P39955 (SAP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein SAP1

Alternative name(s):
SIN1-associated protein

Gene names

Name:	SAP1
Ordered Locus Names:	YER047C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	897 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with SPT2/SIN1.
Miscellaneous	Present with 300 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the AAA ATPase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ATP catabolic process Inferred from sequence or structural similarity Ref.3. Source: GOC
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity Inferred from sequence or structural similarity Ref.3. Source: SGD protein binding Inferred from physical interaction PubMed 11743162PubMed 19841731. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
HOF1	Q05080	4	EBI-16463,EBI-5412
MYO3	P36006	3	EBI-16463,EBI-11670
MYO5	Q04439	4	EBI-16463,EBI-11687

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 897	897	Protein SAP1		PRO_0000084761
Regions
	Nucleotide binding	645 – 652	8	ATP Potential
Amino acid modifications
	Modified residue	536	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P39955 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: E9780B269700144C	FASTA	897	100,332
	10 20 30 40 50 60 MDSQRSHHIL TRLTKIRRRP QQPLTDFTEL YSRIANETIY YLNLEEKKRY KEALQGWKAL 70 80 90 100 110 120 TTDVLFKQTL IEHNYPNTQS YTKDEVSLQN GIRELYHKSV MHLKRVKKLV REEPAPRNDM 130 140 150 160 170 180 PSSKTYTNHS SSFTRSTEPP PVFQMVPGRM MKTLRNRNAC GYKTAYSNPS LSSYGNSTSI 190 200 210 220 230 240 KRGEDAENIR VNFVPSKPLS NNASRQHKNP IEHNDPPLKK ETELYSDKYI SEPILIDLTN 250 260 270 280 290 300 DEDDHDVGIL KGHNVFDEEE SDGFEFDVSD YYDNFSEVDV EEEEEEKEER RRIKTLEAIQ 310 320 330 340 350 360 QQMSDLSVTS STSSNKSVSS SENVPGSCIQ SLPTTAPALP SLPPPPLLNV DRASSTGALK 370 380 390 400 410 420 PHSLETSTTM DSSKIRNPQI SKLMKNNHVP YLKGTKSTPT LITKSTPTFI TRSKSNTKPI 430 440 450 460 470 480 IKSNASSPTS SLTVPNSVIQ KPKTAAMAAK RVLNSKKVAS NPALNTTKKS HPILKSKTAK 490 500 510 520 530 540 VPNSSSKKTS SHPSRPVSNS KPYSHGASQN KKPSKNQTTS MSKTNRKIPA QKKIGSPKIE 550 560 570 580 590 600 DVGTEDATEH ATSLNEQREE PEIDKKVLRE ILEDEIIDSL QGVDRQAAKQ IFAEIVVHGD 610 620 630 640 650 660 EVHWDDIAGL ESAKYSLKEA VVYPFLRPDL FRGLREPVRG MLLFGPPGTG KTMLARAVAT 670 680 690 700 710 720 ESHSTFFSIS ASSLTSKYLG ESEKLVRALF AIAKKLSPSI IFVDEIDSIM GSRNNENENE 730 740 750 760 770 780 SSRRIKNEFL VQWSSLSSAA AGSNKSNTNN SDTNGDEDDT RVLVLAATNL PWSIDEAARR 790 800 810 820 830 840 RFVRRQYIPL PEDQTRHVQF KKLLSHQKHT LTESDFDELV KITEGYSGSD ITSLAKDAAM 850 860 870 880 890 GPLRDLGDKL LETEREMIRP IGLVDFKNSL VYIKPSVSQD GLVKYEKWAS QFGSSGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The reference genome sequence of Saccharomyces cerevisiae: Then and now." Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M. G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Association of yeast SAP1, a novel member of the 'AAA' ATPase family of proteins, with the chromatin protein SIN1." Liberzon A., Shpungin S., Bangio H., Yona E., Katcoff D.J. FEBS Lett. 388:5-10(1996) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: ADR376.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]	"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution." Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O. Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-536, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18796 Genomic DNA. Translation: AAB64582.1. BK006939 Genomic DNA. Translation: DAA07702.1.
PIR	S50550.
RefSeq	NP_010966.1. NM_001178938.1.
3D structure databases
ProteinModelPortal	P39955.
SMR	P39955. Positions 559-889.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	36784. 33 interactions.
DIP	DIP-1550N.
IntAct	P39955. 15 interactions.
MINT	MINT-407680.
STRING	4932.YER047C.
Proteomic databases
MaxQB	P39955.
PaxDb	P39955.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER047C; YER047C; YER047C.
GeneID	856771.
KEGG	sce:YER047C.
Organism-specific databases
CYGD	YER047c.
SGD	S000000849. SAP1.
Phylogenomic databases
eggNOG	COG0464.
GeneTree	ENSGT00570000078874.
HOGENOM	HOG000216613.
OMA	HGDEVHW.
OrthoDB	EOG72JWRT.
Enzyme and pathway databases
BioCyc	YEAST:G3O-30226-MONOMER.
Gene expression databases
Genevestigator	P39955.
Family and domain databases
Gene3D	3.40.50.300. 1 hit.
InterPro	IPR003593. AAA+_ATPase. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR027417. P-loop_NTPase. [Graphical view]
Pfam	PF00004. AAA. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982962.

Entry information

Entry name

SAP1_YEAST

Accession

Primary (citable) accession number: P39955
Secondary accession number(s): D3DLU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	June 11, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program