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P39960 (BEM2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GTPase-activating protein BEM2/IPL2
Alternative name(s):
Bud emergence protein 2
Gene names
Name:BEM2
Synonyms:IPL2, SUP9
Ordered Locus Names:YER155C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length2167 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

GTPase-activating protein (GAP) for RHO1 and RHO2. Involved in the control of cellular morphogenesis. Required for proper bud site selection and bud emergence.

Miscellaneous

Present with 1230 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 PH domain.

Contains 1 Ras-GEF domain.

Contains 1 Rho-GAP domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

BUD14P276373EBI-3517,EBI-20747

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 21672167GTPase-activating protein BEM2/IPL2
PRO_0000068858

Regions

Domain592 – 859268Ras-GEF
Domain1846 – 1948103PH
Domain1967 – 2165199Rho-GAP
Compositional bias16 – 2510Poly-Ser
Compositional bias35 – 439Poly-Ser
Compositional bias58 – 636Poly-His
Compositional bias198 – 20811Poly-Asn
Compositional bias253 – 2608Poly-Ser
Compositional bias1161 – 11655Poly-Thr

Amino acid modifications

Modified residue1291Phosphoserine Ref.9 Ref.10
Modified residue2831Phosphoserine Ref.9 Ref.10
Modified residue10121Phosphoserine Ref.7 Ref.8
Modified residue10161Phosphoserine Ref.7
Modified residue10381Phosphothreonine Ref.10
Modified residue10461Phosphoserine Ref.7 Ref.8
Modified residue10541Phosphoserine Ref.8
Modified residue11281Phosphoserine Ref.10

Sequences

Sequence LengthMass (Da)Tools
P39960 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: DB7F4CD417E898F0

FASTA2,167245,430
        10         20         30         40         50         60 
MKGLLWSKNR KSSTASASSS STSTSHKTTT ASTASSSSPS SSSQTIRNST SGASPYMHSH 

        70         80         90        100        110        120 
HHHGQGHSHH RGEDNNRDKR KSSVFPPSKQ YTSTSSSQVN LGMYHSDTNT RSSRSIASTL 

       130        140        150        160        170        180 
KDDSPSVCSE DEISNSSSQK SNAQDETPIA YKKSAHSKDS LLPSRSSSLS PPQSRCSTGT 

       190        200        210        220        230        240 
TLEKSLNTSG ISNSSGTNNN NSNNNNDNEQ KQRNVIHLNS ENYDTTVFKT GWVNKSHGQT 

       250        260        270        280        290        300 
VATNYNSSMT APSSSSSSSS QNLRNDAYSR NRESRFYGND GSSLKNDDSS STTATNSGND 

       310        320        330        340        350        360 
VASARSSMAI DPQMLVPDYR LYRAQLKGCV LNLYKSGLNS NIKFFDPTLP ASNSSIANEN 

       370        380        390        400        410        420 
HQQKKQQTNN QAQAEALHQK QSFGQMGEPI TLDLKYLSEV YPHPDLRQDS DGKIISGTIE 

       430        440        450        460        470        480 
SLCHTVLFYP GPKQSDVPNE KSLSKTHRAV INLLLMFPLL DHFIKFLKVF NQFGLSFTKN 

       490        500        510        520        530        540 
KSRLTNNSTQ FYNISPAVDD SMTQRLALTA KTILDVFPGF LLDEPMLKTI ISLLDTISLH 

       550        560        570        580        590        600 
NDEISNNLKI KIANKHNELM KLTAFTRSLP MATSSTHELE IILDPSHFLS LDITTLADEV 

       610        620        630        640        650        660 
HHINLKFDKV WAPKFDYSLL YDSKFINRRI VSLNPLVFNN DQNIHFLGRL LISHLFPTNP 

       670        680        690        700        710        720 
EFSKKVTPKV RAELLDKWVQ IGCRFEHLGD MVSWLAVATI ICSIPVLRSS SWKYVPDQSL 

       730        740        750        760        770        780 
KTIFKDWVPT IIQLERRQRT SKSTSSVFIL APPNLDDDFT RANVISYFGD LLIHADDLPS 

       790        800        810        820        830        840 
DTKFKYLEKK INRTKNAFHK WQQRLQAIDS TRHKTNSTEN VRDNDSPNNV VYQLWKFHLS 

       850        860        870        880        890        900 
QPPLNIEGIM KLSVQHEPPI IDQKAYSTIG SQRSALVTGS YLPILFNELF PNYSLFPKNT 

       910        920        930        940        950        960 
LVGAASDAKL PPPRSSARLS KSLSISEPIP IASNSHTMGS LTDDAMSSKN DNNKVTGVGK 

       970        980        990       1000       1010       1020 
IDGPVIKEMS SKQSNKQRLL KSVRDVFNID MDVFHISDEL VFKSVYDNDG KSRPASMVIE 

      1030       1040       1050       1060       1070       1080 
TPKRFSQHSS MLINNPATPN QKMRDSLDTT GRLSKTLENM DFFNNIGQVS DSLKESIIRV 

      1090       1100       1110       1120       1130       1140 
VLKSSSLEKI FDLLVLTSNI FSKLVDTKDL ENYYYHQRQR GHSTRGLSDD NIGLLDYAFV 

      1150       1160       1170       1180       1190       1200 
KLTMDNDIFT ETFFNTYKSF TTTTTVLENM AKRYVGAKSC SVSISKILDR SDDSKMKINE 

      1210       1220       1230       1240       1250       1260 
DTNLVSSSLY DQNFPVWDMK VTDDENINLI YMAKIQIGAA EAILHLVKNH YSDFTDDLCN 

