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P39964

- CEF1_SCHPO

UniProt

P39964 - CEF1_SCHPO

Protein

Pre-mRNA-splicing factor cdc5

Gene

cdc5

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Involved in mRNA splicing and cell cycle control.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi29 – 5224H-T-H motifPROSITE-ProRule annotationAdd
    BLAST
    DNA bindingi80 – 10223H-T-H motifPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: InterPro
    2. DNA binding Source: PomBase
    3. protein binding Source: IntAct

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. mRNA cis splicing, via spliceosome Source: PomBase

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pre-mRNA-splicing factor cdc5
    Alternative name(s):
    Cell division control protein 5
    Gene namesi
    Name:cdc5
    ORF Names:SPAC644.12
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC644.12.

    Subcellular locationi

    Nucleus 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. nucleus Source: PomBase
    2. Prp19 complex Source: PomBase
    3. spliceosomal complex Source: PomBase

    Keywords - Cellular componenti

    Nucleus, Spliceosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 757757Pre-mRNA-splicing factor cdc5PRO_0000197104Add
    BLAST

    Proteomic databases

    MaxQBiP39964.
    PRIDEiP39964.

    Interactioni

    Subunit structurei

    Belongs to the 40S cdc5-associated complex (or cwf complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least brr2, cdc5, cwf2/prp3, cwf3/syf1, cwf4/syf3, cwf5/ecm2, spp42/cwf6, cwf7/spf27, cwf8, cwf9, cwf10, cwf11, cwf12, prp45/cwf13, cwf14, cwf15, cwf16, cwf17, cwf18, cwf19, cwf20, cwf21, cwf22, cwf23, cwf24, cwf25, cwf26, cyp7/cwf27, cwf28, cwf29/ist3, lea1, msl1, prp5/cwf1, prp10, prp12/sap130, prp17, prp22, sap61, sap62, sap114, sap145, slu7, smb1, smd1, smd3, smf1, smg1 and syf2.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    cwf10O943165EBI-538771,EBI-538866
    cwf11O945083EBI-538771,EBI-539118
    cwf12O743703EBI-538771,EBI-538882
    cwf17O946203EBI-538771,EBI-539124
    cwf2P871265EBI-538771,EBI-538799
    cwf3Q9P7R97EBI-538771,EBI-538809
    cwf4P873127EBI-538771,EBI-538818
    cwf5O598004EBI-538771,EBI-538826
    cwf7Q9USV34EBI-538771,EBI-538841
    prp45Q098823EBI-538771,EBI-457758
    prp5O136157EBI-538771,EBI-538787

    Protein-protein interaction databases

    BioGridi279998. 78 interactions.
    DIPiDIP-34817N.
    IntActiP39964. 50 interactions.
    MINTiMINT-4689419.
    STRINGi4896.SPAC644.12-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP39964.
    SMRiP39964. Positions 9-110.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 5656HTH myb-type 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini57 – 10650HTH myb-type 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CEF1 family.Curated
    Contains 2 HTH myb-type DNA-binding domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5147.
    HOGENOMiHOG000182446.
    KOiK12860.
    OMAiTIDMDED.
    OrthoDBiEOG78SQWR.
    PhylomeDBiP39964.

    Family and domain databases

    Gene3Di1.10.10.60. 2 hits.
    InterProiIPR021786. DUF3351.
    IPR009057. Homeodomain-like.
    IPR017930. Myb_dom.
    IPR001005. SANT/Myb.
    [Graphical view]
    PfamiPF11831. Myb_Cef. 1 hit.
    PF00249. Myb_DNA-binding. 2 hits.
    [Graphical view]
    SMARTiSM00717. SANT. 2 hits.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 2 hits.
    PROSITEiPS51294. HTH_MYB. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39964-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVVLKGGAWK NTEDEILKAA VSKYGKNQWA RISSLLVRKT PKQCKARWYE    50
    WIDPSIKKTE WSREEDEKLL HLAKLLPTQW RTIAPIVGRT ATQCLERYQK 100
    LLDDLEAKEN EQLGLISGEG AEAAAPVNDP NSRLRFGEAE PNLETLPALP 150
    DAIDMDEDEK EMLSEARARL ANTQGKKAKR KDREKQLELT RRLSHLQKRR 200
    ELKAAGINIK LFRRKKNEMD YNASIPFEKK PAIGFYDTSE EDRQNFREKR 250
    EADQKIIENG IRNNEMESEG RKFGHFEKPK PIDRVKKPNK DAQEEKMRRL 300
    AEAEQMSKRR KLNLPSPTVS QDELDKVVKL GFAGDRARAM TDTTPDANYS 350
    TNLLGKYTQI ERATPLRTPI SGELEGREDS VTIEVRNQLM RNREQSSLLG 400
    QESIPLQPGG TGYTGVTPSH AANGSALAAP QATPFRTPRD TFSINAAAER 450
    AGRLASEREN KIRLKALREL LAKLPKPKND YELMEPRFAD ETDVEATVGV 500
    LEEDATDRER RIQERIAEKE RLAKARRSQV IQRDLIRPSV TQPEKWKRSL 550
    ENEDPTANVL LKEMIALISS DAINYPFGNS KVKGTANKVP DLSNEEIERC 600
    RLLLKKEIGQ LESDDYIQFE KEFLETYSAL HNTSSLLPGL VIYEEDDEDV 650
    EAAEKFYTND IQRDLAKKAL ECNKLENRVY DLVRSSYEQR NFLIKKISHA 700
    WKALQTERKN LTCYEFLYNQ ERLALPNRLE AAEIELSKMQ QIEAYAQQDY 750
    ARVTGQN 757
    Length:757
    Mass (Da):86,844
    Last modified:February 1, 1995 - v1
    Checksum:i9FCA639A2F9A9E84
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19525 mRNA. Translation: AAA17515.1.
    CU329670 Genomic DNA. Translation: CAB90139.1.
    PIRiS41712.
    RefSeqiNP_593880.1. NM_001019310.2.

    Genome annotation databases

    EnsemblFungiiSPAC644.12.1; SPAC644.12.1:pep; SPAC644.12.
    GeneIDi2543583.
    KEGGispo:SPAC644.12.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19525 mRNA. Translation: AAA17515.1 .
    CU329670 Genomic DNA. Translation: CAB90139.1 .
    PIRi S41712.
    RefSeqi NP_593880.1. NM_001019310.2.

    3D structure databases

    ProteinModelPortali P39964.
    SMRi P39964. Positions 9-110.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 279998. 78 interactions.
    DIPi DIP-34817N.
    IntActi P39964. 50 interactions.
    MINTi MINT-4689419.
    STRINGi 4896.SPAC644.12-1.

    Proteomic databases

    MaxQBi P39964.
    PRIDEi P39964.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC644.12.1 ; SPAC644.12.1:pep ; SPAC644.12 .
    GeneIDi 2543583.
    KEGGi spo:SPAC644.12.

    Organism-specific databases

    PomBasei SPAC644.12.

    Phylogenomic databases

    eggNOGi COG5147.
    HOGENOMi HOG000182446.
    KOi K12860.
    OMAi TIDMDED.
    OrthoDBi EOG78SQWR.
    PhylomeDBi P39964.

    Miscellaneous databases

    NextBioi 20804590.
    PROi P39964.

    Family and domain databases

    Gene3Di 1.10.10.60. 2 hits.
    InterProi IPR021786. DUF3351.
    IPR009057. Homeodomain-like.
    IPR017930. Myb_dom.
    IPR001005. SANT/Myb.
    [Graphical view ]
    Pfami PF11831. Myb_Cef. 1 hit.
    PF00249. Myb_DNA-binding. 2 hits.
    [Graphical view ]
    SMARTi SM00717. SANT. 2 hits.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 2 hits.
    PROSITEi PS51294. HTH_MYB. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Schizosaccharomyces pombe cdc5+ gene encodes an essential protein with homology to c-Myb."
      Ohi R., McCollum D., Hirani B., den Haese G.J., Zhang X., Burke J.D., Turner K., Gould K.L.
      EMBO J. 13:471-483(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
      Strain: 972 / ATCC 24843.
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. "Myb-related fission yeast cdc5p is a component of a 40S snRNP-containing complex and is essential for pre-mRNA splicing."
      McDonald W.H., Ohi R., Smelkova N., Frendewey D., Gould K.L.
      Mol. Cell. Biol. 19:5352-5362(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE 40S CDC5-ASSOCIATED COMPLEX.
    4. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
      Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
      Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CWF COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiCEF1_SCHPO
    AccessioniPrimary (citable) accession number: P39964
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3