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Protein

D-amino-acid N-acetyltransferase HPA3

Gene

HPA3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

N-acetyltransferase which acts on a wide range of D-amino acids but shows extremely low activity toward histone. Catalyzes the N-acetylation of D-amino acids through an ordered bi-bi mechanism, in which acetyl-CoA is the first substrate to be bound and CoA is the last product to be liberated. D-amino acids are toxic for the cell and their N-acetylation, preceding removal from cells, plays an important role in detoxification of D-amino acids.2 Publications

Catalytic activityi

Acetyl-CoA + a D-amino acid = CoA + an N-acetyl-D-amino acid.1 Publication

Kineticsi

  1. KM=34 mM for D-Serine1 Publication
  2. KM=54 mM for D-Alanine1 Publication

Vmax=22 µmol/min/mg enzyme toward D-Serine1 Publication

Vmax=18 µmol/min/mg enzyme toward D-Alanine1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei162 – 1621Important for catalytic activityBy similarity

GO - Molecular functioni

  1. D-amino-acid N-acetyltransferase activity Source: SGD

GO - Biological processi

  1. cellular detoxification of nitrogen compound Source: SGD
  2. D-amino acid metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-30181-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
D-amino-acid N-acetyltransferase HPA3 (EC:2.3.1.36)
Short name:
DNT
Alternative name(s):
Histone and other protein acetyltransferase 3
Gene namesi
Name:HPA3
Ordered Locus Names:YEL066W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome V

Organism-specific databases

CYGDiYEL066w.
SGDiS000000792. HPA3.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 179179D-amino-acid N-acetyltransferase HPA3PRO_0000074634Add
BLAST

Post-translational modificationi

Autoacetylates.

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP39979.
PaxDbiP39979.

Expressioni

Gene expression databases

GenevestigatoriP39979.

Interactioni

Protein-protein interaction databases

BioGridi36663. 18 interactions.
DIPiDIP-2063N.
IntActiP39979. 3 interactions.
MINTiMINT-492186.
STRINGi4932.YEL066W.

Structurei

3D structure databases

ProteinModelPortaliP39979.
SMRiP39979. Positions 30-179.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 179148N-acetyltransferasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni116 – 12914Acetyl-CoA bindingBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0454.
GeneTreeiENSGT00390000015620.
HOGENOMiHOG000078521.
InParanoidiP39979.
KOiK18852.
OMAiADDYVRY.
OrthoDBiEOG7X3R3Q.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39979-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKTPDPSPP FASTKNVGMS NEEPEKMVND RIVVKAIEPK DEEAWNKLWK
60 70 80 90 100
EYQGFQKTVM PPEVATTTFA RFIDPTVKLW GALAFDTETG DAIGFAHYLN
110 120 130 140 150
HLTSWHVEEV VYMNDLYVTE RARVKGVGRK LIEFVYSRAD ELGTPAVYWV
160 170
TDHYNHRAQL LYTKVAYKTD KVLYKRNGY
Length:179
Mass (Da):20,699
Last modified:February 1, 1995 - v1
Checksum:i53A18264798045DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18795 Genomic DNA. Translation: AAB65021.1.
BK006939 Genomic DNA. Translation: DAA07588.1.
PIRiS50523.
RefSeqiNP_010848.1. NM_001178881.1.

Genome annotation databases

EnsemblFungiiYEL066W; YEL066W; YEL066W.
GeneIDi856642.
KEGGisce:YEL066W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18795 Genomic DNA. Translation: AAB65021.1.
BK006939 Genomic DNA. Translation: DAA07588.1.
PIRiS50523.
RefSeqiNP_010848.1. NM_001178881.1.

3D structure databases

ProteinModelPortaliP39979.
SMRiP39979. Positions 30-179.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36663. 18 interactions.
DIPiDIP-2063N.
IntActiP39979. 3 interactions.
MINTiMINT-492186.
STRINGi4932.YEL066W.

Proteomic databases

MaxQBiP39979.
PaxDbiP39979.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYEL066W; YEL066W; YEL066W.
GeneIDi856642.
KEGGisce:YEL066W.

Organism-specific databases

CYGDiYEL066w.
SGDiS000000792. HPA3.

Phylogenomic databases

eggNOGiCOG0454.
GeneTreeiENSGT00390000015620.
HOGENOMiHOG000078521.
InParanoidiP39979.
KOiK18852.
OMAiADDYVRY.
OrthoDBiEOG7X3R3Q.

Enzyme and pathway databases

BioCyciYEAST:G3O-30181-MONOMER.

Miscellaneous databases

NextBioi982604.

Gene expression databases

GenevestigatoriP39979.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Crystal structure of the histone acetyltransferase Hpa2: a tetrameric member of the Gcn5-related N-acetyltransferase superfamily."
    Angus-Hill M.L., Dutnall R.N., Tafrov S.T., Sternglanz R., Ramakrishnan V.
    J. Mol. Biol. 294:1311-1325(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ACETYLATION SUBSTRATE.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "D-amino acid N-acetyltransferase of Saccharomyces cerevisiae: a close homologue of histone acetyltransferase Hpa2p acting exclusively on free D-amino acids."
    Yow G.Y., Uo T., Yoshimura T., Esaki N.
    Arch. Microbiol. 182:396-403(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
  7. "Physiological role of D-amino acid-N-acetyltransferase of Saccharomyces cerevisiae: detoxification of D-amino acids."
    Yow G.Y., Uo T., Yoshimura T., Esaki N.
    Arch. Microbiol. 185:39-46(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiHPA3_YEAST
AccessioniPrimary (citable) accession number: P39979
Secondary accession number(s): D3DLI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 4, 2015
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.