ID   SIT1_YEAST              Reviewed;         628 AA.
AC   P39980; D3DLI5; E9P951;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   27-MAR-2024, entry version 160.
DE   RecName: Full=Siderophore iron transporter 1;
DE   AltName: Full=Ferrioxamine B permease;
DE   AltName: Full=Siderophore iron transporter ARN3;
GN   Name=SIT1; Synonyms=ARN3; OrderedLocusNames=YEL065W;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SUBCELLULAR LOCATION.
RX   PubMed=10744769; DOI=10.1074/jbc.275.14.10709;
RA   Yun C.-W., Ferea T., Rashford J., Ardon O., Brown P.O., Botstein D.,
RA   Kaplan J., Philpott C.C.;
RT   "Desferrioxamine-mediated iron uptake in Saccharomyces cerevisiae. Evidence
RT   for two pathways of iron uptake.";
RL   J. Biol. Chem. 275:10709-10715(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169868;
RA   Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA   Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA   Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA   Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA   Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA   Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA   Botstein D., Davis R.W.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL   Nature 387:78-81(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [5]
RP   FUNCTION.
RX   PubMed=9884238; DOI=10.1099/00221287-144-12-3455;
RA   Lesuisse E., Simon-Casteras M., Labbe P.;
RT   "Siderophore-mediated iron uptake in Saccharomyces cerevisiae: the SIT1
RT   gene encodes a ferrioxamine B permease that belongs to the major
RT   facilitator superfamily.";
RL   Microbiology 144:3455-3462(1998).
RN   [6]
RP   SUBCELLULAR LOCATION.
RX   PubMed=10748025; DOI=10.1074/jbc.m001456200;
RA   Yun C.-W., Tiedeman J.S., Moore R.E., Philpott C.C.;
RT   "Siderophore-iron uptake in Saccharomyces cerevisiae. Identification of
RT   ferrichrome and fusarinine transporters.";
RL   J. Biol. Chem. 275:16354-16359(2000).
CC   -!- FUNCTION: Involved in the transport of siderophore ferrioxamine B and
CC       so has a role in iron homeostasis. {ECO:0000269|PubMed:9884238}.
CC   -!- SUBCELLULAR LOCATION: Endosome membrane {ECO:0000269|PubMed:10744769,
CC       ECO:0000269|PubMed:10748025}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:10744769, ECO:0000269|PubMed:10748025}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       {ECO:0000305}.
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DR   EMBL; U18795; AAB65022.1; -; Genomic_DNA.
DR   EMBL; AY723800; AAU09717.1; -; Genomic_DNA.
DR   EMBL; BK006939; DAA07589.1; -; Genomic_DNA.
DR   PIR; S50524; S50524.
DR   RefSeq; NP_010849.3; NM_001178880.3.
DR   AlphaFoldDB; P39980; -.
DR   SMR; P39980; -.
DR   BioGRID; 36664; 77.
DR   DIP; DIP-7273N; -.
DR   STRING; 4932.YEL065W; -.
DR   TCDB; 2.A.1.16.1; the major facilitator superfamily (mfs).
DR   iPTMnet; P39980; -.
DR   MaxQB; P39980; -.
DR   PaxDb; 4932-YEL065W; -.
DR   PeptideAtlas; P39980; -.
DR   EnsemblFungi; YEL065W_mRNA; YEL065W; YEL065W.
DR   GeneID; 856644; -.
DR   KEGG; sce:YEL065W; -.
DR   AGR; SGD:S000000791; -.
DR   SGD; S000000791; SIT1.
DR   VEuPathDB; FungiDB:YEL065W; -.
DR   eggNOG; KOG0254; Eukaryota.
DR   GeneTree; ENSGT00940000176305; -.
DR   HOGENOM; CLU_012970_2_1_1; -.
DR   InParanoid; P39980; -.
DR   OMA; VMLNTAW; -.
DR   OrthoDB; 2165012at2759; -.
DR   BioCyc; YEAST:G3O-30180-MONOMER; -.
DR   BioGRID-ORCS; 856644; 0 hits in 10 CRISPR screens.
DR   PRO; PR:P39980; -.
DR   Proteomes; UP000002311; Chromosome V.
DR   RNAct; P39980; Protein.
DR   GO; GO:0031410; C:cytoplasmic vesicle; IDA:SGD.
DR   GO; GO:0005768; C:endosome; IDA:SGD.
DR   GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR   GO; GO:0005774; C:vacuolar membrane; IBA:GO_Central.
DR   GO; GO:0015344; F:siderophore uptake transmembrane transporter activity; IMP:SGD.
DR   GO; GO:0015343; F:siderophore-iron transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0006879; P:intracellular iron ion homeostasis; IDA:SGD.
DR   GO; GO:0015891; P:siderophore transport; IMP:SGD.
DR   GO; GO:0055085; P:transmembrane transport; IMP:SGD.
DR   CDD; cd17322; MFS_ARN_like; 1.
DR   Gene3D; 1.20.1250.20; MFS general substrate transporter like domains; 2.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   PANTHER; PTHR23501:SF92; GLUTATHIONE EXCHANGER 1-RELATED; 1.
DR   PANTHER; PTHR23501; MAJOR FACILITATOR SUPERFAMILY; 1.
DR   SUPFAM; SSF103473; MFS general substrate transporter; 1.
PE   3: Inferred from homology;
KW   Endosome; Ion transport; Iron; Iron transport; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..628
FT                   /note="Siderophore iron transporter 1"
FT                   /id="PRO_0000084878"
FT   TRANSMEM        68..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        107..127
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        132..152
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        164..184
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        194..214
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        225..245
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        285..305
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        317..337
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        354..374
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        394..414
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        420..440
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        448..468
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        488..508
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        559..579
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        386
FT                   /note="E -> G (in Ref. 4; AAU09717)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   628 AA;  70562 MW;  39E711CF861CEF05 CRC64;
     MDPGIANHTL PEEFEEVVVP EMLEKEVGAK VDVKPTLTTS SPAPSYIELI DPGVHNIEIY
     AEMYNRPIYR VALFFSLFLI AYAYGLDGNI RYTFQAYATS SYSQHSLLST VNCIKTVIAA
     VGQIFFARLS DIFGRFSIMI VSIIFYSMGT IIESQAVNIT RFAVGGCFYQ LGLTGIILIL
     EVIASDFSNL NWRLLALFIP ALPFIINTWI SGNVTSAIDA NWKWGIGMWA FILPLACIPL
     GICMLHMRYL ARKHAKDRLK PEFEALNKLK WKSFCIDIAF WKLDIIGMLL ITVFFGCVLV
     PFTLAGGLKE EWKTAHIIVP EVIGWVVVLP LYMLWEIKYS RHPLTPWDLI QDRGIFFALL
     IAFFINFNWY MQGDYMYTVL VVAVHESIKS ATRITSLYSF VSVIVGTILG FILIKVRRTK
     PFIIFGISCW IVSFGLLVHY RGDSGAHSGI IGSLCLLGFG AGSFTYVTQA SIQASAKTHA
     RMAVVTSLYL ATYNIGSAFG SSVSGAVWTN ILPKEISKRI SDPTLAAQAY GSPFTFITTY
     TWGTPERIAL VMSYRYVQKI LCIIGLVFCF PLLGCAFMLR NHKLTDSIAL EGNDHLESKN
     TFEIEEKEES FLKNKFFTHF TSSKDRKD
//