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P39984

- HAT2_YEAST

UniProt

P39984 - HAT2_YEAST

Protein

Histone acetyltransferase type B subunit 2

Gene

HAT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 142 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Regulatory subunit of the histone acetylase B (HAT-B) complex. The complex acetylates 'Lys-12' of histone H4 which is required for telomeric silencing. HAT2 is required for high affinity binding of the acetyltransferase to histone H4, for the nuclear location of HAT1 and for the HAT1-HIF1 interaction. Alone, it is unable to bind to H4, requiring HAT1 for high affinity interaction with the histone tail. HAT2 has also a HAT1 independent function in life-span regulation.4 Publications

    GO - Molecular functioni

    1. histone binding Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. chromatin assembly or disassembly Source: SGD
    2. chromatin silencing at telomere Source: SGD
    3. histone acetylation Source: SGD

    Keywords - Molecular functioni

    Chromatin regulator

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30174-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Histone acetyltransferase type B subunit 2
    Gene namesi
    Name:HAT2
    Ordered Locus Names:YEL056W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYEL056w.
    SGDiS000000782. HAT2.

    Subcellular locationi

    Cytoplasm. Nucleus
    Note: The nuclear location requires the presence of HAT2.

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. histone acetyltransferase complex Source: SGD
    3. nucleus Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 401401Histone acetyltransferase type B subunit 2PRO_0000051014Add
    BLAST

    Proteomic databases

    MaxQBiP39984.
    PaxDbiP39984.
    PeptideAtlasiP39984.

    Expressioni

    Inductioni

    Repressed in presence of farnesol, probably through a intracellular decrease of diacylglycerol.1 Publication

    Gene expression databases

    GenevestigatoriP39984.

    Interactioni

    Subunit structurei

    Component of the HAT-B complex composed of at least HAT1 and HAT2. In the cytoplasm, this complex binds to the histone H4 tail. In the nucleus, the HAT-B complex has an additional component, the histone H3/H4 chaperone HIF1.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HAT1Q123415EBI-8185,EBI-8176
    HIF1Q123733EBI-8185,EBI-31911

    Protein-protein interaction databases

    BioGridi36673. 68 interactions.
    DIPiDIP-2363N.
    IntActiP39984. 7 interactions.
    MINTiMINT-654148.
    STRINGi4932.YEL056W.

    Structurei

    3D structure databases

    ProteinModelPortaliP39984.
    SMRiP39984. Positions 10-386.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati116 – 14732WD 1Add
    BLAST
    Repeati158 – 18932WD 2Add
    BLAST
    Repeati206 – 23732WD 3Add
    BLAST
    Repeati249 – 28032WD 4Add
    BLAST
    Repeati293 – 32432WD 5Add
    BLAST
    Repeati350 – 38132WD 6Add
    BLAST

    Sequence similaritiesi

    Contains 6 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    GeneTreeiENSGT00570000079069.
    HOGENOMiHOG000160330.
    KOiK10752.
    OMAiVLYDLVI.
    OrthoDBiEOG7D865N.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR022052. Histone-bd_RBBP4_N.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF12265. CAF1C_H4-bd. 1 hit.
    PF00400. WD40. 2 hits.
    [Graphical view]
    SMARTiSM00320. WD40. 6 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS00678. WD_REPEATS_1. 2 hits.
    PS50082. WD_REPEATS_2. 2 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39984-1 [UniParc]FASTAAdd to Basket

    « Hide

    MENQEKPLSV DEEYDLWKSN VPLMYDFVSE TRLTWPSLTV QWLPTPVQEL    50
    DGGFIKQELI IGTHTSGEEE NYLKFAEINL PKEILSNEDP QEEAGEEYQS 100
    SLPAPRSNIR ITAKYEHEEE ITRARYMPQD PNIVATINGQ GTVFLYSRSE 150
    GLQSTLKFHK DNGYALSFST LVKGRLLSGS DDHTVALWEV GSGGDPTKPV 200
    RTWNDLHSDI INDNKWHNFN KDLFGTVSED SLLKINDVRA NNTTIDTVKC 250
    PQPFNTLAFS HHSSNLLAAA GMDSYVYLYD LRNMKEPLHH MSGHEDAVNN 300
    LEFSTHVDGV VVSSGSDNRL MMWDLKQIGA EQTPDDAEDG VPELIMVHAG 350
    HRSSVNDFDL NPQIPWLVAS AEEENILQVW KCSHSLPIVG GPPKVNKDII 400
    S 401
    Length:401
    Mass (Da):45,060
    Last modified:February 1, 1995 - v1
    Checksum:iC27A91C99D9FCAB7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18795 Genomic DNA. Translation: AAB65031.1.
    BK006939 Genomic DNA. Translation: DAA07598.1.
    PIRiS50533.
    RefSeqiNP_010858.3. NM_001178871.3.

