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P39968

- VAC8_YEAST

UniProt

P39968 - VAC8_YEAST

Protein

Vacuolar protein 8

Gene

VAC8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole.1 Publication

    GO - Molecular functioni

    1. protein anchor Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. CVT vesicle assembly Source: SGD
    2. macroautophagy Source: SGD
    3. nucleus-vacuole junction assembly Source: SGD
    4. piecemeal microautophagy of nucleus Source: SGD
    5. vacuole fusion, non-autophagic Source: SGD
    6. vacuole inheritance Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30139-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vacuolar protein 8
    Gene namesi
    Name:VAC8
    Synonyms:YEB3
    Ordered Locus Names:YEL013W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    SGDiS000000739. VAC8.

    Subcellular locationi

    Vacuole membrane 1 Publication; Lipid-anchor 1 Publication

    GO - Cellular componenti

    1. fungal-type vacuole membrane Source: SGD
    2. Myo2p-Vac17p-Vac8p transport complex Source: SGD
    3. nucleus-vacuole junction Source: SGD

    Keywords - Cellular componenti

    Membrane, Vacuole

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi4 – 74CCSC → GTSS in VAC8-3; not palmitoylated. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 578577Vacuolar protein 8PRO_0000064302Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycine1 Publication
    Lipidationi4 – 41S-palmitoyl cysteine1 Publication
    Lipidationi5 – 51S-palmitoyl cysteine1 Publication
    Lipidationi7 – 71S-palmitoyl cysteine1 Publication
    Modified residuei11 – 111Phosphoserine2 Publications
    Modified residuei16 – 161Phosphoserine4 Publications

    Post-translational modificationi

    Palmitoylated on one or more of its N-terminal cysteine residues by PFA3, which is required for vacuole fusion.3 Publications

    Keywords - PTMi

    Lipoprotein, Myristate, Palmitate, Phosphoprotein

    Proteomic databases

    MaxQBiP39968.
    PaxDbiP39968.
    PeptideAtlasiP39968.

    Expressioni

    Gene expression databases

    GenevestigatoriP39968.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ATG13Q066285EBI-20212,EBI-36188
    NVJ1P388814EBI-20212,EBI-24885
    VAB2P400032EBI-20212,EBI-22275

    Protein-protein interaction databases

    BioGridi36717. 105 interactions.
    DIPiDIP-5121N.
    IntActiP39968. 20 interactions.
    MINTiMINT-517641.
    STRINGi4932.YEL013W.

    Structurei

    3D structure databases

    ProteinModelPortaliP39968.
    SMRiP39968. Positions 46-446.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati37 – 7539ARM 1Add
    BLAST
    Repeati76 – 11439ARM 2Add
    BLAST
    Repeati116 – 15540ARM 3Add
    BLAST
    Repeati157 – 19640ARM 4Add
    BLAST
    Repeati198 – 23740ARM 5Add
    BLAST
    Repeati239 – 28042ARM 6Add
    BLAST
    Repeati282 – 32140ARM 7Add
    BLAST
    Repeati323 – 36341ARM 8Add
    BLAST
    Repeati407 – 44640ARM 9Add
    BLAST

    Sequence similaritiesi

    Belongs to the beta-catenin family.Curated
    Contains 9 ARM repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5064.
    GeneTreeiENSGT00740000116497.
    HOGENOMiHOG000177331.
    KOiK08332.
    OMAiVQCHAIS.
    OrthoDBiEOG7Q8CX4.

    Family and domain databases

    Gene3Di1.25.10.10. 1 hit.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR000225. Armadillo.
    [Graphical view]
    PfamiPF00514. Arm. 8 hits.
    [Graphical view]
    SMARTiSM00185. ARM. 8 hits.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.
    PROSITEiPS50176. ARM_REPEAT. 7 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P39968-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSCCSCLKD SSDEASVSPI ADNEREAVTL LLGYLEDKDQ LDFYSGGPLK    50
    ALTTLVYSDN LNLQRSAALA FAEITEKYVR QVSREVLEPI LILLQSQDPQ 100
    IQVAACAALG NLAVNNENKL LIVEMGGLEP LINQMMGDNV EVQCNAVGCI 150
    TNLATRDDNK HKIATSGALI PLTKLAKSKH IRVQRNATGA LLNMTHSEEN 200
    RKELVNAGAV PVLVSLLSST DPDVQYYCTT ALSNIAVDEA NRKKLAQTEP 250
    RLVSKLVSLM DSPSSRVKCQ ATLALRNLAS DTSYQLEIVR AGGLPHLVKL 300
    IQSDSIPLVL ASVACIRNIS IHPLNEGLIV DAGFLKPLVR LLDYKDSEEI 350
    QCHAVSTLRN LAASSEKNRK EFFESGAVEK CKELALDSPV SVQSEISACF 400
    AILALADVSK LDLLEANILD ALIPMTFSQN QEVSGNAAAA LANLCSRVNN 450
    YTKIIEAWDR PNEGIRGFLI RFLKSDYATF EHIALWTILQ LLESHNDKVE 500
    DLVKNDDDII NGVRKMADVT FERLQRSGID VKNPGSNNNP SSNDNNSNNN 550
    DTGSEHQPVE DASLELYNIT QQILQFLH 578
    Length:578
    Mass (Da):63,208
    Last modified:January 23, 2007 - v3
    Checksum:iB2E75774B47E5933
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF005267 mRNA. Translation: AAQ13402.1.
    U18530 Genomic DNA. Translation: AAB64490.1.
    BK006939 Genomic DNA. Translation: DAA07640.1.
    PIRiS50446.
    RefSeqiNP_010903.3. NM_001178828.3.

    Genome annotation databases

    EnsemblFungiiYEL013W; YEL013W; YEL013W.
    GeneIDi856702.
    KEGGisce:YEL013W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF005267 mRNA. Translation: AAQ13402.1 .
    U18530 Genomic DNA. Translation: AAB64490.1 .
    BK006939 Genomic DNA. Translation: DAA07640.1 .
    PIRi S50446.
    RefSeqi NP_010903.3. NM_001178828.3.

    3D structure databases

    ProteinModelPortali P39968.
    SMRi P39968. Positions 46-446.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36717. 105 interactions.
    DIPi DIP-5121N.
    IntActi P39968. 20 interactions.
    MINTi MINT-517641.
    STRINGi 4932.YEL013W.

    Proteomic databases

    MaxQBi P39968.
    PaxDbi P39968.
    PeptideAtlasi P39968.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YEL013W ; YEL013W ; YEL013W .
    GeneIDi 856702.
    KEGGi sce:YEL013W.

    Organism-specific databases

    SGDi S000000739. VAC8.

    Phylogenomic databases

    eggNOGi COG5064.
    GeneTreei ENSGT00740000116497.
    HOGENOMi HOG000177331.
    KOi K08332.
    OMAi VQCHAIS.
    OrthoDBi EOG7Q8CX4.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30139-MONOMER.

    Miscellaneous databases

    NextBioi 982766.

    Gene expression databases

    Genevestigatori P39968.

    Family and domain databases

    Gene3Di 1.25.10.10. 1 hit.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR000225. Armadillo.
    [Graphical view ]
    Pfami PF00514. Arm. 8 hits.
    [Graphical view ]
    SMARTi SM00185. ARM. 8 hits.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    PROSITEi PS50176. ARM_REPEAT. 7 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "New members of the plakoglobin/armadillo/beta-catenin family characterized by armadillo repeats."
      Fischer R.
      Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Vac8p, a vacuolar protein with armadillo repeats, functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole."
      Wang Y.-X., Catlett N.L., Weisman L.S.
      J. Cell Biol. 140:1063-1074(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MYRISTOYLATION AT GLY-2, PALMITOYLATION AT CYS-4; CYS-5 AND CYS-7, MUTAGENESIS OF 4-CYS--CYS-7, SUBCELLULAR LOCATION.
    5. "Apg13p and Vac8p are part of a complex of phosphoproteins that are required for cytoplasm to vacuole targeting."
      Scott S.V., Nice D.C. III, Nau J.J., Weisman L.S., Kamada Y., Keizer-Gunnink I., Funakoshi T., Veenhuis M., Ohsumi Y., Klionsky D.J.
      J. Biol. Chem. 275:25840-25849(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION, INTERACTION WITH ATG13.
    6. "Vac8p release from the SNARE complex and its palmitoylation are coupled and essential for vacuole fusion."
      Veit M., Laage R., Dietrich L., Wang L., Ungermann C.
      EMBO J. 20:3145-3155(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PALMITOYLATION.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p."
      Smotrys J.E., Schoenfish M.J., Stutz M.A., Linder M.E.
      J. Cell Biol. 170:1091-1099(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PALMITOYLATION.
    9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11 AND SER-16, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiVAC8_YEAST
    AccessioniPrimary (citable) accession number: P39968
    Secondary accession number(s): D3DLN6, Q549T0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 128 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 5680 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3