Vacuolar protein 8 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P39968 (VAC8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 116. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Vacuolar protein 8

Gene names

Name:	VAC8
Synonyms:	YEB3
Ordered Locus Names:	YEL013W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	578 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole. Ref.4
Subcellular location	Vacuole membrane; Lipid-anchor Ref.4.
Post-translational modification	Palmitoylated on one or more of its N-terminal cysteine residues by PFA3, which is required for vacuole fusion. Ref.4 Ref.6 Ref.8
Miscellaneous	Present with 5680 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the beta-catenin family. Contains 9 ARM repeats.

Ontologies

Keywords
Cellular component	Membrane Vacuole
Domain	Repeat
PTM	Lipoprotein Myristate Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	CVT vesicle assembly Inferred from mutant phenotype Ref.5. Source: SGD macroautophagy Inferred from mutant phenotype PubMed 15901835. Source: SGD nucleus-vacuole junction assembly Inferred from mutant phenotype PubMed 10888680. Source: SGD vacuole fusion, non-autophagic Inferred from mutant phenotype PubMed 11441010 PubMed 16301533. Source: SGD vacuole inheritance Inferred from mutant phenotype PubMed 16301533 PubMed 8885233. Source: SGD
Cellular_component	Myo2p-Vac17p-Vac8p transport complex Inferred from direct assay PubMed 12529432. Source: SGD fungal-type vacuole membrane Inferred from direct assay PubMed 16301533 Ref.4 PubMed 9664035 PubMed 9739084. Source: SGD nucleus-vacuole junction Inferred from direct assay PubMed 10888680. Source: SGD
Molecular_function	protein anchor Inferred from mutant phenotype PubMed 10888680 PubMed 12594460. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
ATG13	Q06628	5	EBI-20212,EBI-36188
NVJ1	P38881	4	EBI-20212,EBI-24885
VAB2	P40003	2	EBI-20212,EBI-22275

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed

Chain

2 – 578

577

Vacuolar protein 8

PRO_0000064302

Regions

Repeat

37 – 75

ARM 1

Repeat

76 – 114

ARM 2

Repeat

116 – 155

ARM 3

Repeat

157 – 196

ARM 4

Repeat

198 – 237

ARM 5

Repeat

239 – 280

ARM 6

Repeat

282 – 321

ARM 7

Repeat

323 – 363

ARM 8

Repeat

407 – 446

ARM 9

Amino acid modifications

Modified residue

Phosphoserine Ref.9 Ref.10

Modified residue

Phosphoserine Ref.10

Lipidation

N-myristoyl glycine Ref.4

Lipidation

S-palmitoyl cysteine Probable

Lipidation

S-palmitoyl cysteine Probable

Lipidation

S-palmitoyl cysteine Probable

Experimental info

Mutagenesis

4 – 7

CCSC → GTSS in VAC8-3; not palmitoylated. Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

P39968 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: B2E75774B47E5933
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FASTA

578

63,208

        10         20         30         40         50         60 
MGSCCSCLKD SSDEASVSPI ADNEREAVTL LLGYLEDKDQ LDFYSGGPLK ALTTLVYSDN 

        70         80         90        100        110        120 
LNLQRSAALA FAEITEKYVR QVSREVLEPI LILLQSQDPQ IQVAACAALG NLAVNNENKL 

       130        140        150        160        170        180 
LIVEMGGLEP LINQMMGDNV EVQCNAVGCI TNLATRDDNK HKIATSGALI PLTKLAKSKH 

       190        200        210        220        230        240 
IRVQRNATGA LLNMTHSEEN RKELVNAGAV PVLVSLLSST DPDVQYYCTT ALSNIAVDEA 

       250        260        270        280        290        300 
NRKKLAQTEP RLVSKLVSLM DSPSSRVKCQ ATLALRNLAS DTSYQLEIVR AGGLPHLVKL 

