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P39976 (DLD3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-lactate dehydrogenase [cytochrome] 3

EC=1.1.2.4
Alternative name(s):
D-lactate ferricytochrome C oxidoreductase
Short name=D-LCR
Gene names
Name:DLD3
Ordered Locus Names:YEL071W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length496 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

(R)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c + 2 H+.

Cofactor

FAD Potential.

Subcellular location

Cytoplasm Ref.3.

Miscellaneous

Present with 13000 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the FAD-binding oxidoreductase/transferase type 4 family.

Contains 1 FAD-binding PCMH-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 496496D-lactate dehydrogenase [cytochrome] 3
PRO_0000128176

Regions

Domain64 – 243180FAD-binding PCMH-type

Amino acid modifications

Cross-link17Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.5

Sequences

Sequence LengthMass (Da)Tools
P39976 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 4809F74EDF07F520

FASTA49655,225
        10         20         30         40         50         60 
MTAAHPVAQL TAEAYPKVKR NPNFKVLDSE DLAYFRSILS NDEILNSQAP EELASFNQDW 

        70         80         90        100        110        120 
MKKYRGQSNL ILLPNSTDKV SKIMKYCNDK KLAVVPQGGN TDLVGASVPV FDEIVLSLRN 

       130        140        150        160        170        180 
MNKVRDFDPV SGTFKCDAGV VMRDAHQFLH DHDHIFPLDL PSRNNCQVGG VVSTNAGGLN 

       190        200        210        220        230        240 
FLRYGSLHGN VLGLEVVLPN GEIISNINAL RKDNTGYDLK QLFIGAEGTI GVVTGVSIVA 

       250        260        270        280        290        300 
AAKPKALNAV FFGIENFDTV QKLFVKAKSE LSEILSAFEF MDRGSIECTI EYLKDLPFPL 

       310        320        330        340        350        360 
ENQHNFYVLI ETSGSNKRHD DEKLTAFLKD TTDSKLISEG MMAKDKADFD RLWTWRKSVP 

       370        380        390        400        410        420 
TACNSYGGMY KYDMSLQLKD LYSVSAAVTE RLNAAGLIGD APKPVVKSCG YGHVGDGNIH 

       430        440        450        460        470        480 
LNIAVREFTK QIEDLLEPFV YEYIASKKGS ISAEHGIGFH KKGKLHYTRS DIEIRFMKDI 

       490 
KNHYDPNGIL NPYKYI 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Signalling between mitochondria and the nucleus regulates the expression of a new D-lactate dehydrogenase activity in yeast."
Chelstowska A., Liu Z., Jia Y., Amberg D., Butow R.A.
Yeast 15:1377-1391(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"A proteomics approach to understanding protein ubiquitination."
Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P.
Nat. Biotechnol. 21:921-926(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-17.
Strain: SUB592.
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U18795 Genomic DNA. Translation: AAB65016.1.
BK006939 Genomic DNA. Translation: DAA07584.1.
PIRS50518.
RefSeqNP_010843.1. NM_001178886.1.

3D structure databases

ProteinModelPortalP39976.
SMRP39976. Positions 27-496.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36660. 15 interactions.
DIPDIP-6418N.
IntActP39976. 3 interactions.
MINTMINT-697129.
STRING4932.YEL071W.

Proteomic databases

PaxDbP39976.
PeptideAtlasP39976.
PRIDEP39976.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYEL071W; YEL071W; YEL071W.
GeneID856638.
KEGGsce:YEL071W.

Organism-specific databases

CYGDYEL071w.
SGDS000000797. DLD3.

Phylogenomic databases

eggNOGCOG0277.
GeneTreeENSGT00550000075086.
HOGENOMHOG000230997.
OMAIECTIEY.
OrthoDBEOG7SJDDF.

Enzyme and pathway databases

BioCycYEAST:YEL071W-MONOMER.

Gene expression databases

GenevestigatorP39976.

Family and domain databases

Gene3D1.10.45.10. 1 hit.
3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
InterProIPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR016164. FAD-linked_Oxase-like_C.
IPR004113. FAD-linked_oxidase_C.
IPR006094. Oxid_FAD_bind_N.
IPR016171. Vanillyl_alc_oxidase_C-sub2.
[Graphical view]
PfamPF02913. FAD-oxidase_C. 1 hit.
PF01565. FAD_binding_4. 1 hit.
[Graphical view]
SUPFAMSSF55103. SSF55103. 1 hit.
SSF56176. SSF56176. 1 hit.
PROSITEPS51387. FAD_PCMH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio982595.

Entry information

Entry nameDLD3_YEAST
AccessionPrimary (citable) accession number: P39976
Secondary accession number(s): D3DLI0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 19, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families