Reviewed, UniProtKB/Swiss-Prot P39979 (HPA3_YEAST)

Names and origin

Protein names

Recommended name:
    N-acetyltransferase HPA3
    EC=2.3.1.-

Gene names

Name:	HPA3
Ordered Locus Names:	YEL066W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	N-acetyltransferase whose physiological acetyl acceptor substrate is still unknown. In vitro, histone acetylation is very weak. Ref.2
Subcellular location	Cytoplasm. Nucleus Ref.3.
Post-translational modification	Autoacetylates.
Miscellaneous	Present with 1200 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the acetyltransferase family. GNAT subfamily. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Molecular function	Acyltransferase Transferase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	D-amino acid metabolic process Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Ref.3 Inferred from direct assay. Source: SGD nucleus Ref.3 Inferred from direct assay. Source: SGD
Molecular function	D-amino-acid N-acetyltransferase activity Inferred from direct assay. Source: SGD identical protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-22400,EBI-22400
	P38800	1	EBI-22400,EBI-24597
SRP1	Q02821	1	EBI-22400,EBI-1797
TEM1	P38987	1	EBI-22400,EBI-19113

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 179	179	N-acetyltransferase HPA3		PRO_0000074634
Regions
	Domain	32 – 179	148	N-acetyltransferase
	Region	116 – 129	14	Acetyl-CoA binding By similarity
Sites
	Site	162	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P39979-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 53A18264798045DD	FASTA	179	20,699
	10 20 30 40 50 60 MKKTPDPSPP FASTKNVGMS NEEPEKMVND RIVVKAIEPK DEEAWNKLWK EYQGFQKTVM 70 80 90 100 110 120 PPEVATTTFA RFIDPTVKLW GALAFDTETG DAIGFAHYLN HLTSWHVEEV VYMNDLYVTE 130 140 150 160 170 RARVKGVGRK LIEFVYSRAD ELGTPAVYWV TDHYNHRAQL LYTKVAYKTD KVLYKRNGY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Crystal structure of the histone acetyltransferase Hpa2: a tetrameric member of the Gcn5-related N-acetyltransferase superfamily." Angus-Hill M.L., Dutnall R.N., Tafrov S.T., Sternglanz R., Ramakrishnan V. J. Mol. Biol. 294:1311-1325(1999) [PubMed: 10600387] [Abstract] Cited for: FUNCTION, ACETYLATION SUBSTRATE.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18795 Genomic DNA. Translation: AAB65021.1.
PIR	S50523.
RefSeq	NP_010848.1.
3D structure databases
SMR	P39979. Positions 30-179.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2063N.
IntAct	P39979. 9 interactions.
STRING	P39979.
Genome annotation databases
Ensembl	YEL066W; YEL066W; YEL066W; Saccharomyces cerevisiae. [Genome view]
GeneID	856642.
KEGG	sce:YEL066W.
NMPDR	fig|4932.3.peg.1896.
Organism-specific databases
CYGD	YEL066w.
SGD	S000000792. HPA3.
Phylogenomic databases
eggNOG	fuNOG11647.
HOGENOM	HBG742014.
OMA	LTHETNA.
OrthoDB	EOG9JQ5DR.
PhylomeDB	P39979.
Gene expression databases
ArrayExpress	P39979.
Genevestigator	P39979.
GermOnline	YEL066W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000182. GCN5-rel_AcTrfase. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
Pfam	PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	982604.

Entry information

Entry name

HPA3_YEAST

Accession

Primary (citable) accession number: P39979

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	January 19, 2010
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)