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P39966 (PP2C2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 138. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein phosphatase 2C homolog 2

Short name=PP2C-2
EC=3.1.3.16
Gene names
Name:PTC2
Ordered Locus Names:YER089C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length464 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Responsible, together with PTC3, for the dephosphorylation of the cyclin-dependent protein kinase CDC28. Ref.4

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Miscellaneous

Present with 12600 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the PP2C family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PAA1Q124477EBI-12795,EBI-33397

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 464464Protein phosphatase 2C homolog 2
PRO_0000057775

Sites

Metal binding621Manganese 1 By similarity
Metal binding621Manganese 2 By similarity
Metal binding631Manganese 1; via carbonyl oxygen By similarity
Metal binding2341Manganese 2 By similarity
Metal binding2831Manganese 2 By similarity

Amino acid modifications

Modified residue3761Phosphothreonine Ref.6
Modified residue3801Phosphothreonine Ref.6

Sequences

Sequence LengthMass (Da)Tools
P39966 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 61D82D2CA4ED3FED

FASTA46450,388
        10         20         30         40         50         60 
MGQILSNPVI DKESHSGADS LTAFGLCAMQ GWRMSMEDSH ILEPNVLTKS DKDHIAFYGI 

        70         80         90        100        110        120 
FDGHGGAKVA EYCGNKIVEI LQEQKSFHEG NLPRALIDTF INTDVKLLQD PVMKEDHSGC 

       130        140        150        160        170        180 
TATSILVSKS QNLLVCGNAG DSRTVLATDG NAKALSYDHK PTLASEKSRI VAADGFVEMD 

       190        200        210        220        230        240 
RVNGNLALSR AIGDFEFKSN PKLGPEEQIV TCVPDILEHS LDYDRDEFVI LACDGIWDCL 

       250        260        270        280        290        300 
TSQDCVDLVH LGLREGKTLN EISSRIIDVC CAPTTEGTGI GCDNMSIVVV ALLKEGEDVA 

       310        320        330        340        350        360 
QWSDRMKSKA HRTSVRSFAD KRRRVFSYYD FSKCNDEQVF AITTKKPQDK FTRDHEAAVA 

       370        380        390        400        410        420 
SVTAADNDDP MDIDDTDADT DAENLDPSSQ SKSKTSGPID LASLEALLGA TGGVKTDSNG 

       430        440        450        460 
NKVTYTLPQS ALAQLLQTMG HDPASSHPEN DSNTDHKAGR SHLQ 

« Hide

References

« Hide 'large scale' references
[1]Maeda T., Tsai A.Y.M., Saito H.
Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Dephosphorylation of cyclin-dependent kinases by type 2C protein phosphatases."
Cheng A., Ross K.E., Kaldis P., Solomon M.J.
Genes Dev. 13:2946-2957(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-376 AND THR-380, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U18839 Genomic DNA. Translation: AAB64644.1.
U72498 Genomic DNA. Translation: AAB17392.1.
BK006939 Genomic DNA. Translation: DAA07750.1.
PIRS50592.
RefSeqNP_011013.1. NM_001178980.1.

3D structure databases

ProteinModelPortalP39966.
SMRP39966. Positions 1-292.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36834. 120 interactions.
DIPDIP-1539N.
IntActP39966. 13 interactions.
MINTMINT-411725.
STRING4932.YER089C.

Proteomic databases

PaxDbP39966.
PeptideAtlasP39966.
PRIDEP39966.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYER089C; YER089C; YER089C.
GeneID856823.
KEGGsce:YER089C.

Organism-specific databases

CYGDYER089c.
SGDS000000891. PTC2.

Phylogenomic databases

eggNOGCOG0631.
GeneTreeENSGT00740000115384.
HOGENOMHOG000233896.
KOK14803.
OMADPDINTI.
OrthoDBEOG79930T.

Enzyme and pathway databases

BioCycYEAST:G3O-30258-MONOMER.

Gene expression databases

GenevestigatorP39966.

Family and domain databases

Gene3D3.60.40.10. 1 hit.
InterProIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00481. PP2C. 1 hit.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. SSF81606. 1 hit.
PROSITEPS01032. PP2C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio983109.
PROP39966.

Entry information

Entry namePP2C2_YEAST
AccessionPrimary (citable) accession number: P39966
Secondary accession number(s): D3DLZ6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 19, 2014
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families