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P39986

- ATC6_YEAST

UniProt

P39986 - ATC6_YEAST

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Protein
Manganese-transporting ATPase 1
Gene
SPF1, YEL031W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Mediates manganese transport into the endoplasmic reticulum. The ATPase activity is required for cellular manganese homeostasis.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei487 – 48714-aspartylphosphate intermediate By similarity
Metal bindingi816 – 8161Magnesium By similarity
Metal bindingi820 – 8201Magnesium By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism Source: SGD
  3. cation-transporting ATPase activity Source: InterPro
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cellular calcium ion homeostasis Source: SGD
  2. cellular manganese ion homeostasis Source: SGD
  3. ion transmembrane transport Source: GOC
  4. sterol homeostasis Source: SGD
  5. transmembrane transport Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30153-MONOMER.

Protein family/group databases

TCDBi3.A.3.10.3. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Manganese-transporting ATPase 1 (EC:3.6.3.-)
Gene namesi
Name:SPF1
Ordered Locus Names:YEL031W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYEL031w.
SGDiS000000757. SPF1.

Subcellular locationi

Endoplasmic reticulum membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2121Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei22 – 4322Helical; Reviewed prediction
Add
BLAST
Topological domaini44 – 496Extracellular Reviewed prediction
Transmembranei50 – 7223Helical; Reviewed prediction
Add
BLAST
Topological domaini73 – 191119Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei192 – 21423Helical; Reviewed prediction
Add
BLAST
Topological domaini215 – 2173Extracellular Reviewed prediction
Transmembranei218 – 23619Helical; Reviewed prediction
Add
BLAST
Topological domaini237 – 399163Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei400 – 41920Helical; Reviewed prediction
Add
BLAST
Topological domaini420 – 43213Extracellular Reviewed prediction
Add
BLAST
Transmembranei433 – 45422Helical; Reviewed prediction
Add
BLAST
Topological domaini455 – 993539Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei994 – 101320Helical; Reviewed prediction
Add
BLAST
Topological domaini1014 – 10207Extracellular Reviewed prediction
Transmembranei1021 – 103717Helical; Reviewed prediction
Add
BLAST
Topological domaini1038 – 105518Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei1056 – 107924Helical; Reviewed prediction
Add
BLAST
Topological domaini1080 – 109920Extracellular Reviewed prediction
Add
BLAST
Transmembranei1100 – 112223Helical; Reviewed prediction
Add
BLAST
Topological domaini1123 – 113311Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei1134 – 115320Helical; Reviewed prediction
Add
BLAST
Topological domaini1154 – 117017Extracellular Reviewed prediction
Add
BLAST
Transmembranei1171 – 119323Helical; Reviewed prediction
Add
BLAST
Topological domaini1194 – 121522Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. cis-Golgi network Source: SGD
  2. endoplasmic reticulum membrane Source: SGD
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Specific reduction in the luminal concentration of manganese ions, and increase of cytosolic Mn2+-dependent processes. Interruption of sphingolipids biosynthesis, reduced exit of GPI-anchored proteins from the ER, reduced protein mannosylation. Overexpression leads to an increase of luminal manganese.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi487 – 4871D → N: Loss of ATPase activity and transport function. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12151215Manganese-transporting ATPase 1
PRO_0000046349Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei324 – 3241Phosphoserine1 Publication
Modified residuei936 – 9361Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39986.
PaxDbiP39986.
PeptideAtlasiP39986.

Expressioni

Gene expression databases

GenevestigatoriP39986.

Interactioni

Protein-protein interaction databases

BioGridi36698. 324 interactions.
DIPiDIP-6637N.
IntActiP39986. 3 interactions.
MINTiMINT-633403.
STRINGi4932.YEL031W.

Structurei

3D structure databases

ProteinModelPortaliP39986.
SMRiP39986. Positions 225-865, 939-999.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
GeneTreeiENSGT00550000075064.
HOGENOMiHOG000199432.
KOiK14950.
OMAiIISCPLK.
OrthoDBiEOG74R1ZZ.

Family and domain databases

Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR006544. ATPase_P-typ_Cation_typ_V.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PANTHERiPTHR24093:SF82. PTHR24093:SF82. 1 hit.
PfamiPF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01494. ATPase_P-type. 2 hits.
TIGR01657. P-ATPase-V. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39986-1 [UniParc]FASTAAdd to Basket

« Hide

MTKKSFVSSP IVRDSTLLVP KSLIAKPYVL PFFPLYATFA QLYFQQYDRY     50
IKGPEWTFVY LGTLVSLNIL VMLMPAWNVK IKAKFNYSTT KNVNEATHIL 100
IYTTPNNGSD GIVEIQRVTE AGSLQTFFQF QKKRFLWHEN EQVFSSPKFL 150
VDESPKIGDF QKCKGHSGDL THLKRLYGEN SFDIPIPTFM ELFKEHAVAP 200
LFVFQVFCVA LWLLDEFWYY SLFNLFMIIS MEAAAVFQRL TALKEFRTMG 250
IKPYTINVFR NKKWVALQTN ELLPMDLVSI TRTAEESAIP CDLILLDGSA 300
IVNEAMLSGE STPLLKESIK LRPSEDNLQL DGVDKIAVLH GGTKALQVTP 350
PEHKSDIPPP PDGGALAIVT KTGFETSQGS LVRVMIYSAE RVSVDNKEAL 400
MFILFLLIFA VIASWYVWVE GTKMGRIQSK LILDCILIIT SVVPPELPME 450
LTMAVNSSLA ALAKFYVYCT EPFRIPFAGR IDVCCFDKTG TLTGEDLVFE 500
GLAGISADSE NIRHLYSAAE APESTILVIG AAHALVKLED GDIVGDPMEK 550
ATLKAVGWAV ERKNSNYREG TGKLDIIRRF QFSSALKRSA SIASHNDALF 600
AAVKGAPETI RERLSDIPKN YDEIYKSFTR SGSRVLALAS KSLPKMSQSK 650
IDDLNRDDVE SELTFNGFLI FHCPLKDDAI ETIKMLNESS HRSIMITGDN 700
PLTAVHVAKE VGIVFGETLI LDRAGKSDDN QLLFRDVEET VSIPFDPSKD 750
TFDHSKLFDR YDIAVTGYAL NALEGHSQLR DLLRHTWVYA RVSPSQKEFL 800
LNTLKDMGYQ TLMCGDGTND VGALKQAHVG IALLNGTEEG LKKLGEQRRL 850
EGMKMMYIKQ TEFMARWNQP QPPVPEPIAH LFPPGPKNPH YLKALESKGT 900
VITPEIRKAV EEANSKPVEV IKPNGLSEKK PADLASLLLN SAGDAQGDEA 950
PALKLGDASC AAPFTSKLAN VSAVTNIIRQ GRCALVNTIQ MYKILALNCL 1000
ISAYSLSIIY MAGVKFGDGQ ATVSGLLLSV CFLSISRGKP LEKLSKQRPQ 1050
SGIFNVYIMG SILSQFAVHI ATLVYITTEI YKLEPREPQV DLEKEFAPSL 1100
LNTGIFIIQL VQQVSTFAVN YQGEPFRENI RSNKGMYYGL LGVTGLALAS 1150
ATEFLPELNE AMKFVPMTDD FKIKLTLTLL LDFFGSWGVE HFFKFFFMDD 1200
KPSDISVQQV KIASK 1215
Length:1,215
Mass (Da):135,269
Last modified:February 1, 1995 - v1
Checksum:i7A9960D34B91B5AE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18530 Genomic DNA. Translation: AAB64508.1.
BK006939 Genomic DNA. Translation: DAA07622.1.
PIRiS50428.
RefSeqiNP_010883.3. NM_001178846.3.

Genome annotation databases

EnsemblFungiiYEL031W; YEL031W; YEL031W.
GeneIDi856681.
KEGGisce:YEL031W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18530 Genomic DNA. Translation: AAB64508.1 .
BK006939 Genomic DNA. Translation: DAA07622.1 .
PIRi S50428.
RefSeqi NP_010883.3. NM_001178846.3.

3D structure databases

ProteinModelPortali P39986.
SMRi P39986. Positions 225-865, 939-999.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36698. 324 interactions.
DIPi DIP-6637N.
IntActi P39986. 3 interactions.
MINTi MINT-633403.
STRINGi 4932.YEL031W.

Protein family/group databases

TCDBi 3.A.3.10.3. the p-type atpase (p-atpase) superfamily.

Proteomic databases

MaxQBi P39986.
PaxDbi P39986.
PeptideAtlasi P39986.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YEL031W ; YEL031W ; YEL031W .
GeneIDi 856681.
KEGGi sce:YEL031W.

Organism-specific databases

CYGDi YEL031w.
SGDi S000000757. SPF1.

Phylogenomic databases

eggNOGi COG0474.
GeneTreei ENSGT00550000075064.
HOGENOMi HOG000199432.
KOi K14950.
OMAi IISCPLK.
OrthoDBi EOG74R1ZZ.

Enzyme and pathway databases

BioCyci YEAST:G3O-30153-MONOMER.

Miscellaneous databases

NextBioi 982710.
PROi P39986.

Gene expression databases

Genevestigatori P39986.

Family and domain databases

Gene3Di 2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProi IPR006544. ATPase_P-typ_Cation_typ_V.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
PANTHERi PTHR24093:SF82. PTHR24093:SF82. 1 hit.
Pfami PF00122. E1-E2_ATPase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
SUPFAMi SSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01494. ATPase_P-type. 2 hits.
TIGR01657. P-ATPase-V. 1 hit.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "P-type ATPase spf1 mutants show a novel resistance mechanism for the killer toxin SMKT."
    Suzuki C., Shimma Y.
    Mol. Microbiol. 32:813-823(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-324 AND SER-936, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, MUTAGENESIS OF ASP-487.

Entry informationi

Entry nameiATC6_YEAST
AccessioniPrimary (citable) accession number: P39986
Secondary accession number(s): D3DLL8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 3, 2014
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1870 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi