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P39962

- KC13_YEAST

UniProt

P39962 - KC13_YEAST

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Protein

Casein kinase I homolog 3

Gene
YCK3, CKI3, YER123W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei60 – 601ATP By similarity
Active sitei150 – 1501Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi20 – 289ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  1. protein phosphorylation Source: SGD
  2. vesicle-mediated transport Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YER123W-MONOMER.
BRENDAi2.7.11.1. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Casein kinase I homolog 3 (EC:2.7.11.1)
Gene namesi
Name:YCK3
Synonyms:CKI3
Ordered Locus Names:YER123W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER123w.
SGDiS000000925. YCK3.

Subcellular locationi

GO - Cellular componenti

  1. fungal-type vacuole Source: SGD
  2. fungal-type vacuole membrane Source: SGD
  3. nuclear membrane Source: UniProtKB-SubCell
  4. nucleus Source: SGD
  5. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus, Vacuole

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi444 – 4441Y → H: Impaired vacuolar targeting. 1 Publication
Mutagenesisi446 – 4461S → P: Impaired vacuolar targeting. 1 Publication
Mutagenesisi447 – 4471I → N: Impaired vacuolar targeting. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 524524Casein kinase I homolog 3PRO_0000192858Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi517 – 5171S-palmitoyl cysteine Inferred
Lipidationi518 – 5181S-palmitoyl cysteine Inferred
Lipidationi519 – 5191S-palmitoyl cysteine Inferred
Lipidationi520 – 5201S-palmitoyl cysteine Inferred
Lipidationi522 – 5221S-palmitoyl cysteine Inferred
Lipidationi523 – 5231S-palmitoyl cysteine Inferred
Lipidationi524 – 5241S-palmitoyl cysteine Inferred

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

MaxQBiP39962.
PaxDbiP39962.
PeptideAtlasiP39962.

Expressioni

Gene expression databases

GenevestigatoriP39962.

Interactioni

Protein-protein interaction databases

BioGridi36867. 93 interactions.
DIPiDIP-6331N.
IntActiP39962. 4 interactions.
MINTiMINT-4484355.
STRINGi4932.YER123W.

Structurei

3D structure databases

ProteinModelPortaliP39962.
SMRiP39962. Positions 8-332.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 319306Protein kinaseAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi444 – 4474YXXZ targeting signal

Domaini

The YXXZ (Tyr-Xaa-Xaa-Zaa, where Zaa is a hydrophobic residue) motif mediates the targeting to the lysosomal compartments.

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG238400.
GeneTreeiENSGT00750000118039.
HOGENOMiHOG000182055.
KOiK02218.
OMAiRANLHGY.
OrthoDBiEOG7WQ832.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39962-1 [UniParc]FASTAAdd to Basket

« Hide

MSQRSSQHIV GIHYAVGPKI GEGSFGVIFE GENILHSCQA QTGSKRDSSI    50
IMANEPVAIK FEPRHSDAPQ LRDEFRAYRI LNGCVGIPHA YYFGQEGMHN 100
ILIIDLLGPS LEDLFEWCGR KFSVKTTCMV AKQMIDRVRA IHDHDLIYRD 150
IKPDNFLISQ YQRISPEGKV IKSCASSSNN DPNLIYMVDF GMAKQYRDPR 200
TKQHIPYRER KSLSGTARYM SINTHFGREQ SRRDDLESLG HVFFYFLRGS 250
LPWQGLKAPN NKLKYEKIGM TKQKLNPDDL LLNNAIPYQF ATYLKYARSL 300
KFDEDPDYDY LISLMDDALR LNDLKDDGHY DWMDLNGGKG WNIKINRRAN 350
LHGYGNPNPR VNGNTARNNV NTNSKTRNTT PVATPKQQAQ NSYNKDNSKS 400
RISSNPQSFT KQQHVLKKIE PNSKYIPETH SNLQRPIKSQ SQTYDSISHT 450
QNSPFVPYSS SKANPKRSNN EHNLPNHYTN LANKNINYQS QRNYEQENDA 500
YSDDENDTFC SKIYKYCCCC FCCC 524
Length:524
Mass (Da):60,261
Last modified:November 1, 1995 - v2
Checksum:iFD4BBA311D0CD805
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X87108 Genomic DNA. Translation: CAA60589.1.
U18916 Genomic DNA. Translation: AAC03221.2.
BK006939 Genomic DNA. Translation: DAA07783.1.
PIRiS50626.
RefSeqiNP_011049.2. NM_001179013.1.

Genome annotation databases

EnsemblFungiiYER123W; YER123W; YER123W.
GeneIDi856860.
KEGGisce:YER123W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X87108 Genomic DNA. Translation: CAA60589.1 .
U18916 Genomic DNA. Translation: AAC03221.2 .
BK006939 Genomic DNA. Translation: DAA07783.1 .
PIRi S50626.
RefSeqi NP_011049.2. NM_001179013.1.

3D structure databases

ProteinModelPortali P39962.
SMRi P39962. Positions 8-332.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36867. 93 interactions.
DIPi DIP-6331N.
IntActi P39962. 4 interactions.
MINTi MINT-4484355.
STRINGi 4932.YER123W.

Proteomic databases

MaxQBi P39962.
PaxDbi P39962.
PeptideAtlasi P39962.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER123W ; YER123W ; YER123W .
GeneIDi 856860.
KEGGi sce:YER123W.

Organism-specific databases

CYGDi YER123w.
SGDi S000000925. YCK3.

Phylogenomic databases

eggNOGi NOG238400.
GeneTreei ENSGT00750000118039.
HOGENOMi HOG000182055.
KOi K02218.
OMAi RANLHGY.
OrthoDBi EOG7WQ832.

Enzyme and pathway databases

BioCyci YEAST:YER123W-MONOMER.
BRENDAi 2.7.11.1. 984.

Miscellaneous databases

NextBioi 983210.

Gene expression databases

Genevestigatori P39962.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Prenylated isoforms of yeast casein kinase I, including the novel Yck3p, suppress the gcs1 blockage of cell proliferation from stationary phase."
    Wang X., Hoekstra M.F., Demaggio A.J., Dhillon N., Vancura A., Kuret J., Johnston G.C., Singer R.A.
    Mol. Cell. Biol. 16:5375-5385(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "The yeast casein kinase Yck3p is palmitoylated, then sorted to the vacuolar membrane with AP-3-dependent recognition of a YXXPhi adaptin sorting signal."
    Sun B., Chen L., Cao W., Roth A.F., Davis N.G.
    Mol. Biol. Cell 15:1397-1406(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION AT CYS-517; CYS-518; CYS-519; CYS-520; CYS-522; CYS-523 AND CYS-524, TARGETING SIGNAL, MUTAGENESIS OF TYR-444; SER-446 AND ILE-447.
  6. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKC13_YEAST
AccessioniPrimary (citable) accession number: P39962
Secondary accession number(s): D3DM29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1995
Last modified: May 14, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1320 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

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