Protein BOI2 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P39969 (BOI2_YEAST)

Last modified November 3, 2009. Version 91. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Protein BOI2
Alternative name(s):
Protein BEB1

Gene names

Name:	BOI2
Synonyms:	BEB1
Ordered Locus Names:	YER114C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	1040 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Binds to the BEM1 protein. Involved in bud formation.
Subcellular location	Cytoplasm › cytoskeleton.
Miscellaneous	Present with 688 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Contains 1 PH domain. Contains 1 SAM (sterile alpha motif) domain. Contains 1 SH3 domain.

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Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Domain	SH3 domain
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Rho protein signal transduction Traceable author statement. Source: SGD budding cell apical bud growth Inferred from genetic interaction. Source: SGD cytokinesis, completion of separation Inferred from genetic interaction. Source: SGD establishment of cell polarity Traceable author statement. Source: SGD
Cellular component	cellular bud neck Inferred from direct assay. Source: SGD cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
	P40095	1	EBI-3727,EBI-22734
	Q03780	1	EBI-3727,EBI-30094
BBP1	Q12365	1	EBI-3727,EBI-3448

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1040

1040

Protein BOI2

PRO_0000064970

Regions

Domain

43 – 107

SH3

Domain

266 – 330

SAM

Domain

768 – 887

120

Amino acid modifications

Modified residue

Phosphothreonine Ref.8

Modified residue

Phosphoserine Ref.8 Ref.5

Modified residue

Phosphoserine Ref.8 Ref.5 Ref.7

Modified residue

Phosphoserine Ref.7

Modified residue

Phosphoserine Ref.7

Modified residue

118

Phosphoserine Ref.6

Modified residue

178

Phosphoserine Ref.7

Modified residue

191

Phosphoserine Ref.7

Modified residue

372

Phosphothreonine Ref.8 Ref.7

Modified residue

373

Phosphoserine Ref.8

Modified residue

375

Phosphoserine Ref.8 Ref.7

Modified residue

450

Phosphoserine Ref.5

Modified residue

519

Phosphoserine Ref.8 Ref.5 Ref.3

Modified residue

523

Phosphoserine Ref.8 Ref.5

Modified residue

525

Phosphothreonine Ref.3

Modified residue

546

Phosphoserine Ref.8 Ref.7

Modified residue

652

Phosphoserine Ref.5

Modified residue

665

Phosphoserine Ref.8

Modified residue

666

Phosphoserine Ref.8

Modified residue

667

Phosphoserine Ref.8

Modified residue

683

Phosphoserine Ref.8 Ref.7

Modified residue

721

Phosphoserine Ref.7

Modified residue

723

Phosphoserine Ref.8

Experimental info

Sequence conflict

733

G → A in BAA07427. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P39969-1 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 2DC635B1A34E7479
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FASTA

1,040

115,688

        10         20         30         40         50         60 
MSNDREVPTL SQLNTTVSRD KDVSDTLSPD FDSKGSATGR DGGNFPMYIA INEYFKRMED 

        70         80         90        100        110        120 
ELDMKPGDKI KVITDDEEYK DGWYFGRNLR TNEEGLYPVV FTQKITVEKA PTLMRAKSTK 

       130        140        150        160        170        180 
RIYSPLTNED PLLSSTFISE NDSNSELPTP QPIETAASIS RTANGKIERN LSLKNTMSDI 

       190        200        210        220        230        240 
DNALLEFKDD SIGPPDRFIN SGRDEEHSIT HETILSATDG LDVVESNSKP TTSSSTGFLN 

       250        260        270        280        290        300 
GDLENQATLI NGIDTTKLNP VEAEFWSPEE ITAYFIMEGY DVQSASRFQK HKISGKILLE 

       310        320        330        340        350        360 
LELVHLKELD INSFGTRFEI FKEIEKIKEA IRTNGRSLNR ASKTNNANIY NQLMPPANVD 

       370        380        390        400        410        420 
QRASYRGHVR KTSQSLEDLP SQQNFIPTPR NTRNSSASKH RPKSLVFDSQ EANANIAPDV 

       430        440        450        460        470        480 
QIPQVVEEMA GNENLFVSPR RAPKPPSYPS PAQPPKSPLL NNTRTSPSPA QLYSWQSPTL 

       490        500        510        520        530        540 
SFSGPKRTSY IDQYSSSDSN FNSRSALPKN NQGGGKALSP IPSPTRNSVR NEDSEGKLTS 

       550        560        570        580        590        600 
SSKRNSVPYY GYAPESSSDR KSSCSSHEEE QFQETMNTFE RPTSSIYADG STIASISNDK 

       610        620        630        640        650        660 
LAHEKEGKKK PTRHSSSLSS KSKSDSRRNS SLKRSSSASR TSSFKKSSFM LSPFRQQFTD 

       670        680        690        700        710        720 
NAARSSSPEE NPITSMPSEK NSSPIVDKKS SKKSRSKRRS VSAKEAEIFT ETVKDDKNKR 

       730        740        750        760        770        780 
SASEAIKGET LKGKSLRQMT ARPVAKKKQT SAFIEGLRSI SVKEAMKDAD FSGWMSKKGS 

       790        800        810        820        830        840 
GAMSTWKTRF FTLHGTRLSY FSSTTDTRER GLIDITAHRV VPAKEDDKLV SLYAASTGKG 

       850        860        870        880        890        900 
RYCFKLLPPQ PGSKKGLTFT QPRTHYFAVD NKEEMRGWMA ALIKTTIDID TSVPIISSYT 

       910        920        930        940        950        960 
TPTVSLSKAQ EMLAEAREET KLREQQMLEN EEDEDQFLWD QQQLQQQQHD NNQGQADRTI 

       970        980        990       1000       1010       1020 
SASTQRTSDE DNTISTPNLS SANNTTIGSN GFSSPFLLAS GLLSPGVARN SSMRGTEKKG 

      1030       1040 
KFSTEEDYFG DNSKHKTDKI

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast src homology region 3 domain-binding proteins involved in bud formation." Matsui Y., Matsui R., Akada R., Toh-e A. J. Cell Biol. 133:865-878(1996) [PubMed: 8666671] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed: 11875433] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-519 AND THR-525, MASS SPECTROMETRY.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18; SER-28; SER-450; SER-519; SER-523 AND SER-652, MASS SPECTROMETRY.
[6]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-118, MASS SPECTROMETRY.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-28; SER-33; SER-36; SER-178; SER-191; THR-372; SER-375; SER-546; SER-683 AND SER-721, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-15; SER-18; SER-28; THR-372; SER-373; SER-375; SER-519; SER-523; SER-546; SER-665; SER-666; SER-667; SER-683 AND SER-723, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	D38310 Genomic DNA. Translation: BAA07427.1. U18916 Genomic DNA. Translation: AAC03212.1.
PIR	S50617.
RefSeq	NP_011039.1.
3D structure databases
HSSP	HSSP built from PDB template 1FAO based on UniProtKB Q9UHF2.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2227N.
IntAct	P39969. 23 interactions.
STRING	P39969.
Genome annotation databases
Ensembl	YER114C; YER114C; YER114C; Saccharomyces cerevisiae. [Genome view]
GeneID	856850.
GenomeReviews	Gene locus YER114C in contig U00092_GR.
KEGG	sce:YER114C.
NMPDR	fig\|4932.3.peg.2115.
Organism-specific databases
CYGD	YER114c.
SGD	S000000916. BOI2.
Phylogenomic databases
HOGENOM	P39969.
OMA	PKTHYFA.
Gene expression databases
ArrayExpress	P39969.
Genevestigator	P39969.
GermOnline	YER114C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR001660. SAM. IPR011510. SAM_2. IPR001452. SH3_domain. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
Pfam	PF00169. PH. 1 hit. PF07647. SAM_2. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
ProDom	PD000066. SH3. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00233. PH. 1 hit. SM00454. SAM. 1 hit. SM00326. SH3. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. PS50105. SAM_DOMAIN. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	983183.

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Entry information

Entry name

BOI2_YEAST

Accession

Primary (citable) accession number: P39969

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 3, 2009
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents