13 kDa ribonucleoprotein-associated protein - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P39990 (SNU13_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 115. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
13 kDa ribonucleoprotein-associated protein

Alternative name(s):
Small nuclear ribonucleoprotein-associated protein 1

Gene names

Name:	SNU13
Ordered Locus Names:	YEL026W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	126 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Common component of the spliceosome and rRNA processing machinery. In association with the spliceosomal U4/U6.U5 tri-snRNP particle, required for splicing of pre-mRNA. In association with box C/D snoRNPs, required for processing of pre-ribosomal RNA (rRNA) and site-specific 2'-O-methylation of substrate RNAs. Essential for the accumulation and stability of U4 snRNA, U6 snRNA, and box C/D snoRNAs. Ref.6 Ref.8 Ref.11
Subunit structure	Binds to the C'/D and B/C motifs in U3 snoRNA. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1. Binds to the 5'-stem-loop of U4 snRNA. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9
Subcellular location	Nucleus › nucleolus.
Sequence similarities	Belongs to the ribosomal protein L7Ae family.

Ontologies

Keywords
Biological process	Ribosome biogenesis mRNA processing mRNA splicing rRNA processing
Cellular component	Nucleus Spliceosome
Ligand	RNA-binding
Molecular function	Ribonucleoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA splicing, via spliceosome Inferred from mutant phenotype PubMed 11720284 Ref.11. Source: SGD maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.11. Source: SGD
Cellular_component	U4/U6 x U5 tri-snRNP complex Inferred from direct assay Ref.5 Ref.4. Source: SGD box C/D snoRNP complex Inferred from direct assay Ref.6. Source: SGD small-subunit processome Inferred from direct assay Ref.9. Source: SGD spliceosomal complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	RNA binding Inferred from direct assay PubMed 17332014. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 126

126

13 kDa ribonucleoprotein-associated protein

PRO_0000136777

Experimental info

Mutagenesis

E → A: Impairs binding to U4 snRNA, but not U3 snoRNA. Causes pre-mRNA splicing and pre-rRNA processing defects. Ref.11

Mutagenesis

V → L: Impairs binding to U4 snRNA, but not U3 snoRNA, and causes pre rRNA processing defects and an accumulation of unspliced U3 snoRNA; when associated with A-84. Ref.11

Mutagenesis

R → A: Impairs binding to U4 snRNA, but not U3 snoRNA, and causes pre rRNA processing defects and an accumulation of unspliced U3 snoRNA; when associated with L-81. Ref.11

Secondary structure

126

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P39990 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 4489780153E8F182
Show »

FASTA

126

13,569

        10         20         30         40         50         60 
MSAPNPKAFP LADAALTQQI LDVVQQAANL RQLKKGANEA TKTLNRGISE FIIMAADCEP 

        70         80         90        100        110        120 
IEILLHLPLL CEDKNVPYVF VPSRVALGRA CGVSRPVIAA SITTNDASAI KTQIYAVKDK 


IETLLI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP." Gottschalk A., Neubauer G., Banroques J., Mann M., Luehrmann R., Fabrizio P. EMBO J. 18:4535-4548(1999) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, MASS SPECTROMETRY.
[5]	"Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins." Stevens S.W., Abelson J. Proc. Natl. Acad. Sci. U.S.A. 96:7226-7231(1999) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN U4/U6.U5 TRI-SNRNP COMPLEX, MASS SPECTROMETRY.
[6]	"A common core RNP structure shared between the small nucleoar box C/D RNPs and the spliceosomal U4 snRNP." Watkins N.J., Segault V., Charpentier B., Nottrott S., Fabrizio P., Bachi A., Wilm M., Rosbash M., Branlant C., Luehrmann R. Cell 103:457-466(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN U3 SNORNP, MASS SPECTROMETRY, RNA-BINDING.
[7]	"Composition and functional characterization of the yeast spliceosomal penta-snRNP." Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D., Abelson J. Mol. Cell 9:31-44(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN U1.U2.U4/U6.U5 PENTA-SNRNP COMPLEX, MASS SPECTROMETRY.
[8]	"Purified box C/D snoRNPs are able to reproduce site-specific 2'-O-methylation of target RNA in vitro." Galardi S., Fatica A., Bachi A., Scaloni A., Presutti C., Bozzoni I. Mol. Cell. Biol. 22:6663-6668(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN U3 SNORNP, MASS SPECTROMETRY.
[9]	"A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis." Dragon F., Gallagher J.E.G., Compagnone-Post P.A., Mitchell B.M., Porwancher K.A., Wehner K.A., Wormsley S., Settlage R.E., Shabanowitz J., Osheim Y., Beyer A.L., Hunt D.F., Baserga S.J. Nature 417:967-970(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN SSU PROCESSOME BY MASS SPECTROMETRY.
[10]	"A structural, phylogenetic, and functional study of 15.5-kD/Snu13 protein binding on U3 small nucleolar RNA." Marmier-Gourrier N., Clery A., Senty-Segault V., Charpentier B., Schlotter F., Leclerc F., Fournier R., Branlant C. RNA 9:821-838(2003) [PubMed] [Europe PMC] [Abstract] Cited for: RNA-BINDING.
[11]	"Analysis of Snu13p mutations reveals differential interactions with the U4 snRNA and U3 snoRNA." Dobbyn H.C., O'Keefe R.T. RNA 10:308-320(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF GLU-59; VAL-81 AND ARG-84, RNA-BINDING.
[12]	"Structural comparison of yeast snoRNP and spliceosomal protein Snu13p with its homologs." Oruganti S., Zhang Y., Li H. Biochem. Biophys. Res. Commun. 333:550-554(2005) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U18530 Genomic DNA. Translation: AAB64503.1.
AY558377 Genomic DNA. Translation: AAS56703.1.
BK006939 Genomic DNA. Translation: DAA07626.1.

PIR

S50433.

RefSeq

NP_010888.1. NM_001178841.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZWZ	X-ray	1.90	A/B	1-126	[»]
2ALE	X-ray	1.80	A	1-126	[»]

ProteinModelPortal

P39990.

SMR

P39990. Positions 1-126.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-2870N.

IntAct

P39990. 16 interactions.

MINT

MINT-627070.

STRING

4932.YEL026W.

Proteomic databases

PaxDb

P39990.

PeptideAtlas

P39990.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YEL026W; YEL026W; YEL026W.

GeneID

856687.

KEGG

sce:YEL026W.

Organism-specific databases

CYGD

YEL026w.

SGD

S000000752. SNU13.

Phylogenomic databases

eggNOG

COG1358.

GeneTree

ENSGT00550000074840.

HOGENOM

HOG000055226.

K12845.

OMA

QASNYKQ.

OrthoDB

EOG4VQDZT.

Gene expression databases

Genevestigator

P39990.

GermOnline

YEL026W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR002415. H/ACA_rnp_Nhp2_euk.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR018492. Ribosomal_L7Ae/L8/Nhp2.
IPR004037. Ribosomal_L7Ae_CS.
[Graphical view]

Pfam

PF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]

PRINTS

PR00881. L7ARS6FAMILY.
PR00883. NUCLEARHMG.

PROSITE

PS01082. RIBOSOMAL_L7AE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P39990.

NextBio

982724.

Entry information

Entry name

SNU13_YEAST

Accession

Primary (citable) accession number: P39990
Secondary accession number(s): D3DLM2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents