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Protein

Vacuolar protein 8

Gene

VAC8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole.1 Publication

Miscellaneous

Present with 5680 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • protein membrane anchor Source: SGD

GO - Biological processi

  • Cvt vesicle assembly Source: SGD
  • macroautophagy Source: SGD
  • nucleus-vacuole junction assembly Source: SGD
  • piecemeal microautophagy of nucleus Source: SGD
  • vacuole fusion, non-autophagic Source: SGD
  • vacuole inheritance Source: SGD

Enzyme and pathway databases

BioCyciYEAST:G3O-30139-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein 8
Gene namesi
Name:VAC8
Synonyms:YEB3
Ordered Locus Names:YEL013W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL013W.
SGDiS000000739. VAC8.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Vacuole

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi4 – 7CCSC → GTSS in VAC8-3; not palmitoylated. 1 Publication4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000643022 – 578Vacuolar protein 8Add BLAST577

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1
Lipidationi4S-palmitoyl cysteine1 Publication1
Lipidationi5S-palmitoyl cysteine1 Publication1
Lipidationi7S-palmitoyl cysteine1 Publication1
Modified residuei11PhosphoserineCombined sources1
Modified residuei16PhosphoserineCombined sources1
Cross-linki77Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki515Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Post-translational modificationi

Palmitoylated on one or more of its N-terminal cysteine residues by PFA3, which is required for vacuole fusion.3 Publications

Keywords - PTMi

Isopeptide bond, Lipoprotein, Myristate, Palmitate, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP39968.
PRIDEiP39968.

PTM databases

iPTMnetiP39968.
SwissPalmiP39968.

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein membrane anchor Source: SGD

Protein-protein interaction databases

BioGridi36717. 328 interactors.
DIPiDIP-5121N.
IntActiP39968. 24 interactors.
MINTiMINT-517641.
STRINGi4932.YEL013W.

Structurei

Secondary structure

1578
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi21 – 33Combined sources13
Beta strandi43 – 45Combined sources3
Helixi46 – 56Combined sources11
Helixi61 – 77Combined sources17
Helixi84 – 93Combined sources10
Helixi99 – 112Combined sources14
Helixi116 – 124Combined sources9
Turni125 – 127Combined sources3
Helixi128 – 135Combined sources8
Helixi140 – 153Combined sources14
Helixi157 – 164Combined sources8
Turni165 – 168Combined sources4
Helixi169 – 175Combined sources7
Helixi181 – 193Combined sources13
Helixi198 – 207Combined sources10
Helixi209 – 216Combined sources8
Helixi222 – 235Combined sources14
Helixi239 – 248Combined sources10
Helixi252 – 259Combined sources8
Helixi265 – 278Combined sources14
Helixi282 – 290Combined sources9
Helixi293 – 301Combined sources9
Helixi306 – 319Combined sources14
Helixi323 – 325Combined sources3
Helixi326 – 331Combined sources6
Helixi335 – 340Combined sources6
Helixi341 – 343Combined sources3
Helixi348 – 360Combined sources13
Helixi366 – 373Combined sources8
Turni374 – 376Combined sources3
Helixi377 – 384Combined sources8
Helixi390 – 403Combined sources14
Helixi407 – 409Combined sources3
Helixi410 – 415Combined sources6
Turni416 – 418Combined sources3
Helixi419 – 425Combined sources7
Helixi431 – 444Combined sources14
Helixi452 – 456Combined sources5
Helixi464 – 474Combined sources11
Helixi478 – 493Combined sources16
Helixi497 – 503Combined sources7
Helixi506 – 511Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5XJGX-ray2.40A/C10-515[»]
ProteinModelPortaliP39968.
SMRiP39968.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati37 – 75ARM 1Add BLAST39
Repeati76 – 114ARM 2Add BLAST39
Repeati116 – 155ARM 3Add BLAST40
Repeati157 – 196ARM 4Add BLAST40
Repeati198 – 237ARM 5Add BLAST40
Repeati239 – 280ARM 6Add BLAST42
Repeati282 – 321ARM 7Add BLAST40
Repeati323 – 363ARM 8Add BLAST41
Repeati407 – 446ARM 9Add BLAST40

Sequence similaritiesi

Belongs to the beta-catenin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00770000120927.
HOGENOMiHOG000177331.
InParanoidiP39968.
KOiK08332.
OMAiATFQHIA.
OrthoDBiEOG092C1KQT.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
InterProiView protein in InterPro
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
PfamiView protein in Pfam
PF00514. Arm. 8 hits.
SMARTiView protein in SMART
SM00185. ARM. 9 hits.
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiView protein in PROSITE
PS50176. ARM_REPEAT. 7 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39968-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSCCSCLKD SSDEASVSPI ADNEREAVTL LLGYLEDKDQ LDFYSGGPLK
60 70 80 90 100
ALTTLVYSDN LNLQRSAALA FAEITEKYVR QVSREVLEPI LILLQSQDPQ
110 120 130 140 150
IQVAACAALG NLAVNNENKL LIVEMGGLEP LINQMMGDNV EVQCNAVGCI
160 170 180 190 200
TNLATRDDNK HKIATSGALI PLTKLAKSKH IRVQRNATGA LLNMTHSEEN
210 220 230 240 250
RKELVNAGAV PVLVSLLSST DPDVQYYCTT ALSNIAVDEA NRKKLAQTEP
260 270 280 290 300
RLVSKLVSLM DSPSSRVKCQ ATLALRNLAS DTSYQLEIVR AGGLPHLVKL
310 320 330 340 350
IQSDSIPLVL ASVACIRNIS IHPLNEGLIV DAGFLKPLVR LLDYKDSEEI
360 370 380 390 400
QCHAVSTLRN LAASSEKNRK EFFESGAVEK CKELALDSPV SVQSEISACF
410 420 430 440 450
AILALADVSK LDLLEANILD ALIPMTFSQN QEVSGNAAAA LANLCSRVNN
460 470 480 490 500
YTKIIEAWDR PNEGIRGFLI RFLKSDYATF EHIALWTILQ LLESHNDKVE
510 520 530 540 550
DLVKNDDDII NGVRKMADVT FERLQRSGID VKNPGSNNNP SSNDNNSNNN
560 570
DTGSEHQPVE DASLELYNIT QQILQFLH
Length:578
Mass (Da):63,208
Last modified:January 23, 2007 - v3
Checksum:iB2E75774B47E5933
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF005267 mRNA. Translation: AAQ13402.1.
U18530 Genomic DNA. Translation: AAB64490.1.
BK006939 Genomic DNA. Translation: DAA07640.1.
PIRiS50446.
RefSeqiNP_010903.3. NM_001178828.3.

Genome annotation databases

EnsemblFungiiYEL013W; YEL013W; YEL013W.
GeneIDi856702.
KEGGisce:YEL013W.

Similar proteinsi

Entry informationi

Entry nameiVAC8_YEAST
AccessioniPrimary (citable) accession number: P39968
Secondary accession number(s): D3DLN6, Q549T0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: November 22, 2017
This is version 158 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names