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Reviewed, UniProtKB/Swiss-Prot P39984 (HAT2_YEAST)

Last modified June 16, 2009. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Histone acetyltransferase type B subunit 2
    EC=2.3.1.48
Gene names
Name: HAT2
Ordered Locus Names: YEL056W
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length401 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Component of the histone acetylase B (HAT-B) complex. The complex acetylates 'Lys-12' of histone H4 which is required for telomeric silencing. HAT2 is required for high affinity binding of the acetyltransferase to histone H4, for the nuclear location of HAT1 and for the HAT1-HIF1 interaction. Alone, it is unable to bind to H4, requiring HAT1 for high affinity interaction with the histone tail. HAT2 has also a HAT1 independent function in life-span regulation. Ref.5 Ref.6 Ref.7 Ref.8

Catalytic activity

Acetyl-CoA + histone = CoA + acetylhistone.

Subunit structure

Component of the HAT-B complex composed of at least HAT1 and HAT2. In the cytoplasm, this complex binds to the histone H4 tail. In the nucleus, the HAT-B complex has an additional component, the histone H3/H4 chaperone HIF1.

Subcellular location

Cytoplasm. Nucleus. Note: The nuclear location requires the presence of HAT2. Ref.6 Ref.7

Induction

Repressed in presence of farnesol, probably through a intracellular decrease of diacylglycerol. Ref.4

Sequence similarities

Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.

Contains 6 WD repeats.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

HAT1Q123411EBI-8185,EBI-8176

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 401401Histone acetyltransferase type B subunit 2
PRO_0000051014

Regions

Repeat116 – 14732WD 1
Repeat158 – 18932WD 2
Repeat206 – 23732WD 3
Repeat249 – 28032WD 4
Repeat293 – 32432WD 5
Repeat350 – 38132WD 6

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Sequences

Sequence LengthMass (Da)Tools
P39984-1 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: C27A91C99D9FCAB7

FASTA40145,060
        10         20         30         40         50         60 
MENQEKPLSV DEEYDLWKSN VPLMYDFVSE TRLTWPSLTV QWLPTPVQEL DGGFIKQELI 

        70         80         90        100        110        120 
IGTHTSGEEE NYLKFAEINL PKEILSNEDP QEEAGEEYQS SLPAPRSNIR ITAKYEHEEE 

       130        140        150        160        170        180 
ITRARYMPQD PNIVATINGQ GTVFLYSRSE GLQSTLKFHK DNGYALSFST LVKGRLLSGS 

       190        200        210        220        230        240 
DDHTVALWEV GSGGDPTKPV RTWNDLHSDI INDNKWHNFN KDLFGTVSED SLLKINDVRA 

       250        260        270        280        290        300 
NNTTIDTVKC PQPFNTLAFS HHSSNLLAAA GMDSYVYLYD LRNMKEPLHH MSGHEDAVNN 

       310        320        330        340        350        360 
LEFSTHVDGV VVSSGSDNRL MMWDLKQIGA EQTPDDAEDG VPELIMVHAG HRSSVNDFDL 

       370        380        390        400 
NPQIPWLVAS AEEENILQVW KCSHSLPIVG GPPKVNKDII S

« Hide

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References

« Hide 'large scale' references
[1]"The major cytoplasmic histone acetyltransferase in yeast: links to chromatin replication and histone metabolism."
Parthun M.R., Widom J., Gottschling D.E.
Cell 87:85-94(1996) [PubMed: 8858151] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 83-105, ACETYLATION OF HISTONE H4, INTERACTION WITH HAT1 AND HISTONE H4.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed: 9169868] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]"HAT1 and HAT2 proteins are components of a yeast nuclear histone acetyltransferase enzyme specific for free histone H4."
Ruiz-Garcia A.B., Sendra R., Galiana M., Pamblanco M., Perez-Ortin J.E., Tordera V.
J. Biol. Chem. 273:12599-12605(1998) [PubMed: 9575221] [Abstract]
Cited for: ACETYLATION OF HISTONE H4 BY THE HAT-B COMPLEX.
[4]"Farnesol-induced growth inhibition in Saccharomyces cerevisiae by a cell cycle mechanism."
Machida K., Tanaka T., Yano Y., Otani S., Taniguchi M.
Microbiology 145:293-299(1999) [PubMed: 10075411] [Abstract]
Cited for: INDUCTION.
[5]"Type B histone acetyltransferase Hat1p participates in telomeric silencing."
Kelly T.J., Qin S., Gottschling D.E., Parthun M.R.
Mol. Cell. Biol. 20:7051-7058(2000) [PubMed: 10982821] [Abstract]
Cited for: FUNCTION, ACETYLATION OF HISTONE H4.
[6]"Hif1 is a component of yeast histone acetyltransferase B, a complex mainly localized in the nucleus."
Poveda A., Pamblanco M., Tafrov S., Tordera V., Sternglanz R., Sendra R.
J. Biol. Chem. 279:16033-16043(2004) [PubMed: 14761951] [Abstract]
Cited for: IDENTIFICATION IN THE HAT-B COMPLEX, FUNCTION OF THE HAT-B COMPLEX, INTERACTION WITH HISTONE H4, SUBCELLULAR LOCATION.
[7]"The nuclear Hat1p/Hat2p complex: a molecular link between type B histone acetyltransferases and chromatin assembly."
Ai X., Parthun M.R.
Mol. Cell 14:195-205(2004) [PubMed: 15099519] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN THE HAT-B COMPLEX, MASS SPECTROMETRY, INTERACTION WITH HISTONES H3 AND H4, SUBCELLULAR LOCATION.
[8]"Yeast HAT1 and HAT2 deletions have different life-span and transcriptome phenotypes."
Rosaleny L.E., Antunez O., Ruiz-Garcia A.B., Perez-Ortin J.E., Tordera V.
FEBS Lett. 579:4063-4068(2005) [PubMed: 16023114] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

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Cross-references

Sequence databases

U18795 Genomic DNA. Translation: AAB65031.1.
PIRS50533.
RefSeqNP_010858.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:2363N.
IntActP39984. 9 interactions.

Proteomic databases

PeptideAtlasP39984.
PRIDEP39984.

Genome annotation databases

EnsemblYEL056W. Saccharomyces cerevisiae. [Contig view]
GeneID856654.
GenomeReviewsGene locus YEL056W in contig U00092_GR.
KEGGsce:YEL056W.
NMPDRfig|4932.3.peg.1907.

Organism-specific databases

CYGDYEL056w.
SGDS000000782. HAT2.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMP39984.
OMAP39984. SNIRITA.

Enzyme and pathway databases

BRENDA2.3.1.48. 250.

Gene expression databases

ArrayExpressP39984.
GermOnlineYEL056W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR015943. WD40/YVTN_repeat-like.
IPR001680. WD40_repeat.
IPR019782. WD40_repeat_2.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_region.
IPR019781. WD40_repeat_sg.
[Graphical view]
Gene3DG3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
PfamPF00400. WD40. 3 hits.
[Graphical view]
SMARTSM00320. WD40. 6 hits.
[Graphical view]
PROSITEPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio982637.

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Entry information

Entry nameHAT2_YEAST
AccessionPrimary (citable) accession number: P39984
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 16, 2009
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents