P39967 (UBP9_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 9
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 9
Ubiquitin thioesterase 9
Ubiquitin-specific-processing protease 9

Gene names

Name:	UBP9
Ordered Locus Names:	YER098W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	754 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Miscellaneous	Present with 98 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the peptidase C19 family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein deubiquitination Traceable author statement PubMed 8982460. Source: SGD ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred by curator PubMed 8982460. Source: SGD
Molecular_function	ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro ubiquitin-specific protease activity Traceable author statement PubMed 8982460. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 754	754	Ubiquitin carboxyl-terminal hydrolase 9		PRO_0000080594
Sites
	Active site	143	1	Nucleophile By similarity
	Active site	618	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P39967 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: D2C03D233D14D693	FASTA	754	86,244
	10 20 30 40 50 60 MIKRWLSVNR KKSHPEKNTQ GNDEINRKAT SLKKTKGSGD PSIAKSPSAK SSTSSIPSNL 70 80 90 100 110 120 ASHERRSKFS SQTDNLAGNK HYHEHYHNMA STSDEREYDS STTYEDRAFD TESSILFTTI 130 140 150 160 170 180 TDLMPYGDGS NKVFGYENFG NTCYCNSVLQ CLYNIPEFRC NVLRYPERVA AVNRIRKSDL 190 200 210 220 230 240 KGSKIRVFTN ESFETSTNSG NSNTGYQSND NEDAHNHHHL QQSDQDNSSS STQEKQNNFE 250 260 270 280 290 300 RKRNSFMGFG KDKSNYKDSA KKDDNNEMER PQPVHTVVMA SDTLTEKLHE GCKKIIVGRP 310 320 330 340 350 360 LLKQSDSLSK ASTTDCQANS HCQCDSQGSR ITSVDDDVLV NPESCNDAVN NSNNNKENTF 370 380 390 400 410 420 PTSEQRKKAA LIRGPVLNVD HLLYPTEEAT LYNGLKDIFE SITENLSLTG IVSPTEFVKI 430 440 450 460 470 480 LKKENVLFNT MMQQDAHEFL NFLLNDFSEY IQRNNPRMRF GPQKTDNSND NFITDLFKGT 490 500 510 520 530 540 LTNRIKCLTC DNITSRDEPF LDFPIEVQGD EETDIQKMLK SYHQREMLNG VNKFYCNKCY 550 560 570 580 590 600 GLQEAERMVG LKQLPHILSL HLKRFKYSEE QKSNIKLFNK ILYPLTLDVS STFNTSVYKK 610 620 630 640 650 660 YELSGVVIHM GSGPQHGHYV CICRNEKFGW LLYDDETVES IKEETVLQFT GHPGDQTTAY 670 680 690 700 710 720 VLFYKETQAD KTENQNENID TSSQDQMQTD NNIEQLIKCD DWLRDRKLRA AANIERKKTL 730 740 750 GNIPEVKTAE TKTPLNDKKR NKQKRKSRIL SFIK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18839 Genomic DNA. Translation: AAB64653.1. BK006939 Genomic DNA. Translation: DAA07759.1.
PIR	S50601.
RefSeq	NP_011024.1. NM_001178989.1.
3D structure databases
ProteinModelPortal	P39967.
SMR	P39967. Positions 383-670.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6551N.
IntAct	P39967. 1 interaction.
MINT	MINT-688224.
STRING	4932.YER098W.
Protein family/group databases
MEROPS	C19.A20.
Proteomic databases
PaxDb	P39967.
PRIDE	P39967.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER098W; YER098W; YER098W.
GeneID	856835.
KEGG	sce:YER098W.
Organism-specific databases
CYGD	YER098w.
SGD	S000000900. UBP9.
Phylogenomic databases
eggNOG	COG5077.
GeneTree	ENSGT00650000093027.
HOGENOM	HOG000141813.
KO	K11872.
OMA	NDEAINI.
OrthoDB	EOG43XZCS.
Gene expression databases
Genevestigator	P39967.
GermOnline	YER098W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	983138.

Entry information

Entry name

UBP9_YEAST

Accession

Primary (citable) accession number: P39967
Secondary accession number(s): D3DM05

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program