Pre-mRNA-splicing factor cdc5 - Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

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P39964 (CEF1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 112. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Pre-mRNA-splicing factor cdc5

Alternative name(s):
Cell division control protein 5

Gene names

Name:	cdc5
ORF Names:	SPAC644.12

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces ›

Protein attributes

Sequence length	757 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in mRNA splicing and cell cycle control. Ref.1 Ref.3
Subunit structure	Belongs to the 40S cdc5-associated complex (or cwf complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least brr2, cdc5, cwf2/prp3, cwf3/syf1, cwf4/syf3, cwf5/ecm2, spp42/cwf6, cwf7/spf27, cwf8, cwf9, cwf10, cwf11, cwf12, prp45/cwf13, cwf14, cwf15, cwf16, cwf17, cwf18, cwf19, cwf20, cwf21, cwf22, cwf23, cwf24, cwf25, cwf26, cyp7/cwf27, cwf28, cwf29/ist3, lea1, msl1, prp5/cwf1, prp10, prp12/sap130, prp17, prp22, sap61, sap62, sap114, sap145, slu7, smb1, smd1, smd3, smf1, smg1 and syf2. Ref.3 Ref.4
Subcellular location	Nucleus Ref.3.
Sequence similarities	Belongs to the CEF1 family. Contains 2 HTH myb-type DNA-binding domains.

Ontologies

Keywords
Biological process	Cell cycle mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Domain	Repeat
Ligand	DNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW mRNA cis splicing, via spliceosome Inferred from mutant phenotype Ref.3. Source: PomBase regulation of G2/M transition of mitotic cell cycle Non-traceable author statement. Source: PomBase
Cellular_component	cytosol Inferred from direct assay PubMed 20970342. Source: PomBase spliceosomal complex Inferred from direct assay PubMed 12088152. Source: PomBase
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW chromatin binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
cwf10	O94316	5	EBI-538771,EBI-538866
cwf11	O94508	3	EBI-538771,EBI-539118
cwf12	O74370	3	EBI-538771,EBI-538882
cwf17	O94620	3	EBI-538771,EBI-539124
cwf2	P87126	5	EBI-538771,EBI-538799
cwf3	Q9P7R9	7	EBI-538771,EBI-538809
cwf4	P87312	7	EBI-538771,EBI-538818
cwf5	O59800	4	EBI-538771,EBI-538826
cwf7	Q9USV3	4	EBI-538771,EBI-538841
prp45	Q09882	3	EBI-538771,EBI-457758
prp5	O13615	7	EBI-538771,EBI-538787

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 757

757

Pre-mRNA-splicing factor cdc5

PRO_0000197104

Regions

Domain

1 – 56

HTH myb-type 1

Domain

57 – 106

HTH myb-type 2

DNA binding

29 – 52

H-T-H motif By similarity

DNA binding

80 – 102

H-T-H motif By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P39964 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 9FCA639A2F9A9E84
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FASTA

757

86,844

        10         20         30         40         50         60 
MVVLKGGAWK NTEDEILKAA VSKYGKNQWA RISSLLVRKT PKQCKARWYE WIDPSIKKTE 

        70         80         90        100        110        120 
WSREEDEKLL HLAKLLPTQW RTIAPIVGRT ATQCLERYQK LLDDLEAKEN EQLGLISGEG 

       130        140        150        160        170        180 
AEAAAPVNDP NSRLRFGEAE PNLETLPALP DAIDMDEDEK EMLSEARARL ANTQGKKAKR 

       190        200        210        220        230        240 
KDREKQLELT RRLSHLQKRR ELKAAGINIK LFRRKKNEMD YNASIPFEKK PAIGFYDTSE 

       250        260        270        280        290        300 
EDRQNFREKR EADQKIIENG IRNNEMESEG RKFGHFEKPK PIDRVKKPNK DAQEEKMRRL 

       310        320        330        340        350        360 
AEAEQMSKRR KLNLPSPTVS QDELDKVVKL GFAGDRARAM TDTTPDANYS TNLLGKYTQI 

       370        380        390        400        410        420 
ERATPLRTPI SGELEGREDS VTIEVRNQLM RNREQSSLLG QESIPLQPGG TGYTGVTPSH 

       430        440        450        460        470        480 
AANGSALAAP QATPFRTPRD TFSINAAAER AGRLASEREN KIRLKALREL LAKLPKPKND 

       490        500        510        520        530        540 
YELMEPRFAD ETDVEATVGV LEEDATDRER RIQERIAEKE RLAKARRSQV IQRDLIRPSV 

       550        560        570        580        590        600 
TQPEKWKRSL ENEDPTANVL LKEMIALISS DAINYPFGNS KVKGTANKVP DLSNEEIERC 

       610        620        630        640        650        660 
RLLLKKEIGQ LESDDYIQFE KEFLETYSAL HNTSSLLPGL VIYEEDDEDV EAAEKFYTND 

       670        680        690        700        710        720 
IQRDLAKKAL ECNKLENRVY DLVRSSYEQR NFLIKKISHA WKALQTERKN LTCYEFLYNQ 

       730        740        750 
ERLALPNRLE AAEIELSKMQ QIEAYAQQDY ARVTGQN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Schizosaccharomyces pombe cdc5+ gene encodes an essential protein with homology to c-Myb." Ohi R., McCollum D., Hirani B., den Haese G.J., Zhang X., Burke J.D., Turner K., Gould K.L. EMBO J. 13:471-483(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Strain: 972 / ATCC 24843.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[3]	"Myb-related fission yeast cdc5p is a component of a 40S snRNP-containing complex and is essential for pre-mRNA splicing." McDonald W.H., Ohi R., Smelkova N., Frendewey D., Gould K.L. Mol. Cell. Biol. 19:5352-5362(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE 40S CDC5-ASSOCIATED COMPLEX.
[4]	"Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs." Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L. Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY, IDENTIFICATION IN THE CWF COMPLEX.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L19525 mRNA. Translation: AAA17515.1. CU329670 Genomic DNA. Translation: CAB90139.1.
PIR	S41712.
RefSeq	NP_593880.1. NM_001019310.2.
3D structure databases
ProteinModelPortal	P39964.
SMR	P39964. Positions 9-110.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-34817N.
IntAct	P39964. 50 interactions.
STRING	4896.SPAC644.12-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC644.12.1; SPAC644.12.1:pep; SPAC644.12.
GeneID	2543583.
KEGG	spo:SPAC644.12.
Organism-specific databases
PomBase	SPAC644.12.
Phylogenomic databases
eggNOG	COG5147.
HOGENOM	HOG000182446.
KO	K12860.
OMA	DIVKMGM.
OrthoDB	EOG48WGB3.
Family and domain databases
Gene3D	1.10.10.60. 2 hits.
InterPro	IPR021786. DUF3351. IPR009057. Homeodomain-like. IPR017930. Myb_dom. IPR001005. SANT/Myb. [Graphical view]
Pfam	PF11831. Myb_Cef. 1 hit. PF00249. Myb_DNA-binding. 2 hits. [Graphical view]
SMART	SM00717. SANT. 2 hits. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS51294. HTH_MYB. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	20804590.

Entry information

Entry name

CEF1_SCHPO

Accession

Primary (citable) accession number: P39964

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents