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P39966

- PP2C2_YEAST

UniProt

P39966 - PP2C2_YEAST

Protein

Protein phosphatase 2C homolog 2

Gene

PTC2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 142 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Responsible, together with PTC3, for the dephosphorylation of the cyclin-dependent protein kinase CDC28.1 Publication

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi62 – 621Manganese 1By similarity
    Metal bindingi62 – 621Manganese 2By similarity
    Metal bindingi63 – 631Manganese 1; via carbonyl oxygenBy similarity
    Metal bindingi234 – 2341Manganese 2By similarity
    Metal bindingi283 – 2831Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. protein serine/threonine phosphatase activity Source: SGD

    GO - Biological processi

    1. DNA damage checkpoint Source: SGD
    2. endoplasmic reticulum unfolded protein response Source: SGD
    3. inactivation of MAPK activity involved in osmosensory signaling pathway Source: SGD
    4. protein dephosphorylation Source: SGD
    5. regulation of cyclin-dependent protein serine/threonine kinase activity Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30258-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein phosphatase 2C homolog 2 (EC:3.1.3.16)
    Short name:
    PP2C-2
    Gene namesi
    Name:PTC2
    Ordered Locus Names:YER089C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYER089c.
    SGDiS000000891. PTC2.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 464464Protein phosphatase 2C homolog 2PRO_0000057775Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei376 – 3761Phosphothreonine1 Publication
    Modified residuei380 – 3801Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP39966.
    PaxDbiP39966.
    PeptideAtlasiP39966.
    PRIDEiP39966.

    Expressioni

    Gene expression databases

    GenevestigatoriP39966.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PAA1Q124477EBI-12795,EBI-33397

    Protein-protein interaction databases

    BioGridi36834. 121 interactions.
    DIPiDIP-1539N.
    IntActiP39966. 13 interactions.
    MINTiMINT-411725.
    STRINGi4932.YER089C.

    Structurei

    3D structure databases

    ProteinModelPortaliP39966.
    SMRiP39966. Positions 1-270.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PP2C family.Curated

    Phylogenomic databases

    eggNOGiCOG0631.
    GeneTreeiENSGT00740000115384.
    HOGENOMiHOG000233896.
    KOiK14803.
    OMAiDPDINTI.
    OrthoDBiEOG79930T.

    Family and domain databases

    Gene3Di3.60.40.10. 1 hit.
    InterProiIPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view]
    PANTHERiPTHR13832. PTHR13832. 1 hit.
    PfamiPF00481. PP2C. 1 hit.
    [Graphical view]
    SMARTiSM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF81606. SSF81606. 1 hit.
    PROSITEiPS01032. PP2C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39966-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGQILSNPVI DKESHSGADS LTAFGLCAMQ GWRMSMEDSH ILEPNVLTKS    50
    DKDHIAFYGI FDGHGGAKVA EYCGNKIVEI LQEQKSFHEG NLPRALIDTF 100
    INTDVKLLQD PVMKEDHSGC TATSILVSKS QNLLVCGNAG DSRTVLATDG 150
    NAKALSYDHK PTLASEKSRI VAADGFVEMD RVNGNLALSR AIGDFEFKSN 200
    PKLGPEEQIV TCVPDILEHS LDYDRDEFVI LACDGIWDCL TSQDCVDLVH 250
    LGLREGKTLN EISSRIIDVC CAPTTEGTGI GCDNMSIVVV ALLKEGEDVA 300
    QWSDRMKSKA HRTSVRSFAD KRRRVFSYYD FSKCNDEQVF AITTKKPQDK 350
    FTRDHEAAVA SVTAADNDDP MDIDDTDADT DAENLDPSSQ SKSKTSGPID 400
    LASLEALLGA TGGVKTDSNG NKVTYTLPQS ALAQLLQTMG HDPASSHPEN 450
    DSNTDHKAGR SHLQ 464
    Length:464
    Mass (Da):50,388
    Last modified:February 1, 1995 - v1
    Checksum:i61D82D2CA4ED3FED
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18839 Genomic DNA. Translation: AAB64644.1.
    U72498 Genomic DNA. Translation: AAB17392.1.
    BK006939 Genomic DNA. Translation: DAA07750.1.
    PIRiS50592.
    RefSeqiNP_011013.1. NM_001178980.1.

    Genome annotation databases

    EnsemblFungiiYER089C; YER089C; YER089C.
    GeneIDi856823.
    KEGGisce:YER089C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18839 Genomic DNA. Translation: AAB64644.1 .
    U72498 Genomic DNA. Translation: AAB17392.1 .
    BK006939 Genomic DNA. Translation: DAA07750.1 .
    PIRi S50592.
    RefSeqi NP_011013.1. NM_001178980.1.

    3D structure databases

    ProteinModelPortali P39966.
    SMRi P39966. Positions 1-270.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36834. 121 interactions.
    DIPi DIP-1539N.
    IntActi P39966. 13 interactions.
    MINTi MINT-411725.
    STRINGi 4932.YER089C.

    Proteomic databases

    MaxQBi P39966.
    PaxDbi P39966.
    PeptideAtlasi P39966.
    PRIDEi P39966.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER089C ; YER089C ; YER089C .
    GeneIDi 856823.
    KEGGi sce:YER089C.

    Organism-specific databases

    CYGDi YER089c.
    SGDi S000000891. PTC2.

    Phylogenomic databases

    eggNOGi COG0631.
    GeneTreei ENSGT00740000115384.
    HOGENOMi HOG000233896.
    KOi K14803.
    OMAi DPDINTI.
    OrthoDBi EOG79930T.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30258-MONOMER.

    Miscellaneous databases

    NextBioi 983109.
    PROi P39966.

    Gene expression databases

    Genevestigatori P39966.

    Family and domain databases

    Gene3Di 3.60.40.10. 1 hit.
    InterProi IPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view ]
    PANTHERi PTHR13832. PTHR13832. 1 hit.
    Pfami PF00481. PP2C. 1 hit.
    [Graphical view ]
    SMARTi SM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81606. SSF81606. 1 hit.
    PROSITEi PS01032. PP2C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Maeda T., Tsai A.Y.M., Saito H.
      Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Dephosphorylation of cyclin-dependent kinases by type 2C protein phosphatases."
      Cheng A., Ross K.E., Kaldis P., Solomon M.J.
      Genes Dev. 13:2946-2957(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-376 AND THR-380, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPP2C2_YEAST
    AccessioniPrimary (citable) accession number: P39966
    Secondary accession number(s): D3DLZ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 142 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 12600 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3