      1270       1280       1290       1300       1310       1320 
NSTLLDIIKI MEQEVSTEWP TRIANSKLQK SLPENFVIET ENLLTTLTDL FHGIKSAYQK 

      1330       1340       1350       1360       1370       1380 
QLYRPIGVNR TQKRITDILN SFNTFSFTDL NNIIDDPSFS DDMIRSFQKL HSTNYEDILE 

      1390       1400       1410       1420       1430       1440 
WIYQLDNFIS KKFNLVSKKD WIVLFQELEL LSKESLVSFF NYPLHFKSSK LINPGYLQLH 

      1450       1460       1470       1480       1490       1500 
EFEISNLFTW ISTLILKDDN GTESLFFEKL PQSIKLLIKL HTSLTTFFVM EISNVNKSSS 

      1510       1520       1530       1540       1550       1560 
ERLTTCKVIL QILNYIRWKN GSLDLFDSEE DESPHAICPH IPAFIETAIA HAIISPESRN 

      1570       1580       1590       1600       1610       1620 
YELSWIKASE KLSDPTKGTQ NLRSISNVLE KIDDIHIKRF IEIDDVFSKN CKNLCPCPGW 

      1630       1640       1650       1660       1670       1680 
FISRLLEISQ FVPNMSITNS KLINFDKRRF VNNIISNVLD LIPNEREFPL DIEMSDENPS 

      1690       1700       1710       1720       1730       1740 
KRTTFGRILF NNFEDVNKVY RKKTKKVSES EAISERFQEQ GVFNEILVNE IEKIKREARK 

      1750       1760       1770       1780       1790       1800 
LEVLLDQEKI LKNSAALHQA VPKKNRKSVI ISGTHSDNDH SYNINKNTGQ TPSLGSVMES 

      1810       1820       1830       1840       1850       1860 
NNSARNRRDS RASFSTNRSS VVSNSSHNGV SKKIGGFFRR PFSIGGFNTS SSNYSLNSIL 

      1870       1880       1890       1900       1910       1920 
SQEVSSNKSI LPSILPEVDS MQLHDLKPSY SLKTFEIKSI MEIINHRNIP AYYYAFKIVM 

      1930       1940       1950       1960       1970       1980 
QNGHEYLIQT ASSSDLTEWI KMIKASKRFS FHSKKYKGKT HNKIFGVPLE DVCERENTLI 

      1990       2000       2010       2020       2030       2040 
PTIVVKLLEE IELRGLDEVG LYRIPGSIGS INALKNAFDE EGATDNSFTL EDDRWFEVNA 

      2050       2060       2070       2080       2090       2100 
IAGCFKMYLR ELPDSLFSHA MVNDFTDLAI KYKAHAMVNE EYKRMMNELL QKLPTCYYQT 

      2110       2120       2130       2140       2150       2160 
LKRIVFHLNK VHQHVVNNKM DASNLAIVFS MSFINQEDLA NSMGSRLGAV QTILQDFIKN 


PNDYFKQ 

« Hide

References

« Hide 'large scale' references
[1]"Control of cellular morphogenesis by the Ip12/Bem2 GTPase-activating protein: possible role of protein phosphorylation."
Kim Y., Francisco L., Chen G., Marcotte E., Chan C.S.
J. Cell Biol. 127:1381-1394(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Interactions between the bud emergence proteins Bem1p and Bem2p and Rho-type GTPases in yeast."
Peterson J., Zheng Y., Bender L., Myers A., Cerione R., Bender A.
J. Cell Biol. 127:1395-1406(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Isolation and characterization of chromosome-gain and increase-in-ploidy mutants in yeast."
Chan C.S., Botstein D.
Genetics 135:677-691(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1012; SER-1016 AND SER-1046, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[8]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1012; SER-1046 AND SER-1054, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-129 AND SER-283, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-129; SER-283; THR-1038 AND SER-1128, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z35159 Genomic DNA. Translation: CAA84524.1.
L33832 Genomic DNA. Translation: AAA57132.1.
U18917 Genomic DNA. Translation: AAB64682.1.
BK006939 Genomic DNA. Translation: DAA07816.1.
PIRS50658.
RefSeqNP_011082.3. NM_001179045.3.

3D structure databases

ProteinModelPortalP39960.
SMRP39960. Positions 1886-2144.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36905. 294 interactions.
DIPDIP-6580N.
IntActP39960. 45 interactions.
MINTMINT-651655.
STRING4932.YER155C.

Proteomic databases

PaxDbP39960.
PeptideAtlasP39960.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYER155C; YER155C; YER155C.
GeneID856899.
KEGGsce:YER155C.

Organism-specific databases

CYGDYER155c.
SGDS000000957. BEM2.

Phylogenomic databases

eggNOGNOG279563.
GeneTreeENSGT00730000110473.
HOGENOMHOG000095195.
OMAFGWIIEN.
OrthoDBEOG769ZST.

Enzyme and pathway databases

BioCycYEAST:G3O-30316-MONOMER.

Gene expression databases

GenevestigatorP39960.

Family and domain databases

Gene3D1.10.555.10. 1 hit.
InterProIPR001849. Pleckstrin_homology.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR023578. Ras_GEF_dom.
IPR001895. RasGRF_CDC25.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamPF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTSM00233. PH. 1 hit.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMSSF48350. SSF48350. 1 hit.
SSF48366. SSF48366. 5 hits.
PROSITEPS50003. PH_DOMAIN. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio983319.

Entry information

Entry nameBEM2_YEAST
AccessionPrimary (citable) accession number: P39960
Secondary accession number(s): D3DM62
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families