Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA damage-responsive transcriptional repressor RPH1

Gene

RPH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional repressor of photolyase PHR1. Recognizes and binds the sequence AG4 in the upstream repressing sequence of PHR1. Derepresses PHR1 transcription when phosphorylated.2 Publications

Miscellaneous

Present with 2229 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri709 – 732C2H2-type 1PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri738 – 763C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST26

GO - Molecular functioni

  • histone demethylase activity (H3-K36 specific) Source: SGD
  • histone demethylase activity (H3-K9 specific) Source: SGD
  • metal ion binding Source: UniProtKB-KW
  • sequence-specific DNA binding Source: SGD
  • transcriptional repressor activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: SGD

GO - Biological processi

  • histone demethylation Source: SGD
  • negative regulation of autophagy Source: SGD
  • negative regulation of transcription by RNA polymerase II Source: SGD
  • positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30330-MONOMER
BRENDAi1.14.11.27 984
ReactomeiR-SCE-3214842 HDMs demethylate histones
R-SCE-5625886 Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3
R-SCE-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks

Names & Taxonomyi

Protein namesi
Recommended name:
DNA damage-responsive transcriptional repressor RPH1
Gene namesi
Name:RPH1
Ordered Locus Names:YER169W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER169W
SGDiS000000971 RPH1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000468491 – 796DNA damage-responsive transcriptional repressor RPH1Add BLAST796

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei399PhosphothreonineCombined sources1
Modified residuei430PhosphoserineCombined sources1
Modified residuei459PhosphoserineCombined sources1
Modified residuei557PhosphoserineCombined sources1
Modified residuei561PhosphoserineCombined sources1
Modified residuei575PhosphoserineCombined sources1
Modified residuei584PhosphoserineCombined sources1
Modified residuei652PhosphoserineCombined sources1
Modified residuei689PhosphoserineCombined sources1

Post-translational modificationi

RAD53-dependent phosphorylated in response to DNA damage.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39956
PaxDbiP39956
PRIDEiP39956

PTM databases

iPTMnetiP39956

Interactioni

Protein-protein interaction databases

BioGridi36922, 140 interactors
DIPiDIP-5418N
IntActiP39956, 9 interactors
STRINGi4932.YER169W

Structurei

Secondary structure

1796
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 17Combined sources9
Helixi21 – 24Combined sources4
Helixi27 – 32Combined sources6
Helixi35 – 38Combined sources4
Beta strandi41 – 46Combined sources6
Helixi50 – 53Combined sources4
Helixi62 – 67Combined sources6
Beta strandi69 – 72Combined sources4
Beta strandi74 – 91Combined sources18
Beta strandi95 – 97Combined sources3
Helixi98 – 104Combined sources7
Turni105 – 107Combined sources3
Helixi142 – 148Combined sources7
Turni149 – 151Combined sources3
Helixi157 – 160Combined sources4
Helixi162 – 174Combined sources13
Turni175 – 178Combined sources4
Beta strandi182 – 188Combined sources7
Beta strandi201 – 203Combined sources3
Beta strandi222 – 226Combined sources5
Beta strandi231 – 235Combined sources5
Helixi238 – 240Combined sources3
Beta strandi242 – 251Combined sources10
Beta strandi253 – 257Combined sources5
Helixi260 – 262Combined sources3
Helixi263 – 272Combined sources10
Helixi275 – 278Combined sources4
Turni285 – 287Combined sources3
Beta strandi290 – 292Combined sources3
Helixi294 – 298Combined sources5
Turni299 – 301Combined sources3
Beta strandi305 – 309Combined sources5
Beta strandi314 – 317Combined sources4
Beta strandi323 – 338Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3OPTX-ray2.20A1-373[»]
3OPWX-ray2.50A1-373[»]
ProteinModelPortaliP39956
SMRiP39956
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP39956

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 55JmjNPROSITE-ProRule annotationAdd BLAST42
Domaini193 – 355JmjCPROSITE-ProRule annotationAdd BLAST163

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi455 – 471Bipartite nuclear localization signalAdd BLAST17

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri709 – 732C2H2-type 1PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri738 – 763C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST26

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00530000063342
HOGENOMiHOG000112726
InParanoidiP39956
KOiK19799
OMAiYWKTLNF
OrthoDBiEOG092C2SZJ

Family and domain databases

InterProiView protein in InterPro
IPR003347 JmjC_dom
IPR003349 JmjN
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF02373 JmjC, 1 hit
PF02375 JmjN, 1 hit
SMARTiView protein in SMART
SM00558 JmjC, 1 hit
SM00545 JmjN, 1 hit
SM00355 ZnF_C2H2, 2 hits
SUPFAMiSSF57667 SSF57667, 1 hit
PROSITEiView protein in PROSITE
PS51184 JMJC, 1 hit
PS51183 JMJN, 1 hit
PS00028 ZINC_FINGER_C2H2_1, 1 hit
PS50157 ZINC_FINGER_C2H2_2, 2 hits

Sequencei

Sequence statusi: Complete.

P39956-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKLIAPSEI VGGVPVFKPT YEQFEDFYAY CKAINKYGMK SGVVKVIPPK
60 70 80 90 100
EWKDKLDLPY SAETLQKIKI KSPIQQHISG NKGLFMVQNV EKNKTYNIIQ
110 120 130 140 150
WKDLSKDYVP PEDPKARRNS RKGSVSKSTK LKLKNFESSF NIDDFEQFRT
160 170 180 190 200
EYTIDLSDFQ NTERLKFLEE YYWKTLNFTT PMYGADTPGS IFPEGLNVWN
210 220 230 240 250
VAKLPNILDH METKVPGVND SYLYAGLWKA SFSWHLEDQD LYSINYIHFG
260 270 280 290 300
APKQWYSIPQ EDRFKFYKFM QEQFPEEAKN CPEFLRHKMF LASPKLLQEN
310 320 330 340 350
GIRCNEIVHH EGEFMITYPY GYHAGFNYGY NLAESVNFAL EEWLPIGKKA
360 370 380 390 400
GKCHCISDSV EIDVKKLAKS WRDNNKESKG TPPLNQLPNP AMPLLHRPTL
410 420 430 440 450
KEMESSSLRS TSPDVGHFSN FKSKSSGVSS PLLSRMKDYS NIVEPTLEDP
460 470 480 490 500
TLKLKRISSF QEQPLNKLLK RETSQTAMLT DHEDNIVAMS LTSMANSAAS
510 520 530 540 550
SPRLPLSRLN SSNELSNAQP LLDMTNNTLA FPRPNGPSGL NPLLYISNKN
560 570 580 590 600
ISGISHSAPH SPVNPNISLI KRVKSPNIVT LNISRESSRS PIALNYEARQ
610 620 630 640 650
QHSQQHSFST PSTVSNLSTS VLGPLSDTND IKTPHPERPN HKTANRILKK
660 670 680 690 700
ESPVETSKSN LILSKVASTR QEDSFTSRND DLDKEQGSSP LNSKFAPEEI
710 720 730 740 750
VLSGKNKIYI CKECQRKFSS GHHLTRHKKS VHSGEKPHSC PKCGKRFKRR
760 770 780 790
DHVLQHLNKK IPCISNETTV DAPIMNPTVQ PQDGKAAINQ QSTPLN
Length:796
Mass (Da):90,211
Last modified:February 1, 1995 - v1
Checksum:i606C27836E1600F5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18922 Genomic DNA Translation: AAB64696.1
BK006939 Genomic DNA Translation: DAA07831.1
PIRiS50672
RefSeqiNP_011096.1, NM_001179059.1

Genome annotation databases

EnsemblFungiiYER169W; YER169W; YER169W
GeneIDi856916
KEGGisce:YER169W

Similar proteinsi

Entry informationi

Entry nameiRPH1_YEAST
AccessioniPrimary (citable) accession number: P39956
Secondary accession number(s): D3DM77
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 28, 2018
This is version 163 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health