Reviewed, UniProtKB/Swiss-Prot P39981 (AVT2_YEAST)

Names and origin

Protein names

Recommended name:
    Vacuolar amino acid transporter 2

Gene names

Name:	AVT2
Ordered Locus Names:	YEL064C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	480 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probable amino acid transporter of unknown specificity. Ref.3
Subcellular location	Vacuole membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the amino acid/polyamine transporter 2 family.

Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Membrane Vacuole
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	amino acid transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Ref.3 Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell vacuole Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 480	480	Vacuolar amino acid transporter 2		PRO_0000093835
Regions
	Transmembrane	72 – 92	21	Potential
	Transmembrane	95 – 115	21	Potential
	Transmembrane	145 – 165	21	Potential
	Transmembrane	214 – 234	21	Potential
	Transmembrane	263 – 283	21	Potential
	Transmembrane	297 – 317	21	Potential
	Transmembrane	338 – 358	21	Potential
	Transmembrane	394 – 414	21	Potential
	Transmembrane	447 – 467	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	P39981-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 45799CBD3695B78E	FASTA	480	53,324
	10 20 30 40 50 60 MSELGEYSKL ENKELRTEFE LTNFPFPGTT DNDSDDGSQG QNSLNIITPD MDDTLVNDVL 70 80 90 100 110 120 RENDKKSSMR MAFMNLANSI LGAGIITQPF AIKNAGILGG LLSYVALGFI VDWTLRLIVI 130 140 150 160 170 180 NLTLAGKRTY QGTVEHVMGK KGKLLILFTN GLFAFGGCIG YCIIIGDTIP HVLRAIFSQN 190 200 210 220 230 240 DGNVHFWLRR NVIIVMVTTF ISFPLSMKRN IEALSKASFL AVISMIIIVL TVVIRGPMLP 250 260 270 280 290 300 YDWKGHSLKL SDFFMKATIF RSLSVISFAL VCHHNTSFIF FSMRNRSVAK FTRLTHISII 310 320 330 340 350 360 ISVICCALMG YSGFAVFKEK TKGNVLNSFP GTDTAINIAR LCFGFNMLTT FPMEIFVLRD 370 380 390 400 410 420 VVGNLLHECN LIKNYDEHTQ LSGKQHVVIT SSLVFITMGI SLTTCNLGAL FELIGATTAS 430 440 450 460 470 480 TMAYILPPYT NLLLTSKKKS WKERLPFYLC ICFGFMIMII SSTQTIIDAV NGSDGQHCQI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Mapping of transcription start sites in Saccharomyces cerevisiae using 5' SAGE." Zhang Z., Dietrich F.S. Nucleic Acids Res. 33:2838-2851(2005) [PubMed: 15905473] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-94. Strain: ATCC 208353 / W303-1A.
[3]	"A family of yeast proteins mediating bidirectional vacuolar amino acid transport." Russnak R., Konczal D., McIntire S.L. J. Biol. Chem. 276:23849-23857(2001) [PubMed: 11274162] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U18795 Genomic DNA. Translation: AAB65023.1. AY899246 mRNA. Translation: AAX83931.1.
PIR	S50525.
RefSeq	NP_010850.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5166N.
IntAct	P39981. 6 interactions.
STRING	P39981.
Genome annotation databases
Ensembl	YEL064C; YEL064C; YEL064C; Saccharomyces cerevisiae. [Genome view]
GeneID	856645.
KEGG	sce:YEL064C.
NMPDR	fig|4932.3.peg.1898.
Organism-specific databases
CYGD	YEL064c.
SGD	S000000790. AVT2.
Phylogenomic databases
HOGENOM	P39981.
OMA	ANMANSI
OrthoDB	EOG9PZKQ3
Gene expression databases
ArrayExpress	P39981.
Genevestigator	P39981.
GermOnline	YEL064C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013057. AA_transpt_TM. [Graphical view]
Pfam	PF01490. Aa_trans. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	982613.

Entry information

Entry name

AVT2_YEAST

Accession

Primary (citable) accession number: P39981
Secondary accession number(s): Q2VQX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 24, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)