Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P39969

- BOI2_YEAST

UniProt

P39969 - BOI2_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Protein BOI2

Gene

BOI2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Binds to the BEM1 protein. Involved in bud formation.

GO - Molecular functioni

  1. phospholipid binding Source: SGD

GO - Biological processi

  1. budding cell apical bud growth Source: SGD
  2. cytokinesis, completion of separation Source: SGD
  3. establishment of cell polarity Source: SGD
  4. Rho protein signal transduction Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-30278-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein BOI2
Alternative name(s):
Protein BEB1
Gene namesi
Name:BOI2
Synonyms:BEB1
Ordered Locus Names:YER114C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER114c.
SGDiS000000916. BOI2.

Subcellular locationi

GO - Cellular componenti

  1. cellular bud Source: SGD
  2. cellular bud neck Source: SGD
  3. cytoplasm Source: UniProtKB-KW
  4. cytoskeleton Source: UniProtKB-KW
  5. site of polarized growth Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10401040Protein BOI2PRO_0000064970Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei18 – 181Phosphoserine3 Publications
Modified residuei24 – 241Phosphoserine1 Publication
Modified residuei28 – 281Phosphoserine1 Publication
Modified residuei450 – 4501Phosphoserine1 Publication
Modified residuei519 – 5191Phosphoserine4 Publications
Modified residuei523 – 5231Phosphoserine2 Publications
Modified residuei546 – 5461Phosphoserine2 Publications
Modified residuei652 – 6521Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39969.
PaxDbiP39969.

Expressioni

Gene expression databases

GenevestigatoriP39969.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
BEM1P293666EBI-3727,EBI-3508
DSE1P400773EBI-3727,EBI-22676
FIR1P400202EBI-3727,EBI-13431
YDR239CQ037802EBI-3727,EBI-30094
YER158CP400953EBI-3727,EBI-22734

Protein-protein interaction databases

BioGridi36859. 73 interactions.
DIPiDIP-2227N.
IntActiP39969. 33 interactions.
MINTiMINT-537009.
STRINGi4932.YER114C.

Structurei

3D structure databases

ProteinModelPortaliP39969.
SMRiP39969. Positions 48-104, 262-329.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 10765SH3PROSITE-ProRule annotationAdd
BLAST
Domaini266 – 33065SAMPROSITE-ProRule annotationAdd
BLAST
Domaini768 – 887120PHPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiNOG73665.
GeneTreeiENSGT00530000066577.
HOGENOMiHOG000066063.
InParanoidiP39969.
OMAiSITHETI.
OrthoDBiEOG7N63W3.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR011510. SAM_2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF07647. SAM_2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00454. SAM. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39969-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNDREVPTL SQLNTTVSRD KDVSDTLSPD FDSKGSATGR DGGNFPMYIA
60 70 80 90 100
INEYFKRMED ELDMKPGDKI KVITDDEEYK DGWYFGRNLR TNEEGLYPVV
110 120 130 140 150
FTQKITVEKA PTLMRAKSTK RIYSPLTNED PLLSSTFISE NDSNSELPTP
160 170 180 190 200
QPIETAASIS RTANGKIERN LSLKNTMSDI DNALLEFKDD SIGPPDRFIN
210 220 230 240 250
SGRDEEHSIT HETILSATDG LDVVESNSKP TTSSSTGFLN GDLENQATLI
260 270 280 290 300
NGIDTTKLNP VEAEFWSPEE ITAYFIMEGY DVQSASRFQK HKISGKILLE
310 320 330 340 350
LELVHLKELD INSFGTRFEI FKEIEKIKEA IRTNGRSLNR ASKTNNANIY
360 370 380 390 400
NQLMPPANVD QRASYRGHVR KTSQSLEDLP SQQNFIPTPR NTRNSSASKH
410 420 430 440 450
RPKSLVFDSQ EANANIAPDV QIPQVVEEMA GNENLFVSPR RAPKPPSYPS
460 470 480 490 500
PAQPPKSPLL NNTRTSPSPA QLYSWQSPTL SFSGPKRTSY IDQYSSSDSN
510 520 530 540 550
FNSRSALPKN NQGGGKALSP IPSPTRNSVR NEDSEGKLTS SSKRNSVPYY
560 570 580 590 600
GYAPESSSDR KSSCSSHEEE QFQETMNTFE RPTSSIYADG STIASISNDK
610 620 630 640 650
LAHEKEGKKK PTRHSSSLSS KSKSDSRRNS SLKRSSSASR TSSFKKSSFM
660 670 680 690 700
LSPFRQQFTD NAARSSSPEE NPITSMPSEK NSSPIVDKKS SKKSRSKRRS
710 720 730 740 750
VSAKEAEIFT ETVKDDKNKR SASEAIKGET LKGKSLRQMT ARPVAKKKQT
760 770 780 790 800
SAFIEGLRSI SVKEAMKDAD FSGWMSKKGS GAMSTWKTRF FTLHGTRLSY
810 820 830 840 850
FSSTTDTRER GLIDITAHRV VPAKEDDKLV SLYAASTGKG RYCFKLLPPQ
860 870 880 890 900
PGSKKGLTFT QPRTHYFAVD NKEEMRGWMA ALIKTTIDID TSVPIISSYT
910 920 930 940 950
TPTVSLSKAQ EMLAEAREET KLREQQMLEN EEDEDQFLWD QQQLQQQQHD
960 970 980 990 1000
NNQGQADRTI SASTQRTSDE DNTISTPNLS SANNTTIGSN GFSSPFLLAS
1010 1020 1030 1040
GLLSPGVARN SSMRGTEKKG KFSTEEDYFG DNSKHKTDKI
Length:1,040
Mass (Da):115,688
Last modified:February 1, 1995 - v1
Checksum:i2DC635B1A34E7479
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti733 – 7331G → A in BAA07427. (PubMed:8666671)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38310 Genomic DNA. Translation: BAA07427.1.
U18916 Genomic DNA. Translation: AAC03212.1.
BK006939 Genomic DNA. Translation: DAA07774.1.
PIRiS50617.
RefSeqiNP_011039.1. NM_001179004.1.

