Protein phosphatase 2C homolog 2 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P39966 (PP2C2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 130. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein phosphatase 2C homolog 2
Short name=PP2C-2
EC=3.1.3.16

Gene names

Name:	PTC2
Ordered Locus Names:	YER089C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Responsible, together with PTC3, for the dephosphorylation of the cyclin-dependent protein kinase CDC28. Ref.4
Catalytic activity	A phosphoprotein + H₂O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Miscellaneous	Present with 12600 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the PP2C family.

Ontologies

Keywords
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA damage checkpoint Inferred from genetic interaction PubMed 10747050. Source: SGD endoplasmic reticulum unfolded protein response Inferred from physical interaction PubMed 9528768. Source: SGD inactivation of MAPK activity involved in osmosensory signaling pathway Inferred from genetic interaction PubMed 11113180 PubMed 12477803. Source: SGD protein dephosphorylation Inferred from direct assay Ref.4 PubMed 12477803. Source: SGD regulation of cyclin-dependent protein serine/threonine kinase activity Inferred from mutant phenotype Ref.4. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 14562095. Source: SGD nucleus Inferred from direct assay PubMed 14562095. Source: SGD
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PAA1	Q12447	7	EBI-12795,EBI-33397

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 464

464

Protein phosphatase 2C homolog 2

PRO_0000057775

Sites

Metal binding

Manganese 1 By similarity

Metal binding

Manganese 2 By similarity

Metal binding

Manganese 1; via carbonyl oxygen By similarity

Metal binding

234

Manganese 2 By similarity

Metal binding

283

Manganese 2 By similarity

Amino acid modifications

Modified residue

327

Phosphoserine Ref.6 Ref.7

Sequences

Sequence

Length

Mass (Da)

Tools

P39966 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 61D82D2CA4ED3FED
Show »

FASTA

464

50,388

        10         20         30         40         50         60 
MGQILSNPVI DKESHSGADS LTAFGLCAMQ GWRMSMEDSH ILEPNVLTKS DKDHIAFYGI 

        70         80         90        100        110        120 
FDGHGGAKVA EYCGNKIVEI LQEQKSFHEG NLPRALIDTF INTDVKLLQD PVMKEDHSGC 

       130        140        150        160        170        180 
TATSILVSKS QNLLVCGNAG DSRTVLATDG NAKALSYDHK PTLASEKSRI VAADGFVEMD 

       190        200        210        220        230        240 
RVNGNLALSR AIGDFEFKSN PKLGPEEQIV TCVPDILEHS LDYDRDEFVI LACDGIWDCL 

       250        260        270        280        290        300 
TSQDCVDLVH LGLREGKTLN EISSRIIDVC CAPTTEGTGI GCDNMSIVVV ALLKEGEDVA 

       310        320        330        340        350        360 
QWSDRMKSKA HRTSVRSFAD KRRRVFSYYD FSKCNDEQVF AITTKKPQDK FTRDHEAAVA 

       370        380        390        400        410        420 
SVTAADNDDP MDIDDTDADT DAENLDPSSQ SKSKTSGPID LASLEALLGA TGGVKTDSNG 

       430        440        450        460 
NKVTYTLPQS ALAQLLQTMG HDPASSHPEN DSNTDHKAGR SHLQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Maeda T., Tsai A.Y.M., Saito H. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Dephosphorylation of cyclin-dependent kinases by type 2C protein phosphatases." Cheng A., Ross K.E., Kaldis P., Solomon M.J. Genes Dev. 13:2946-2957(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-327, MASS SPECTROMETRY. Strain: YAL6B.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-327, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18839 Genomic DNA. Translation: AAB64644.1. U72498 Genomic DNA. Translation: AAB17392.1. BK006939 Genomic DNA. Translation: DAA07750.1.
PIR	S50592.
RefSeq	NP_011013.1. NM_001178980.1.
3D structure databases
ProteinModelPortal	P39966.
SMR	P39966. Positions 1-270.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1539N.
IntAct	P39966. 14 interactions.
MINT	MINT-411725.
STRING	4932.YER089C.
Proteomic databases
PaxDb	P39966.
PeptideAtlas	P39966.
PRIDE	P39966.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER089C; YER089C; YER089C.
GeneID	856823.
KEGG	sce:YER089C.
Organism-specific databases
CYGD	YER089c.
SGD	S000000891. PTC2.
Phylogenomic databases
eggNOG	COG0631.
GeneTree	ENSGT00650000093052.
HOGENOM	HOG000233896.
KO	K14803.
OMA	IGGEGCD.
OrthoDB	EOG4RFQ28.
Gene expression databases
Genevestigator	P39966.
GermOnline	YER089C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	983109.

Entry information

Entry name

PP2C2_YEAST

Accession

Primary (citable) accession number: P39966
Secondary accession number(s): D3DLZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 130 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents