P39984 (HAT2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histone acetyltransferase type B subunit 2

Gene names

Name:	HAT2
Ordered Locus Names:	YEL056W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulatory subunit of the histone acetylase B (HAT-B) complex. The complex acetylates 'Lys-12' of histone H4 which is required for telomeric silencing. HAT2 is required for high affinity binding of the acetyltransferase to histone H4, for the nuclear location of HAT1 and for the HAT1-HIF1 interaction. Alone, it is unable to bind to H4, requiring HAT1 for high affinity interaction with the histone tail. HAT2 has also a HAT1 independent function in life-span regulation. Ref.6 Ref.7 Ref.8 Ref.9
Subunit structure	Component of the HAT-B complex composed of at least HAT1 and HAT2. In the cytoplasm, this complex binds to the histone H4 tail. In the nucleus, the HAT-B complex has an additional component, the histone H3/H4 chaperone HIF1. Ref.7 Ref.8
Subcellular location	Cytoplasm. Nucleus. Note: The nuclear location requires the presence of HAT2. Ref.7 Ref.8
Induction	Repressed in presence of farnesol, probably through a intracellular decrease of diacylglycerol. Ref.5
Sequence similarities	Belongs to the WD repeat RBAP46/RBAP48/MSI1 family. Contains 6 WD repeats.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Cytoplasm Nucleus
Domain	Repeat WD repeat
Molecular function	Chromatin regulator
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW chromatin assembly or disassembly Inferred from direct assay Ref.1. Source: SGD chromatin silencing at telomere Inferred from genetic interaction Ref.6. Source: SGD histone acetylation Inferred from direct assay Ref.1. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay Ref.1Ref.4. Source: SGD histone acetyltransferase complex Inferred from direct assay Ref.1Ref.4. Source: SGD
Molecular_function	histone binding Inferred from direct assay Ref.1. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
HAT1	Q12341	9	EBI-8185,EBI-8176
HIF1	Q12373	5	EBI-8185,EBI-31911

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 401	401	Histone acetyltransferase type B subunit 2		PRO_0000051014
Regions
	Repeat	116 – 147	32	WD 1
	Repeat	158 – 189	32	WD 2
	Repeat	206 – 237	32	WD 3
	Repeat	249 – 280	32	WD 4
	Repeat	293 – 324	32	WD 5
	Repeat	350 – 381	32	WD 6

Sequences

	Sequence		Length	Mass (Da)
	P39984 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: C27A91C99D9FCAB7	FASTA	401	45,060
	10 20 30 40 50 60 MENQEKPLSV DEEYDLWKSN VPLMYDFVSE TRLTWPSLTV QWLPTPVQEL DGGFIKQELI 70 80 90 100 110 120 IGTHTSGEEE NYLKFAEINL PKEILSNEDP QEEAGEEYQS SLPAPRSNIR ITAKYEHEEE 130 140 150 160 170 180 ITRARYMPQD PNIVATINGQ GTVFLYSRSE GLQSTLKFHK DNGYALSFST LVKGRLLSGS 190 200 210 220 230 240 DDHTVALWEV GSGGDPTKPV RTWNDLHSDI INDNKWHNFN KDLFGTVSED SLLKINDVRA 250 260 270 280 290 300 NNTTIDTVKC PQPFNTLAFS HHSSNLLAAA GMDSYVYLYD LRNMKEPLHH MSGHEDAVNN 310 320 330 340 350 360 LEFSTHVDGV VVSSGSDNRL MMWDLKQIGA EQTPDDAEDG VPELIMVHAG HRSSVNDFDL 370 380 390 400 NPQIPWLVAS AEEENILQVW KCSHSLPIVG GPPKVNKDII S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The major cytoplasmic histone acetyltransferase in yeast: links to chromatin replication and histone metabolism." Parthun M.R., Widom J., Gottschling D.E. Cell 87:85-94(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 83-105, ACETYLATION OF HISTONE H4, INTERACTION WITH HAT1 AND HISTONE H4.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"HAT1 and HAT2 proteins are components of a yeast nuclear histone acetyltransferase enzyme specific for free histone H4." Ruiz-Garcia A.B., Sendra R., Galiana M., Pamblanco M., Perez-Ortin J.E., Tordera V. J. Biol. Chem. 273:12599-12605(1998) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION OF HISTONE H4 BY THE HAT-B COMPLEX.
[5]	"Farnesol-induced growth inhibition in Saccharomyces cerevisiae by a cell cycle mechanism." Machida K., Tanaka T., Yano Y., Otani S., Taniguchi M. Microbiology 145:293-299(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[6]	"Type B histone acetyltransferase Hat1p participates in telomeric silencing." Kelly T.J., Qin S., Gottschling D.E., Parthun M.R. Mol. Cell. Biol. 20:7051-7058(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, ACETYLATION OF HISTONE H4.
[7]	"Hif1 is a component of yeast histone acetyltransferase B, a complex mainly localized in the nucleus." Poveda A., Pamblanco M., Tafrov S., Tordera V., Sternglanz R., Sendra R. J. Biol. Chem. 279:16033-16043(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE HAT-B COMPLEX, FUNCTION OF THE HAT-B COMPLEX, INTERACTION WITH HISTONE H4, SUBCELLULAR LOCATION.
[8]	"The nuclear Hat1p/Hat2p complex: a molecular link between type B histone acetyltransferases and chromatin assembly." Ai X., Parthun M.R. Mol. Cell 14:195-205(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE HAT-B COMPLEX, MASS SPECTROMETRY, INTERACTION WITH HISTONES H3 AND H4, SUBCELLULAR LOCATION.
[9]	"Yeast HAT1 and HAT2 deletions have different life-span and transcriptome phenotypes." Rosaleny L.E., Antunez O., Ruiz-Garcia A.B., Perez-Ortin J.E., Tordera V. FEBS Lett. 579:4063-4068(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18795 Genomic DNA. Translation: AAB65031.1. BK006939 Genomic DNA. Translation: DAA07598.1.
PIR	S50533.
RefSeq	NP_010858.3. NM_001178871.3.
3D structure databases
ProteinModelPortal	P39984.
SMR	P39984. Positions 10-386.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2363N.
IntAct	P39984. 9 interactions.
MINT	MINT-654148.
STRING	4932.YEL056W.
Proteomic databases
PaxDb	P39984.
PeptideAtlas	P39984.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YEL056W; YEL056W; YEL056W.
GeneID	856654.
KEGG	sce:YEL053C. sce:YEL056W.
Organism-specific databases
CYGD	YEL056w.
SGD	S000000782. HAT2.
Phylogenomic databases
eggNOG	COG2319.
GeneTree	ENSGT00570000079069.
HOGENOM	HOG000160330.
KO	K10752.
OMA	VLYDLVI.
OrthoDB	EOG412QFD.
Gene expression databases
Genevestigator	P39984.
GermOnline	YEL056W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.130.10.10. 1 hit.
InterPro	IPR022052. Histone-bd_RBBP4. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Pfam	PF12265. CAF1C_H4-bd. 1 hit. PF00400. WD40. 2 hits. [Graphical view]
SMART	SM00320. WD40. 6 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. 2 hits. PS50082. WD_REPEATS_2. 2 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982637.

Entry information

Entry name

HAT2_YEAST

Accession

Primary (citable) accession number: P39984
Secondary accession number(s): D3DLJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 131 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program