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P39968

- VAC8_YEAST

UniProt

P39968 - VAC8_YEAST

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Protein

Vacuolar protein 8

Gene

VAC8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole.1 Publication

GO - Molecular functioni

  1. protein anchor Source: SGD

GO - Biological processi

  1. CVT vesicle assembly Source: SGD
  2. macroautophagy Source: SGD
  3. nucleus-vacuole junction assembly Source: SGD
  4. piecemeal microautophagy of nucleus Source: SGD
  5. vacuole fusion, non-autophagic Source: SGD
  6. vacuole inheritance Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-30139-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein 8
Gene namesi
Name:VAC8
Synonyms:YEB3
Ordered Locus Names:YEL013W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

SGDiS000000739. VAC8.

Subcellular locationi

Vacuole membrane 1 Publication; Lipid-anchor 1 Publication

GO - Cellular componenti

  1. fungal-type vacuole membrane Source: SGD
  2. Myo2p-Vac17p-Vac8p transport complex Source: SGD
  3. nucleus-vacuole junction Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi4 – 74CCSC → GTSS in VAC8-3; not palmitoylated. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 578577Vacuolar protein 8PRO_0000064302Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine1 Publication
Lipidationi4 – 41S-palmitoyl cysteine1 Publication
Lipidationi5 – 51S-palmitoyl cysteine1 Publication
Lipidationi7 – 71S-palmitoyl cysteine1 Publication
Modified residuei11 – 111Phosphoserine1 Publication
Modified residuei16 – 161Phosphoserine3 Publications

Post-translational modificationi

Palmitoylated on one or more of its N-terminal cysteine residues by PFA3, which is required for vacuole fusion.3 Publications

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiP39968.
PaxDbiP39968.
PeptideAtlasiP39968.

Expressioni

Gene expression databases

GenevestigatoriP39968.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ATG13Q066285EBI-20212,EBI-36188
NVJ1P388814EBI-20212,EBI-24885
VAB2P400032EBI-20212,EBI-22275

Protein-protein interaction databases

BioGridi36717. 105 interactions.
DIPiDIP-5121N.
IntActiP39968. 20 interactions.
MINTiMINT-517641.
STRINGi4932.YEL013W.

Structurei

3D structure databases

ProteinModelPortaliP39968.
SMRiP39968. Positions 46-518.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati37 – 7539ARM 1Add
BLAST
Repeati76 – 11439ARM 2Add
BLAST
Repeati116 – 15540ARM 3Add
BLAST
Repeati157 – 19640ARM 4Add
BLAST
Repeati198 – 23740ARM 5Add
BLAST
Repeati239 – 28042ARM 6Add
BLAST
Repeati282 – 32140ARM 7Add
BLAST
Repeati323 – 36341ARM 8Add
BLAST
Repeati407 – 44640ARM 9Add
BLAST

Sequence similaritiesi

Belongs to the beta-catenin family.Curated
Contains 9 ARM repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5064.
GeneTreeiENSGT00770000120927.
HOGENOMiHOG000177331.
InParanoidiP39968.
KOiK08332.
OMAiVQCHAIS.
OrthoDBiEOG7Q8CX4.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
[Graphical view]
PfamiPF00514. Arm. 8 hits.
[Graphical view]
SMARTiSM00185. ARM. 8 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 7 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39968-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGSCCSCLKD SSDEASVSPI ADNEREAVTL LLGYLEDKDQ LDFYSGGPLK
60 70 80 90 100
ALTTLVYSDN LNLQRSAALA FAEITEKYVR QVSREVLEPI LILLQSQDPQ
110 120 130 140 150
IQVAACAALG NLAVNNENKL LIVEMGGLEP LINQMMGDNV EVQCNAVGCI
160 170 180 190 200
TNLATRDDNK HKIATSGALI PLTKLAKSKH IRVQRNATGA LLNMTHSEEN
210 220 230 240 250
RKELVNAGAV PVLVSLLSST DPDVQYYCTT ALSNIAVDEA NRKKLAQTEP
260 270 280 290 300
RLVSKLVSLM DSPSSRVKCQ ATLALRNLAS DTSYQLEIVR AGGLPHLVKL
310 320 330 340 350
IQSDSIPLVL ASVACIRNIS IHPLNEGLIV DAGFLKPLVR LLDYKDSEEI
360 370 380 390 400
QCHAVSTLRN LAASSEKNRK EFFESGAVEK CKELALDSPV SVQSEISACF
410 420 430 440 450
AILALADVSK LDLLEANILD ALIPMTFSQN QEVSGNAAAA LANLCSRVNN
460 470 480 490 500
YTKIIEAWDR PNEGIRGFLI RFLKSDYATF EHIALWTILQ LLESHNDKVE
510 520 530 540 550
DLVKNDDDII NGVRKMADVT FERLQRSGID VKNPGSNNNP SSNDNNSNNN
560 570
DTGSEHQPVE DASLELYNIT QQILQFLH
Length:578
Mass (Da):63,208
Last modified:January 23, 2007 - v3
Checksum:iB2E75774B47E5933
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF005267 mRNA. Translation: AAQ13402.1.
U18530 Genomic DNA. Translation: AAB64490.1.
BK006939 Genomic DNA. Translation: DAA07640.1.
PIRiS50446.
RefSeqiNP_010903.3. NM_001178828.3.

