Reviewed, UniProtKB/Swiss-Prot P39965 (SYPM_YEAST)

Names and origin

Protein names

Recommended name:
    Probable prolyl-tRNA synthetase, mitochondrial
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase
      Short name=ProRS
    Altered inheritance rate of mitochondria protein 10

Gene names

Name:	AIM10
Ordered Locus Names:	YER087W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).
Subcellular location	Mitochondrion. Ref.3
Miscellaneous	Present with 477 molecules/cell in log phase SD medium. Ref.2
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Ref.3 Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 576	576	Probable prolyl-tRNA synthetase, mitochondrial		PRO_0000139354
Amino acid modifications
	Modified residue	319	1	Phosphoserine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P39965-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: D8C5BD76B47CCAF3	FASTA	576	65,880
	10 20 30 40 50 60 MLKYRTLSRS CHIFHPKSLS NNTLKSETTQ ELLQTVGFVR RSQVGLFQWL PLGLRSLNKV 70 80 90 100 110 120 SNAIRNRMDS DGGAIEVSLS AISSKALWQA TDRWNNSELF KLKDSKGKQY CLTATCEEDI 130 140 150 160 170 180 TDLMKNYIAS YKDMPITIYQ MTRKYRDEIR PRGGILRGRE FLMKDAYSFA SNEEDAFASF 190 200 210 220 230 240 QKLDDTYNKI FKDLKIPFVS AWADSGDIGG EFSKEFHLIH ESGEDTLMSC KHCGDISTLD 250 260 270 280 290 300 MSQSYPEKDG QYSGDVDCKY ALTKDHSTLI CFYYPKDRQL NWNLALNAMD KDIDLTLRNK 310 320 330 340 350 360 PNDHVLQVYE KDNEDIMFSK ILRVMDCRLN SKSNFPDFPL KKYLKNNFGQ ISDVSIVDAQ 370 380 390 400 410 420 ENEICGKCEE GRLEPLKSIE VGHIFLLGNK YSKPLNVKFV DKENKNETFV HMGCYGIGVS 430 440 450 460 470 480 RLVGAIAELG RDSNGFRWPA IMAPYKVSIC TGPNNPENSQ RLQDVKSELL NDPTMQNLQN 490 500 510 520 530 540 DILDQFNEKL GIGARIKLSH AMGIPLCVIV GSKSWPNVEI EVRGIRWGEK DLWRKQFEKR 550 560 570 CSELQWKCTK NEHGIEKHTV PIQHLAEVIG VLLKDM

References

[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[3]	"Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics." Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A. J. Proteome Res. 5:1543-1554(2006) [PubMed: 16823961] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[4]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-319, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U18839 Genomic DNA. Translation: AAB64642.1.
PIR	S50590.
RefSeq	NP_011010.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4922N.
IntAct	P39965. 4 interactions.
STRING	P39965.
Proteomic databases
PeptideAtlas	P39965.
Genome annotation databases
Ensembl	YER087W; YER087W; YER087W; Saccharomyces cerevisiae. [Genome view]
GeneID	856820.
GenomeReviews	Gene locus YER087W in contig U00092_GR.
KEGG	sce:YER087W.
NMPDR	fig|4932.3.peg.2081.
Organism-specific databases
CYGD	YER087w.
SGD	S000000889. AIM10.
Phylogenomic databases
HOGENOM	P39965.
OMA	RSNFPDF.
Enzyme and pathway databases
BRENDA	6.1.1.15. 250.
Gene expression databases
ArrayExpress	P39965.
Genevestigator	P39965.
GermOnline	YER087W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004500. Pro-tRNA-synth_IIa_bac. IPR002316. Pro-tRNA-synth_IIa_cons-reg. [Graphical view]
Pfam	PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00409. proS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	983100.

Entry information

Entry name

SYPM_YEAST

Accession

Primary (citable) accession number: P39965

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 3, 2009
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)