Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Manganese-transporting ATPase 1

Gene

SPF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates manganese transport into the endoplasmic reticulum. The ATPase activity is required for cellular manganese homeostasis.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4874-aspartylphosphate intermediateBy similarity1
Metal bindingi816MagnesiumBy similarity1
Metal bindingi820MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cellular calcium ion homeostasis Source: SGD
  • cellular manganese ion homeostasis Source: SGD
  • sterol homeostasis Source: SGD
  • transmembrane transport Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30153-MONOMER.
ReactomeiR-SCE-936837. Ion transport by P-type ATPases.

Protein family/group databases

TCDBi3.A.3.10.3. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Manganese-transporting ATPase 1 (EC:3.6.3.-)
Gene namesi
Name:SPF1
Ordered Locus Names:YEL031W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL031W.
SGDiS000000757. SPF1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 21CytoplasmicSequence analysisAdd BLAST21
Transmembranei22 – 43HelicalSequence analysisAdd BLAST22
Topological domaini44 – 49ExtracellularSequence analysis6
Transmembranei50 – 72HelicalSequence analysisAdd BLAST23
Topological domaini73 – 191CytoplasmicSequence analysisAdd BLAST119
Transmembranei192 – 214HelicalSequence analysisAdd BLAST23
Topological domaini215 – 217ExtracellularSequence analysis3
Transmembranei218 – 236HelicalSequence analysisAdd BLAST19
Topological domaini237 – 399CytoplasmicSequence analysisAdd BLAST163
Transmembranei400 – 419HelicalSequence analysisAdd BLAST20
Topological domaini420 – 432ExtracellularSequence analysisAdd BLAST13
Transmembranei433 – 454HelicalSequence analysisAdd BLAST22
Topological domaini455 – 993CytoplasmicSequence analysisAdd BLAST539
Transmembranei994 – 1013HelicalSequence analysisAdd BLAST20
Topological domaini1014 – 1020ExtracellularSequence analysis7
Transmembranei1021 – 1037HelicalSequence analysisAdd BLAST17
Topological domaini1038 – 1055CytoplasmicSequence analysisAdd BLAST18
Transmembranei1056 – 1079HelicalSequence analysisAdd BLAST24
Topological domaini1080 – 1099ExtracellularSequence analysisAdd BLAST20
Transmembranei1100 – 1122HelicalSequence analysisAdd BLAST23
Topological domaini1123 – 1133CytoplasmicSequence analysisAdd BLAST11
Transmembranei1134 – 1153HelicalSequence analysisAdd BLAST20
Topological domaini1154 – 1170ExtracellularSequence analysisAdd BLAST17
Transmembranei1171 – 1193HelicalSequence analysisAdd BLAST23
Topological domaini1194 – 1215CytoplasmicSequence analysisAdd BLAST22

GO - Cellular componenti

  • cis-Golgi network Source: SGD
  • endoplasmic reticulum membrane Source: SGD
  • integral component of plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Specific reduction in the luminal concentration of manganese ions, and increase of cytosolic Mn2+-dependent processes. Interruption of sphingolipids biosynthesis, reduced exit of GPI-anchored proteins from the ER, reduced protein mannosylation. Overexpression leads to an increase of luminal manganese.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi487D → N: Loss of ATPase activity and transport function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000463491 – 1215Manganese-transporting ATPase 1Add BLAST1215

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei324PhosphoserineCombined sources1
Modified residuei936PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39986.
PRIDEiP39986.

PTM databases

iPTMnetiP39986.

Interactioni

Protein-protein interaction databases

BioGridi36698. 323 interactors.
DIPiDIP-6637N.
IntActiP39986. 3 interactors.
MINTiMINT-633403.

Structurei

3D structure databases

ProteinModelPortaliP39986.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000075064.
HOGENOMiHOG000199432.
InParanoidiP39986.
KOiK14950.
OMAiYLYSEAQ.
OrthoDBiEOG092C0IZK.

