Reviewed, UniProtKB/Swiss-Prot P39980 (SIT1_YEAST)

Names and origin

Protein names

Recommended name:
    Siderophore iron transporter 1
Alternative name(s):
    Siderophore iron transporter ARN3
    Ferrioxamine B permease

Gene names

Name:	SIT1
Synonyms:	ARN3
Ordered Locus Names:	YEL065W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	628 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the transport of siderophore ferrioxamine B and so has a role in iron homeostasis. Ref.3
Subcellular location	Endosome membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the major facilitator superfamily.

Ontologies

Keywords
Biological process	Ion transport Iron transport Transport
Cellular component	Endosome Membrane
Domain	Transmembrane
Ligand	Iron
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	siderophore-iron transport Ref.3 Inferred from mutant phenotype. Source: SGD transmembrane transport Ref.3 Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasmic membrane-bounded vesicle Ref.4 Inferred from direct assay. Source: SGD endosome Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW siderophore-iron (ferrioxamine) uptake transmembrane transporter activity Ref.3 Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 628	628	Siderophore iron transporter 1		PRO_0000084878
Regions
	Transmembrane	68 – 88	21	Potential
	Transmembrane	107 – 127	21	Potential
	Transmembrane	132 – 152	21	Potential
	Transmembrane	164 – 184	21	Potential
	Transmembrane	194 – 214	21	Potential
	Transmembrane	225 – 245	21	Potential
	Transmembrane	285 – 305	21	Potential
	Transmembrane	317 – 337	21	Potential
	Transmembrane	354 – 374	21	Potential
	Transmembrane	394 – 414	21	Potential
	Transmembrane	420 – 440	21	Potential
	Transmembrane	448 – 468	21	Potential
	Transmembrane	488 – 508	21	Potential
	Transmembrane	559 – 579	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	P39980-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 39E711CF861CEF05	FASTA	628	70,562
	10 20 30 40 50 60 MDPGIANHTL PEEFEEVVVP EMLEKEVGAK VDVKPTLTTS SPAPSYIELI DPGVHNIEIY 70 80 90 100 110 120 AEMYNRPIYR VALFFSLFLI AYAYGLDGNI RYTFQAYATS SYSQHSLLST VNCIKTVIAA 130 140 150 160 170 180 VGQIFFARLS DIFGRFSIMI VSIIFYSMGT IIESQAVNIT RFAVGGCFYQ LGLTGIILIL 190 200 210 220 230 240 EVIASDFSNL NWRLLALFIP ALPFIINTWI SGNVTSAIDA NWKWGIGMWA FILPLACIPL 250 260 270 280 290 300 GICMLHMRYL ARKHAKDRLK PEFEALNKLK WKSFCIDIAF WKLDIIGMLL ITVFFGCVLV 310 320 330 340 350 360 PFTLAGGLKE EWKTAHIIVP EVIGWVVVLP LYMLWEIKYS RHPLTPWDLI QDRGIFFALL 370 380 390 400 410 420 IAFFINFNWY MQGDYMYTVL VVAVHESIKS ATRITSLYSF VSVIVGTILG FILIKVRRTK 430 440 450 460 470 480 PFIIFGISCW IVSFGLLVHY RGDSGAHSGI IGSLCLLGFG AGSFTYVTQA SIQASAKTHA 490 500 510 520 530 540 RMAVVTSLYL ATYNIGSAFG SSVSGAVWTN ILPKEISKRI SDPTLAAQAY GSPFTFITTY 550 560 570 580 590 600 TWGTPERIAL VMSYRYVQKI LCIIGLVFCF PLLGCAFMLR NHKLTDSIAL EGNDHLESKN 610 620 TFEIEEKEES FLKNKFFTHF TSSKDRKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Desferrioxamine-mediated iron uptake in Saccharomyces cerevisiae. Evidence for two pathways of iron uptake." Yun C.-W., Ferea T., Rashford J., Ardon O., Brown P.O., Botstein D., Kaplan J., Philpott C.C. J. Biol. Chem. 275:10709-10715(2000) [PubMed: 10744769] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Siderophore-mediated iron uptake in Saccharomyces cerevisiae: the SIT1 gene encodes a ferrioxamine B permease that belongs to the major facilitator superfamily." Lesuisse E., Simon-Casteras M., Labbe P. Microbiology 144:3455-3462(1998) [PubMed: 9884238] [Abstract] Cited for: FUNCTION.
[4]	"Siderophore-iron uptake in Saccharomyces cerevisiae. Identification of ferrichrome and fusarinine transporters." Yun C.-W., Tiedeman J.S., Moore R.E., Philpott C.C. J. Biol. Chem. 275:16354-16359(2000) [PubMed: 10748025] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U18795 Genomic DNA. Translation: AAB65022.1.
PIR	S50524.
RefSeq	NP_010849.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:7273N.
IntAct	P39980. 1 interaction.
STRING	P39980.
Protein family/group databases
TCDB	2.A.1.16.1. major facilitator superfamily (MFS).
Genome annotation databases
Ensembl	YEL065W; YEL065W; YEL065W; Saccharomyces cerevisiae. [Genome view]
GeneID	856644.
KEGG	sce:YEL065W.
NMPDR	fig|4932.3.peg.1897.
Organism-specific databases
CYGD	YEL065w.
SGD	S000000791. SIT1.
Phylogenomic databases
HOGENOM	P39980.
OMA	TSAIDAN
OrthoDB	EOG97STWX
Gene expression databases
ArrayExpress	P39980.
Genevestigator	P39980.
GermOnline	YEL065W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011701. MFS_1. IPR016196. MFS_general_subst_transpt. [Graphical view]
Pfam	PF07690. MFS_1. 1 hit. [Graphical view]
PROSITE	PS50850. MFS. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	982610.

Entry information

Entry name

SIT1_YEAST

Accession

Primary (citable) accession number: P39980

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 24, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)