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Reviewed, UniProtKB/Swiss-Prot P39968 (VAC8_YEAST)

Last modified November 24, 2009. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Vacuolar protein 8
Gene names
Name: VAC8
Synonyms: YEB3
Ordered Locus Names: YEL013W
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length578 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole. Ref.3

Subcellular location

Vacuole membrane; Lipid-anchor.

Post-translational modification

Palmitoylated on one or more of its N-terminal cysteine residues by PFA3, which is required for vacuole fusion. Ref.3 Ref.5 Ref.7

Phosphorylated. Ref.4 Ref.8 Ref.9

Miscellaneous

Present with 5680 molecules/cell in log phase SD medium. Ref.6

Sequence similarities

Belongs to the beta-catenin family.

Contains 9 ARM repeats.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 578577Vacuolar protein 8
PRO_0000064302

Regions

Repeat37 – 7539ARM 1
Repeat76 – 11439ARM 2
Repeat116 – 15540ARM 3
Repeat157 – 19640ARM 4
Repeat198 – 23740ARM 5
Repeat239 – 28042ARM 6
Repeat282 – 32140ARM 7
Repeat323 – 36341ARM 8
Repeat407 – 44640ARM 9

Amino acid modifications

Modified residue161Phosphoserine Ref.8 Ref.9
Modified residue181Phosphoserine Ref.9
Lipidation21N-myristoyl glycine
Lipidation41S-palmitoyl cysteine Probable
Lipidation51S-palmitoyl cysteine Probable
Lipidation71S-palmitoyl cysteine Probable

Experimental info

Mutagenesis4 – 74CCSC → GTSS in VAC8-3; not palmitoylated. Ref.3

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Sequences

Sequence LengthMass (Da)Tools
P39968-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: B2E75774B47E5933

FASTA57863,208
        10         20         30         40         50         60 
MGSCCSCLKD SSDEASVSPI ADNEREAVTL LLGYLEDKDQ LDFYSGGPLK ALTTLVYSDN 

        70         80         90        100        110        120 
LNLQRSAALA FAEITEKYVR QVSREVLEPI LILLQSQDPQ IQVAACAALG NLAVNNENKL 

       130        140        150        160        170        180 
LIVEMGGLEP LINQMMGDNV EVQCNAVGCI TNLATRDDNK HKIATSGALI PLTKLAKSKH 

       190        200        210        220        230        240 
IRVQRNATGA LLNMTHSEEN RKELVNAGAV PVLVSLLSST DPDVQYYCTT ALSNIAVDEA 

       250        260        270        280        290        300 
NRKKLAQTEP RLVSKLVSLM DSPSSRVKCQ ATLALRNLAS DTSYQLEIVR AGGLPHLVKL 

       310        320        330        340        350        360 
IQSDSIPLVL ASVACIRNIS IHPLNEGLIV DAGFLKPLVR LLDYKDSEEI QCHAVSTLRN 

       370        380        390        400        410        420 
LAASSEKNRK EFFESGAVEK CKELALDSPV SVQSEISACF AILALADVSK LDLLEANILD 

       430        440        450        460        470        480 
ALIPMTFSQN QEVSGNAAAA LANLCSRVNN YTKIIEAWDR PNEGIRGFLI RFLKSDYATF 

       490        500        510        520        530        540 
EHIALWTILQ LLESHNDKVE DLVKNDDDII NGVRKMADVT FERLQRSGID VKNPGSNNNP 

       550        560        570 
SSNDNNSNNN DTGSEHQPVE DASLELYNIT QQILQFLH

« Hide

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References

« Hide 'large scale' references
[1]"New members of the plakoglobin/armadillo/beta-catenin family characterized by armadillo repeats."
Fischer R.
Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed: 9169868] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]"Vac8p, a vacuolar protein with armadillo repeats, functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole."
Wang Y.-X., Catlett N.L., Weisman L.S.
J. Cell Biol. 140:1063-1074(1998) [PubMed: 9490720] [Abstract]
Cited for: FUNCTION, MYRISTOYLATION AT GLY-2, PALMITOYLATION AT CYS-4; CYS-5 AND CYS-7, MUTAGENESIS OF 4-CYS--CYS-7, SUBCELLULAR LOCATION.
[4]"Apg13p and Vac8p are part of a complex of phosphoproteins that are required for cytoplasm to vacuole targeting."
Scott S.V., Nice D.C. III, Nau J.J., Weisman L.S., Kamada Y., Keizer-Gunnink I., Funakoshi T., Veenhuis M., Ohsumi Y., Klionsky D.J.
J. Biol. Chem. 275:25840-25849(2000) [PubMed: 10837477] [Abstract]
Cited for: PHOSPHORYLATION, INTERACTION WITH ATG13.
[5]"Vac8p release from the SNARE complex and its palmitoylation are coupled and essential for vacuole fusion."
Veit M., Laage R., Dietrich L., Wang L., Ungermann C.
EMBO J. 20:3145-3155(2001) [PubMed: 11406591] [Abstract]
Cited for: PALMITOYLATION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p."
Smotrys J.E., Schoenfish M.J., Stutz M.A., Linder M.E.
J. Cell Biol. 170:1091-1099(2005) [PubMed: 16186255] [Abstract]
Cited for: PALMITOYLATION.
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16, MASS SPECTROMETRY.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16 AND SER-18, MASS SPECTROMETRY.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AF005267 mRNA. Translation: AAQ13402.1.
U18530 Genomic DNA. Translation: AAB64490.1.
PIRS50446.
RefSeqNP_010903.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:5121N.
IntActP39968. 29 interactions.
STRINGP39968.

Proteomic databases

PeptideAtlasP39968.

Genome annotation databases

EnsemblYEL013W; YEL013W; YEL013W; Saccharomyces cerevisiae. [Genome view]
GeneID856702.
KEGGsce:YEL013W.
NMPDRfig|4932.3.peg.1959.

Organism-specific databases

CYGDYEL013w.
SGDS000000739. VAC8.

Phylogenomic databases

HOGENOMP39968.
OMAACIRNIS
OrthoDBEOG9R25BV

Gene expression databases

ArrayExpressP39968.
GenevestigatorP39968.
GermOnlineYEL013W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
[Graphical view]
Gene3DG3DSA:1.25.10.10. ARM-like. 2 hits.
PfamPF00514. Arm. 8 hits.
[Graphical view]
SMARTSM00185. ARM. 8 hits.
[Graphical view]
PROSITEPS50176. ARM_REPEAT. 7 hits.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio982766.

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Entry information

Entry nameVAC8_YEAST
AccessionPrimary (citable) accession number: P39968
Secondary accession number(s): Q549T0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: November 24, 2009
This is version 85 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents