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P39979

- HPA3_YEAST

UniProt

P39979 - HPA3_YEAST

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Protein
D-amino-acid N-acetyltransferase HPA3
Gene
HPA3, YEL066W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

N-acetyltransferase which acts on a wide range of D-amino acids but shows extremely low activity toward histone. Catalyzes the N-acetylation of D-amino acids through an ordered bi-bi mechanism, in which acetyl-CoA is the first substrate to be bound and CoA is the last product to be liberated. D-amino acids are toxic for the cell and their N-acetylation, preceding removal from cells, plays an important role in detoxification of D-amino acids.2 Publications

Catalytic activityi

Acetyl-CoA + a D-amino acid = CoA + an N-acetyl-D-amino acid.1 Publication

Kineticsi

  1. KM=34 mM for D-Serine1 Publication
  2. KM=54 mM for D-Alanine

Vmax=22 µmol/min/mg enzyme toward D-Serine

Vmax=18 µmol/min/mg enzyme toward D-Alanine

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei162 – 1621Important for catalytic activity By similarity

GO - Molecular functioni

  1. D-amino-acid N-acetyltransferase activity Source: SGD

GO - Biological processi

  1. D-amino acid metabolic process Source: SGD
  2. cellular detoxification of nitrogen compound Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-30181-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
D-amino-acid N-acetyltransferase HPA3 (EC:2.3.1.36)
Short name:
DNT
Alternative name(s):
Histone and other protein acetyltransferase 3
Gene namesi
Name:HPA3
Ordered Locus Names:YEL066W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYEL066w.
SGDiS000000792. HPA3.

Subcellular locationi

Cytoplasm. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 179179D-amino-acid N-acetyltransferase HPA3
PRO_0000074634Add
BLAST

Post-translational modificationi

Autoacetylates.1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP39979.
PaxDbiP39979.

Expressioni

Gene expression databases

GenevestigatoriP39979.

Interactioni

Protein-protein interaction databases

BioGridi36663. 17 interactions.
DIPiDIP-2063N.
IntActiP39979. 3 interactions.
MINTiMINT-492186.
STRINGi4932.YEL066W.

Structurei

3D structure databases

ProteinModelPortaliP39979.
SMRiP39979. Positions 30-179.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 179148N-acetyltransferase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni116 – 12914Acetyl-CoA binding By similarity
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0454.
GeneTreeiENSGT00390000015620.
HOGENOMiHOG000078521.
OMAiVHFLYHR.
OrthoDBiEOG7X3R3Q.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39979-1 [UniParc]FASTAAdd to Basket

« Hide

MKKTPDPSPP FASTKNVGMS NEEPEKMVND RIVVKAIEPK DEEAWNKLWK    50
EYQGFQKTVM PPEVATTTFA RFIDPTVKLW GALAFDTETG DAIGFAHYLN 100
HLTSWHVEEV VYMNDLYVTE RARVKGVGRK LIEFVYSRAD ELGTPAVYWV 150
TDHYNHRAQL LYTKVAYKTD KVLYKRNGY 179
Length:179
Mass (Da):20,699
Last modified:February 1, 1995 - v1
Checksum:i53A18264798045DD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18795 Genomic DNA. Translation: AAB65021.1.
BK006939 Genomic DNA. Translation: DAA07588.1.
PIRiS50523.
RefSeqiNP_010848.1. NM_001178881.1.

Genome annotation databases

EnsemblFungiiYEL066W; YEL066W; YEL066W.
GeneIDi856642.
KEGGisce:YEL066W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18795 Genomic DNA. Translation: AAB65021.1 .
BK006939 Genomic DNA. Translation: DAA07588.1 .
PIRi S50523.
RefSeqi NP_010848.1. NM_001178881.1.

3D structure databases

ProteinModelPortali P39979.
SMRi P39979. Positions 30-179.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36663. 17 interactions.
DIPi DIP-2063N.
IntActi P39979. 3 interactions.
MINTi MINT-492186.
STRINGi 4932.YEL066W.

Proteomic databases

MaxQBi P39979.
PaxDbi P39979.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YEL066W ; YEL066W ; YEL066W .
GeneIDi 856642.
KEGGi sce:YEL066W.

Organism-specific databases

CYGDi YEL066w.
SGDi S000000792. HPA3.

Phylogenomic databases

eggNOGi COG0454.
GeneTreei ENSGT00390000015620.
HOGENOMi HOG000078521.
OMAi VHFLYHR.
OrthoDBi EOG7X3R3Q.

Enzyme and pathway databases

BioCyci YEAST:G3O-30181-MONOMER.

Miscellaneous databases

NextBioi 982604.

Gene expression databases

Genevestigatori P39979.

Family and domain databases

Gene3Di 3.40.630.30. 1 hit.
InterProi IPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view ]
Pfami PF00583. Acetyltransf_1. 1 hit.
[Graphical view ]
SUPFAMi SSF55729. SSF55729. 1 hit.
PROSITEi PS51186. GNAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Crystal structure of the histone acetyltransferase Hpa2: a tetrameric member of the Gcn5-related N-acetyltransferase superfamily."
    Angus-Hill M.L., Dutnall R.N., Tafrov S.T., Sternglanz R., Ramakrishnan V.
    J. Mol. Biol. 294:1311-1325(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ACETYLATION SUBSTRATE.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "D-amino acid N-acetyltransferase of Saccharomyces cerevisiae: a close homologue of histone acetyltransferase Hpa2p acting exclusively on free D-amino acids."
    Yow G.Y., Uo T., Yoshimura T., Esaki N.
    Arch. Microbiol. 182:396-403(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
  7. "Physiological role of D-amino acid-N-acetyltransferase of Saccharomyces cerevisiae: detoxification of D-amino acids."
    Yow G.Y., Uo T., Yoshimura T., Esaki N.
    Arch. Microbiol. 185:39-46(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHPA3_YEAST
AccessioniPrimary (citable) accession number: P39979
Secondary accession number(s): D3DLI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 14, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1200 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

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