Reviewed, UniProtKB/Swiss-Prot P39987 (HSP7E_YEAST)

Names and origin

Protein names

Recommended name:
    Heat shock protein SSC3, mitochondrial
Alternative name(s):
    Extracellular mutant protein 10

Gene names

Name:	ECM10
Synonyms:	SSC3
Ordered Locus Names:	YEL030W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	644 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays a role in facilitating the assembly of some protein complexes inside the mitochondria. It may initiate the events that lead to refolding of imported precursors in the matrix space. Ref.2
Subcellular location	Mitochondrion matrix › mitochondrion nucleoid.
Sequence similarities	Belongs to the heat shock protein 70 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein refolding Ref.2 Inferred from genetic interaction. Source: SGD protein targeting to mitochondrion Ref.2 Inferred from genetic interaction. Source: SGD
Cellular component	mitochondrial nucleoid Ref.3 Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW unfolded protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC13	P32797	1	EBI-22339,EBI-4187

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 644		Heat shock protein SSC3, mitochondrial		PRO_0000013554

Sequences

	Sequence		Length	Mass (Da)
	P39987-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 6A248DE208F513CB	FASTA	644	70,084
	10 20 30 40 50 60 MLPSWKAFKA HNILRILTRF QSTKIPDAVI GIDLGTTNSA VAIMEGKVPR IIENAEGSRT 70 80 90 100 110 120 TPSVVAFTKD GERLVGEPAK RQSVINSENT LFATKRLIGR RFEDAEVQRD INQVPFKIVK 130 140 150 160 170 180 HSNGDAWVEA RNRTYSPAQI GGFILNKMKE TAEAYLAKSV KNAVVTVPAY FNDAQRQATK 190 200 210 220 230 240 DAGQIIGLNV LRVVNEPTAA ALAYGLDKSE PKVIAVFDLG GGTFDISILD IDNGIFEVKS 250 260 270 280 290 300 TNGDTHLGGE DFDIYLLQEI ISHFKKETGI DLSNDRMAVQ RIREAAEKAK IELSSTLSTE 310 320 330 340 350 360 INLPFITADA AGPKHIRMPF SRVQLENITA PLIDRTVDPV KKALKDARIT ASDISDVLLV 370 380 390 400 410 420 GGMSRMPKVA DTVKKLFGKD ASKAVNPDEA VALGAAIQAA VLSGEVTDVL LLDVTPLSLG 430 440 450 460 470 480 IETLGGVFTK LIPRNSTIPN KKSQIFSTAA SGQTSVEVKV FQGERELVKD NKLIGNFTLA 490 500 510 520 530 540 GIPPAPKGTP QIEVTFDIDA NGIINVSAKD LASHKDSSIT VAGASGLSDT EIDRMVNEAE 550 560 570 580 590 600 RYKNQDRARR NAIETANKAD QLANDTENSI KEFEGKLDKT DSQRLKDQIS SLRELVSRSQ 610 620 630 640 AGDEVNDDDV GTKIDNLRTS SMKLFEQLYK NSDNPETKNG RENK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Ecm10, a novel hsp70 homolog in the mitochondrial matrix of the yeast Saccharomyces cerevisiae." Baumann F., Milisav I., Neupert W., Herrmann J.M. FEBS Lett. 487:307-312(2000) [PubMed: 11150530] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[3]	"Ecm10p localizes in yeast mitochondrial nucleoids and its overexpression induces extensive mitochondrial DNA aggregations." Sakasegawa Y., Hachiya N.S., Tsukita S., Kaneko K. Biochem. Biophys. Res. Commun. 309:217-221(2003) [PubMed: 12943685] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U18530 Genomic DNA. Translation: AAB64507.1.
PIR	S50429.
RefSeq	NP_010884.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6541N.
IntAct	P39987. 16 interactions.
STRING	P39987.
Proteomic databases
PeptideAtlas	P39987.
PRIDE	P39987.
Genome annotation databases
Ensembl	YEL030W; YEL030W; YEL030W; Saccharomyces cerevisiae. [Genome view]
GeneID	856682.
KEGG	sce:YEL030W.
NMPDR	fig|4932.3.peg.1938.
Organism-specific databases
CYGD	YEL030w.
SGD	S000000756. ECM10.
Phylogenomic databases
HOGENOM	P39987.
OMA	LENITAP
OrthoDB	EOG9M938X
Gene expression databases
ArrayExpress	P39987.
Genevestigator	P39987.
GermOnline	YEL030W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR012725. Chaperone_DnaK. IPR018181. Heat_shock_70_CS. IPR001023. Hsp70. IPR013126. Hsp_70. [Graphical view]
PANTHER	PTHR19375. Hsp70. 1 hit.
Pfam	PF00012. HSP70. 1 hit. [Graphical view]
PRINTS	PR00301. HEATSHOCK70.
TIGRFAMs	TIGR02350. prok_dnaK. 1 hit.
PROSITE	PS00297. HSP70_1. 1 hit. PS00329. HSP70_2. 1 hit. PS01036. HSP70_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	982713.

Entry information

Entry name

HSP7E_YEAST

Accession

Primary (citable) accession number: P39987

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 24, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)