       310        320        330        340        350        360 
IQSDSIPLVL ASVACIRNIS IHPLNEGLIV DAGFLKPLVR LLDYKDSEEI QCHAVSTLRN 

       370        380        390        400        410        420 
LAASSEKNRK EFFESGAVEK CKELALDSPV SVQSEISACF AILALADVSK LDLLEANILD 

       430        440        450        460        470        480 
ALIPMTFSQN QEVSGNAAAA LANLCSRVNN YTKIIEAWDR PNEGIRGFLI RFLKSDYATF 

       490        500        510        520        530        540 
EHIALWTILQ LLESHNDKVE DLVKNDDDII NGVRKMADVT FERLQRSGID VKNPGSNNNP 

       550        560        570 
SSNDNNSNNN DTGSEHQPVE DASLELYNIT QQILQFLH

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"New members of the plakoglobin/armadillo/beta-catenin family characterized by armadillo repeats." Fischer R. Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Vac8p, a vacuolar protein with armadillo repeats, functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole." Wang Y.-X., Catlett N.L., Weisman L.S. J. Cell Biol. 140:1063-1074(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MYRISTOYLATION AT GLY-2, PALMITOYLATION AT CYS-4; CYS-5 AND CYS-7, MUTAGENESIS OF 4-CYS--CYS-7, SUBCELLULAR LOCATION.
[5]	"Apg13p and Vac8p are part of a complex of phosphoproteins that are required for cytoplasm to vacuole targeting." Scott S.V., Nice D.C. III, Nau J.J., Weisman L.S., Kamada Y., Keizer-Gunnink I., Funakoshi T., Veenhuis M., Ohsumi Y., Klionsky D.J. J. Biol. Chem. 275:25840-25849(2000) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION, INTERACTION WITH ATG13.
[6]	"Vac8p release from the SNARE complex and its palmitoylation are coupled and essential for vacuole fusion." Veit M., Laage R., Dietrich L., Wang L., Ungermann C. EMBO J. 20:3145-3155(2001) [PubMed] [Europe PMC] [Abstract] Cited for: PALMITOYLATION.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p." Smotrys J.E., Schoenfish M.J., Stutz M.A., Linder M.E. J. Cell Biol. 170:1091-1099(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PALMITOYLATION.
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16, MASS SPECTROMETRY. Strain: ADR376.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16 AND SER-18, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF005267 mRNA. Translation: AAQ13402.1. U18530 Genomic DNA. Translation: AAB64490.1. BK006939 Genomic DNA. Translation: DAA07640.1.
PIR	S50446.
RefSeq	NP_010903.3. NM_001178828.3.
3D structure databases
ProteinModelPortal	P39968.
SMR	P39968. Positions 48-495.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5121N.
IntAct	P39968. 21 interactions.
MINT	MINT-517641.
STRING	4932.YEL013W.
Proteomic databases
PaxDb	P39968.
PeptideAtlas	P39968.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YEL013W; YEL013W; YEL013W.
GeneID	856702.
KEGG	sce:YEL009C. sce:YEL013W.
Organism-specific databases
SGD	S000000739. VAC8.
Phylogenomic databases
eggNOG	COG5064.
GeneTree	ENSGT00680000099702.
HOGENOM	HOG000177331.
KO	K08332. K09464.
OMA	EEIQCHA.
OrthoDB	EOG4QZBV8.
Gene expression databases
Genevestigator	P39968.
GermOnline	YEL013W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.25.10.10. 1 hit.
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR000225. Armadillo. IPR026958. Vac8. [Graphical view]
PANTHER	PTHR23315:SF8. PTHR23315:SF8. 1 hit.
Pfam	PF00514. Arm. 8 hits. [Graphical view]
SMART	SM00185. ARM. 8 hits. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS50176. ARM_REPEAT. 7 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	982766.

Entry information

Entry name

VAC8_YEAST

Accession

Primary (citable) accession number: P39968
Secondary accession number(s): D3DLN6, Q549T0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 116 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents