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P39960

- BEM2_YEAST

UniProt

P39960 - BEM2_YEAST

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Protein

GTPase-activating protein BEM2/IPL2

Gene

BEM2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

GTPase-activating protein (GAP) for RHO1 and RHO2. Involved in the control of cellular morphogenesis. Required for proper bud site selection and bud emergence.

GO - Molecular functioni

  1. guanyl-nucleotide exchange factor activity Source: InterPro
  2. Rho GTPase activator activity Source: SGD

GO - Biological processi

  1. actin cytoskeleton organization Source: SGD
  2. establishment of cell polarity Source: SGD
  3. negative regulation of Rho protein signal transduction Source: SGD
  4. positive regulation of Rho GTPase activity Source: GOC
  5. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

BioCyciYEAST:G3O-30316-MONOMER.
ReactomeiREACT_240239. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase-activating protein BEM2/IPL2
Alternative name(s):
Bud emergence protein 2
Gene namesi
Name:BEM2
Synonyms:IPL2, SUP9
Ordered Locus Names:YER155C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER155c.
SGDiS000000957. BEM2.

Subcellular locationi

GO - Cellular componenti

  1. cell cortex Source: SGD
  2. cellular bud tip Source: SGD
  3. cytoplasm Source: SGD
  4. incipient cellular bud site Source: SGD
  5. mating projection tip Source: SGD
  6. plasma membrane Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 21672167GTPase-activating protein BEM2/IPL2PRO_0000068858Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei129 – 1291Phosphoserine2 Publications
Modified residuei283 – 2831Phosphoserine2 Publications
Modified residuei1012 – 10121Phosphoserine2 Publications
Modified residuei1016 – 10161Phosphoserine1 Publication
Modified residuei1038 – 10381Phosphothreonine1 Publication
Modified residuei1046 – 10461Phosphoserine2 Publications
Modified residuei1054 – 10541Phosphoserine1 Publication
Modified residuei1128 – 11281Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39960.
PaxDbiP39960.
PeptideAtlasiP39960.

Expressioni

Gene expression databases

GenevestigatoriP39960.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
BUD14P276373EBI-3517,EBI-20747

Protein-protein interaction databases

BioGridi36905. 295 interactions.
DIPiDIP-6580N.
IntActiP39960. 45 interactions.
MINTiMINT-651655.
STRINGi4932.YER155C.

Structurei

3D structure databases

ProteinModelPortaliP39960.
SMRiP39960. Positions 1948-2144.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini592 – 859268Ras-GEFPROSITE-ProRule annotationAdd
BLAST
Domaini1846 – 1948103PHPROSITE-ProRule annotationAdd
BLAST
Domaini1967 – 2165199Rho-GAPPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi16 – 2510Poly-Ser
Compositional biasi35 – 439Poly-Ser
Compositional biasi58 – 636Poly-His
Compositional biasi198 – 20811Poly-AsnAdd
BLAST
Compositional biasi253 – 2608Poly-Ser
Compositional biasi1161 – 11655Poly-Thr

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 Ras-GEF domain.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG279563.
GeneTreeiENSGT00760000119098.
HOGENOMiHOG000095195.
InParanoidiP39960.
OMAiFGWIIEN.
OrthoDBiEOG769ZST.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
InterProiIPR001849. PH_domain.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF48366. SSF48366. 5 hits.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39960-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKGLLWSKNR KSSTASASSS STSTSHKTTT ASTASSSSPS SSSQTIRNST
60 70 80 90 100
SGASPYMHSH HHHGQGHSHH RGEDNNRDKR KSSVFPPSKQ YTSTSSSQVN
110 120 130 140 150
LGMYHSDTNT RSSRSIASTL KDDSPSVCSE DEISNSSSQK SNAQDETPIA
160 170 180 190 200
YKKSAHSKDS LLPSRSSSLS PPQSRCSTGT TLEKSLNTSG ISNSSGTNNN
210 220 230 240 250
NSNNNNDNEQ KQRNVIHLNS ENYDTTVFKT GWVNKSHGQT VATNYNSSMT
260 270 280 290 300
APSSSSSSSS QNLRNDAYSR NRESRFYGND GSSLKNDDSS STTATNSGND
310 320 330 340 350
VASARSSMAI DPQMLVPDYR LYRAQLKGCV LNLYKSGLNS NIKFFDPTLP
360 370 380 390 400
ASNSSIANEN HQQKKQQTNN QAQAEALHQK QSFGQMGEPI TLDLKYLSEV
410 420 430 440 450
YPHPDLRQDS DGKIISGTIE SLCHTVLFYP GPKQSDVPNE KSLSKTHRAV
460 470 480 490 500
INLLLMFPLL DHFIKFLKVF NQFGLSFTKN KSRLTNNSTQ FYNISPAVDD
510 520 530 540 550
SMTQRLALTA KTILDVFPGF LLDEPMLKTI ISLLDTISLH NDEISNNLKI
560 570 580 590 600
KIANKHNELM KLTAFTRSLP MATSSTHELE IILDPSHFLS LDITTLADEV
610 620 630 640 650
HHINLKFDKV WAPKFDYSLL YDSKFINRRI VSLNPLVFNN DQNIHFLGRL
660 670 680 690 700
LISHLFPTNP EFSKKVTPKV RAELLDKWVQ IGCRFEHLGD MVSWLAVATI
710 720 730 740 750
ICSIPVLRSS SWKYVPDQSL KTIFKDWVPT IIQLERRQRT SKSTSSVFIL
760 770 780 790 800
APPNLDDDFT RANVISYFGD LLIHADDLPS DTKFKYLEKK INRTKNAFHK
810 820 830 840 850
WQQRLQAIDS TRHKTNSTEN VRDNDSPNNV VYQLWKFHLS QPPLNIEGIM
860 870 880 890 900
KLSVQHEPPI IDQKAYSTIG SQRSALVTGS YLPILFNELF PNYSLFPKNT
910 920 930 940 950
LVGAASDAKL PPPRSSARLS KSLSISEPIP IASNSHTMGS LTDDAMSSKN
960 970 980 990 1000
DNNKVTGVGK IDGPVIKEMS SKQSNKQRLL KSVRDVFNID MDVFHISDEL
1010 1020 1030 1040 1050
VFKSVYDNDG KSRPASMVIE TPKRFSQHSS MLINNPATPN QKMRDSLDTT
1060 1070 1080 1090 1100
GRLSKTLENM DFFNNIGQVS DSLKESIIRV VLKSSSLEKI FDLLVLTSNI
1110 1120 1130 1140 1150
FSKLVDTKDL ENYYYHQRQR GHSTRGLSDD NIGLLDYAFV KLTMDNDIFT
1160 1170 1180 1190 1200
ETFFNTYKSF TTTTTVLENM AKRYVGAKSC SVSISKILDR SDDSKMKINE
1210 1220 1230 1240 1250
DTNLVSSSLY DQNFPVWDMK VTDDENINLI YMAKIQIGAA EAILHLVKNH
1260 1270 1280 1290 1300
YSDFTDDLCN NSTLLDIIKI MEQEVSTEWP TRIANSKLQK SLPENFVIET
1310 1320 1330 1340 1350
ENLLTTLTDL FHGIKSAYQK QLYRPIGVNR TQKRITDILN SFNTFSFTDL
1360 1370 1380 1390 1400
NNIIDDPSFS DDMIRSFQKL HSTNYEDILE WIYQLDNFIS KKFNLVSKKD
1410 1420 1430 1440 1450
WIVLFQELEL LSKESLVSFF NYPLHFKSSK LINPGYLQLH EFEISNLFTW
1460 1470 1480 1490 1500
ISTLILKDDN GTESLFFEKL PQSIKLLIKL HTSLTTFFVM EISNVNKSSS
1510 1520 1530 1540 1550
ERLTTCKVIL QILNYIRWKN GSLDLFDSEE DESPHAICPH IPAFIETAIA
1560 1570 1580 1590 1600
HAIISPESRN YELSWIKASE KLSDPTKGTQ NLRSISNVLE KIDDIHIKRF
1610 1620 1630 1640 1650
IEIDDVFSKN CKNLCPCPGW FISRLLEISQ FVPNMSITNS KLINFDKRRF
1660 1670 1680 1690 1700
VNNIISNVLD LIPNEREFPL DIEMSDENPS KRTTFGRILF NNFEDVNKVY
1710 1720 1730 1740 1750
RKKTKKVSES EAISERFQEQ GVFNEILVNE IEKIKREARK LEVLLDQEKI
1760 1770 1780 1790 1800
LKNSAALHQA VPKKNRKSVI ISGTHSDNDH SYNINKNTGQ TPSLGSVMES
1810 1820 1830 1840 1850
NNSARNRRDS RASFSTNRSS VVSNSSHNGV SKKIGGFFRR PFSIGGFNTS
1860 1870 1880 1890 1900
SSNYSLNSIL SQEVSSNKSI LPSILPEVDS MQLHDLKPSY SLKTFEIKSI
1910 1920 1930 1940 1950
MEIINHRNIP AYYYAFKIVM QNGHEYLIQT ASSSDLTEWI KMIKASKRFS
1960 1970 1980 1990 2000
FHSKKYKGKT HNKIFGVPLE DVCERENTLI PTIVVKLLEE IELRGLDEVG
2010 2020 2030 2040 2050
LYRIPGSIGS INALKNAFDE EGATDNSFTL EDDRWFEVNA IAGCFKMYLR
2060 2070 2080 2090 2100
ELPDSLFSHA MVNDFTDLAI KYKAHAMVNE EYKRMMNELL QKLPTCYYQT
2110 2120 2130 2140 2150
LKRIVFHLNK VHQHVVNNKM DASNLAIVFS MSFINQEDLA NSMGSRLGAV
2160
QTILQDFIKN PNDYFKQ
Length:2,167
Mass (Da):245,430
Last modified:February 1, 1995 - v1
Checksum:iDB7F4CD417E898F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35159 Genomic DNA. Translation: CAA84524.1.
L33832 Genomic DNA. Translation: AAA57132.1.
U18917 Genomic DNA. Translation: AAB64682.1.
BK006939 Genomic DNA. Translation: DAA07816.1.
PIRiS50658.
RefSeqiNP_011082.3. NM_001179045.3.