    Genome annotation databases

    EnsemblFungiiYEL056W; YEL056W; YEL056W.
    GeneIDi856654.
    KEGGisce:YEL056W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18795 Genomic DNA. Translation: AAB65031.1 .
    BK006939 Genomic DNA. Translation: DAA07598.1 .
    PIRi S50533.
    RefSeqi NP_010858.3. NM_001178871.3.

    3D structure databases

    ProteinModelPortali P39984.
    SMRi P39984. Positions 10-386.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36673. 68 interactions.
    DIPi DIP-2363N.
    IntActi P39984. 7 interactions.
    MINTi MINT-654148.
    STRINGi 4932.YEL056W.

    Proteomic databases

    MaxQBi P39984.
    PaxDbi P39984.
    PeptideAtlasi P39984.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YEL056W ; YEL056W ; YEL056W .
    GeneIDi 856654.
    KEGGi sce:YEL056W.

    Organism-specific databases

    CYGDi YEL056w.
    SGDi S000000782. HAT2.

    Phylogenomic databases

    eggNOGi COG2319.
    GeneTreei ENSGT00570000079069.
    HOGENOMi HOG000160330.
    KOi K10752.
    OMAi VLYDLVI.
    OrthoDBi EOG7D865N.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30174-MONOMER.

    Miscellaneous databases

    NextBioi 982637.
    PROi P39984.

    Gene expression databases

    Genevestigatori P39984.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR022052. Histone-bd_RBBP4_N.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF12265. CAF1C_H4-bd. 1 hit.
    PF00400. WD40. 2 hits.
    [Graphical view ]
    SMARTi SM00320. WD40. 6 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS00678. WD_REPEATS_1. 2 hits.
    PS50082. WD_REPEATS_2. 2 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The major cytoplasmic histone acetyltransferase in yeast: links to chromatin replication and histone metabolism."
      Parthun M.R., Widom J., Gottschling D.E.
      Cell 87:85-94(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 83-105, ACETYLATION OF HISTONE H4, INTERACTION WITH HAT1 AND HISTONE H4.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "HAT1 and HAT2 proteins are components of a yeast nuclear histone acetyltransferase enzyme specific for free histone H4."
      Ruiz-Garcia A.B., Sendra R., Galiana M., Pamblanco M., Perez-Ortin J.E., Tordera V.
      J. Biol. Chem. 273:12599-12605(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION OF HISTONE H4 BY THE HAT-B COMPLEX.
    5. "Farnesol-induced growth inhibition in Saccharomyces cerevisiae by a cell cycle mechanism."
      Machida K., Tanaka T., Yano Y., Otani S., Taniguchi M.
      Microbiology 145:293-299(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    6. "Type B histone acetyltransferase Hat1p participates in telomeric silencing."
      Kelly T.J., Qin S., Gottschling D.E., Parthun M.R.
      Mol. Cell. Biol. 20:7051-7058(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ACETYLATION OF HISTONE H4.
    7. "Hif1 is a component of yeast histone acetyltransferase B, a complex mainly localized in the nucleus."
      Poveda A., Pamblanco M., Tafrov S., Tordera V., Sternglanz R., Sendra R.
      J. Biol. Chem. 279:16033-16043(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE HAT-B COMPLEX, FUNCTION OF THE HAT-B COMPLEX, INTERACTION WITH HISTONE H4, SUBCELLULAR LOCATION.
    8. "The nuclear Hat1p/Hat2p complex: a molecular link between type B histone acetyltransferases and chromatin assembly."
      Ai X., Parthun M.R.
      Mol. Cell 14:195-205(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE HAT-B COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH HISTONES H3 AND H4, SUBCELLULAR LOCATION.
    9. "Yeast HAT1 and HAT2 deletions have different life-span and transcriptome phenotypes."
      Rosaleny L.E., Antunez O., Ruiz-Garcia A.B., Perez-Ortin J.E., Tordera V.
      FEBS Lett. 579:4063-4068(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiHAT2_YEAST
    AccessioniPrimary (citable) accession number: P39984
    Secondary accession number(s): D3DLJ4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 142 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3