Genome annotation databases

EnsemblFungiiYEL013W; YEL013W; YEL013W.
GeneIDi856702.
KEGGisce:YEL013W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF005267 mRNA. Translation: AAQ13402.1 .
U18530 Genomic DNA. Translation: AAB64490.1 .
BK006939 Genomic DNA. Translation: DAA07640.1 .
PIRi S50446.
RefSeqi NP_010903.3. NM_001178828.3.

3D structure databases

ProteinModelPortali P39968.
SMRi P39968. Positions 46-518.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36717. 105 interactions.
DIPi DIP-5121N.
IntActi P39968. 20 interactions.
MINTi MINT-517641.
STRINGi 4932.YEL013W.

Proteomic databases

MaxQBi P39968.
PaxDbi P39968.
PeptideAtlasi P39968.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YEL013W ; YEL013W ; YEL013W .
GeneIDi 856702.
KEGGi sce:YEL013W.

Organism-specific databases

SGDi S000000739. VAC8.

Phylogenomic databases

eggNOGi COG5064.
GeneTreei ENSGT00770000120927.
HOGENOMi HOG000177331.
InParanoidi P39968.
KOi K08332.
OMAi VQCHAIS.
OrthoDBi EOG7Q8CX4.

Enzyme and pathway databases

BioCyci YEAST:G3O-30139-MONOMER.

Miscellaneous databases

NextBioi 982766.

Gene expression databases

Genevestigatori P39968.

Family and domain databases

Gene3Di 1.25.10.10. 1 hit.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
[Graphical view ]
Pfami PF00514. Arm. 8 hits.
[Graphical view ]
SMARTi SM00185. ARM. 8 hits.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
PROSITEi PS50176. ARM_REPEAT. 7 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "New members of the plakoglobin/armadillo/beta-catenin family characterized by armadillo repeats."
    Fischer R.
    Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Vac8p, a vacuolar protein with armadillo repeats, functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole."
    Wang Y.-X., Catlett N.L., Weisman L.S.
    J. Cell Biol. 140:1063-1074(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MYRISTOYLATION AT GLY-2, PALMITOYLATION AT CYS-4; CYS-5 AND CYS-7, MUTAGENESIS OF 4-CYS--CYS-7, SUBCELLULAR LOCATION.
  5. "Apg13p and Vac8p are part of a complex of phosphoproteins that are required for cytoplasm to vacuole targeting."
    Scott S.V., Nice D.C. III, Nau J.J., Weisman L.S., Kamada Y., Keizer-Gunnink I., Funakoshi T., Veenhuis M., Ohsumi Y., Klionsky D.J.
    J. Biol. Chem. 275:25840-25849(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION, INTERACTION WITH ATG13.
  6. "Vac8p release from the SNARE complex and its palmitoylation are coupled and essential for vacuole fusion."
    Veit M., Laage R., Dietrich L., Wang L., Ungermann C.
    EMBO J. 20:3145-3155(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p."
    Smotrys J.E., Schoenfish M.J., Stutz M.A., Linder M.E.
    J. Cell Biol. 170:1091-1099(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION.
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11 AND SER-16, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiVAC8_YEAST
AccessioniPrimary (citable) accession number: P39968
Secondary accession number(s): D3DLN6, Q549T0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 130 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5680 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3