GTPase-activating protein BEM2/IPL2 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P39960 (BEM2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 125. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
GTPase-activating protein BEM2/IPL2

Alternative name(s):
Bud emergence protein 2

Gene names

Name:	BEM2
Synonyms:	IPL2, SUP9
Ordered Locus Names:	YER155C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	2167 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

GTPase-activating protein (GAP) for RHO1 and RHO2. Involved in the control of cellular morphogenesis. Required for proper bud site selection and bud emergence.

Miscellaneous

Present with 1230 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 PH domain.

Contains 1 Ras-GEF domain.

Contains 1 Rho-GAP domain.

Ontologies

Keywords
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin cytoskeleton organization Inferred from genetic interaction PubMed 7579704. Source: SGD establishment of cell polarity Traceable author statement PubMed 9891811. Source: SGD fungal-type cell wall organization Traceable author statement PubMed 9891811. Source: SGD negative regulation of Rho protein signal transduction Inferred from mutant phenotype PubMed 17914457. Source: SGD small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cell cortex Inferred from direct assay PubMed 17914457. Source: SGD cellular bud tip Inferred from direct assay PubMed 17914457. Source: SGD incipient cellular bud site Inferred from direct assay PubMed 17914457. Source: SGD mating projection tip Inferred from direct assay PubMed 17914457. Source: SGD mitochondrion Inferred from direct assay PubMed 14576278 PubMed 16823961. Source: SGD plasma membrane Inferred from direct assay PubMed 17914457. Source: SGD
Molecular_function	Rho GTPase activator activity Inferred from direct assay Ref.2. Source: SGD guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BUD14	P27637	3	EBI-3517,EBI-20747

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 2167

2167

GTPase-activating protein BEM2/IPL2

PRO_0000068858

Regions

Domain

592 – 859

268

Ras-GEF

Domain

1846 – 1948

103

Domain

1967 – 2165

199

Rho-GAP

Compositional bias

16 – 25

Poly-Ser

Compositional bias

35 – 43

Poly-Ser

Compositional bias

58 – 63

Poly-His

Compositional bias

198 – 208

Poly-Asn

Compositional bias

253 – 260

Poly-Ser

Compositional bias

1161 – 1165

Poly-Thr

Amino acid modifications

Modified residue

Phosphoserine Ref.10

Modified residue

126

Phosphoserine Ref.10

Modified residue

129

Phosphoserine Ref.10

Modified residue

154

Phosphoserine Ref.10

Modified residue

157

Phosphoserine Ref.10

Modified residue

177

Phosphoserine Ref.10

Modified residue

283

Phosphoserine Ref.9 Ref.10

Modified residue

746

Phosphoserine Ref.10

Modified residue

920

Phosphoserine Ref.8

Modified residue

1012

Phosphoserine Ref.7 Ref.8 Ref.10

Modified residue

1016

Phosphoserine Ref.7 Ref.10

Modified residue

1046

Phosphoserine Ref.7 Ref.8 Ref.9 Ref.10

Modified residue

1054

Phosphoserine Ref.8

Sequences

Sequence

Length

Mass (Da)

Tools

P39960 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: DB7F4CD417E898F0
Show »

FASTA

2,167

245,430

        10         20         30         40         50         60 
MKGLLWSKNR KSSTASASSS STSTSHKTTT ASTASSSSPS SSSQTIRNST SGASPYMHSH 

        70         80         90        100        110        120 
HHHGQGHSHH RGEDNNRDKR KSSVFPPSKQ YTSTSSSQVN LGMYHSDTNT RSSRSIASTL 

       130        140        150        160        170        180 
KDDSPSVCSE DEISNSSSQK SNAQDETPIA YKKSAHSKDS LLPSRSSSLS PPQSRCSTGT 

       190        200        210        220        230        240 
TLEKSLNTSG ISNSSGTNNN NSNNNNDNEQ KQRNVIHLNS ENYDTTVFKT GWVNKSHGQT 

       250        260        270        280        290        300 
VATNYNSSMT APSSSSSSSS QNLRNDAYSR NRESRFYGND GSSLKNDDSS STTATNSGND 

       310        320        330        340        350        360 
VASARSSMAI DPQMLVPDYR LYRAQLKGCV LNLYKSGLNS NIKFFDPTLP ASNSSIANEN 

       370        380        390        400        410        420 
HQQKKQQTNN QAQAEALHQK QSFGQMGEPI TLDLKYLSEV YPHPDLRQDS DGKIISGTIE 

       430        440        450        460        470        480 
SLCHTVLFYP GPKQSDVPNE KSLSKTHRAV INLLLMFPLL DHFIKFLKVF NQFGLSFTKN 

       490        500        510        520        530        540 
KSRLTNNSTQ FYNISPAVDD SMTQRLALTA KTILDVFPGF LLDEPMLKTI ISLLDTISLH 

       550        560        570        580        590        600 
NDEISNNLKI KIANKHNELM KLTAFTRSLP MATSSTHELE IILDPSHFLS LDITTLADEV 

       610        620        630        640        650        660 
HHINLKFDKV WAPKFDYSLL YDSKFINRRI VSLNPLVFNN DQNIHFLGRL LISHLFPTNP 

       670        680        690        700        710        720 
EFSKKVTPKV RAELLDKWVQ IGCRFEHLGD MVSWLAVATI ICSIPVLRSS SWKYVPDQSL 

       730        740        750        760        770        780 
KTIFKDWVPT IIQLERRQRT SKSTSSVFIL APPNLDDDFT RANVISYFGD LLIHADDLPS 

       790        800        810        820        830        840 
DTKFKYLEKK INRTKNAFHK WQQRLQAIDS TRHKTNSTEN VRDNDSPNNV VYQLWKFHLS 

       850        860        870        880        890        900 
QPPLNIEGIM KLSVQHEPPI IDQKAYSTIG SQRSALVTGS YLPILFNELF PNYSLFPKNT 

       910        920        930        940        950        960 
LVGAASDAKL PPPRSSARLS KSLSISEPIP IASNSHTMGS LTDDAMSSKN DNNKVTGVGK 

       970        980        990       1000       1010       1020 
IDGPVIKEMS SKQSNKQRLL KSVRDVFNID MDVFHISDEL VFKSVYDNDG KSRPASMVIE 

      1030       1040       1050       1060       1070       1080 
TPKRFSQHSS MLINNPATPN QKMRDSLDTT GRLSKTLENM DFFNNIGQVS DSLKESIIRV 

      1090       1100       1110       1120       1130       1140 
VLKSSSLEKI FDLLVLTSNI FSKLVDTKDL ENYYYHQRQR GHSTRGLSDD NIGLLDYAFV 

      1150       1160       1170       1180       1190       1200 
KLTMDNDIFT ETFFNTYKSF TTTTTVLENM AKRYVGAKSC SVSISKILDR SDDSKMKINE 

      1210       1220       1230       1240       1250       1260 
DTNLVSSSLY DQNFPVWDMK VTDDENINLI YMAKIQIGAA EAILHLVKNH YSDFTDDLCN 

      1270       1280       1290       1300       1310       1320 
NSTLLDIIKI MEQEVSTEWP TRIANSKLQK SLPENFVIET ENLLTTLTDL FHGIKSAYQK 

      1330       1340       1350       1360       1370       1380 
QLYRPIGVNR TQKRITDILN SFNTFSFTDL NNIIDDPSFS DDMIRSFQKL HSTNYEDILE 

      1390       1400       1410       1420       1430       1440 
WIYQLDNFIS KKFNLVSKKD WIVLFQELEL LSKESLVSFF NYPLHFKSSK LINPGYLQLH 

      1450       1460       1470       1480       1490       1500 
EFEISNLFTW ISTLILKDDN GTESLFFEKL PQSIKLLIKL HTSLTTFFVM EISNVNKSSS 

      1510       1520       1530       1540       1550       1560 
ERLTTCKVIL QILNYIRWKN GSLDLFDSEE DESPHAICPH IPAFIETAIA HAIISPESRN 

      1570       1580       1590       1600       1610       1620 
YELSWIKASE KLSDPTKGTQ NLRSISNVLE KIDDIHIKRF IEIDDVFSKN CKNLCPCPGW 

      1630       1640       1650       1660       1670       1680 
FISRLLEISQ FVPNMSITNS KLINFDKRRF VNNIISNVLD LIPNEREFPL DIEMSDENPS 

      1690       1700       1710       1720       1730       1740 
KRTTFGRILF NNFEDVNKVY RKKTKKVSES EAISERFQEQ GVFNEILVNE IEKIKREARK 

      1750       1760       1770       1780       1790       1800 
LEVLLDQEKI LKNSAALHQA VPKKNRKSVI ISGTHSDNDH SYNINKNTGQ TPSLGSVMES 

      1810       1820       1830       1840       1850       1860 
NNSARNRRDS RASFSTNRSS VVSNSSHNGV SKKIGGFFRR PFSIGGFNTS SSNYSLNSIL 

      1870       1880       1890       1900       1910       1920 
SQEVSSNKSI LPSILPEVDS MQLHDLKPSY SLKTFEIKSI MEIINHRNIP AYYYAFKIVM 

      1930       1940       1950       1960       1970       1980 
QNGHEYLIQT ASSSDLTEWI KMIKASKRFS FHSKKYKGKT HNKIFGVPLE DVCERENTLI 

      1990       2000       2010       2020       2030       2040 
PTIVVKLLEE IELRGLDEVG LYRIPGSIGS INALKNAFDE EGATDNSFTL EDDRWFEVNA 

      2050       2060       2070       2080       2090       2100 
IAGCFKMYLR ELPDSLFSHA MVNDFTDLAI KYKAHAMVNE EYKRMMNELL QKLPTCYYQT 

      2110       2120       2130       2140       2150       2160 
LKRIVFHLNK VHQHVVNNKM DASNLAIVFS MSFINQEDLA NSMGSRLGAV QTILQDFIKN 


PNDYFKQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Control of cellular morphogenesis by the Ip12/Bem2 GTPase-activating protein: possible role of protein phosphorylation." Kim Y., Francisco L., Chen G., Marcotte E., Chan C.S. J. Cell Biol. 127:1381-1394(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Interactions between the bud emergence proteins Bem1p and Bem2p and Rho-type GTPases in yeast." Peterson J., Zheng Y., Bender L., Myers A., Cerione R., Bender A. J. Cell Biol. 127:1395-1406(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Isolation and characterization of chromosome-gain and increase-in-ploidy mutants in yeast." Chan C.S., Botstein D. Genetics 135:677-691(1993) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1012; SER-1016 AND SER-1046, MASS SPECTROMETRY. Strain: ADR376.
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-920; SER-1012; SER-1046 AND SER-1054, MASS SPECTROMETRY.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-283 AND SER-1046, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95; SER-126; SER-129; SER-154; SER-157; SER-177; SER-283; SER-746; SER-1012; SER-1016 AND SER-1046, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z35159 Genomic DNA. Translation: CAA84524.1. L33832 Genomic DNA. Translation: AAA57132.1. U18917 Genomic DNA. Translation: AAB64682.1. BK006939 Genomic DNA. Translation: DAA07816.1.
PIR	S50658.
RefSeq	NP_011082.3. NM_001179045.3.
3D structure databases
ProteinModelPortal	P39960.
SMR	P39960. Positions 1948-2144.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6580N.
IntAct	P39960. 44 interactions.
MINT	MINT-651655.
STRING	4932.YER155C.
Proteomic databases
PaxDb	P39960.
PeptideAtlas	P39960.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER155C; YER155C; YER155C.
GeneID	856899.
KEGG	sce:YER155C.
Organism-specific databases
CYGD	YER155c.
SGD	S000000957. BEM2.
Phylogenomic databases
eggNOG	NOG279563.
GeneTree	ENSGT00700000104152.
HOGENOM	HOG000095195.
OMA	HIMAPRG.
OrthoDB	EOG44F9J6.
Enzyme and pathway databases
BioCyc	YEAST:G3O-30316-MONOMER.
Gene expression databases
Genevestigator	P39960.
GermOnline	YER155C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.555.10. 1 hit.
InterPro	IPR001849. Pleckstrin_homology. IPR000651. Ras-like_Gua-exchang_fac_N. IPR023578. Ras_GEF_dom. IPR001895. RasGRF_CDC25. IPR008936. Rho_GTPase_activation_prot. IPR000198. RhoGAP_dom. [Graphical view]
Pfam	PF00617. RasGEF. 1 hit. PF00618. RasGEF_N. 1 hit. PF00620. RhoGAP. 1 hit. [Graphical view]
SMART	SM00233. PH. 1 hit. SM00147. RasGEF. 1 hit. SM00229. RasGEFN. 1 hit. SM00324. RhoGAP. 1 hit. [Graphical view]
SUPFAM	SSF48366. Ras_GEF. 2 hits. SSF48350. Rho_GAP. 1 hit.
PROSITE	PS50003. PH_DOMAIN. 1 hit. PS00720. RASGEF. False negative. PS50009. RASGEF_CAT. 1 hit. PS50238. RHOGAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	983319.

Entry information

Entry name

BEM2_YEAST

Accession

Primary (citable) accession number: P39960
Secondary accession number(s): D3DM62

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 29, 2013
This is version 125 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents