Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

D-lactate dehydrogenase [cytochrome] 3

Gene

DLD3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(R)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c + 2 H+.

Cofactori

FADCurated

GO - Molecular functioni

  1. D-lactate dehydrogenase (cytochrome) activity Source: SGD
  2. flavin adenine dinucleotide binding Source: InterPro
  3. UDP-N-acetylmuramate dehydrogenase activity Source: InterPro

GO - Biological processi

  1. filamentous growth Source: SGD
  2. lactate metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciYEAST:YEL071W-MONOMER.
ReactomeiREACT_189232. Interconversion of 2-oxoglutarate and 2-hydroxyglutarate.

Names & Taxonomyi

Protein namesi
Recommended name:
D-lactate dehydrogenase [cytochrome] 3 (EC:1.1.2.4)
Alternative name(s):
D-lactate ferricytochrome C oxidoreductase
Short name:
D-LCR
Gene namesi
Name:DLD3
Ordered Locus Names:YEL071W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYEL071w.
SGDiS000000797. DLD3.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 496496D-lactate dehydrogenase [cytochrome] 3PRO_0000128176Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki17 – 17Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP39976.
PaxDbiP39976.
PeptideAtlasiP39976.
PRIDEiP39976.

Expressioni

Gene expression databases

GenevestigatoriP39976.

Interactioni

Protein-protein interaction databases

BioGridi36660. 17 interactions.
DIPiDIP-6418N.
IntActiP39976. 3 interactions.
MINTiMINT-697129.
STRINGi4932.YEL071W.

Structurei

3D structure databases

ProteinModelPortaliP39976.
SMRiP39976. Positions 35-490.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini64 – 243180FAD-binding PCMH-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 FAD-binding PCMH-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0277.
GeneTreeiENSGT00550000075086.
HOGENOMiHOG000230997.
InParanoidiP39976.
OMAiMAHAENG.
OrthoDBiEOG7SJDDF.

Family and domain databases

Gene3Di1.10.45.10. 1 hit.
3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
InterProiIPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR016164. FAD-linked_Oxase-like_C.
IPR004113. FAD-linked_oxidase_C.
IPR006094. Oxid_FAD_bind_N.
IPR016171. Vanillyl_alc_oxidase_C-sub2.
[Graphical view]
PfamiPF02913. FAD-oxidase_C. 1 hit.
PF01565. FAD_binding_4. 1 hit.
[Graphical view]
SUPFAMiSSF55103. SSF55103. 1 hit.
SSF56176. SSF56176. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39976-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAAHPVAQL TAEAYPKVKR NPNFKVLDSE DLAYFRSILS NDEILNSQAP
60 70 80 90 100
EELASFNQDW MKKYRGQSNL ILLPNSTDKV SKIMKYCNDK KLAVVPQGGN
110 120 130 140 150
TDLVGASVPV FDEIVLSLRN MNKVRDFDPV SGTFKCDAGV VMRDAHQFLH
160 170 180 190 200
DHDHIFPLDL PSRNNCQVGG VVSTNAGGLN FLRYGSLHGN VLGLEVVLPN
210 220 230 240 250
GEIISNINAL RKDNTGYDLK QLFIGAEGTI GVVTGVSIVA AAKPKALNAV
260 270 280 290 300
FFGIENFDTV QKLFVKAKSE LSEILSAFEF MDRGSIECTI EYLKDLPFPL
310 320 330 340 350
ENQHNFYVLI ETSGSNKRHD DEKLTAFLKD TTDSKLISEG MMAKDKADFD
360 370 380 390 400
RLWTWRKSVP TACNSYGGMY KYDMSLQLKD LYSVSAAVTE RLNAAGLIGD
410 420 430 440 450
APKPVVKSCG YGHVGDGNIH LNIAVREFTK QIEDLLEPFV YEYIASKKGS
460 470 480 490
ISAEHGIGFH KKGKLHYTRS DIEIRFMKDI KNHYDPNGIL NPYKYI
Length:496
Mass (Da):55,225
Last modified:February 1, 1995 - v1
Checksum:i4809F74EDF07F520
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18795 Genomic DNA. Translation: AAB65016.1.
BK006939 Genomic DNA. Translation: DAA07584.1.
PIRiS50518.
RefSeqiNP_010843.1. NM_001178886.1.

Genome annotation databases

EnsemblFungiiYEL071W; YEL071W; YEL071W.
GeneIDi856638.
KEGGisce:YEL071W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18795 Genomic DNA. Translation: AAB65016.1.
BK006939 Genomic DNA. Translation: DAA07584.1.
PIRiS50518.
RefSeqiNP_010843.1. NM_001178886.1.

3D structure databases

ProteinModelPortaliP39976.
SMRiP39976. Positions 35-490.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36660. 17 interactions.
DIPiDIP-6418N.
IntActiP39976. 3 interactions.
MINTiMINT-697129.
STRINGi4932.YEL071W.

Proteomic databases

MaxQBiP39976.
PaxDbiP39976.
PeptideAtlasiP39976.
PRIDEiP39976.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYEL071W; YEL071W; YEL071W.
GeneIDi856638.
KEGGisce:YEL071W.

Organism-specific databases

CYGDiYEL071w.
SGDiS000000797. DLD3.

Phylogenomic databases

eggNOGiCOG0277.
GeneTreeiENSGT00550000075086.
HOGENOMiHOG000230997.
InParanoidiP39976.
OMAiMAHAENG.
OrthoDBiEOG7SJDDF.

Enzyme and pathway databases

BioCyciYEAST:YEL071W-MONOMER.
ReactomeiREACT_189232. Interconversion of 2-oxoglutarate and 2-hydroxyglutarate.

Miscellaneous databases

NextBioi982595.

Gene expression databases

GenevestigatoriP39976.

Family and domain databases

Gene3Di1.10.45.10. 1 hit.
3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
InterProiIPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR016164. FAD-linked_Oxase-like_C.
IPR004113. FAD-linked_oxidase_C.
IPR006094. Oxid_FAD_bind_N.
IPR016171. Vanillyl_alc_oxidase_C-sub2.
[Graphical view]
PfamiPF02913. FAD-oxidase_C. 1 hit.
PF01565. FAD_binding_4. 1 hit.
[Graphical view]
SUPFAMiSSF55103. SSF55103. 1 hit.
SSF56176. SSF56176. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Signalling between mitochondria and the nucleus regulates the expression of a new D-lactate dehydrogenase activity in yeast."
    Chelstowska A., Liu Z., Jia Y., Amberg D., Butow R.A.
    Yeast 15:1377-1391(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-17.
    Strain: SUB592.
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDLD3_YEAST
AccessioniPrimary (citable) accession number: P39976
Secondary accession number(s): D3DLI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: February 4, 2015
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 13000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.