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P39976

- DLD3_YEAST

UniProt

P39976 - DLD3_YEAST

Protein

D-lactate dehydrogenase [cytochrome] 3

Gene

DLD3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    (R)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c + 2 H+.

    Cofactori

    FAD.Curated

    GO - Molecular functioni

    1. D-lactate dehydrogenase (cytochrome) activity Source: SGD
    2. flavin adenine dinucleotide binding Source: InterPro
    3. UDP-N-acetylmuramate dehydrogenase activity Source: InterPro

    GO - Biological processi

    1. filamentous growth Source: SGD
    2. lactate metabolic process Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciYEAST:YEL071W-MONOMER.
    ReactomeiREACT_189232. Interconversion of 2-oxoglutarate and 2-hydroxyglutarate.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    D-lactate dehydrogenase [cytochrome] 3 (EC:1.1.2.4)
    Alternative name(s):
    D-lactate ferricytochrome C oxidoreductase
    Short name:
    D-LCR
    Gene namesi
    Name:DLD3
    Ordered Locus Names:YEL071W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYEL071w.
    SGDiS000000797. DLD3.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 496496D-lactate dehydrogenase [cytochrome] 3PRO_0000128176Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki17 – 17Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

    Keywords - PTMi

    Isopeptide bond, Ubl conjugation

    Proteomic databases

    MaxQBiP39976.
    PaxDbiP39976.
    PeptideAtlasiP39976.
    PRIDEiP39976.

    Expressioni

    Gene expression databases

    GenevestigatoriP39976.

    Interactioni

    Protein-protein interaction databases

    BioGridi36660. 15 interactions.
    DIPiDIP-6418N.
    IntActiP39976. 3 interactions.
    MINTiMINT-697129.
    STRINGi4932.YEL071W.

    Structurei

    3D structure databases

    ProteinModelPortaliP39976.
    SMRiP39976. Positions 35-490.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini64 – 243180FAD-binding PCMH-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 FAD-binding PCMH-type domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0277.
    GeneTreeiENSGT00550000075086.
    HOGENOMiHOG000230997.
    OMAiHISYDIG.
    OrthoDBiEOG7SJDDF.

    Family and domain databases

    Gene3Di1.10.45.10. 1 hit.
    3.30.43.10. 1 hit.
    3.30.465.10. 1 hit.
    InterProiIPR016169. CO_DH_flavot_FAD-bd_sub2.
    IPR016166. FAD-bd_2.
    IPR016167. FAD-bd_2_sub1.
    IPR016164. FAD-linked_Oxase-like_C.
    IPR004113. FAD-linked_oxidase_C.
    IPR006094. Oxid_FAD_bind_N.
    IPR016171. Vanillyl_alc_oxidase_C-sub2.
    [Graphical view]
    PfamiPF02913. FAD-oxidase_C. 1 hit.
    PF01565. FAD_binding_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF55103. SSF55103. 1 hit.
    SSF56176. SSF56176. 1 hit.
    PROSITEiPS51387. FAD_PCMH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39976-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTAAHPVAQL TAEAYPKVKR NPNFKVLDSE DLAYFRSILS NDEILNSQAP    50
    EELASFNQDW MKKYRGQSNL ILLPNSTDKV SKIMKYCNDK KLAVVPQGGN 100
    TDLVGASVPV FDEIVLSLRN MNKVRDFDPV SGTFKCDAGV VMRDAHQFLH 150
    DHDHIFPLDL PSRNNCQVGG VVSTNAGGLN FLRYGSLHGN VLGLEVVLPN 200
    GEIISNINAL RKDNTGYDLK QLFIGAEGTI GVVTGVSIVA AAKPKALNAV 250
    FFGIENFDTV QKLFVKAKSE LSEILSAFEF MDRGSIECTI EYLKDLPFPL 300
    ENQHNFYVLI ETSGSNKRHD DEKLTAFLKD TTDSKLISEG MMAKDKADFD 350
    RLWTWRKSVP TACNSYGGMY KYDMSLQLKD LYSVSAAVTE RLNAAGLIGD 400
    APKPVVKSCG YGHVGDGNIH LNIAVREFTK QIEDLLEPFV YEYIASKKGS 450
    ISAEHGIGFH KKGKLHYTRS DIEIRFMKDI KNHYDPNGIL NPYKYI 496
    Length:496
    Mass (Da):55,225
    Last modified:February 1, 1995 - v1
    Checksum:i4809F74EDF07F520
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18795 Genomic DNA. Translation: AAB65016.1.
    BK006939 Genomic DNA. Translation: DAA07584.1.
    PIRiS50518.
    RefSeqiNP_010843.1. NM_001178886.1.

    Genome annotation databases

    EnsemblFungiiYEL071W; YEL071W; YEL071W.
    GeneIDi856638.
    KEGGisce:YEL071W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18795 Genomic DNA. Translation: AAB65016.1 .
    BK006939 Genomic DNA. Translation: DAA07584.1 .
    PIRi S50518.
    RefSeqi NP_010843.1. NM_001178886.1.

    3D structure databases

    ProteinModelPortali P39976.
    SMRi P39976. Positions 35-490.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36660. 15 interactions.
    DIPi DIP-6418N.
    IntActi P39976. 3 interactions.
    MINTi MINT-697129.
    STRINGi 4932.YEL071W.

    Proteomic databases

    MaxQBi P39976.
    PaxDbi P39976.
    PeptideAtlasi P39976.
    PRIDEi P39976.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YEL071W ; YEL071W ; YEL071W .
    GeneIDi 856638.
    KEGGi sce:YEL071W.

    Organism-specific databases

    CYGDi YEL071w.
    SGDi S000000797. DLD3.

    Phylogenomic databases

    eggNOGi COG0277.
    GeneTreei ENSGT00550000075086.
    HOGENOMi HOG000230997.
    OMAi HISYDIG.
    OrthoDBi EOG7SJDDF.

    Enzyme and pathway databases

    BioCyci YEAST:YEL071W-MONOMER.
    Reactomei REACT_189232. Interconversion of 2-oxoglutarate and 2-hydroxyglutarate.

    Miscellaneous databases

    NextBioi 982595.

    Gene expression databases

    Genevestigatori P39976.

    Family and domain databases

    Gene3Di 1.10.45.10. 1 hit.
    3.30.43.10. 1 hit.
    3.30.465.10. 1 hit.
    InterProi IPR016169. CO_DH_flavot_FAD-bd_sub2.
    IPR016166. FAD-bd_2.
    IPR016167. FAD-bd_2_sub1.
    IPR016164. FAD-linked_Oxase-like_C.
    IPR004113. FAD-linked_oxidase_C.
    IPR006094. Oxid_FAD_bind_N.
    IPR016171. Vanillyl_alc_oxidase_C-sub2.
    [Graphical view ]
    Pfami PF02913. FAD-oxidase_C. 1 hit.
    PF01565. FAD_binding_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55103. SSF55103. 1 hit.
    SSF56176. SSF56176. 1 hit.
    PROSITEi PS51387. FAD_PCMH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Signalling between mitochondria and the nucleus regulates the expression of a new D-lactate dehydrogenase activity in yeast."
      Chelstowska A., Liu Z., Jia Y., Amberg D., Butow R.A.
      Yeast 15:1377-1391(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-17.
      Strain: SUB592.
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiDLD3_YEAST
    AccessioniPrimary (citable) accession number: P39976
    Secondary accession number(s): D3DLI0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 13000 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3