Family and domain databases

Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006544. P-type_TPase_V.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF82. PTHR24093:SF82. 3 hits.
PfamiPF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01494. ATPase_P-type. 2 hits.
TIGR01657. P-ATPase-V. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39986-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKKSFVSSP IVRDSTLLVP KSLIAKPYVL PFFPLYATFA QLYFQQYDRY
60 70 80 90 100
IKGPEWTFVY LGTLVSLNIL VMLMPAWNVK IKAKFNYSTT KNVNEATHIL
110 120 130 140 150
IYTTPNNGSD GIVEIQRVTE AGSLQTFFQF QKKRFLWHEN EQVFSSPKFL
160 170 180 190 200
VDESPKIGDF QKCKGHSGDL THLKRLYGEN SFDIPIPTFM ELFKEHAVAP
210 220 230 240 250
LFVFQVFCVA LWLLDEFWYY SLFNLFMIIS MEAAAVFQRL TALKEFRTMG
260 270 280 290 300
IKPYTINVFR NKKWVALQTN ELLPMDLVSI TRTAEESAIP CDLILLDGSA
310 320 330 340 350
IVNEAMLSGE STPLLKESIK LRPSEDNLQL DGVDKIAVLH GGTKALQVTP
360 370 380 390 400
PEHKSDIPPP PDGGALAIVT KTGFETSQGS LVRVMIYSAE RVSVDNKEAL
410 420 430 440 450
MFILFLLIFA VIASWYVWVE GTKMGRIQSK LILDCILIIT SVVPPELPME
460 470 480 490 500
LTMAVNSSLA ALAKFYVYCT EPFRIPFAGR IDVCCFDKTG TLTGEDLVFE
510 520 530 540 550
GLAGISADSE NIRHLYSAAE APESTILVIG AAHALVKLED GDIVGDPMEK
560 570 580 590 600
ATLKAVGWAV ERKNSNYREG TGKLDIIRRF QFSSALKRSA SIASHNDALF
610 620 630 640 650
AAVKGAPETI RERLSDIPKN YDEIYKSFTR SGSRVLALAS KSLPKMSQSK
660 670 680 690 700
IDDLNRDDVE SELTFNGFLI FHCPLKDDAI ETIKMLNESS HRSIMITGDN
710 720 730 740 750
PLTAVHVAKE VGIVFGETLI LDRAGKSDDN QLLFRDVEET VSIPFDPSKD
760 770 780 790 800
TFDHSKLFDR YDIAVTGYAL NALEGHSQLR DLLRHTWVYA RVSPSQKEFL
810 820 830 840 850
LNTLKDMGYQ TLMCGDGTND VGALKQAHVG IALLNGTEEG LKKLGEQRRL
860 870 880 890 900
EGMKMMYIKQ TEFMARWNQP QPPVPEPIAH LFPPGPKNPH YLKALESKGT
910 920 930 940 950
VITPEIRKAV EEANSKPVEV IKPNGLSEKK PADLASLLLN SAGDAQGDEA
960 970 980 990 1000
PALKLGDASC AAPFTSKLAN VSAVTNIIRQ GRCALVNTIQ MYKILALNCL
1010 1020 1030 1040 1050
ISAYSLSIIY MAGVKFGDGQ ATVSGLLLSV CFLSISRGKP LEKLSKQRPQ
1060 1070 1080 1090 1100
SGIFNVYIMG SILSQFAVHI ATLVYITTEI YKLEPREPQV DLEKEFAPSL
1110 1120 1130 1140 1150
LNTGIFIIQL VQQVSTFAVN YQGEPFRENI RSNKGMYYGL LGVTGLALAS
1160 1170 1180 1190 1200
ATEFLPELNE AMKFVPMTDD FKIKLTLTLL LDFFGSWGVE HFFKFFFMDD
1210
KPSDISVQQV KIASK
Length:1,215
Mass (Da):135,269
Last modified:February 1, 1995 - v1
Checksum:i7A9960D34B91B5AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18530 Genomic DNA. Translation: AAB64508.1.
BK006939 Genomic DNA. Translation: DAA07622.1.
PIRiS50428.
RefSeqiNP_010883.3. NM_001178846.3.

Genome annotation databases

EnsemblFungiiYEL031W; YEL031W; YEL031W.
GeneIDi856681.
KEGGisce:YEL031W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18530 Genomic DNA. Translation: AAB64508.1.
BK006939 Genomic DNA. Translation: DAA07622.1.
PIRiS50428.
RefSeqiNP_010883.3. NM_001178846.3.

3D structure databases

ProteinModelPortaliP39986.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36698. 323 interactors.
DIPiDIP-6637N.
IntActiP39986. 3 interactors.
MINTiMINT-633403.

Protein family/group databases

TCDBi3.A.3.10.3. the p-type atpase (p-atpase) superfamily.

PTM databases

iPTMnetiP39986.

Proteomic databases

MaxQBiP39986.
PRIDEiP39986.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYEL031W; YEL031W; YEL031W.
GeneIDi856681.
KEGGisce:YEL031W.

Organism-specific databases

EuPathDBiFungiDB:YEL031W.
SGDiS000000757. SPF1.

Phylogenomic databases

GeneTreeiENSGT00550000075064.
HOGENOMiHOG000199432.
InParanoidiP39986.
KOiK14950.
OMAiYLYSEAQ.
OrthoDBiEOG092C0IZK.

Enzyme and pathway databases

BioCyciYEAST:G3O-30153-MONOMER.
ReactomeiR-SCE-936837. Ion transport by P-type ATPases.

Miscellaneous databases

PROiP39986.

Family and domain databases

Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006544. P-type_TPase_V.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF82. PTHR24093:SF82. 3 hits.
PfamiPF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01494. ATPase_P-type. 2 hits.
TIGR01657. P-ATPase-V. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiATC6_YEAST
AccessioniPrimary (citable) accession number: P39986
Secondary accession number(s): D3DLL8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 159 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1870 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.