Probable cation-transporting ATPase 1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P39986 (ATC6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 124. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Probable cation-transporting ATPase 1
EC=3.6.3.-

Gene names

Name:	SPF1
Ordered Locus Names:	YEL031W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	1215 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + H₂O = ADP + phosphate.
Subcellular location	Membrane; Multi-pass membrane protein.
Miscellaneous	Present with 1870 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily. [View classification]

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular calcium ion homeostasis Inferred from mutant phenotype PubMed 12058017. Source: SGD
Cellular_component	cis-Golgi network Inferred from direct assay PubMed 11791712. Source: SGD endoplasmic reticulum membrane Inferred from direct assay PubMed 12058017. Source: SGD integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD mitochondrion Inferred from direct assay PubMed 14576278 PubMed 16823961. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism Inferred from direct assay PubMed 12058017. Source: SGD cation-transporting ATPase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1215

1215

Probable cation-transporting ATPase 1

PRO_0000046349

Regions

Topological domain

1 – 21

Cytoplasmic Potential

Transmembrane

22 – 43

Helical; Potential

Topological domain

44 – 49

Extracellular Potential

Transmembrane

50 – 72

Helical; Potential

Topological domain

73 – 191

119

Cytoplasmic Potential

Transmembrane

192 – 214

Helical; Potential

Topological domain

215 – 217

Extracellular Potential

Transmembrane

218 – 236

Helical; Potential

Topological domain

237 – 399

163

Cytoplasmic Potential

Transmembrane

400 – 419

Helical; Potential

Topological domain

420 – 432

Extracellular Potential

Transmembrane

433 – 454

Helical; Potential

Topological domain

455 – 993

539

Cytoplasmic Potential

Transmembrane

994 – 1013

Helical; Potential

Topological domain

1014 – 1020

Extracellular Potential

Transmembrane

1021 – 1037

Helical; Potential

Topological domain

1038 – 1055

Cytoplasmic Potential

Transmembrane

1056 – 1079

Helical; Potential

Topological domain

1080 – 1099

Extracellular Potential

Transmembrane

1100 – 1122

Helical; Potential

Topological domain

1123 – 1133

Cytoplasmic Potential

Transmembrane

1134 – 1153

Helical; Potential

Topological domain

1154 – 1170

Extracellular Potential

Transmembrane

1171 – 1193

Helical; Potential

Topological domain

1194 – 1215

Cytoplasmic Potential

Sites

Active site

487

4-aspartylphosphate intermediate By similarity

Metal binding

816

Magnesium By similarity

Metal binding

820

Magnesium By similarity

Amino acid modifications

Modified residue

324

Phosphoserine Ref.7

Modified residue

936

Phosphoserine Ref.6 Ref.7

Sequences

Sequence

Length

Mass (Da)

Tools

P39986 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 7A9960D34B91B5AE
Show »

FASTA

1,215

135,269

        10         20         30         40         50         60 
MTKKSFVSSP IVRDSTLLVP KSLIAKPYVL PFFPLYATFA QLYFQQYDRY IKGPEWTFVY 

        70         80         90        100        110        120 
LGTLVSLNIL VMLMPAWNVK IKAKFNYSTT KNVNEATHIL IYTTPNNGSD GIVEIQRVTE 

       130        140        150        160        170        180 
AGSLQTFFQF QKKRFLWHEN EQVFSSPKFL VDESPKIGDF QKCKGHSGDL THLKRLYGEN 

       190        200        210        220        230        240 
SFDIPIPTFM ELFKEHAVAP LFVFQVFCVA LWLLDEFWYY SLFNLFMIIS MEAAAVFQRL 

       250        260        270        280        290        300 
TALKEFRTMG IKPYTINVFR NKKWVALQTN ELLPMDLVSI TRTAEESAIP CDLILLDGSA 

       310        320        330        340        350        360 
IVNEAMLSGE STPLLKESIK LRPSEDNLQL DGVDKIAVLH GGTKALQVTP PEHKSDIPPP 

       370        380        390        400        410        420 
PDGGALAIVT KTGFETSQGS LVRVMIYSAE RVSVDNKEAL MFILFLLIFA VIASWYVWVE 

       430        440        450        460        470        480 
GTKMGRIQSK LILDCILIIT SVVPPELPME LTMAVNSSLA ALAKFYVYCT EPFRIPFAGR 

       490        500        510        520        530        540 
IDVCCFDKTG TLTGEDLVFE GLAGISADSE NIRHLYSAAE APESTILVIG AAHALVKLED 

