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P39962

- KC13_YEAST

UniProt

P39962 - KC13_YEAST

Protein

Casein kinase I homolog 3

Gene

YCK3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 2 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates.

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei60 – 601ATPPROSITE-ProRule annotation
    Active sitei150 – 1501Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi20 – 289ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein serine/threonine kinase activity Source: SGD

    GO - Biological processi

    1. protein phosphorylation Source: SGD
    2. vesicle-mediated transport Source: SGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:YER123W-MONOMER.
    BRENDAi2.7.11.1. 984.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Casein kinase I homolog 3 (EC:2.7.11.1)
    Gene namesi
    Name:YCK3
    Synonyms:CKI3
    Ordered Locus Names:YER123W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYER123w.
    SGDiS000000925. YCK3.

    Subcellular locationi

    GO - Cellular componenti

    1. fungal-type vacuole Source: SGD
    2. fungal-type vacuole membrane Source: SGD
    3. nuclear membrane Source: UniProtKB-SubCell
    4. nucleus Source: SGD
    5. plasma membrane Source: SGD

    Keywords - Cellular componenti

    Cell membrane, Membrane, Nucleus, Vacuole

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi444 – 4441Y → H: Impaired vacuolar targeting. 1 Publication
    Mutagenesisi446 – 4461S → P: Impaired vacuolar targeting. 1 Publication
    Mutagenesisi447 – 4471I → N: Impaired vacuolar targeting. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 524524Casein kinase I homolog 3PRO_0000192858Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi517 – 5171S-palmitoyl cysteine1 Publication
    Lipidationi518 – 5181S-palmitoyl cysteine1 Publication
    Lipidationi519 – 5191S-palmitoyl cysteine1 Publication
    Lipidationi520 – 5201S-palmitoyl cysteine1 Publication
    Lipidationi522 – 5221S-palmitoyl cysteine1 Publication
    Lipidationi523 – 5231S-palmitoyl cysteine1 Publication
    Lipidationi524 – 5241S-palmitoyl cysteine1 Publication

    Keywords - PTMi

    Lipoprotein, Palmitate

    Proteomic databases

    MaxQBiP39962.
    PaxDbiP39962.
    PeptideAtlasiP39962.

    Expressioni

    Gene expression databases

    GenevestigatoriP39962.

    Interactioni

    Protein-protein interaction databases

    BioGridi36867. 93 interactions.
    DIPiDIP-6331N.
    IntActiP39962. 4 interactions.
    MINTiMINT-4484355.
    STRINGi4932.YER123W.

    Structurei

    3D structure databases

    ProteinModelPortaliP39962.
    SMRiP39962. Positions 8-332.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini14 – 319306Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi444 – 4474YXXZ targeting signal

    Domaini

    The YXXZ (Tyr-Xaa-Xaa-Zaa, where Zaa is a hydrophobic residue) motif mediates the targeting to the lysosomal compartments.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG238400.
    GeneTreeiENSGT00750000118039.
    HOGENOMiHOG000182055.
    KOiK02218.
    OMAiRANLHGY.
    OrthoDBiEOG7WQ832.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39962-1 [UniParc]FASTAAdd to Basket

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    MSQRSSQHIV GIHYAVGPKI GEGSFGVIFE GENILHSCQA QTGSKRDSSI    50
    IMANEPVAIK FEPRHSDAPQ LRDEFRAYRI LNGCVGIPHA YYFGQEGMHN 100
    ILIIDLLGPS LEDLFEWCGR KFSVKTTCMV AKQMIDRVRA IHDHDLIYRD 150
    IKPDNFLISQ YQRISPEGKV IKSCASSSNN DPNLIYMVDF GMAKQYRDPR 200
    TKQHIPYRER KSLSGTARYM SINTHFGREQ SRRDDLESLG HVFFYFLRGS 250
    LPWQGLKAPN NKLKYEKIGM TKQKLNPDDL LLNNAIPYQF ATYLKYARSL 300
    KFDEDPDYDY LISLMDDALR LNDLKDDGHY DWMDLNGGKG WNIKINRRAN 350
    LHGYGNPNPR VNGNTARNNV NTNSKTRNTT PVATPKQQAQ NSYNKDNSKS 400
    RISSNPQSFT KQQHVLKKIE PNSKYIPETH SNLQRPIKSQ SQTYDSISHT 450
    QNSPFVPYSS SKANPKRSNN EHNLPNHYTN LANKNINYQS QRNYEQENDA 500
    YSDDENDTFC SKIYKYCCCC FCCC 524
    Length:524
    Mass (Da):60,261
    Last modified:November 1, 1995 - v2
    Checksum:iFD4BBA311D0CD805
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X87108 Genomic DNA. Translation: CAA60589.1.
    U18916 Genomic DNA. Translation: AAC03221.2.
    BK006939 Genomic DNA. Translation: DAA07783.1.
    PIRiS50626.
    RefSeqiNP_011049.2. NM_001179013.1.

    Genome annotation databases

    EnsemblFungiiYER123W; YER123W; YER123W.
    GeneIDi856860.
    KEGGisce:YER123W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X87108 Genomic DNA. Translation: CAA60589.1 .
    U18916 Genomic DNA. Translation: AAC03221.2 .
    BK006939 Genomic DNA. Translation: DAA07783.1 .
    PIRi S50626.
    RefSeqi NP_011049.2. NM_001179013.1.

    3D structure databases

    ProteinModelPortali P39962.
    SMRi P39962. Positions 8-332.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36867. 93 interactions.
    DIPi DIP-6331N.
    IntActi P39962. 4 interactions.
    MINTi MINT-4484355.
    STRINGi 4932.YER123W.

    Proteomic databases

    MaxQBi P39962.
    PaxDbi P39962.
    PeptideAtlasi P39962.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER123W ; YER123W ; YER123W .
    GeneIDi 856860.
    KEGGi sce:YER123W.

    Organism-specific databases

    CYGDi YER123w.
    SGDi S000000925. YCK3.

    Phylogenomic databases

    eggNOGi NOG238400.
    GeneTreei ENSGT00750000118039.
    HOGENOMi HOG000182055.
    KOi K02218.
    OMAi RANLHGY.
    OrthoDBi EOG7WQ832.

    Enzyme and pathway databases

    BioCyci YEAST:YER123W-MONOMER.
    BRENDAi 2.7.11.1. 984.

    Miscellaneous databases

    NextBioi 983210.

    Gene expression databases

    Genevestigatori P39962.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Prenylated isoforms of yeast casein kinase I, including the novel Yck3p, suppress the gcs1 blockage of cell proliferation from stationary phase."
      Wang X., Hoekstra M.F., Demaggio A.J., Dhillon N., Vancura A., Kuret J., Johnston G.C., Singer R.A.
      Mol. Cell. Biol. 16:5375-5385(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "The yeast casein kinase Yck3p is palmitoylated, then sorted to the vacuolar membrane with AP-3-dependent recognition of a YXXPhi adaptin sorting signal."
      Sun B., Chen L., Cao W., Roth A.F., Davis N.G.
      Mol. Biol. Cell 15:1397-1406(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PALMITOYLATION AT CYS-517; CYS-518; CYS-519; CYS-520; CYS-522; CYS-523 AND CYS-524, TARGETING SIGNAL, MUTAGENESIS OF TYR-444; SER-446 AND ILE-447.
    6. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiKC13_YEAST
    AccessioniPrimary (citable) accession number: P39962
    Secondary accession number(s): D3DM29
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 133 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1320 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3