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P39958 (GDI1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Rab GDP-dissociation inhibitor

Short name=Rab GDI
Alternative name(s):
Secretory pathway GDP dissociation inhibitor
Gene names
Name:GDI1
Synonyms:SEC19
Ordered Locus Names:YER136W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length451 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulates the GDP/GTP exchange reaction of SEC4 by inhibiting the dissociation of GDP from it, and the subsequent binding of GTP to SEC4. Plays an essential role in the yeast secretory pathway. Ref.1

Subunit structure

Interacts with the GDP-bound form of Rab GTPase YPT1. Interacts with YPT10. Ref.4

Subcellular location

Cytoplasm Ref.5.

Miscellaneous

Present with 7280 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the Rab GDI family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 451451Rab GDP-dissociation inhibitor
PRO_0000056684

Regions

Region106 – 1127Interaction with YPT1
Region234 – 25926Interaction with YPT1

Secondary structure

............................................................................................... 451
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P39958 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 48CF8D45FC031772

FASTA45151,206
        10         20         30         40         50         60 
MDQETIDTDY DVIVLGTGIT ECILSGLLSV DGKKVLHIDK QDHYGGEAAS VTLSQLYEKF 

        70         80         90        100        110        120 
KQNPISKEER ESKFGKDRDW NVDLIPKFLM ANGELTNILI HTDVTRYVDF KQVSGSYVFK 

       130        140        150        160        170        180 
QGKIYKVPAN EIEAISSPLM GIFEKRRMKK FLEWISSYKE DDLSTHQGLD LDKNTMDEVY 

       190        200        210        220        230        240 
YKFGLGNSTK EFIGHAMALW TNDDYLQQPA RPSFERILLY CQSVARYGKS PYLYPMYGLG 

       250        260        270        280        290        300 
ELPQGFARLS AIYGGTYMLD TPIDEVLYKK DTGKFEGVKT KLGTFKAPLV IADPTYFPEK 

       310        320        330        340        350        360 
CKSTGQRVIR AICILNHPVP NTSNADSLQI IIPQSQLGRK SDIYVAIVSD AHNVCSKGHY 

       370        380        390        400        410        420 
LAIISTIIET DKPHIELEPA FKLLGPIEEK FMGIAELFEP REDGSKDNIY LSRSYDASSH 

       430        440        450 
FESMTDDVKD IYFRVTGHPL VLKQRQEQEK Q 

« Hide

References

« Hide 'large scale' references
[1]"GDI1 encodes a GDP dissociation inhibitor that plays an essential role in the yeast secretory pathway."
Garrett M.D., Zahner J.E., Cheney C.M., Novick P.J.
EMBO J. 13:1718-1728(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Characterization of the ORF YBR264c in Saccharomyces cerevisiae, which encodes a new yeast Ypt that is degraded by a proteasome-dependent mechanism."
Louvet O., Roumanie O., Barthe C., Peypouquet M.-F., Schaeffer J., Doignon F., Crouzet M.
Mol. Gen. Genet. 261:589-600(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH YPT10.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Structure of Rab GDP-dissociation inhibitor in complex with prenylated YPT1 GTPase."
Rak A., Pylypenko O., Durek T., Watzke A., Kushnir S., Brunsveld L., Waldmann H., Goody R.S., Alexandrov K.
Science 302:646-650(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) IN COMPLEX WITH YPT1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S69371 Genomic DNA. Translation: AAB30540.1.
U18916 Genomic DNA. Translation: AAC03234.1.
BK006939 Genomic DNA. Translation: DAA07795.1.
PIRS44446.
RefSeqNP_011062.1. NM_001179026.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1UKVX-ray1.50G1-451[»]
2BCGX-ray1.48G1-451[»]
3CPHX-ray2.90G/H1-451[»]
3CPIX-ray2.30G/H1-451[»]
3CPJX-ray2.35G1-451[»]
ProteinModelPortalP39958.
SMRP39958. Positions 5-446.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36882. 35 interactions.
DIPDIP-785N.
IntActP39958. 16 interactions.
MINTMINT-502785.
STRING4932.YER136W.

2D gel databases

UCD-2DPAGEP39958.

Proteomic databases

MaxQBP39958.
PaxDbP39958.
PeptideAtlasP39958.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYER136W; YER136W; YER136W.
GeneID856876.
KEGGsce:YER136W.

Organism-specific databases

CYGDYER136w.
SGDS000000938. GDI1.

Phylogenomic databases

eggNOGCOG5044.
GeneTreeENSGT00530000063044.
HOGENOMHOG000163825.
KOK17255.
OMAKKYGNYN.
OrthoDBEOG72C58R.

Enzyme and pathway databases

BioCycYEAST:G3O-30297-MONOMER.

Gene expression databases

GenevestigatorP39958.

Family and domain databases

InterProIPR018203. GDP_dissociation_inhibitor.
IPR000806. RabGDI.
[Graphical view]
PfamPF00996. GDI. 1 hit.
[Graphical view]
PRINTSPR00892. RABGDI.
PR00891. RABGDIREP.
ProtoNetSearch...

Other

EvolutionaryTraceP39958.
NextBio983252.
PROP39958.

Entry information

Entry nameGDI1_YEAST
AccessionPrimary (citable) accession number: P39958
Secondary accession number(s): D3DM41
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references