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P39958

- GDI1_YEAST

UniProt

P39958 - GDI1_YEAST

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Protein

Rab GDP-dissociation inhibitor

Gene
GDI1, SEC19, YER136W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Regulates the GDP/GTP exchange reaction of SEC4 by inhibiting the dissociation of GDP from it, and the subsequent binding of GTP to SEC4. Plays an essential role in the yeast secretory pathway.1 Publication

GO - Molecular functioni

  1. GTPase activator activity Source: UniProtKB-KW
  2. protein binding Source: IntAct
  3. Rab GDP-dissociation inhibitor activity Source: SGD

GO - Biological processi

  1. protein transport Source: UniProtKB-KW
  2. regulation of catalytic activity Source: GOC
  3. vesicle-mediated transport Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30297-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Rab GDP-dissociation inhibitor
Short name:
Rab GDI
Alternative name(s):
Secretory pathway GDP dissociation inhibitor
Gene namesi
Name:GDI1
Synonyms:SEC19
Ordered Locus Names:YER136W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER136w.
SGDiS000000938. GDI1.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 451451Rab GDP-dissociation inhibitorPRO_0000056684Add
BLAST

Proteomic databases

MaxQBiP39958.
PaxDbiP39958.
PeptideAtlasiP39958.

2D gel databases

UCD-2DPAGEP39958.

Expressioni

Gene expression databases

GenevestigatoriP39958.

Interactioni

Subunit structurei

Interacts with the GDP-bound form of Rab GTPase YPT1. Interacts with YPT10.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
YPT1P011235EBI-7517,EBI-29496
YPT10P381464EBI-7517,EBI-29357
YPT31P385556EBI-7517,EBI-29379
YPT52P360183EBI-7517,EBI-29407
YPT6Q992603EBI-7517,EBI-29503

Protein-protein interaction databases

BioGridi36882. 35 interactions.
DIPiDIP-785N.
IntActiP39958. 16 interactions.
MINTiMINT-502785.
STRINGi4932.YER136W.

Structurei

Secondary structure

1
451
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi11 – 155
Helixi19 – 3012
Beta strandi35 – 384
Beta strandi40 – 445
Helixi46 – 483
Helixi53 – 608
Helixi67 – 748
Helixi77 – 793
Beta strandi82 – 854
Beta strandi88 – 903
Helixi94 – 1018
Helixi104 – 1063
Beta strandi110 – 1123
Beta strandi116 – 1205
Beta strandi123 – 1264
Helixi131 – 1366
Beta strandi138 – 1403
Helixi142 – 15716
Helixi163 – 1653
Turni171 – 1733
Helixi176 – 1827
Helixi187 – 19610
Beta strandi201 – 2033
Helixi204 – 2074
Beta strandi208 – 2103
Helixi211 – 22717
Beta strandi231 – 2355
Helixi241 – 25212
Beta strandi256 – 2583
Beta strandi265 – 2684
Turni270 – 2723
Beta strandi275 – 2806
Beta strandi283 – 2864
Beta strandi290 – 2923
Helixi294 – 2963
Helixi298 – 3003
Beta strandi301 – 31717
Beta strandi322 – 3243
Beta strandi326 – 3327
Helixi334 – 3363
Beta strandi343 – 3497
Helixi350 – 3523
Beta strandi360 – 3678
Helixi373 – 3764
Helixi378 – 3814
Helixi382 – 3843
Beta strandi388 – 40215
Turni405 – 4073
Beta strandi408 – 4114
Beta strandi419 – 4213
Helixi422 – 43615

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UKVX-ray1.50G1-451[»]
2BCGX-ray1.48G1-451[»]
3CPHX-ray2.90G/H1-451[»]
3CPIX-ray2.30G/H1-451[»]
3CPJX-ray2.35G1-451[»]
ProteinModelPortaliP39958.
SMRiP39958. Positions 5-446.

Miscellaneous databases

EvolutionaryTraceiP39958.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni106 – 1127Interaction with YPT1
Regioni234 – 25926Interaction with YPT1Add
BLAST

Sequence similaritiesi

Belongs to the Rab GDI family.

Phylogenomic databases

eggNOGiCOG5044.
GeneTreeiENSGT00530000063044.
HOGENOMiHOG000163825.
KOiK17255.
OMAiKKYGNYN.
OrthoDBiEOG72C58R.

Family and domain databases

InterProiIPR018203. GDP_dissociation_inhibitor.
IPR000806. RabGDI.
[Graphical view]
PfamiPF00996. GDI. 1 hit.
[Graphical view]
PRINTSiPR00892. RABGDI.
PR00891. RABGDIREP.

Sequencei

Sequence statusi: Complete.

P39958-1 [UniParc]FASTAAdd to Basket

« Hide

MDQETIDTDY DVIVLGTGIT ECILSGLLSV DGKKVLHIDK QDHYGGEAAS    50
VTLSQLYEKF KQNPISKEER ESKFGKDRDW NVDLIPKFLM ANGELTNILI 100
HTDVTRYVDF KQVSGSYVFK QGKIYKVPAN EIEAISSPLM GIFEKRRMKK 150
FLEWISSYKE DDLSTHQGLD LDKNTMDEVY YKFGLGNSTK EFIGHAMALW 200
TNDDYLQQPA RPSFERILLY CQSVARYGKS PYLYPMYGLG ELPQGFARLS 250
AIYGGTYMLD TPIDEVLYKK DTGKFEGVKT KLGTFKAPLV IADPTYFPEK 300
CKSTGQRVIR AICILNHPVP NTSNADSLQI IIPQSQLGRK SDIYVAIVSD 350
AHNVCSKGHY LAIISTIIET DKPHIELEPA FKLLGPIEEK FMGIAELFEP 400
REDGSKDNIY LSRSYDASSH FESMTDDVKD IYFRVTGHPL VLKQRQEQEK 450
Q 451
Length:451
Mass (Da):51,206
Last modified:February 1, 1995 - v1
Checksum:i48CF8D45FC031772
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S69371 Genomic DNA. Translation: AAB30540.1.
U18916 Genomic DNA. Translation: AAC03234.1.
BK006939 Genomic DNA. Translation: DAA07795.1.
PIRiS44446.
RefSeqiNP_011062.1. NM_001179026.1.

Genome annotation databases

EnsemblFungiiYER136W; YER136W; YER136W.
GeneIDi856876.
KEGGisce:YER136W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S69371 Genomic DNA. Translation: AAB30540.1 .
U18916 Genomic DNA. Translation: AAC03234.1 .
BK006939 Genomic DNA. Translation: DAA07795.1 .
PIRi S44446.
RefSeqi NP_011062.1. NM_001179026.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1UKV X-ray 1.50 G 1-451 [» ]
2BCG X-ray 1.48 G 1-451 [» ]
3CPH X-ray 2.90 G/H 1-451 [» ]
3CPI X-ray 2.30 G/H 1-451 [» ]
3CPJ X-ray 2.35 G 1-451 [» ]
ProteinModelPortali P39958.
SMRi P39958. Positions 5-446.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36882. 35 interactions.
DIPi DIP-785N.
IntActi P39958. 16 interactions.
MINTi MINT-502785.
STRINGi 4932.YER136W.

2D gel databases

UCD-2DPAGE P39958.

Proteomic databases

MaxQBi P39958.
PaxDbi P39958.
PeptideAtlasi P39958.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER136W ; YER136W ; YER136W .
GeneIDi 856876.
KEGGi sce:YER136W.

Organism-specific databases

CYGDi YER136w.
SGDi S000000938. GDI1.

Phylogenomic databases

eggNOGi COG5044.
GeneTreei ENSGT00530000063044.
HOGENOMi HOG000163825.
KOi K17255.
OMAi KKYGNYN.
OrthoDBi EOG72C58R.

Enzyme and pathway databases

BioCyci YEAST:G3O-30297-MONOMER.

Miscellaneous databases

EvolutionaryTracei P39958.
NextBioi 983252.
PROi P39958.

Gene expression databases

Genevestigatori P39958.

Family and domain databases

InterProi IPR018203. GDP_dissociation_inhibitor.
IPR000806. RabGDI.
[Graphical view ]
Pfami PF00996. GDI. 1 hit.
[Graphical view ]
PRINTSi PR00892. RABGDI.
PR00891. RABGDIREP.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "GDI1 encodes a GDP dissociation inhibitor that plays an essential role in the yeast secretory pathway."
    Garrett M.D., Zahner J.E., Cheney C.M., Novick P.J.
    EMBO J. 13:1718-1728(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Characterization of the ORF YBR264c in Saccharomyces cerevisiae, which encodes a new yeast Ypt that is degraded by a proteasome-dependent mechanism."
    Louvet O., Roumanie O., Barthe C., Peypouquet M.-F., Schaeffer J., Doignon F., Crouzet M.
    Mol. Gen. Genet. 261:589-600(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH YPT10.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Structure of Rab GDP-dissociation inhibitor in complex with prenylated YPT1 GTPase."
    Rak A., Pylypenko O., Durek T., Watzke A., Kushnir S., Brunsveld L., Waldmann H., Goody R.S., Alexandrov K.
    Science 302:646-650(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) IN COMPLEX WITH YPT1.

Entry informationi

Entry nameiGDI1_YEAST
AccessioniPrimary (citable) accession number: P39958
Secondary accession number(s): D3DM41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7280 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

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