    Gottschalk A., Neubauer G., Banroques J., Mann M., Luehrmann R., Fabrizio P.
    EMBO J. 18:4535-4548(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  5. "Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins."
    Stevens S.W., Abelson J.
    Proc. Natl. Acad. Sci. U.S.A. 96:7226-7231(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN U4/U6.U5 TRI-SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  6. "A common core RNP structure shared between the small nucleoar box C/D RNPs and the spliceosomal U4 snRNP."
    Watkins N.J., Segault V., Charpentier B., Nottrott S., Fabrizio P., Bachi A., Wilm M., Rosbash M., Branlant C., Luehrmann R.
    Cell 103:457-466(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN U3 SNORNP, IDENTIFICATION BY MASS SPECTROMETRY, RNA-BINDING.
  7. "Composition and functional characterization of the yeast spliceosomal penta-snRNP."
    Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D., Abelson J.
    Mol. Cell 9:31-44(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN U1.U2.U4/U6.U5 PENTA-SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  8. "Purified box C/D snoRNPs are able to reproduce site-specific 2'-O-methylation of target RNA in vitro."
    Galardi S., Fatica A., Bachi A., Scaloni A., Presutti C., Bozzoni I.
    Mol. Cell. Biol. 22:6663-6668(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN U3 SNORNP, IDENTIFICATION BY MASS SPECTROMETRY.
  9. Cited for: IDENTIFICATION IN SSU PROCESSOME BY MASS SPECTROMETRY.
  10. "A structural, phylogenetic, and functional study of 15.5-kD/Snu13 protein binding on U3 small nucleolar RNA."
    Marmier-Gourrier N., Clery A., Senty-Segault V., Charpentier B., Schlotter F., Leclerc F., Fournier R., Branlant C.
    RNA 9:821-838(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: RNA-BINDING.
  11. "Analysis of Snu13p mutations reveals differential interactions with the U4 snRNA and U3 snoRNA."
    Dobbyn H.C., O'Keefe R.T.
    RNA 10:308-320(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF GLU-59; VAL-81 AND ARG-84, RNA-BINDING.
  12. "Structural comparison of yeast snoRNP and spliceosomal protein Snu13p with its homologs."
    Oruganti S., Zhang Y., Li H.
    Biochem. Biophys. Res. Commun. 333:550-554(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).

Entry informationi

Entry nameiSNU13_YEAST
AccessioniPrimary (citable) accession number: P39990
Secondary accession number(s): D3DLM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3