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P39962 (KC13_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Casein kinase I homolog 3

EC=2.7.11.1
Gene names
Name:YCK3
Synonyms:CKI3
Ordered Locus Names:YER123W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length524 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic side. Nucleus membrane; Lipid-anchor; Cytoplasmic side. Vacuole membrane; Lipid-anchor; Cytoplasmic side. Note: Targeting to the vacuolar membrane may depend on AP-3 pathway.

Domain

The YXXZ (Tyr-Xaa-Xaa-Zaa, where Zaa is a hydrophobic residue) motif mediates the targeting to the lysosomal compartments.

Miscellaneous

Present with 1320 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 524524Casein kinase I homolog 3
PRO_0000192858

Regions

Domain14 – 319306Protein kinase
Nucleotide binding20 – 289ATP By similarity
Motif444 – 4474YXXZ targeting signal

Sites

Active site1501Proton acceptor By similarity
Binding site601ATP By similarity

Amino acid modifications

Lipidation5171S-palmitoyl cysteine Probable
Lipidation5181S-palmitoyl cysteine Probable
Lipidation5191S-palmitoyl cysteine Probable
Lipidation5201S-palmitoyl cysteine Probable
Lipidation5221S-palmitoyl cysteine Probable
Lipidation5231S-palmitoyl cysteine Probable
Lipidation5241S-palmitoyl cysteine Probable

Experimental info

Mutagenesis4441Y → H: Impaired vacuolar targeting. Ref.5
Mutagenesis4461S → P: Impaired vacuolar targeting. Ref.5
Mutagenesis4471I → N: Impaired vacuolar targeting. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P39962 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: FD4BBA311D0CD805

FASTA52460,261
        10         20         30         40         50         60 
MSQRSSQHIV GIHYAVGPKI GEGSFGVIFE GENILHSCQA QTGSKRDSSI IMANEPVAIK 

        70         80         90        100        110        120 
FEPRHSDAPQ LRDEFRAYRI LNGCVGIPHA YYFGQEGMHN ILIIDLLGPS LEDLFEWCGR 

       130        140        150        160        170        180 
KFSVKTTCMV AKQMIDRVRA IHDHDLIYRD IKPDNFLISQ YQRISPEGKV IKSCASSSNN 

       190        200        210        220        230        240 
DPNLIYMVDF GMAKQYRDPR TKQHIPYRER KSLSGTARYM SINTHFGREQ SRRDDLESLG 

       250        260        270        280        290        300 
HVFFYFLRGS LPWQGLKAPN NKLKYEKIGM TKQKLNPDDL LLNNAIPYQF ATYLKYARSL 

       310        320        330        340        350        360 
KFDEDPDYDY LISLMDDALR LNDLKDDGHY DWMDLNGGKG WNIKINRRAN LHGYGNPNPR 

       370        380        390        400        410        420 
VNGNTARNNV NTNSKTRNTT PVATPKQQAQ NSYNKDNSKS RISSNPQSFT KQQHVLKKIE 

       430        440        450        460        470        480 
PNSKYIPETH SNLQRPIKSQ SQTYDSISHT QNSPFVPYSS SKANPKRSNN EHNLPNHYTN 

       490        500        510        520 
LANKNINYQS QRNYEQENDA YSDDENDTFC SKIYKYCCCC FCCC 

« Hide

References

« Hide 'large scale' references
[1]"Prenylated isoforms of yeast casein kinase I, including the novel Yck3p, suppress the gcs1 blockage of cell proliferation from stationary phase."
Wang X., Hoekstra M.F., Demaggio A.J., Dhillon N., Vancura A., Kuret J., Johnston G.C., Singer R.A.
Mol. Cell. Biol. 16:5375-5385(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"The yeast casein kinase Yck3p is palmitoylated, then sorted to the vacuolar membrane with AP-3-dependent recognition of a YXXPhi adaptin sorting signal."
Sun B., Chen L., Cao W., Roth A.F., Davis N.G.
Mol. Biol. Cell 15:1397-1406(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PALMITOYLATION AT CYS-517; CYS-518; CYS-519; CYS-520; CYS-522; CYS-523 AND CYS-524, TARGETING SIGNAL, MUTAGENESIS OF TYR-444; SER-446 AND ILE-447.
[6]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X87108 Genomic DNA. Translation: CAA60589.1.
U18916 Genomic DNA. Translation: AAC03221.2.
BK006939 Genomic DNA. Translation: DAA07783.1.
PIRS50626.
RefSeqNP_011049.2. NM_001179013.1.

3D structure databases

ProteinModelPortalP39962.
SMRP39962. Positions 8-332.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36867. 93 interactions.
DIPDIP-6331N.
IntActP39962. 4 interactions.
MINTMINT-4484355.
STRING4932.YER123W.

Proteomic databases

MaxQBP39962.
PaxDbP39962.
PeptideAtlasP39962.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYER123W; YER123W; YER123W.
GeneID856860.
KEGGsce:YER123W.

Organism-specific databases

CYGDYER123w.
SGDS000000925. YCK3.

Phylogenomic databases

eggNOGNOG238400.
GeneTreeENSGT00750000118039.
HOGENOMHOG000182055.
KOK02218.
OMARANLHGY.
OrthoDBEOG7WQ832.

Enzyme and pathway databases

BioCycYEAST:YER123W-MONOMER.
BRENDA2.7.11.1. 984.

Gene expression databases

GenevestigatorP39962.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio983210.

Entry information

Entry nameKC13_YEAST
AccessionPrimary (citable) accession number: P39962
Secondary accession number(s): D3DM29
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1995
Last modified: May 14, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families