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Protein

D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3

Gene

DLD3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of (R)-2-hydroxyglutarate to 2-oxoglutarate coupled to reduction of pyruvate to (R)-lactate. Can also use oxaloacetate as electron acceptor instead of pyruvate producing (R)-malate.1 Publication

Miscellaneous

Present with 13000 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

(R)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c + 2 H+.
(R)-2-hydroxyglutarate + pyruvate = 2-oxoglutarate + (R)-lactate.1 Publication

Cofactori

FADCurated

Kineticsi

kcat is 4.0 sec(-1) for (R)-2-hydroxyglutarate oxidation with pyruvate as electron acceptor. kcat is 6.6 sec(-1) for (R)-2-hydroxyglutarate oxidation with DCIP as electron acceptor. kcat is 4.9 sec(-1) for pyruvate reduction with (R)-2-hydroxyglutarate as electron donor. kcat is 16.5 sec(-1) for (R)-lactate reduction with DCIP as electron donor.1 Publication
  1. KM=111 µM for (R)-2-hydroxyglutarate1 Publication
  2. KM=450 µM for pyruvate1 Publication
  3. KM=533 µM for (R)-lactate1 Publication

    GO - Molecular functioni

    • (D)-2-hydroxyglutarate-pyruvate transhydrogenase activity Source: SGD
    • (R)-2-hydroxyglutarate dehydrogenase activity Source: GO_Central
    • D-lactate dehydrogenase (cytochrome) activity Source: SGD
    • FAD binding Source: SGD

    GO - Biological processi

    • filamentous growth Source: SGD
    • lactate biosynthetic process Source: SGD
    • lactate oxidation Source: GO_Central
    • respiratory electron transport chain Source: GO_Central

    Keywordsi

    Molecular functionOxidoreductase
    LigandFAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciYEAST:YEL071W-MONOMER.
    ReactomeiR-SCE-880009. Interconversion of 2-oxoglutarate and 2-hydroxyglutarate.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3Curated (EC:1.1.99.401 Publication)
    Short name:
    D-2HG--pyruvate transhydrogenase DLD31 Publication
    Alternative name(s):
    (R)-2-hydroxyglutarate--pyruvate transhydrogenaseCurated
    D-lactate dehydrogenase [cytochrome] 3 (EC:1.1.2.4)
    D-lactate ferricytochrome C oxidoreductase
    Short name:
    D-LCR
    Gene namesi
    Name:DLD3
    Ordered Locus Names:YEL071W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome V

    Organism-specific databases

    EuPathDBiFungiDB:YEL071W.
    SGDiS000000797. DLD3.

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001281761 – 496D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3Add BLAST496

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Cross-linki17Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

    Keywords - PTMi

    Isopeptide bond, Ubl conjugation

    Proteomic databases

    MaxQBiP39976.
    PRIDEiP39976.

    PTM databases

    iPTMnetiP39976.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MAD1L1Q9Y6D93EBI-5933,EBI-742610From Homo sapiens.

    Protein-protein interaction databases

    BioGridi36660. 38 interactors.
    DIPiDIP-6418N.
    IntActiP39976. 17 interactors.
    MINTiMINT-697129.
    STRINGi4932.YEL071W.

    Structurei

    3D structure databases

    ProteinModelPortaliP39976.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini64 – 243FAD-binding PCMH-typePROSITE-ProRule annotationAdd BLAST180

    Sequence similaritiesi

    Phylogenomic databases

    GeneTreeiENSGT00550000075086.
    HOGENOMiHOG000230997.
    InParanoidiP39976.
    KOiK21618.
    OrthoDBiEOG092C1XYI.

    Family and domain databases

    Gene3Di1.10.45.10. 1 hit.
    3.30.43.10. 1 hit.
    3.30.465.10. 1 hit.
    InterProiView protein in InterPro
    IPR016169. CO_DH_flavot_FAD-bd_sub2.
    IPR016166. FAD-bd_2.
    IPR016167. FAD-bd_2_sub1.
    IPR016164. FAD-linked_Oxase-like_C.
    IPR004113. FAD-linked_oxidase_C.
    IPR006094. Oxid_FAD_bind_N.
    IPR016171. Vanillyl_alc_oxidase_C-sub2.
    PfamiView protein in Pfam
    PF02913. FAD-oxidase_C. 1 hit.
    PF01565. FAD_binding_4. 1 hit.
    SUPFAMiSSF55103. SSF55103. 1 hit.
    SSF56176. SSF56176. 1 hit.
    PROSITEiView protein in PROSITE
    PS51387. FAD_PCMH. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P39976-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTAAHPVAQL TAEAYPKVKR NPNFKVLDSE DLAYFRSILS NDEILNSQAP
    60 70 80 90 100
    EELASFNQDW MKKYRGQSNL ILLPNSTDKV SKIMKYCNDK KLAVVPQGGN
    110 120 130 140 150
    TDLVGASVPV FDEIVLSLRN MNKVRDFDPV SGTFKCDAGV VMRDAHQFLH
    160 170 180 190 200
    DHDHIFPLDL PSRNNCQVGG VVSTNAGGLN FLRYGSLHGN VLGLEVVLPN
    210 220 230 240 250
    GEIISNINAL RKDNTGYDLK QLFIGAEGTI GVVTGVSIVA AAKPKALNAV
    260 270 280 290 300
    FFGIENFDTV QKLFVKAKSE LSEILSAFEF MDRGSIECTI EYLKDLPFPL
    310 320 330 340 350
    ENQHNFYVLI ETSGSNKRHD DEKLTAFLKD TTDSKLISEG MMAKDKADFD
    360 370 380 390 400
    RLWTWRKSVP TACNSYGGMY KYDMSLQLKD LYSVSAAVTE RLNAAGLIGD
    410 420 430 440 450
    APKPVVKSCG YGHVGDGNIH LNIAVREFTK QIEDLLEPFV YEYIASKKGS
    460 470 480 490
    ISAEHGIGFH KKGKLHYTRS DIEIRFMKDI KNHYDPNGIL NPYKYI
    Length:496
    Mass (Da):55,225
    Last modified:February 1, 1995 - v1
    Checksum:i4809F74EDF07F520
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U18795 Genomic DNA. Translation: AAB65016.1.
    BK006939 Genomic DNA. Translation: DAA07584.1.
    PIRiS50518.
    RefSeqiNP_010843.1. NM_001178886.1.

    Genome annotation databases

    EnsemblFungiiYEL071W; YEL071W; YEL071W.
    GeneIDi856638.
    KEGGisce:YEL071W.

    Similar proteinsi

    Entry informationi

    Entry nameiDLD3_YEAST
    AccessioniPrimary (citable) accession number: P39976
    Secondary accession number(s): D3DLI0
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: September 27, 2017
    This is version 148 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names