Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P39969 (BOI2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein BOI2
Alternative name(s):
Protein BEB1
Gene names
Name:BOI2
Synonyms:BEB1
Ordered Locus Names:YER114C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1040 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds to the BEM1 protein. Involved in bud formation.

Subcellular location

Cytoplasmcytoskeleton.

Miscellaneous

Present with 688 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 PH domain.

Contains 1 SAM (sterile alpha motif) domain.

Contains 1 SH3 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10401040Protein BOI2
PRO_0000064970

Regions

Domain43 – 10765SH3
Domain266 – 33065SAM
Domain768 – 887120PH

Amino acid modifications

Modified residue181Phosphoserine Ref.5 Ref.7 Ref.8
Modified residue241Phosphoserine Ref.8
Modified residue281Phosphoserine Ref.5
Modified residue4501Phosphoserine Ref.5
Modified residue5191Phosphoserine Ref.5 Ref.6 Ref.7 Ref.8
Modified residue5231Phosphoserine Ref.5 Ref.8
Modified residue5461Phosphoserine Ref.7 Ref.8
Modified residue6521Phosphoserine Ref.5

Experimental info

Sequence conflict7331G → A in BAA07427. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P39969 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 2DC635B1A34E7479

FASTA1,040115,688
        10         20         30         40         50         60 
MSNDREVPTL SQLNTTVSRD KDVSDTLSPD FDSKGSATGR DGGNFPMYIA INEYFKRMED 

        70         80         90        100        110        120 
ELDMKPGDKI KVITDDEEYK DGWYFGRNLR TNEEGLYPVV FTQKITVEKA PTLMRAKSTK 

       130        140        150        160        170        180 
RIYSPLTNED PLLSSTFISE NDSNSELPTP QPIETAASIS RTANGKIERN LSLKNTMSDI 

       190        200        210        220        230        240 
DNALLEFKDD SIGPPDRFIN SGRDEEHSIT HETILSATDG LDVVESNSKP TTSSSTGFLN 

       250        260        270        280        290        300 
GDLENQATLI NGIDTTKLNP VEAEFWSPEE ITAYFIMEGY DVQSASRFQK HKISGKILLE 

       310        320        330        340        350        360 
LELVHLKELD INSFGTRFEI FKEIEKIKEA IRTNGRSLNR ASKTNNANIY NQLMPPANVD 

       370        380        390        400        410        420 
QRASYRGHVR KTSQSLEDLP SQQNFIPTPR NTRNSSASKH RPKSLVFDSQ EANANIAPDV 

       430        440        450        460        470        480 
QIPQVVEEMA GNENLFVSPR RAPKPPSYPS PAQPPKSPLL NNTRTSPSPA QLYSWQSPTL 

       490        500        510        520        530        540 
SFSGPKRTSY IDQYSSSDSN FNSRSALPKN NQGGGKALSP IPSPTRNSVR NEDSEGKLTS 

       550        560        570        580        590        600 
SSKRNSVPYY GYAPESSSDR KSSCSSHEEE QFQETMNTFE RPTSSIYADG STIASISNDK 

       610        620        630        640        650        660 
LAHEKEGKKK PTRHSSSLSS KSKSDSRRNS SLKRSSSASR TSSFKKSSFM LSPFRQQFTD 

       670        680        690        700        710        720 
NAARSSSPEE NPITSMPSEK NSSPIVDKKS SKKSRSKRRS VSAKEAEIFT ETVKDDKNKR 

       730        740        750        760        770        780 
SASEAIKGET LKGKSLRQMT ARPVAKKKQT SAFIEGLRSI SVKEAMKDAD FSGWMSKKGS 

       790        800        810        820        830        840 
GAMSTWKTRF FTLHGTRLSY FSSTTDTRER GLIDITAHRV VPAKEDDKLV SLYAASTGKG 

       850        860        870        880        890        900 
RYCFKLLPPQ PGSKKGLTFT QPRTHYFAVD NKEEMRGWMA ALIKTTIDID TSVPIISSYT 

       910        920        930        940        950        960 
TPTVSLSKAQ EMLAEAREET KLREQQMLEN EEDEDQFLWD QQQLQQQQHD NNQGQADRTI 

       970        980        990       1000       1010       1020 
SASTQRTSDE DNTISTPNLS SANNTTIGSN GFSSPFLLAS GLLSPGVARN SSMRGTEKKG 

      1030       1040 
KFSTEEDYFG DNSKHKTDKI 

« Hide

References

« Hide 'large scale' references
[1]"Yeast src homology region 3 domain-binding proteins involved in bud formation."
Matsui Y., Matsui R., Akada R., Toh-e A.
J. Cell Biol. 133:865-878(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18; SER-28; SER-450; SER-519; SER-523 AND SER-652, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[6]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-519, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18; SER-519 AND SER-546, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18; SER-24; SER-519; SER-523 AND SER-546, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D38310 Genomic DNA. Translation: BAA07427.1.
U18916 Genomic DNA. Translation: AAC03212.1.
BK006939 Genomic DNA. Translation: DAA07774.1.
PIRS50617.
RefSeqNP_011039.1. NM_001179004.1.

3D structure databases

ProteinModelPortalP39969.
SMRP39969. Positions 47-106, 263-329, 767-891.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36859. 71 interactions.
DIPDIP-2227N.
IntActP39969. 33 interactions.
MINTMINT-537009.
STRING4932.YER114C.

Proteomic databases

PaxDbP39969.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYER114C; YER114C; YER114C.
GeneID856850.
KEGGsce:YER114C.

Organism-specific databases

CYGDYER114c.
SGDS000000916. BOI2.

Phylogenomic databases

eggNOGNOG73665.
GeneTreeENSGT00530000066577.
HOGENOMHOG000066063.
OMAYCFKLLP.
OrthoDBEOG7N63W3.

Enzyme and pathway databases

BioCycYEAST:G3O-30278-MONOMER.

Gene expression databases

GenevestigatorP39969.

Family and domain databases

Gene3D1.10.150.50. 1 hit.
2.30.29.30. 1 hit.
InterProIPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR011510. SAM_2.
IPR001452. SH3_domain.
[Graphical view]
PfamPF00169. PH. 1 hit.
PF07647. SAM_2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTSM00233. PH. 1 hit.
SM00454. SAM. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF47769. SSF47769. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEPS50003. PH_DOMAIN. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio983183.

Entry information

Entry nameBOI2_YEAST
AccessionPrimary (citable) accession number: P39969
Secondary accession number(s): D3DM20
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families