Genome annotation databases

EnsemblFungiiYER155C; YER155C; YER155C.
GeneIDi856899.
KEGGisce:YER155C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35159 Genomic DNA. Translation: CAA84524.1 .
L33832 Genomic DNA. Translation: AAA57132.1 .
U18917 Genomic DNA. Translation: AAB64682.1 .
BK006939 Genomic DNA. Translation: DAA07816.1 .
PIRi S50658.
RefSeqi NP_011082.3. NM_001179045.3.

3D structure databases

ProteinModelPortali P39960.
SMRi P39960. Positions 1948-2144.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36905. 295 interactions.
DIPi DIP-6580N.
IntActi P39960. 45 interactions.
MINTi MINT-651655.
STRINGi 4932.YER155C.

Proteomic databases

MaxQBi P39960.
PaxDbi P39960.
PeptideAtlasi P39960.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER155C ; YER155C ; YER155C .
GeneIDi 856899.
KEGGi sce:YER155C.

Organism-specific databases

CYGDi YER155c.
SGDi S000000957. BEM2.

Phylogenomic databases

eggNOGi NOG279563.
GeneTreei ENSGT00760000119098.
HOGENOMi HOG000095195.
InParanoidi P39960.
OMAi FGWIIEN.
OrthoDBi EOG769ZST.

Enzyme and pathway databases

BioCyci YEAST:G3O-30316-MONOMER.
Reactomei REACT_240239. Rho GTPase cycle.

Miscellaneous databases

NextBioi 983319.

Gene expression databases

Genevestigatori P39960.

Family and domain databases

Gene3Di 1.10.555.10. 1 hit.
InterProi IPR001849. PH_domain.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view ]
Pfami PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view ]
SMARTi SM00233. PH. 1 hit.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view ]
SUPFAMi SSF48350. SSF48350. 1 hit.
SSF48366. SSF48366. 5 hits.
PROSITEi PS50003. PH_DOMAIN. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Control of cellular morphogenesis by the Ip12/Bem2 GTPase-activating protein: possible role of protein phosphorylation."
    Kim Y., Francisco L., Chen G., Marcotte E., Chan C.S.
    J. Cell Biol. 127:1381-1394(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Interactions between the bud emergence proteins Bem1p and Bem2p and Rho-type GTPases in yeast."
    Peterson J., Zheng Y., Bender L., Myers A., Cerione R., Bender A.
    J. Cell Biol. 127:1395-1406(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Isolation and characterization of chromosome-gain and increase-in-ploidy mutants in yeast."
    Chan C.S., Botstein D.
    Genetics 135:677-691(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1012; SER-1016 AND SER-1046, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1012; SER-1046 AND SER-1054, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-129 AND SER-283, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-129; SER-283; THR-1038 AND SER-1128, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBEM2_YEAST
AccessioniPrimary (citable) accession number: P39960
Secondary accession number(s): D3DM62
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 26, 2014
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1230 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3