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P39990 (SNU13_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
13 kDa ribonucleoprotein-associated protein
Alternative name(s):
Small nuclear ribonucleoprotein-associated protein 1
Gene names
Name:SNU13
Ordered Locus Names:YEL026W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length126 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Common component of the spliceosome and rRNA processing machinery. In association with the spliceosomal U4/U6.U5 tri-snRNP particle, required for splicing of pre-mRNA. In association with box C/D snoRNPs, required for processing of pre-ribosomal RNA (rRNA) and site-specific 2'-O-methylation of substrate RNAs. Essential for the accumulation and stability of U4 snRNA, U6 snRNA, and box C/D snoRNAs. Ref.6 Ref.8 Ref.11

Subunit structure

Binds to the C'/D and B/C motifs in U3 snoRNA. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1. Binds to the 5'-stem-loop of U4 snRNA. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9

Subcellular location

Nucleusnucleolus.

Sequence similarities

Belongs to the ribosomal protein L7Ae family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12612613 kDa ribonucleoprotein-associated protein
PRO_0000136777

Experimental info

Mutagenesis591E → A: Impairs binding to U4 snRNA, but not U3 snoRNA. Causes pre-mRNA splicing and pre-rRNA processing defects. Ref.11
Mutagenesis811V → L: Impairs binding to U4 snRNA, but not U3 snoRNA, and causes pre rRNA processing defects and an accumulation of unspliced U3 snoRNA; when associated with A-84. Ref.11
Mutagenesis841R → A: Impairs binding to U4 snRNA, but not U3 snoRNA, and causes pre rRNA processing defects and an accumulation of unspliced U3 snoRNA; when associated with L-81. Ref.11

Secondary structure

................... 126
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P39990 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 4489780153E8F182

FASTA12613,569
        10         20         30         40         50         60 
MSAPNPKAFP LADAALTQQI LDVVQQAANL RQLKKGANEA TKTLNRGISE FIIMAADCEP 

        70         80         90        100        110        120 
IEILLHLPLL CEDKNVPYVF VPSRVALGRA CGVSRPVIAA SITTNDASAI KTQIYAVKDK 


IETLLI 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP."
Gottschalk A., Neubauer G., Banroques J., Mann M., Luehrmann R., Fabrizio P.
EMBO J. 18:4535-4548(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[5]"Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins."
Stevens S.W., Abelson J.
Proc. Natl. Acad. Sci. U.S.A. 96:7226-7231(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN U4/U6.U5 TRI-SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[6]"A common core RNP structure shared between the small nucleoar box C/D RNPs and the spliceosomal U4 snRNP."
Watkins N.J., Segault V., Charpentier B., Nottrott S., Fabrizio P., Bachi A., Wilm M., Rosbash M., Branlant C., Luehrmann R.
Cell 103:457-466(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN U3 SNORNP, IDENTIFICATION BY MASS SPECTROMETRY, RNA-BINDING.
[7]"Composition and functional characterization of the yeast spliceosomal penta-snRNP."
Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D., Abelson J.
Mol. Cell 9:31-44(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN U1.U2.U4/U6.U5 PENTA-SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[8]"Purified box C/D snoRNPs are able to reproduce site-specific 2'-O-methylation of target RNA in vitro."
Galardi S., Fatica A., Bachi A., Scaloni A., Presutti C., Bozzoni I.
Mol. Cell. Biol. 22:6663-6668(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN U3 SNORNP, IDENTIFICATION BY MASS SPECTROMETRY.
[9]"A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis."
Dragon F., Gallagher J.E.G., Compagnone-Post P.A., Mitchell B.M., Porwancher K.A., Wehner K.A., Wormsley S., Settlage R.E., Shabanowitz J., Osheim Y., Beyer A.L., Hunt D.F., Baserga S.J.
Nature 417:967-970(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN SSU PROCESSOME BY MASS SPECTROMETRY.
[10]"A structural, phylogenetic, and functional study of 15.5-kD/Snu13 protein binding on U3 small nucleolar RNA."
Marmier-Gourrier N., Clery A., Senty-Segault V., Charpentier B., Schlotter F., Leclerc F., Fournier R., Branlant C.
RNA 9:821-838(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: RNA-BINDING.
[11]"Analysis of Snu13p mutations reveals differential interactions with the U4 snRNA and U3 snoRNA."
Dobbyn H.C., O'Keefe R.T.
RNA 10:308-320(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF GLU-59; VAL-81 AND ARG-84, RNA-BINDING.
[12]"Structural comparison of yeast snoRNP and spliceosomal protein Snu13p with its homologs."
Oruganti S., Zhang Y., Li H.
Biochem. Biophys. Res. Commun. 333:550-554(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U18530 Genomic DNA. Translation: AAB64503.1.
AY558377 Genomic DNA. Translation: AAS56703.1.
BK006939 Genomic DNA. Translation: DAA07626.1.
PIRS50433.
RefSeqNP_010888.1. NM_001178841.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZWZX-ray1.90A/B1-126[»]
2ALEX-ray1.80A1-126[»]
ProteinModelPortalP39990.
SMRP39990. Positions 1-126.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36703. 56 interactions.
DIPDIP-2870N.
IntActP39990. 18 interactions.
MINTMINT-627070.
STRING4932.YEL026W.

Proteomic databases

MaxQBP39990.
PaxDbP39990.
PeptideAtlasP39990.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYEL026W; YEL026W; YEL026W.
GeneID856687.
KEGGsce:YEL026W.

Organism-specific databases

CYGDYEL026w.
SGDS000000752. SNU13.

Phylogenomic databases

eggNOGCOG1358.
GeneTreeENSGT00550000074840.
HOGENOMHOG000055226.
KOK12845.
OMAQWIPLEN.
OrthoDBEOG790GCX.

Enzyme and pathway databases

BioCycYEAST:G3O-30149-MONOMER.

Gene expression databases

GenevestigatorP39990.

Family and domain databases

Gene3D3.30.1330.30. 1 hit.
InterProIPR002415. H/ACA_rnp_Nhp2_euk.
IPR029064. L30e-like.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR018492. Ribosomal_L7Ae/L8/Nhp2.
IPR004037. Ribosomal_L7Ae_CS.
[Graphical view]
PfamPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
PRINTSPR00881. L7ARS6FAMILY.
PR00883. NUCLEARHMG.
SUPFAMSSF55315. SSF55315. 1 hit.
PROSITEPS01082. RIBOSOMAL_L7AE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP39990.
NextBio982724.
PROP39990.

Entry information

Entry nameSNU13_YEAST
AccessionPrimary (citable) accession number: P39990
Secondary accession number(s): D3DLM2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references