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P39968 (VAC8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vacuolar protein 8
Gene names
Name:VAC8
Synonyms:YEB3
Ordered Locus Names:YEL013W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length578 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole. Ref.4

Subcellular location

Vacuole membrane; Lipid-anchor Ref.4.

Post-translational modification

Palmitoylated on one or more of its N-terminal cysteine residues by PFA3, which is required for vacuole fusion. Ref.4 Ref.6 Ref.8

Miscellaneous

Present with 5680 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the beta-catenin family.

Contains 9 ARM repeats.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 578577Vacuolar protein 8
PRO_0000064302

Regions

Repeat37 – 7539ARM 1
Repeat76 – 11439ARM 2
Repeat116 – 15540ARM 3
Repeat157 – 19640ARM 4
Repeat198 – 23740ARM 5
Repeat239 – 28042ARM 6
Repeat282 – 32140ARM 7
Repeat323 – 36341ARM 8
Repeat407 – 44640ARM 9

Amino acid modifications

Modified residue111Phosphoserine Ref.11
Modified residue161Phosphoserine Ref.9 Ref.10 Ref.11
Lipidation21N-myristoyl glycine Ref.4
Lipidation41S-palmitoyl cysteine Probable
Lipidation51S-palmitoyl cysteine Probable
Lipidation71S-palmitoyl cysteine Probable

Experimental info

Mutagenesis4 – 74CCSC → GTSS in VAC8-3; not palmitoylated. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P39968 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: B2E75774B47E5933

FASTA57863,208
        10         20         30         40         50         60 
MGSCCSCLKD SSDEASVSPI ADNEREAVTL LLGYLEDKDQ LDFYSGGPLK ALTTLVYSDN 

        70         80         90        100        110        120 
LNLQRSAALA FAEITEKYVR QVSREVLEPI LILLQSQDPQ IQVAACAALG NLAVNNENKL 

       130        140        150        160        170        180 
LIVEMGGLEP LINQMMGDNV EVQCNAVGCI TNLATRDDNK HKIATSGALI PLTKLAKSKH 

       190        200        210        220        230        240 
IRVQRNATGA LLNMTHSEEN RKELVNAGAV PVLVSLLSST DPDVQYYCTT ALSNIAVDEA 

       250        260        270        280        290        300 
NRKKLAQTEP RLVSKLVSLM DSPSSRVKCQ ATLALRNLAS DTSYQLEIVR AGGLPHLVKL 

       310        320        330        340        350        360 
IQSDSIPLVL ASVACIRNIS IHPLNEGLIV DAGFLKPLVR LLDYKDSEEI QCHAVSTLRN 

       370        380        390        400        410        420 
LAASSEKNRK EFFESGAVEK CKELALDSPV SVQSEISACF AILALADVSK LDLLEANILD 

       430        440        450        460        470        480 
ALIPMTFSQN QEVSGNAAAA LANLCSRVNN YTKIIEAWDR PNEGIRGFLI RFLKSDYATF 

       490        500        510        520        530        540 
EHIALWTILQ LLESHNDKVE DLVKNDDDII NGVRKMADVT FERLQRSGID VKNPGSNNNP 

       550        560        570 
SSNDNNSNNN DTGSEHQPVE DASLELYNIT QQILQFLH 

« Hide

References

« Hide 'large scale' references
[1]"New members of the plakoglobin/armadillo/beta-catenin family characterized by armadillo repeats."
Fischer R.
Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Vac8p, a vacuolar protein with armadillo repeats, functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole."
Wang Y.-X., Catlett N.L., Weisman L.S.
J. Cell Biol. 140:1063-1074(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MYRISTOYLATION AT GLY-2, PALMITOYLATION AT CYS-4; CYS-5 AND CYS-7, MUTAGENESIS OF 4-CYS--CYS-7, SUBCELLULAR LOCATION.
[5]"Apg13p and Vac8p are part of a complex of phosphoproteins that are required for cytoplasm to vacuole targeting."
Scott S.V., Nice D.C. III, Nau J.J., Weisman L.S., Kamada Y., Keizer-Gunnink I., Funakoshi T., Veenhuis M., Ohsumi Y., Klionsky D.J.
J. Biol. Chem. 275:25840-25849(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION, INTERACTION WITH ATG13.
[6]"Vac8p release from the SNARE complex and its palmitoylation are coupled and essential for vacuole fusion."
Veit M., Laage R., Dietrich L., Wang L., Ungermann C.
EMBO J. 20:3145-3155(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: PALMITOYLATION.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p."
Smotrys J.E., Schoenfish M.J., Stutz M.A., Linder M.E.
J. Cell Biol. 170:1091-1099(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PALMITOYLATION.
[9]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11 AND SER-16, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF005267 mRNA. Translation: AAQ13402.1.
U18530 Genomic DNA. Translation: AAB64490.1.
BK006939 Genomic DNA. Translation: DAA07640.1.
PIRS50446.
RefSeqNP_010903.3. NM_001178828.3.

3D structure databases

ProteinModelPortalP39968.
SMRP39968. Positions 46-446.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36717. 105 interactions.
DIPDIP-5121N.
IntActP39968. 20 interactions.
MINTMINT-517641.
STRING4932.YEL013W.

Proteomic databases

MaxQBP39968.
PaxDbP39968.
PeptideAtlasP39968.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYEL013W; YEL013W; YEL013W.
GeneID856702.
KEGGsce:YEL013W.

Organism-specific databases

SGDS000000739. VAC8.

Phylogenomic databases

eggNOGCOG5064.
GeneTreeENSGT00740000116497.
HOGENOMHOG000177331.
KOK08332.
OMAVQCHAIS.
OrthoDBEOG7Q8CX4.

Enzyme and pathway databases

BioCycYEAST:G3O-30139-MONOMER.

Gene expression databases

GenevestigatorP39968.

Family and domain databases

Gene3D1.25.10.10. 1 hit.
InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
[Graphical view]
PfamPF00514. Arm. 8 hits.
[Graphical view]
SMARTSM00185. ARM. 8 hits.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
PROSITEPS50176. ARM_REPEAT. 7 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio982766.

Entry information

Entry nameVAC8_YEAST
AccessionPrimary (citable) accession number: P39968
Secondary accession number(s): D3DLN6, Q549T0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families