P39979 (HPA3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetyltransferase HPA3
EC=2.3.1.-

Gene names

Name:	HPA3
Ordered Locus Names:	YEL066W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	N-acetyltransferase whose physiological acetyl acceptor substrate is still unknown. In vitro, histone acetylation is very weak. Ref.3
Subcellular location	Cytoplasm. Nucleus Ref.4.
Post-translational modification	Autoacetylates.
Miscellaneous	Present with 1200 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the acetyltransferase family. GNAT subfamily. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Molecular function	Acyltransferase Transferase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	D-amino acid metabolic process Inferred from mutant phenotype PubMed 16362288. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay Ref.4. Source: SGD nucleus Inferred from direct assay Ref.4. Source: SGD
Molecular_function	D-amino-acid N-acetyltransferase activity Inferred from direct assay PubMed 15375647. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 179	179	N-acetyltransferase HPA3		PRO_0000074634
Regions
	Domain	32 – 179	148	N-acetyltransferase
	Region	116 – 129	14	Acetyl-CoA binding By similarity
Sites
	Site	162	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P39979 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 53A18264798045DD	FASTA	179	20,699
	10 20 30 40 50 60 MKKTPDPSPP FASTKNVGMS NEEPEKMVND RIVVKAIEPK DEEAWNKLWK EYQGFQKTVM 70 80 90 100 110 120 PPEVATTTFA RFIDPTVKLW GALAFDTETG DAIGFAHYLN HLTSWHVEEV VYMNDLYVTE 130 140 150 160 170 RARVKGVGRK LIEFVYSRAD ELGTPAVYWV TDHYNHRAQL LYTKVAYKTD KVLYKRNGY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Crystal structure of the histone acetyltransferase Hpa2: a tetrameric member of the Gcn5-related N-acetyltransferase superfamily." Angus-Hill M.L., Dutnall R.N., Tafrov S.T., Sternglanz R., Ramakrishnan V. J. Mol. Biol. 294:1311-1325(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, ACETYLATION SUBSTRATE.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18795 Genomic DNA. Translation: AAB65021.1. BK006939 Genomic DNA. Translation: DAA07588.1.
PIR	S50523.
RefSeq	NP_010848.1. NM_001178881.1.
3D structure databases
ProteinModelPortal	P39979.
SMR	P39979. Positions 30-179.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2063N.
IntAct	P39979. 3 interactions.
MINT	MINT-492186.
STRING	4932.YEL066W.
Proteomic databases
PaxDb	P39979.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YEL066W; YEL066W; YEL066W.
GeneID	856642.
KEGG	sce:YEL066W.
Organism-specific databases
CYGD	YEL066w.
SGD	S000000792. HPA3.
Phylogenomic databases
eggNOG	COG0454.
GeneTree	ENSGT00390000015620.
HOGENOM	HOG000078521.
OMA	GLTHYLF.
OrthoDB	EOG4W6S5F.
Enzyme and pathway databases
BioCyc	YEAST:G3O-30181-MONOMER.
Gene expression databases
Genevestigator	P39979.
GermOnline	YEL066W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.630.30. 1 hit.
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000182. GNAT_dom. [Graphical view]
Pfam	PF00583. Acetyltransf_1. 1 hit. [Graphical view]
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982604.

Entry information

Entry name

HPA3_YEAST

Accession

Primary (citable) accession number: P39979
Secondary accession number(s): D3DLI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 29, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program