Reviewed, UniProtKB/Swiss-Prot P39988 (BUD16_YEAST)

Names and origin

Protein names

Recommended name:
    Putative pyridoxal kinase BUD16
    EC=2.7.1.35
Alternative name(s):
    Bud site selection protein 16

Gene names

Name:	BUD16
Ordered Locus Names:	YEL029C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for synthesis of pyridoxal-5-phosphate from vitamin B6 By similarity. Important for bud site selection.
Catalytic activity	ATP + pyridoxal = ADP + pyridoxal 5'-phosphate.
Cofactor	Divalent cations By similarity.
Subcellular location	Cytoplasm. Nucleus.
Sequence similarities	Belongs to the pyridoxine kinase family.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular bud site selection Ref.2 Inferred from mutant phenotype. Source: SGD pyridoxal phosphate biosynthetic process Inferred from genetic interaction. Source: SGD pyridoxine biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pyridoxal kinase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Putative pyridoxal kinase BUD16		PRO_0000213348
Regions
	Nucleotide binding	183 – 184	2	ATP By similarity
	Nucleotide binding	211 – 223	13	ATP By similarity
Sites
	Binding site	9	1	Substrate By similarity
	Binding site	44	1	Substrate By similarity
	Binding site	122	1	Substrate By similarity
	Binding site	224	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P39988-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 0A6C10767DE34073	FASTA	312	35,559
	10 20 30 40 50 60 MPRLLATQSH VVHGYVGNKA ATFPLQCLGW DVDCCNSVQF SNHTGYGLDK VFGTITRETD 70 80 90 100 110 120 LKELLSGLFD NFSQDYQALL SGYLPNKNSV RCMGTYYAKF KEANPEMIWL MDPVMGDEGQ 130 140 150 160 170 180 LYVSEDVIPE YRKLALSPKQ LVDIITPNQF ELEILYGGEI KTKEHLKKAL KKLHQTIPVI 190 200 210 220 230 240 IVTSCDCKMF DDKDFIYCVA SMEGKTPIVY RVPFIDSYFT GVGDLFSALL LDRVYKILSN 250 260 270 280 290 300 PTTTLKFEDQ VNNVLNVIQK VLKITRSYAS GKMKAKMGSA LEMKEMELRL IESRDIYETI 310 NIHQTDYIYA RL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae." Ni L., Snyder M. Mol. Biol. Cell 12:2147-2170(2001) [PubMed: 11452010] [Abstract] Cited for: FUNCTION.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U18530 Genomic DNA. Translation: AAB64506.1.
PIR	S50430.
RefSeq	NP_010885.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P39988. 2 interactions.
STRING	P39988.
Genome annotation databases
Ensembl	YEL029C; YEL029C; YEL029C; Saccharomyces cerevisiae. [Genome view]
GeneID	856683.
KEGG	sce:YEL029C.
NMPDR	fig|4932.3.peg.1939.
Organism-specific databases
CYGD	YEL029c.
SGD	S000000755. BUD16.
Phylogenomic databases
HOGENOM	P39988.
OMA	GLEDEKA
OrthoDB	EOG9N5XCZ
Gene expression databases
ArrayExpress	P39988.
Genevestigator	P39988.
GermOnline	YEL029C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011611. Carb/pur_kinase. IPR004625. PyrdxlP_synth_PyrdxlKinase. [Graphical view]
Pfam	PF00294. PfkB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00687. pyridox_kin. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	982716.

Entry information

Entry name

BUD16_YEAST

Accession

Primary (citable) accession number: P39988

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 24, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)