P39976 (DLD3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-lactate dehydrogenase [cytochrome] 3
EC=1.1.2.4

Alternative name(s):
D-lactate ferricytochrome C oxidoreductase
Short name=D-LCR

Gene names

Name:	DLD3
Ordered Locus Names:	YEL071W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	(R)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c + 2 H+.
Cofactor	FAD Potential.
Subcellular location	Cytoplasm Ref.3.
Miscellaneous	Present with 13000 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the FAD-binding oxidoreductase/transferase type 4 family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	filamentous growth Inferred from mutant phenotype PubMed 16999827. Source: SGD lactate metabolic process Inferred from sequence or structural similarity Ref.3. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 11914276. Source: SGD
Molecular_function	D-lactate dehydrogenase (cytochrome) activity Inferred from direct assay Ref.3. Source: SGD UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: InterPro flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 496	496	D-lactate dehydrogenase [cytochrome] 3		PRO_0000128176
Regions
	Domain	64 – 243	180	FAD-binding PCMH-type
Amino acid modifications
	Modified residue	470	1	Phosphoserine Ref.6
	Cross-link	17		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P39976 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 4809F74EDF07F520	FASTA	496	55,225
	10 20 30 40 50 60 MTAAHPVAQL TAEAYPKVKR NPNFKVLDSE DLAYFRSILS NDEILNSQAP EELASFNQDW 70 80 90 100 110 120 MKKYRGQSNL ILLPNSTDKV SKIMKYCNDK KLAVVPQGGN TDLVGASVPV FDEIVLSLRN 130 140 150 160 170 180 MNKVRDFDPV SGTFKCDAGV VMRDAHQFLH DHDHIFPLDL PSRNNCQVGG VVSTNAGGLN 190 200 210 220 230 240 FLRYGSLHGN VLGLEVVLPN GEIISNINAL RKDNTGYDLK QLFIGAEGTI GVVTGVSIVA 250 260 270 280 290 300 AAKPKALNAV FFGIENFDTV QKLFVKAKSE LSEILSAFEF MDRGSIECTI EYLKDLPFPL 310 320 330 340 350 360 ENQHNFYVLI ETSGSNKRHD DEKLTAFLKD TTDSKLISEG MMAKDKADFD RLWTWRKSVP 370 380 390 400 410 420 TACNSYGGMY KYDMSLQLKD LYSVSAAVTE RLNAAGLIGD APKPVVKSCG YGHVGDGNIH 430 440 450 460 470 480 LNIAVREFTK QIEDLLEPFV YEYIASKKGS ISAEHGIGFH KKGKLHYTRS DIEIRFMKDI 490 KNHYDPNGIL NPYKYI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Signalling between mitochondria and the nucleus regulates the expression of a new D-lactate dehydrogenase activity in yeast." Chelstowska A., Liu Z., Jia Y., Amberg D., Butow R.A. Yeast 15:1377-1391(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-17, MASS SPECTROMETRY. Strain: SUB592.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-470, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18795 Genomic DNA. Translation: AAB65016.1. BK006939 Genomic DNA. Translation: DAA07584.1.
PIR	S50518.
RefSeq	NP_010843.1. NM_001178886.1.
3D structure databases
ProteinModelPortal	P39976.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6418N.
IntAct	P39976. 5 interactions.
MINT	MINT-697129.
STRING	4932.YEL071W.
Proteomic databases
PaxDb	P39976.
PeptideAtlas	P39976.
PRIDE	P39976.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YEL071W; YEL071W; YEL071W.
GeneID	856638.
KEGG	sce:YEL071W.
Organism-specific databases
CYGD	YEL071w.
SGD	S000000797. DLD3.
Phylogenomic databases
eggNOG	COG0277.
GeneTree	ENSGT00550000075086.
HOGENOM	HOG000230997.
OMA	RPACVAR.
OrthoDB	EOG4F4WKV.
Gene expression databases
Genevestigator	P39976.
GermOnline	YEL071W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.45.10. 1 hit. 3.30.43.10. 1 hit. 3.30.465.10. 1 hit.
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR016164. FAD-linked_Oxase-like_C. IPR004113. FAD-linked_oxidase_C. IPR006094. Oxid_FAD_bind_N. IPR016171. Vanillyl_alc_oxidase_C-sub2. [Graphical view]
Pfam	PF02913. FAD-oxidase_C. 1 hit. PF01565. FAD_binding_4. 1 hit. [Graphical view]
SUPFAM	SSF55103. FAD-binding_2. 1 hit. SSF56176. FAD-binding_2. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982595.

Entry information

Entry name

DLD3_YEAST

Accession

Primary (citable) accession number: P39976
Secondary accession number(s): D3DLI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program