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P39969

- BOI2_YEAST

UniProt

P39969 - BOI2_YEAST

Protein

Protein BOI2

Gene

BOI2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Binds to the BEM1 protein. Involved in bud formation.

    GO - Molecular functioni

    1. phospholipid binding Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. budding cell apical bud growth Source: SGD
    2. cytokinesis, completion of separation Source: SGD
    3. establishment of cell polarity Source: SGD
    4. Rho protein signal transduction Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30278-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein BOI2
    Alternative name(s):
    Protein BEB1
    Gene namesi
    Name:BOI2
    Synonyms:BEB1
    Ordered Locus Names:YER114C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYER114c.
    SGDiS000000916. BOI2.

    Subcellular locationi

    GO - Cellular componenti

    1. cellular bud Source: SGD
    2. cellular bud neck Source: SGD
    3. cytoplasm Source: UniProtKB-KW
    4. cytoskeleton Source: UniProtKB-SubCell
    5. site of polarized growth Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10401040Protein BOI2PRO_0000064970Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei18 – 181Phosphoserine3 Publications
    Modified residuei24 – 241Phosphoserine1 Publication
    Modified residuei28 – 281Phosphoserine1 Publication
    Modified residuei450 – 4501Phosphoserine1 Publication
    Modified residuei519 – 5191Phosphoserine4 Publications
    Modified residuei523 – 5231Phosphoserine2 Publications
    Modified residuei546 – 5461Phosphoserine2 Publications
    Modified residuei652 – 6521Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP39969.
    PaxDbiP39969.

    Expressioni

    Gene expression databases

    GenevestigatoriP39969.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BEM1P293666EBI-3727,EBI-3508
    DSE1P400773EBI-3727,EBI-22676
    FIR1P400202EBI-3727,EBI-13431
    YDR239CQ037802EBI-3727,EBI-30094
    YER158CP400953EBI-3727,EBI-22734

    Protein-protein interaction databases

    BioGridi36859. 71 interactions.
    DIPiDIP-2227N.
    IntActiP39969. 33 interactions.
    MINTiMINT-537009.
    STRINGi4932.YER114C.

    Structurei

    3D structure databases

    ProteinModelPortaliP39969.
    SMRiP39969. Positions 48-105.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini43 – 10765SH3PROSITE-ProRule annotationAdd
    BLAST
    Domaini266 – 33065SAMPROSITE-ProRule annotationAdd
    BLAST
    Domaini768 – 887120PHPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH3 domain

    Phylogenomic databases

    eggNOGiNOG73665.
    GeneTreeiENSGT00530000066577.
    HOGENOMiHOG000066063.
    OMAiSITHETI.
    OrthoDBiEOG7N63W3.

    Family and domain databases

    Gene3Di1.10.150.50. 1 hit.
    2.30.29.30. 1 hit.
    InterProiIPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR001660. SAM.
    IPR013761. SAM/pointed.
    IPR011510. SAM_2.
    IPR001452. SH3_domain.
    [Graphical view]
    PfamiPF00169. PH. 1 hit.
    PF07647. SAM_2. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view]
    SMARTiSM00233. PH. 1 hit.
    SM00454. SAM. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF47769. SSF47769. 1 hit.
    SSF50044. SSF50044. 1 hit.
    PROSITEiPS50003. PH_DOMAIN. 1 hit.
    PS50105. SAM_DOMAIN. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39969-1 [UniParc]FASTAAdd to Basket

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    MSNDREVPTL SQLNTTVSRD KDVSDTLSPD FDSKGSATGR DGGNFPMYIA     50
    INEYFKRMED ELDMKPGDKI KVITDDEEYK DGWYFGRNLR TNEEGLYPVV 100
    FTQKITVEKA PTLMRAKSTK RIYSPLTNED PLLSSTFISE NDSNSELPTP 150
    QPIETAASIS RTANGKIERN LSLKNTMSDI DNALLEFKDD SIGPPDRFIN 200
    SGRDEEHSIT HETILSATDG LDVVESNSKP TTSSSTGFLN GDLENQATLI 250
    NGIDTTKLNP VEAEFWSPEE ITAYFIMEGY DVQSASRFQK HKISGKILLE 300
    LELVHLKELD INSFGTRFEI FKEIEKIKEA IRTNGRSLNR ASKTNNANIY 350
    NQLMPPANVD QRASYRGHVR KTSQSLEDLP SQQNFIPTPR NTRNSSASKH 400
    RPKSLVFDSQ EANANIAPDV QIPQVVEEMA GNENLFVSPR RAPKPPSYPS 450
    PAQPPKSPLL NNTRTSPSPA QLYSWQSPTL SFSGPKRTSY IDQYSSSDSN 500
    FNSRSALPKN NQGGGKALSP IPSPTRNSVR NEDSEGKLTS SSKRNSVPYY 550
    GYAPESSSDR KSSCSSHEEE QFQETMNTFE RPTSSIYADG STIASISNDK 600
    LAHEKEGKKK PTRHSSSLSS KSKSDSRRNS SLKRSSSASR TSSFKKSSFM 650
    LSPFRQQFTD NAARSSSPEE NPITSMPSEK NSSPIVDKKS SKKSRSKRRS 700
    VSAKEAEIFT ETVKDDKNKR SASEAIKGET LKGKSLRQMT ARPVAKKKQT 750
    SAFIEGLRSI SVKEAMKDAD FSGWMSKKGS GAMSTWKTRF FTLHGTRLSY 800
    FSSTTDTRER GLIDITAHRV VPAKEDDKLV SLYAASTGKG RYCFKLLPPQ 850
    PGSKKGLTFT QPRTHYFAVD NKEEMRGWMA ALIKTTIDID TSVPIISSYT 900
    TPTVSLSKAQ EMLAEAREET KLREQQMLEN EEDEDQFLWD QQQLQQQQHD 950
    NNQGQADRTI SASTQRTSDE DNTISTPNLS SANNTTIGSN GFSSPFLLAS 1000
    GLLSPGVARN SSMRGTEKKG KFSTEEDYFG DNSKHKTDKI 1040
    Length:1,040
    Mass (Da):115,688
    Last modified:February 1, 1995 - v1
    Checksum:i2DC635B1A34E7479
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti733 – 7331G → A in BAA07427. (PubMed:8666671)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38310 Genomic DNA. Translation: BAA07427.1.
    U18916 Genomic DNA. Translation: AAC03212.1.
    BK006939 Genomic DNA. Translation: DAA07774.1.
    PIRiS50617.
    RefSeqiNP_011039.1. NM_001179004.1.

    Genome annotation databases

    EnsemblFungiiYER114C; YER114C; YER114C.
    GeneIDi856850.
    KEGGisce:YER114C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38310 Genomic DNA. Translation: BAA07427.1 .
    U18916 Genomic DNA. Translation: AAC03212.1 .
    BK006939 Genomic DNA. Translation: DAA07774.1 .
    PIRi S50617.
    RefSeqi NP_011039.1. NM_001179004.1.

    3D structure databases

    ProteinModelPortali P39969.
    SMRi P39969. Positions 48-105.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36859. 71 interactions.
    DIPi DIP-2227N.
    IntActi P39969. 33 interactions.
    MINTi MINT-537009.
    STRINGi 4932.YER114C.

    Proteomic databases

    MaxQBi P39969.
    PaxDbi P39969.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER114C ; YER114C ; YER114C .
    GeneIDi 856850.
    KEGGi sce:YER114C.

    Organism-specific databases

    CYGDi YER114c.
    SGDi S000000916. BOI2.

    Phylogenomic databases

    eggNOGi NOG73665.
    GeneTreei ENSGT00530000066577.
    HOGENOMi HOG000066063.
    OMAi SITHETI.
    OrthoDBi EOG7N63W3.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30278-MONOMER.

    Miscellaneous databases

    NextBioi 983183.

    Gene expression databases

    Genevestigatori P39969.

    Family and domain databases

    Gene3Di 1.10.150.50. 1 hit.
    2.30.29.30. 1 hit.
    InterProi IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR001660. SAM.
    IPR013761. SAM/pointed.
    IPR011510. SAM_2.
    IPR001452. SH3_domain.
    [Graphical view ]
    Pfami PF00169. PH. 1 hit.
    PF07647. SAM_2. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view ]
    SMARTi SM00233. PH. 1 hit.
    SM00454. SAM. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47769. SSF47769. 1 hit.
    SSF50044. SSF50044. 1 hit.
    PROSITEi PS50003. PH_DOMAIN. 1 hit.
    PS50105. SAM_DOMAIN. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Yeast src homology region 3 domain-binding proteins involved in bud formation."
      Matsui Y., Matsui R., Akada R., Toh-e A.
      J. Cell Biol. 133:865-878(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18; SER-28; SER-450; SER-519; SER-523 AND SER-652, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    6. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-519, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18; SER-519 AND SER-546, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18; SER-24; SER-519; SER-523 AND SER-546, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiBOI2_YEAST
    AccessioniPrimary (citable) accession number: P39969
    Secondary accession number(s): D3DM20
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 134 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 688 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3