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P39986

- ATC6_YEAST

UniProt

P39986 - ATC6_YEAST

Protein

Manganese-transporting ATPase 1

Gene

SPF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Mediates manganese transport into the endoplasmic reticulum. The ATPase activity is required for cellular manganese homeostasis.1 Publication

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei487 – 48714-aspartylphosphate intermediateBy similarity
    Metal bindingi816 – 8161MagnesiumBy similarity
    Metal bindingi820 – 8201MagnesiumBy similarity

    GO - Molecular functioni

    1. ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism Source: SGD
    2. ATP binding Source: UniProtKB-KW
    3. cation-transporting ATPase activity Source: InterPro
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cellular calcium ion homeostasis Source: SGD
    2. cellular manganese ion homeostasis Source: SGD
    3. ion transmembrane transport Source: GOC
    4. sterol homeostasis Source: SGD
    5. transmembrane transport Source: SGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Transport

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30153-MONOMER.

    Protein family/group databases

    TCDBi3.A.3.10.3. the p-type atpase (p-atpase) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Manganese-transporting ATPase 1 (EC:3.6.3.-)
    Gene namesi
    Name:SPF1
    Ordered Locus Names:YEL031W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYEL031w.
    SGDiS000000757. SPF1.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. cis-Golgi network Source: SGD
    2. endoplasmic reticulum membrane Source: SGD
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Specific reduction in the luminal concentration of manganese ions, and increase of cytosolic Mn2+-dependent processes. Interruption of sphingolipids biosynthesis, reduced exit of GPI-anchored proteins from the ER, reduced protein mannosylation. Overexpression leads to an increase of luminal manganese.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi487 – 4871D → N: Loss of ATPase activity and transport function. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12151215Manganese-transporting ATPase 1PRO_0000046349Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei324 – 3241Phosphoserine1 Publication
    Modified residuei936 – 9361Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP39986.
    PaxDbiP39986.
    PeptideAtlasiP39986.

    Expressioni

    Gene expression databases

    GenevestigatoriP39986.

    Interactioni

    Protein-protein interaction databases

    BioGridi36698. 324 interactions.
    DIPiDIP-6637N.
    IntActiP39986. 3 interactions.
    MINTiMINT-633403.
    STRINGi4932.YEL031W.

    Structurei

    3D structure databases

    ProteinModelPortaliP39986.
    SMRiP39986. Positions 225-865, 939-999.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2121CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini44 – 496ExtracellularSequence Analysis
    Topological domaini73 – 191119CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini215 – 2173ExtracellularSequence Analysis
    Topological domaini237 – 399163CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini420 – 43213ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini455 – 993539CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1014 – 10207ExtracellularSequence Analysis
    Topological domaini1038 – 105518CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1080 – 109920ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1123 – 113311CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1154 – 117017ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1194 – 121522CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei22 – 4322HelicalSequence AnalysisAdd
    BLAST
    Transmembranei50 – 7223HelicalSequence AnalysisAdd
    BLAST
    Transmembranei192 – 21423HelicalSequence AnalysisAdd
    BLAST
    Transmembranei218 – 23619HelicalSequence AnalysisAdd
    BLAST
    Transmembranei400 – 41920HelicalSequence AnalysisAdd
    BLAST
    Transmembranei433 – 45422HelicalSequence AnalysisAdd
    BLAST
    Transmembranei994 – 101320HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1021 – 103717HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1056 – 107924HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1100 – 112223HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1134 – 115320HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1171 – 119323HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0474.
    GeneTreeiENSGT00550000075064.
    HOGENOMiHOG000199432.
    KOiK14950.
    OMAiIISCPLK.
    OrthoDBiEOG74R1ZZ.

    Family and domain databases

    Gene3Di2.70.150.10. 1 hit.
    3.40.1110.10. 1 hit.
    3.40.50.1000. 2 hits.
    InterProiIPR006544. ATPase_P-typ_Cation_typ_V.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view]
    PANTHERiPTHR24093:SF82. PTHR24093:SF82. 1 hit.
    PfamiPF00122. E1-E2_ATPase. 1 hit.
    [Graphical view]
    PRINTSiPR00119. CATATPASE.
    SUPFAMiSSF56784. SSF56784. 3 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsiTIGR01494. ATPase_P-type. 2 hits.
    TIGR01657. P-ATPase-V. 1 hit.
    PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39986-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTKKSFVSSP IVRDSTLLVP KSLIAKPYVL PFFPLYATFA QLYFQQYDRY     50
    IKGPEWTFVY LGTLVSLNIL VMLMPAWNVK IKAKFNYSTT KNVNEATHIL 100
    IYTTPNNGSD GIVEIQRVTE AGSLQTFFQF QKKRFLWHEN EQVFSSPKFL 150
    VDESPKIGDF QKCKGHSGDL THLKRLYGEN SFDIPIPTFM ELFKEHAVAP 200
    LFVFQVFCVA LWLLDEFWYY SLFNLFMIIS MEAAAVFQRL TALKEFRTMG 250
    IKPYTINVFR NKKWVALQTN ELLPMDLVSI TRTAEESAIP CDLILLDGSA 300
    IVNEAMLSGE STPLLKESIK LRPSEDNLQL DGVDKIAVLH GGTKALQVTP 350
    PEHKSDIPPP PDGGALAIVT KTGFETSQGS LVRVMIYSAE RVSVDNKEAL 400
    MFILFLLIFA VIASWYVWVE GTKMGRIQSK LILDCILIIT SVVPPELPME 450
    LTMAVNSSLA ALAKFYVYCT EPFRIPFAGR IDVCCFDKTG TLTGEDLVFE 500
    GLAGISADSE NIRHLYSAAE APESTILVIG AAHALVKLED GDIVGDPMEK 550
    ATLKAVGWAV ERKNSNYREG TGKLDIIRRF QFSSALKRSA SIASHNDALF 600
    AAVKGAPETI RERLSDIPKN YDEIYKSFTR SGSRVLALAS KSLPKMSQSK 650
    IDDLNRDDVE SELTFNGFLI FHCPLKDDAI ETIKMLNESS HRSIMITGDN 700
    PLTAVHVAKE VGIVFGETLI LDRAGKSDDN QLLFRDVEET VSIPFDPSKD 750
    TFDHSKLFDR YDIAVTGYAL NALEGHSQLR DLLRHTWVYA RVSPSQKEFL 800
    LNTLKDMGYQ TLMCGDGTND VGALKQAHVG IALLNGTEEG LKKLGEQRRL 850
    EGMKMMYIKQ TEFMARWNQP QPPVPEPIAH LFPPGPKNPH YLKALESKGT 900
    VITPEIRKAV EEANSKPVEV IKPNGLSEKK PADLASLLLN SAGDAQGDEA 950
    PALKLGDASC AAPFTSKLAN VSAVTNIIRQ GRCALVNTIQ MYKILALNCL 1000
    ISAYSLSIIY MAGVKFGDGQ ATVSGLLLSV CFLSISRGKP LEKLSKQRPQ 1050
    SGIFNVYIMG SILSQFAVHI ATLVYITTEI YKLEPREPQV DLEKEFAPSL 1100
    LNTGIFIIQL VQQVSTFAVN YQGEPFRENI RSNKGMYYGL LGVTGLALAS 1150
    ATEFLPELNE AMKFVPMTDD FKIKLTLTLL LDFFGSWGVE HFFKFFFMDD 1200
    KPSDISVQQV KIASK 1215
    Length:1,215
    Mass (Da):135,269
    Last modified:February 1, 1995 - v1
    Checksum:i7A9960D34B91B5AE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18530 Genomic DNA. Translation: AAB64508.1.
    BK006939 Genomic DNA. Translation: DAA07622.1.
    PIRiS50428.
    RefSeqiNP_010883.3. NM_001178846.3.

    Genome annotation databases

    EnsemblFungiiYEL031W; YEL031W; YEL031W.
    GeneIDi856681.
    KEGGisce:YEL031W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18530 Genomic DNA. Translation: AAB64508.1 .
    BK006939 Genomic DNA. Translation: DAA07622.1 .
    PIRi S50428.
    RefSeqi NP_010883.3. NM_001178846.3.

    3D structure databases

    ProteinModelPortali P39986.
    SMRi P39986. Positions 225-865, 939-999.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36698. 324 interactions.
    DIPi DIP-6637N.
    IntActi P39986. 3 interactions.
    MINTi MINT-633403.
    STRINGi 4932.YEL031W.

    Protein family/group databases

    TCDBi 3.A.3.10.3. the p-type atpase (p-atpase) superfamily.

    Proteomic databases

    MaxQBi P39986.
    PaxDbi P39986.
    PeptideAtlasi P39986.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YEL031W ; YEL031W ; YEL031W .
    GeneIDi 856681.
    KEGGi sce:YEL031W.

    Organism-specific databases

    CYGDi YEL031w.
    SGDi S000000757. SPF1.

    Phylogenomic databases

    eggNOGi COG0474.
    GeneTreei ENSGT00550000075064.
    HOGENOMi HOG000199432.
    KOi K14950.
    OMAi IISCPLK.
    OrthoDBi EOG74R1ZZ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30153-MONOMER.

    Miscellaneous databases

    NextBioi 982710.
    PROi P39986.

    Gene expression databases

    Genevestigatori P39986.

    Family and domain databases

    Gene3Di 2.70.150.10. 1 hit.
    3.40.1110.10. 1 hit.
    3.40.50.1000. 2 hits.
    InterProi IPR006544. ATPase_P-typ_Cation_typ_V.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view ]
    PANTHERi PTHR24093:SF82. PTHR24093:SF82. 1 hit.
    Pfami PF00122. E1-E2_ATPase. 1 hit.
    [Graphical view ]
    PRINTSi PR00119. CATATPASE.
    SUPFAMi SSF56784. SSF56784. 3 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsi TIGR01494. ATPase_P-type. 2 hits.
    TIGR01657. P-ATPase-V. 1 hit.
    PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "P-type ATPase spf1 mutants show a novel resistance mechanism for the killer toxin SMKT."
      Suzuki C., Shimma Y.
      Mol. Microbiol. 32:813-823(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-324 AND SER-936, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, MUTAGENESIS OF ASP-487.

    Entry informationi

    Entry nameiATC6_YEAST
    AccessioniPrimary (citable) accession number: P39986
    Secondary accession number(s): D3DLL8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 139 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1870 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3