       550        560        570        580        590        600 
GDIVGDPMEK ATLKAVGWAV ERKNSNYREG TGKLDIIRRF QFSSALKRSA SIASHNDALF 

       610        620        630        640        650        660 
AAVKGAPETI RERLSDIPKN YDEIYKSFTR SGSRVLALAS KSLPKMSQSK IDDLNRDDVE 

       670        680        690        700        710        720 
SELTFNGFLI FHCPLKDDAI ETIKMLNESS HRSIMITGDN PLTAVHVAKE VGIVFGETLI 

       730        740        750        760        770        780 
LDRAGKSDDN QLLFRDVEET VSIPFDPSKD TFDHSKLFDR YDIAVTGYAL NALEGHSQLR 

       790        800        810        820        830        840 
DLLRHTWVYA RVSPSQKEFL LNTLKDMGYQ TLMCGDGTND VGALKQAHVG IALLNGTEEG 

       850        860        870        880        890        900 
LKKLGEQRRL EGMKMMYIKQ TEFMARWNQP QPPVPEPIAH LFPPGPKNPH YLKALESKGT 

       910        920        930        940        950        960 
VITPEIRKAV EEANSKPVEV IKPNGLSEKK PADLASLLLN SAGDAQGDEA PALKLGDASC 

       970        980        990       1000       1010       1020 
AAPFTSKLAN VSAVTNIIRQ GRCALVNTIQ MYKILALNCL ISAYSLSIIY MAGVKFGDGQ 

      1030       1040       1050       1060       1070       1080 
ATVSGLLLSV CFLSISRGKP LEKLSKQRPQ SGIFNVYIMG SILSQFAVHI ATLVYITTEI 

      1090       1100       1110       1120       1130       1140 
YKLEPREPQV DLEKEFAPSL LNTGIFIIQL VQQVSTFAVN YQGEPFRENI RSNKGMYYGL 

      1150       1160       1170       1180       1190       1200 
LGVTGLALAS ATEFLPELNE AMKFVPMTDD FKIKLTLTLL LDFFGSWGVE HFFKFFFMDD 

      1210 
KPSDISVQQV KIASK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"P-type ATPase spf1 mutants show a novel resistance mechanism for the killer toxin SMKT." Suzuki C., Shimma Y. Mol. Microbiol. 32:813-823(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-936, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-324 AND SER-936, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18530 Genomic DNA. Translation: AAB64508.1. BK006939 Genomic DNA. Translation: DAA07622.1.
PIR	S50428.
RefSeq	NP_010883.3. NM_001178846.3.
3D structure databases
ProteinModelPortal	P39986.
SMR	P39986. Positions 225-865.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6637N.
IntAct	P39986. 3 interactions.
MINT	MINT-633403.
STRING	4932.YEL031W.
Protein family/group databases
TCDB	3.A.3.10.1. P-type ATPase (P-ATPase) superfamily.
Proteomic databases
PaxDb	P39986.
PeptideAtlas	P39986.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YEL031W; YEL031W; YEL031W.
GeneID	856681.
KEGG	sce:YEL027W. sce:YEL031W.
Organism-specific databases
CYGD	YEL031w.
SGD	S000000757. SPF1.
Phylogenomic databases
eggNOG	COG0474.
GeneTree	ENSGT00550000075064.
HOGENOM	HOG000199432.
KO	K02155. K14950.
OMA	HWSVHAH.
OrthoDB	EOG43XZBM.
Gene expression databases
Genevestigator	P39986.
GermOnline	YEL031W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.70.150.10. 1 hit. 3.40.1110.10. 1 hit. 3.40.50.1000. 2 hits.
InterPro	IPR006544. ATPase_P-typ_Cation_typ_V. IPR023299. ATPase_P-typ_cyto_domN. IPR018303. ATPase_P-typ_P_site. IPR008250. ATPase_P-typ_transduc_dom_A. IPR001757. Cation_transp_P_typ_ATPase. IPR023214. HAD-like_dom. [Graphical view]
PANTHER	PTHR24093. PTHR24093. 1 hit. PTHR24093:SF82. PTHR24093:SF82. 1 hit.
Pfam	PF00122. E1-E2_ATPase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE.
SUPFAM	SSF81660. ATPase_cation_domN. 1 hit. SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01494. ATPase_P-type. 2 hits. TIGR01657. P-ATPase-V. 1 hit.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982710.

Entry information

Entry name

ATC6_YEAST

Accession

Primary (citable) accession number: P39986
Secondary accession number(s): D3DLL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 124 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents