P39985 (DPO5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase V
Short name=POL V
EC=2.7.7.7

Gene names

Name:	POL5
Ordered Locus Names:	YEL055C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1022 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Presumed to play a role other than chromosomal DNA replication.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subunit structure	Interacts with FRK1. Ref.7
Miscellaneous	Present with 4490 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the DNA polymerase type-B family.

Ontologies

Keywords
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	rRNA transcription Inferred from mutant phenotype Ref.1. Source: SGD
Cellular component	nucleolus Inferred from direct assay Ref.1. Source: SGD
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from direct assay Ref.1. Source: SGD nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1022	1022	DNA polymerase V		PRO_0000046472
Amino acid modifications
	Modified residue	207	1	Phosphotyrosine Ref.6
Natural variations
	Natural variant	625	1	D → N Lethal. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P39985 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: A9F32B1D15039D18	FASTA	1,022	115,894
	10 20 30 40 50 60 MTGKVNRDLF FKLASDLREE RLHAAVALIK DLSALDLPDD AEEWSYVLNR LIKGLSSDRN 70 80 90 100 110 120 SARLGFSLCL TEVINLAVNM PPGQRPKGLE STNEFLSTLS TILNVNVNEG TKKSMKGKDE 130 140 150 160 170 180 RGILFGKLFG LKSLLNEPLF SEIFVKDLEK GNTEFFIRFT EQLIDLALKK NWIKEPCFFT 190 200 210 220 230 240 LFQTMKMLLP FMDESSAEKI LLIYDKYDLT LTNEGLSTYL LLKYEGDESL IPSVLDLKNP 250 260 270 280 290 300 GWKDNDPLAR GNLPLLTKVL RNSSVIPDAN GGLKETKKQK NTNWNPRLHF VWSVLLPLFG 310 320 330 340 350 360 NGKLENTSHI SKKRKKTNNK KVQNSIQFPE FWKMAVDESF FNEKASSERK YLGFLIIDAA 370 380 390 400 410 420 FKAVPGSYIG FCFSQNVMRT LINQSIDSQR VLNKISQLTL DSIVKACEED SANRLVPCLN 430 440 450 460 470 480 AMLFGPHGSI NFDKLTKSGT VSKLIAIKEL PSTVLAQLLD VFFLQLQDKK GVLSHTLFAL 490 500 510 520 530 540 DSILHIVRAH KVEINDMDIM KPVLRPIVYM AFFKHTSDDL KLEQLHELAK ERLYSILGEL 550 560 570 580 590 600 TINKEIRCKD PEINSWQYLT LKLILDIENS HVGDLINPLD ENLENIKNEA ISCLSKVCRS 610 620 630 640 650 660 RTAQSWGLST LLSMCLVQLY AGDTDSISVI EELCEFSKHE NNSMVGITEI LLSLLAQKKA 670 680 690 700 710 720 LLRKLSLIIW QQFIEEVGLE ELQILLDILK ARENKQGFAQ LFEGEEEFEE IKEENDASED 730 740 750 760 770 780 ESKTGSESES ESESDSDDAD EKDEEDEANE DILNIDKEAT SALVKALNLP DNIVNDKGEV 790 800 810 820 830 840 DLDQLEGLSD DGGDDEDEES MDDEKMMELD DQLSEIFKRR KEALSSISTG NQRKFEVKQS 850 860 870 880 890 900 RENVISFKHR VVDMLAVYVK YCEKLTLANK SEHSNNLGGS LSKLVYFIIP MLKCVNETLD 910 920 930 940 950 960 RPLADKISKL LKGKIFKIKV TAFKDMNKDI ELMDLLKKTH KLMLTSKPGQ HAAVFYSMCS 970 980 990 1000 1010 1020 TSSLFLSKLY VEIGGNDKLD ELIDLYTATT KEWMQKGKCG PNIFIDFINW LSSKKQTVMD KE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The fifth essential DNA polymerase phi in Saccharomyces cerevisiae is localized to the nucleolus and plays an important role in synthesis of rRNA." Shimizu K., Kawasaki Y., Hiraga S., Tawaramoto M., Nakashima N., Sugino A. Proc. Natl. Acad. Sci. U.S.A. 99:9133-9138(2002) [PubMed: 12093911] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ASN-625.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Yeast DNA polymerases and their role at the replication fork." Sugino A. Trends Biochem. Sci. 20:319-323(1995) [PubMed: 7667891] [Abstract] Cited for: IDENTIFICATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-207, MASS SPECTROMETRY.
[7]	"A global protein kinase and phosphatase interaction network in yeast." Breitkreutz A., Choi H., Sharom J.R., Boucher L., Neduva V., Larsen B., Lin Z.Y., Breitkreutz B.J., Stark C., Liu G., Ahn J., Dewar-Darch D., Reguly T., Tang X., Almeida R., Qin Z.S., Pawson T., Gingras A.C., Nesvizhskii A.I., Tyers M. Science 328:1043-1046(2010) [PubMed: 20489023] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH FRK1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB027253 Genomic DNA. Translation: BAA77722.1. U18795 Genomic DNA. Translation: AAB65032.1. BK006939 Genomic DNA. Translation: DAA07599.1.
PIR	S50534.
RefSeq	NP_010859.1. NM_001178870.1.
3D structure databases
ProteinModelPortal	P39985.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6314N.
IntAct	P39985. 17 interactions.
MINT	MINT-650861.
STRING	P39985.
Proteomic databases
PeptideAtlas	P39985.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YEL055C; YEL055C; YEL055C.
GeneID	856655.
KEGG	sce:YEL055C.
NMPDR	fig|4932.3.peg.1908.
Organism-specific databases
CYGD	YEL055c.
SGD	S000000781. POL5.
Phylogenomic databases
eggNOG	fuNOG07725.
GeneTree	EFGT00050000002593.
HOGENOM	HBG397536.
OMA	ERKYWGF.
OrthoDB	EOG4MPMZK.
Gene expression databases
ArrayExpress	P39985.
Genevestigator	P39985.
GermOnline	YEL055C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016024. ARM-type_fold. IPR017964. DNA-dir_DNA_pol_B_CS. IPR007015. DNA_pol_V. [Graphical view]
KO	K02331.
PANTHER	PTHR13213. DNA_pol_V. 1 hit.
Pfam	PF04931. DNA_pol_phi. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS00116. DNA_POLYMERASE_B. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982640.

Entry information

Entry name

DPO5_YEAST

Accession

Primary (citable) accession number: P39985
Secondary accession number(s): D3DLJ5, Q9Y737

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program