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Protein

D-amino-acid N-acetyltransferase HPA3

Gene

HPA3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

N-acetyltransferase that acetylates histone H4 at 'Lys-8'. Also acetylates polyamines like putrescine, spermidine and spermine (PubMed:23775086). Acts on a wide range of D-amino acids. Catalyzes the N-acetylation through an ordered bi-bi mechanism, in which acetyl-CoA is the first substrate to be bound and CoA is the last product to be liberated (PubMed:15375647). D-amino acids are toxic for the cell and their N-acetylation, preceding removal from cells, plays an important role in detoxification of D-amino acids (PubMed:10600387, PubMed:16362288).4 Publications

Miscellaneous

Present with 1200 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Acetyl-CoA + a D-amino acid = CoA + an N-acetyl-D-amino acid.1 Publication
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine.1 Publication

Kineticsi

  1. KM=34 mM for D-Serine1 Publication
  2. KM=54 mM for D-Alanine1 Publication
  1. Vmax=22 µmol/min/mg enzyme toward D-Serine1 Publication
  2. Vmax=18 µmol/min/mg enzyme toward D-Alanine1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei144Important for catalytic activityBy similarity1

GO - Molecular functioni

  • D-amino-acid N-acetyltransferase activity Source: SGD
  • histone acetyltransferase activity Source: UniProtKB-EC
  • N-acetyltransferase activity Source: SGD

GO - Biological processi

  • cellular detoxification of nitrogen compound Source: SGD
  • D-amino acid metabolic process Source: SGD
  • protein acetylation Source: SGD

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-30181-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
D-amino-acid N-acetyltransferase HPA31 Publication (EC:2.3.1.361 Publication, EC:2.3.1.481 Publication)
Short name:
DNT
Alternative name(s):
Histone and other protein acetyltransferase 31 Publication
Gene namesi
Name:HPA31 Publication
Ordered Locus Names:YEL066WImported
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL066W
SGDiS000000792 HPA3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000746342 – 161D-amino-acid N-acetyltransferase HPA3Add BLAST160

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Post-translational modificationi

Autoacetylates in an intermolecular reaction.1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP39979
PRIDEiP39979

PTM databases

iPTMnetiP39979

Interactioni

Protein-protein interaction databases

BioGridi36663, 49 interactors
DIPiDIP-2063N
IntActiP39979, 1 interactor
STRINGi4932.YEL066W

Structurei

3D structure databases

ProteinModelPortaliP39979
SMRiP39979
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 161N-acetyltransferasePROSITE-ProRule annotationAdd BLAST148

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni98 – 111Acetyl-CoA bindingBy similarityAdd BLAST14

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000015620
HOGENOMiHOG000078521
InParanoidiP39979
KOiK18852
OrthoDBiEOG092C4ZYL

Family and domain databases

InterProiView protein in InterPro
IPR016181 Acyl_CoA_acyltransferase
IPR000182 GNAT_dom
IPR032962 HPA2/HPA3
PANTHERiPTHR10545:SF42 PTHR10545:SF42, 1 hit
PfamiView protein in Pfam
PF00583 Acetyltransf_1, 1 hit
SUPFAMiSSF55729 SSF55729, 1 hit
PROSITEiView protein in PROSITE
PS51186 GNAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39979-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNEEPEKMV NDRIVVKAIE PKDEEAWNKL WKEYQGFQKT VMPPEVATTT
60 70 80 90 100
FARFIDPTVK LWGALAFDTE TGDAIGFAHY LNHLTSWHVE EVVYMNDLYV
110 120 130 140 150
TERARVKGVG RKLIEFVYSR ADELGTPAVY WVTDHYNHRA QLLYTKVAYK
160
TDKVLYKRNG Y
Length:161
Mass (Da):18,814
Last modified:February 17, 2016 - v2
Checksum:i2A8DB00521975BDC
GO

Sequence cautioni

The sequence AAB65021 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence DAA07588 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18795 Genomic DNA Translation: AAB65021.1 Different initiation.
BK006939 Genomic DNA Translation: DAA07588.1 Different initiation.
PIRiS50523
RefSeqiNP_010848.1, NM_001178881.1

Genome annotation databases

EnsemblFungiiYEL066W; YEL066W; YEL066W
GeneIDi856642
KEGGisce:YEL066W

Similar proteinsi

Entry informationi

Entry nameiHPA3_YEAST
AccessioniPrimary (citable) accession number: P39979
Secondary accession number(s): D3DLI4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 17, 2016
Last modified: May 23, 2018
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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