Genome annotation databases

EnsemblFungiiYER114C; YER114C; YER114C.
GeneIDi856850.
KEGGisce:YER114C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38310 Genomic DNA. Translation: BAA07427.1 .
U18916 Genomic DNA. Translation: AAC03212.1 .
BK006939 Genomic DNA. Translation: DAA07774.1 .
PIRi S50617.
RefSeqi NP_011039.1. NM_001179004.1.

3D structure databases

ProteinModelPortali P39969.
SMRi P39969. Positions 48-104, 262-329.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36859. 73 interactions.
DIPi DIP-2227N.
IntActi P39969. 33 interactions.
MINTi MINT-537009.
STRINGi 4932.YER114C.

Proteomic databases

MaxQBi P39969.
PaxDbi P39969.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER114C ; YER114C ; YER114C .
GeneIDi 856850.
KEGGi sce:YER114C.

Organism-specific databases

CYGDi YER114c.
SGDi S000000916. BOI2.

Phylogenomic databases

eggNOGi NOG73665.
GeneTreei ENSGT00530000066577.
HOGENOMi HOG000066063.
InParanoidi P39969.
OMAi SITHETI.
OrthoDBi EOG7N63W3.

Enzyme and pathway databases

BioCyci YEAST:G3O-30278-MONOMER.

Miscellaneous databases

NextBioi 983183.

Gene expression databases

Genevestigatori P39969.

Family and domain databases

Gene3Di 1.10.150.50. 1 hit.
2.30.29.30. 1 hit.
InterProi IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR011510. SAM_2.
IPR001452. SH3_domain.
[Graphical view ]
Pfami PF00169. PH. 1 hit.
PF07647. SAM_2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view ]
SMARTi SM00233. PH. 1 hit.
SM00454. SAM. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view ]
SUPFAMi SSF47769. SSF47769. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEi PS50003. PH_DOMAIN. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Yeast src homology region 3 domain-binding proteins involved in bud formation."
    Matsui Y., Matsui R., Akada R., Toh-e A.
    J. Cell Biol. 133:865-878(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18; SER-28; SER-450; SER-519; SER-523 AND SER-652, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  6. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-519, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18; SER-519 AND SER-546, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18; SER-24; SER-519; SER-523 AND SER-546, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBOI2_YEAST
AccessioniPrimary (citable) accession number: P39969
Secondary accession number(s): D3DM20
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 26, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 688 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3