Casein kinase I homolog 3 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P39962 (KC13_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 112. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Casein kinase I homolog 3
EC=2.7.11.1

Gene names

Name:	YCK3
Synonyms:	CKI3
Ordered Locus Names:	YER123W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side. Nucleus membrane; Lipid-anchor; Cytoplasmic side. Vacuole membrane; Lipid-anchor; Cytoplasmic side. Note: Targeting to the vacuolar membrane may depend on AP-3 pathway.
Domain	The YXXZ (Tyr-Xaa-Xaa-Zaa, where Zaa is a hydrophobic residue) motif mediates the targeting to the lysosomal compartments.
Miscellaneous	Present with 1320 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Nucleus Vacuole
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	vesicle-mediated transport Inferred from mutant phenotype. Source: SGD
Cellular component	fungal-type vacuole membrane Inferred from direct assay Ref.5. Source: SGD nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay Ref.1. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 524

524

Casein kinase I homolog 3

PRO_0000192858

Regions

Domain

14 – 319

306

Protein kinase

Nucleotide binding

20 – 28

ATP By similarity

Motif

444 – 447

YXXZ targeting signal

Sites

Active site

150

Proton acceptor By similarity

Binding site

ATP By similarity

Amino acid modifications

Modified residue

408

Phosphoserine Ref.7

Modified residue

431

Phosphoserine Ref.6

Lipidation

517

S-palmitoyl cysteine Probable

Lipidation

518

S-palmitoyl cysteine Probable

Lipidation

519

S-palmitoyl cysteine Probable

Lipidation

520

S-palmitoyl cysteine Probable

Lipidation

522

S-palmitoyl cysteine Probable

Lipidation

523

S-palmitoyl cysteine Probable

Lipidation

524

S-palmitoyl cysteine Probable

Experimental info

Mutagenesis

444

Y → H: Impaired vacuolar targeting. Ref.5

Mutagenesis

446

S → P: Impaired vacuolar targeting. Ref.5

Mutagenesis

447

I → N: Impaired vacuolar targeting. Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

P39962 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: FD4BBA311D0CD805
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FASTA

524

60,261

        10         20         30         40         50         60 
MSQRSSQHIV GIHYAVGPKI GEGSFGVIFE GENILHSCQA QTGSKRDSSI IMANEPVAIK 

        70         80         90        100        110        120 
FEPRHSDAPQ LRDEFRAYRI LNGCVGIPHA YYFGQEGMHN ILIIDLLGPS LEDLFEWCGR 

       130        140        150        160        170        180 
KFSVKTTCMV AKQMIDRVRA IHDHDLIYRD IKPDNFLISQ YQRISPEGKV IKSCASSSNN 

       190        200        210        220        230        240 
DPNLIYMVDF GMAKQYRDPR TKQHIPYRER KSLSGTARYM SINTHFGREQ SRRDDLESLG 

       250        260        270        280        290        300 
HVFFYFLRGS LPWQGLKAPN NKLKYEKIGM TKQKLNPDDL LLNNAIPYQF ATYLKYARSL 

       310        320        330        340        350        360 
KFDEDPDYDY LISLMDDALR LNDLKDDGHY DWMDLNGGKG WNIKINRRAN LHGYGNPNPR 

       370        380        390        400        410        420 
VNGNTARNNV NTNSKTRNTT PVATPKQQAQ NSYNKDNSKS RISSNPQSFT KQQHVLKKIE 

       430        440        450        460        470        480 
PNSKYIPETH SNLQRPIKSQ SQTYDSISHT QNSPFVPYSS SKANPKRSNN EHNLPNHYTN 

       490        500        510        520 
LANKNINYQS QRNYEQENDA YSDDENDTFC SKIYKYCCCC FCCC

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prenylated isoforms of yeast casein kinase I, including the novel Yck3p, suppress the gcs1 blockage of cell proliferation from stationary phase." Wang X., Hoekstra M.F., Demaggio A.J., Dhillon N., Vancura A., Kuret J., Johnston G.C., Singer R.A. Mol. Cell. Biol. 16:5375-5385(1996) [PubMed: 8816449] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"The yeast casein kinase Yck3p is palmitoylated, then sorted to the vacuolar membrane with AP-3-dependent recognition of a YXXPhi adaptin sorting signal." Sun B., Chen L., Cao W., Roth A.F., Davis N.G. Mol. Biol. Cell 15:1397-1406(2004) [PubMed: 14668479] [Abstract] Cited for: PALMITOYLATION AT CYS-517; CYS-518; CYS-519; CYS-520; CYS-522; CYS-523 AND CYS-524, TARGETING SIGNAL, MUTAGENESIS OF TYR-444; SER-446 AND ILE-447.
[6]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-431, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-408, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X87108 Genomic DNA. Translation: CAA60589.1. U18916 Genomic DNA. Translation: AAC03221.2. BK006939 Genomic DNA. Translation: DAA07783.1.
PIR	S50626.
RefSeq	NP_011049.2. NM_001179013.1.
3D structure databases
ProteinModelPortal	P39962.
SMR	P39962. Positions 8-332.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6331N.
IntAct	P39962. 6 interactions.
STRING	P39962.
Proteomic databases
PeptideAtlas	P39962.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER123W; YER123W; YER123W.
GeneID	856860.
KEGG	sce:YER123W.
NMPDR	fig\|4932.3.peg.2125.
Organism-specific databases
CYGD	YER123w.
SGD	S000000925. YCK3.
Phylogenomic databases
eggNOG	fuNOG04993.
GeneTree	EFGT00050000000354.
HOGENOM	HBG755340.
OMA	RANLHGY.
OrthoDB	EOG4D29ZN.
Enzyme and pathway databases
BRENDA	2.7.11.1. 984.
Gene expression databases
ArrayExpress	P39962.
Genevestigator	P39962.
GermOnline	YER123W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
KO	K02218.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	983210.

Entry information

Entry name

KC13_YEAST

Accession

Primary (citable) accession number: P39962
Secondary accession number(s): D3DM29

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 14, 2011
This is version 112 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents