P39982 (HHY1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hypersensitivity to hygromycin-B protein 1

Gene names

Name:	HHY1
Ordered Locus Names:	YEL059W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	102 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in vacuolar trafficking. Ref.3
Subcellular location	Membrane; Multi-pass membrane protein.
Disruption phenotype	Leads to hygromycin B hypersentsitivity, increased vacuolar fragmentation, defective CPY processing, and extreme sensitivities to pH extremes, divalent cations, and multiple drugs. Ref.3

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 102	102	Hypersensitivity to hygromycin-B protein 1		PRO_0000202603
Regions
	Topological domain	1 – 17	17	Extracellular Potential
	Transmembrane	18 – 38	21	Helical; Potential
	Topological domain	39 – 69	31	Cytoplasmic Potential
	Transmembrane	70 – 90	21	Helical; Potential
	Topological domain	91 – 102	12	Extracellular Potential

Sequences

	Sequence		Length	Mass (Da)
	P39982 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: C5EF6ECA78E2DB9A	FASTA	102	11,341
	10 20 30 40 50 60 MSLSFLLFSP FLPPCFSSIS ICLSVLSTVS FFFAFTIPHY VLRCGSVDEW HIHSSAEDFR 70 80 90 100 TQRCVCAVKL SASLLGCLLA CASWSLLLEV SRIKWHVGTA YS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[3]	"Genomic analysis of severe hypersensitivity to hygromycin B reveals linkage to vacuolar defects and new vacuolar gene functions in Saccharomyces cerevisiae." Banuelos M.G., Moreno D.E., Olson D.K., Nguyen Q., Ricarte F., Aguilera-Sandoval C.R., Gharakhanian E. Curr. Genet. 56:121-137(2010) [PubMed: 20043226] [Abstract] Cited for: DISRUPTION PHENOTYPE, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18795 Genomic DNA. Translation: AAB65028.1.
PIR	S50530.
3D structure databases
ProteinModelPortal	P39982.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5050N.
IntAct	P39982. 2 interactions.
MINT	MINT-570183.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YEL059W; YEL059W; YEL059W.
Organism-specific databases
CYGD	YEL059w.
SGD	S000000785. HHY1.
Gene expression databases
ArrayExpress	P39982.
Genevestigator	P39982.
GermOnline	YEL059W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

HHY1_YEAST

Accession

Primary (citable) accession number: P39982

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	September 21, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program