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Protein

GTPase-activating protein BEM2/IPL2

Gene

BEM2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTPase-activating protein (GAP) for RHO1 and RHO2. Involved in the control of cellular morphogenesis. Required for proper bud site selection and bud emergence.

Miscellaneous

Present with 1230 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • GTPase activator activity Source: SGD
  • guanyl-nucleotide exchange factor activity Source: InterPro

GO - Biological processi

  • actin cytoskeleton organization Source: SGD
  • establishment of cell polarity Source: SGD
  • negative regulation of Rho protein signal transduction Source: SGD
  • small GTPase mediated signal transduction Source: InterPro

Keywordsi

Molecular functionGTPase activation

Enzyme and pathway databases

BioCyciYEAST:G3O-30316-MONOMER
ReactomeiR-SCE-193648 NRAGE signals death through JNK
R-SCE-194840 Rho GTPase cycle
R-SCE-416482 G alpha (12/13) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase-activating protein BEM2/IPL2
Alternative name(s):
Bud emergence protein 2
Gene namesi
Name:BEM2
Synonyms:IPL2, SUP9
Ordered Locus Names:YER155C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER155C
SGDiS000000957 BEM2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000688581 – 2167GTPase-activating protein BEM2/IPL2Add BLAST2167

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki27Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei129PhosphoserineCombined sources1
Modified residuei283PhosphoserineCombined sources1
Modified residuei1012PhosphoserineCombined sources1
Modified residuei1016PhosphoserineCombined sources1
Modified residuei1038PhosphothreonineCombined sources1
Modified residuei1046PhosphoserineCombined sources1
Modified residuei1054PhosphoserineCombined sources1
Modified residuei1128PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP39960
PaxDbiP39960
PRIDEiP39960

PTM databases

iPTMnetiP39960

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
BUD14P276373EBI-3517,EBI-20747

GO - Molecular functioni

Protein-protein interaction databases

BioGridi36905, 541 interactors
DIPiDIP-6580N
IntActiP39960, 48 interactors
MINTiP39960
STRINGi4932.YER155C

Structurei

3D structure databases

ProteinModelPortaliP39960
SMRiP39960
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini592 – 859Ras-GEFPROSITE-ProRule annotationAdd BLAST268
Domaini1846 – 1948PHPROSITE-ProRule annotationAdd BLAST103
Domaini1967 – 2165Rho-GAPPROSITE-ProRule annotationAdd BLAST199

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi16 – 25Poly-Ser10
Compositional biasi35 – 43Poly-Ser9
Compositional biasi58 – 63Poly-His6
Compositional biasi198 – 208Poly-AsnAdd BLAST11
Compositional biasi253 – 260Poly-Ser8
Compositional biasi1161 – 1165Poly-Thr5

Phylogenomic databases

GeneTreeiENSGT00910000143995
HOGENOMiHOG000095195
InParanoidiP39960
KOiK19844
OMAiCFGWIIE
OrthoDBiEOG092C047S

Family and domain databases

CDDicd00155 RasGEF, 1 hit
cd06224 REM, 1 hit
Gene3Di1.10.555.10, 1 hit
1.10.840.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR000651 Ras-like_Gua-exchang_fac_N
IPR023578 Ras_GEF_dom_sf
IPR001895 RASGEF_cat_dom
IPR036964 RASGEF_cat_dom_sf
IPR008936 Rho_GTPase_activation_prot
IPR000198 RhoGAP_dom
PfamiView protein in Pfam
PF00169 PH, 1 hit
PF00617 RasGEF, 1 hit
PF00618 RasGEF_N, 1 hit
PF00620 RhoGAP, 1 hit
SMARTiView protein in SMART
SM00147 RasGEF, 1 hit
SM00229 RasGEFN, 1 hit
SM00324 RhoGAP, 1 hit
SUPFAMiSSF48350 SSF48350, 1 hit
SSF48366 SSF48366, 5 hits
PROSITEiView protein in PROSITE
PS50003 PH_DOMAIN, 1 hit
PS50009 RASGEF_CAT, 1 hit
PS50238 RHOGAP, 1 hit

Sequencei

Sequence statusi: Complete.

P39960-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGLLWSKNR KSSTASASSS STSTSHKTTT ASTASSSSPS SSSQTIRNST
60 70 80 90 100
SGASPYMHSH HHHGQGHSHH RGEDNNRDKR KSSVFPPSKQ YTSTSSSQVN
110 120 130 140 150
LGMYHSDTNT RSSRSIASTL KDDSPSVCSE DEISNSSSQK SNAQDETPIA
160 170 180 190 200
YKKSAHSKDS LLPSRSSSLS PPQSRCSTGT TLEKSLNTSG ISNSSGTNNN
210 220 230 240 250
NSNNNNDNEQ KQRNVIHLNS ENYDTTVFKT GWVNKSHGQT VATNYNSSMT
260 270 280 290 300
APSSSSSSSS QNLRNDAYSR NRESRFYGND GSSLKNDDSS STTATNSGND
310 320 330 340 350
VASARSSMAI DPQMLVPDYR LYRAQLKGCV LNLYKSGLNS NIKFFDPTLP
360 370 380 390 400
ASNSSIANEN HQQKKQQTNN QAQAEALHQK QSFGQMGEPI TLDLKYLSEV
410 420 430 440 450
YPHPDLRQDS DGKIISGTIE SLCHTVLFYP GPKQSDVPNE KSLSKTHRAV
460 470 480 490 500
INLLLMFPLL DHFIKFLKVF NQFGLSFTKN KSRLTNNSTQ FYNISPAVDD
510 520 530 540 550
SMTQRLALTA KTILDVFPGF LLDEPMLKTI ISLLDTISLH NDEISNNLKI
560 570 580 590 600
KIANKHNELM KLTAFTRSLP MATSSTHELE IILDPSHFLS LDITTLADEV
610 620 630 640 650
HHINLKFDKV WAPKFDYSLL YDSKFINRRI VSLNPLVFNN DQNIHFLGRL
660 670 680 690 700
LISHLFPTNP EFSKKVTPKV RAELLDKWVQ IGCRFEHLGD MVSWLAVATI
710 720 730 740 750
ICSIPVLRSS SWKYVPDQSL KTIFKDWVPT IIQLERRQRT SKSTSSVFIL
760 770 780 790 800
APPNLDDDFT RANVISYFGD LLIHADDLPS DTKFKYLEKK INRTKNAFHK
810 820 830 840 850
WQQRLQAIDS TRHKTNSTEN VRDNDSPNNV VYQLWKFHLS QPPLNIEGIM
860 870 880 890 900
KLSVQHEPPI IDQKAYSTIG SQRSALVTGS YLPILFNELF PNYSLFPKNT
910 920 930 940 950
LVGAASDAKL PPPRSSARLS KSLSISEPIP IASNSHTMGS LTDDAMSSKN
960 970 980 990 1000
DNNKVTGVGK IDGPVIKEMS SKQSNKQRLL KSVRDVFNID MDVFHISDEL
1010 1020 1030 1040 1050
VFKSVYDNDG KSRPASMVIE TPKRFSQHSS MLINNPATPN QKMRDSLDTT
1060 1070 1080 1090 1100
GRLSKTLENM DFFNNIGQVS DSLKESIIRV VLKSSSLEKI FDLLVLTSNI
1110 1120 1130 1140 1150
FSKLVDTKDL ENYYYHQRQR GHSTRGLSDD NIGLLDYAFV KLTMDNDIFT
1160 1170 1180 1190 1200
ETFFNTYKSF TTTTTVLENM AKRYVGAKSC SVSISKILDR SDDSKMKINE
1210 1220 1230 1240 1250
DTNLVSSSLY DQNFPVWDMK VTDDENINLI YMAKIQIGAA EAILHLVKNH
1260 1270 1280 1290 1300
YSDFTDDLCN NSTLLDIIKI MEQEVSTEWP TRIANSKLQK SLPENFVIET
1310 1320 1330 1340 1350
ENLLTTLTDL FHGIKSAYQK QLYRPIGVNR TQKRITDILN SFNTFSFTDL
1360 1370 1380 1390 1400
NNIIDDPSFS DDMIRSFQKL HSTNYEDILE WIYQLDNFIS KKFNLVSKKD
1410 1420 1430 1440 1450
WIVLFQELEL LSKESLVSFF NYPLHFKSSK LINPGYLQLH EFEISNLFTW
1460 1470 1480 1490 1500
ISTLILKDDN GTESLFFEKL PQSIKLLIKL HTSLTTFFVM EISNVNKSSS
1510 1520 1530 1540 1550
ERLTTCKVIL QILNYIRWKN GSLDLFDSEE DESPHAICPH IPAFIETAIA
1560 1570 1580 1590 1600
HAIISPESRN YELSWIKASE KLSDPTKGTQ NLRSISNVLE KIDDIHIKRF
1610 1620 1630 1640 1650
IEIDDVFSKN CKNLCPCPGW FISRLLEISQ FVPNMSITNS KLINFDKRRF
1660 1670 1680 1690 1700
VNNIISNVLD LIPNEREFPL DIEMSDENPS KRTTFGRILF NNFEDVNKVY
1710 1720 1730 1740 1750
RKKTKKVSES EAISERFQEQ GVFNEILVNE IEKIKREARK LEVLLDQEKI
1760 1770 1780 1790 1800
LKNSAALHQA VPKKNRKSVI ISGTHSDNDH SYNINKNTGQ TPSLGSVMES
1810 1820 1830 1840 1850
NNSARNRRDS RASFSTNRSS VVSNSSHNGV SKKIGGFFRR PFSIGGFNTS
1860 1870 1880 1890 1900
SSNYSLNSIL SQEVSSNKSI LPSILPEVDS MQLHDLKPSY SLKTFEIKSI
1910 1920 1930 1940 1950
MEIINHRNIP AYYYAFKIVM QNGHEYLIQT ASSSDLTEWI KMIKASKRFS
1960 1970 1980 1990 2000
FHSKKYKGKT HNKIFGVPLE DVCERENTLI PTIVVKLLEE IELRGLDEVG
2010 2020 2030 2040 2050
LYRIPGSIGS INALKNAFDE EGATDNSFTL EDDRWFEVNA IAGCFKMYLR
2060 2070 2080 2090 2100
ELPDSLFSHA MVNDFTDLAI KYKAHAMVNE EYKRMMNELL QKLPTCYYQT
2110 2120 2130 2140 2150
LKRIVFHLNK VHQHVVNNKM DASNLAIVFS MSFINQEDLA NSMGSRLGAV
2160
QTILQDFIKN PNDYFKQ
Length:2,167
Mass (Da):245,430
Last modified:February 1, 1995 - v1
Checksum:iDB7F4CD417E898F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35159 Genomic DNA Translation: CAA84524.1
L33832 Genomic DNA Translation: AAA57132.1
U18917 Genomic DNA Translation: AAB64682.1
BK006939 Genomic DNA Translation: DAA07816.1
PIRiS50658
RefSeqiNP_011082.3, NM_001179045.3

Genome annotation databases

EnsemblFungiiYER155C; YER155C; YER155C
GeneIDi856899
KEGGisce:YER155C

Similar proteinsi

Entry informationi

Entry nameiBEM2_YEAST
AccessioniPrimary (citable) accession number: P39960
Secondary accession number(s): D3DM62
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 175 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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