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Protein

Vacuolar protein 8

Gene

VAC8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole.1 Publication

GO - Molecular functioni

  • protein anchor Source: SGD

GO - Biological processi

  • CVT vesicle assembly Source: SGD
  • macroautophagy Source: SGD
  • nucleus-vacuole junction assembly Source: SGD
  • piecemeal microautophagy of nucleus Source: SGD
  • vacuole fusion, non-autophagic Source: SGD
  • vacuole inheritance Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-30139-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein 8
Gene namesi
Name:VAC8
Synonyms:YEB3
Ordered Locus Names:YEL013W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL013W.
SGDiS000000739. VAC8.

Subcellular locationi

  • Vacuole membrane 1 Publication; Lipid-anchor 1 Publication

GO - Cellular componenti

  • fungal-type vacuole membrane Source: SGD
  • Myo2p-Vac17p-Vac8p transport complex Source: SGD
  • nucleus Source: GO_Central
  • nucleus-vacuole junction Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi4 – 7CCSC → GTSS in VAC8-3; not palmitoylated. 1 Publication4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000643022 – 578Vacuolar protein 8Add BLAST577

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1
Lipidationi4S-palmitoyl cysteine1 Publication1
Lipidationi5S-palmitoyl cysteine1 Publication1
Lipidationi7S-palmitoyl cysteine1 Publication1
Modified residuei11PhosphoserineCombined sources1
Modified residuei16PhosphoserineCombined sources1
Cross-linki77Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki515Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Post-translational modificationi

Palmitoylated on one or more of its N-terminal cysteine residues by PFA3, which is required for vacuole fusion.3 Publications

Keywords - PTMi

Isopeptide bond, Lipoprotein, Myristate, Palmitate, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP39968.
PRIDEiP39968.

PTM databases

iPTMnetiP39968.
SwissPalmiP39968.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ATG13Q066285EBI-20212,EBI-36188
NVJ1P388814EBI-20212,EBI-24885
VAB2P400032EBI-20212,EBI-22275

GO - Molecular functioni

  • protein anchor Source: SGD

Protein-protein interaction databases

BioGridi36717. 108 interactors.
DIPiDIP-5121N.
IntActiP39968. 20 interactors.
MINTiMINT-517641.

Structurei

3D structure databases

ProteinModelPortaliP39968.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati37 – 75ARM 1Add BLAST39
Repeati76 – 114ARM 2Add BLAST39
Repeati116 – 155ARM 3Add BLAST40
Repeati157 – 196ARM 4Add BLAST40
Repeati198 – 237ARM 5Add BLAST40
Repeati239 – 280ARM 6Add BLAST42
Repeati282 – 321ARM 7Add BLAST40
Repeati323 – 363ARM 8Add BLAST41
Repeati407 – 446ARM 9Add BLAST40

Sequence similaritiesi

Belongs to the beta-catenin family.Curated
Contains 9 ARM repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00770000120927.
HOGENOMiHOG000177331.
InParanoidiP39968.
KOiK08332.
OMAiHIAVWTI.
OrthoDBiEOG092C1KQT.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
[Graphical view]
PfamiPF00514. Arm. 8 hits.
[Graphical view]
SMARTiSM00185. ARM. 9 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 7 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39968-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSCCSCLKD SSDEASVSPI ADNEREAVTL LLGYLEDKDQ LDFYSGGPLK
60 70 80 90 100
ALTTLVYSDN LNLQRSAALA FAEITEKYVR QVSREVLEPI LILLQSQDPQ
110 120 130 140 150
IQVAACAALG NLAVNNENKL LIVEMGGLEP LINQMMGDNV EVQCNAVGCI
160 170 180 190 200
TNLATRDDNK HKIATSGALI PLTKLAKSKH IRVQRNATGA LLNMTHSEEN
210 220 230 240 250
RKELVNAGAV PVLVSLLSST DPDVQYYCTT ALSNIAVDEA NRKKLAQTEP
260 270 280 290 300
RLVSKLVSLM DSPSSRVKCQ ATLALRNLAS DTSYQLEIVR AGGLPHLVKL
310 320 330 340 350
IQSDSIPLVL ASVACIRNIS IHPLNEGLIV DAGFLKPLVR LLDYKDSEEI
360 370 380 390 400
QCHAVSTLRN LAASSEKNRK EFFESGAVEK CKELALDSPV SVQSEISACF
410 420 430 440 450
AILALADVSK LDLLEANILD ALIPMTFSQN QEVSGNAAAA LANLCSRVNN
460 470 480 490 500
YTKIIEAWDR PNEGIRGFLI RFLKSDYATF EHIALWTILQ LLESHNDKVE
510 520 530 540 550
DLVKNDDDII NGVRKMADVT FERLQRSGID VKNPGSNNNP SSNDNNSNNN
560 570
DTGSEHQPVE DASLELYNIT QQILQFLH
Length:578
Mass (Da):63,208
Last modified:January 23, 2007 - v3
Checksum:iB2E75774B47E5933
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF005267 mRNA. Translation: AAQ13402.1.
U18530 Genomic DNA. Translation: AAB64490.1.
BK006939 Genomic DNA. Translation: DAA07640.1.
PIRiS50446.
RefSeqiNP_010903.3. NM_001178828.3.

Genome annotation databases

EnsemblFungiiYEL013W; YEL013W; YEL013W.
GeneIDi856702.
KEGGisce:YEL013W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF005267 mRNA. Translation: AAQ13402.1.
U18530 Genomic DNA. Translation: AAB64490.1.
BK006939 Genomic DNA. Translation: DAA07640.1.
PIRiS50446.
RefSeqiNP_010903.3. NM_001178828.3.

3D structure databases

ProteinModelPortaliP39968.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36717. 108 interactors.
DIPiDIP-5121N.
IntActiP39968. 20 interactors.
MINTiMINT-517641.

PTM databases

iPTMnetiP39968.
SwissPalmiP39968.

Proteomic databases

MaxQBiP39968.
PRIDEiP39968.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYEL013W; YEL013W; YEL013W.
GeneIDi856702.
KEGGisce:YEL013W.

Organism-specific databases

EuPathDBiFungiDB:YEL013W.
SGDiS000000739. VAC8.

Phylogenomic databases

GeneTreeiENSGT00770000120927.
HOGENOMiHOG000177331.
InParanoidiP39968.
KOiK08332.
OMAiHIAVWTI.
OrthoDBiEOG092C1KQT.

Enzyme and pathway databases

BioCyciYEAST:G3O-30139-MONOMER.

Miscellaneous databases

PROiP39968.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
[Graphical view]
PfamiPF00514. Arm. 8 hits.
[Graphical view]
SMARTiSM00185. ARM. 9 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 7 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVAC8_YEAST
AccessioniPrimary (citable) accession number: P39968
Secondary accession number(s): D3DLN6, Q549T0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 148 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5680 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.